The BCCM/LMBP Plasmid Collection accepts plasmids from and distributes plasmids to researchers worldwide. Funding by the Belgian Science Policy (Belspo) allowed BCCM/LMBP to evolve into a unique plasmid repository in Europe.
BCCM/LMBP is embedded in the Department of Biomedical Molecular Biology (DBMB, Ghent University) and the VIB-UGent Center for Inflammation Research (IRC, VIB), which together form the host laboratory for BCCM/LMBP.
The BCCM/LMBP activities are continuously supported by the research activities of its host laboratory, which focus on molecular and cellular mechanisms that are at the origin of chronic inflammation, autoimmune diseases, infectious diseases and cancer. These mechanisms are mainly studied in cellular and animal model systems by using the most advanced methods of molecular biology, cell biology and immunology. Expression plasmids for human and mouse genes are frequently generated and extensively used, offering an ideal environment for BCCM/LMBP. Research of the host laboratory is multidisciplinary with molecular biologists, immunologists, cell biologists, and bioinformaticians collaborating with clinicians. It is the ambition of the host laboratory to translate basic research into innovative ways for diagnosis and therapy of human diseases.
Click here for a list of publications of the host laboratory.
BCCM/LMBP has already performed a number of research projects to improve plasmid DNA storage and to enlarge its scope of basic vectors and genes in view of the needs of the academic and industrial communities, and is continuously interested in project-based scientific collaborations on these issues.
Scope of the biological material
The BCCM/LMBP Plasmid Collection warrants the long-term storage and distribution of plasmids, microbial host strains and DNA libraries of fundamental, biotechnological, educational or general scientific importance.
The focus is on the collection of recombinant plasmids that can replicate in a microbial host strain, with main interest for:
- empty cloning plasmids useful for recombinant (over)expression of proteins of interest in bacteria, yeast, fungi, insect cells, plant and animal cells;
- (expression) plasmids carrying specific protein-coding genes and derivatives from a wide range of organisms (human, mouse, various bacterial species, ...);
- expression plasmids with specific non-coding genes such as shRNA and miRNA;
- plasmids for mouse genetic engineering, allowing the generation of transgenic mice.
BCCM/LMBP also accepts natural and genetically modified animal or human cell lines, including hybridomas, as well as other genetic material, in the safe deposit and patent deposit collections.
BCCM/LMBP is ISO 9001:2008 certified for accession, control, preservation, storage and supply of biological material and related information in the frame of public deposits, safe deposits and patent deposits.
By default, BCCM/LMBP provides plasmids, plasmid hosts and DNA libraries of the public collections under the conditions of the BCCM Material Transfer Agreement (MTA). Exceptionally, another MTA is applicable.
Additionally, BCCM/LMBP provides easy access to the MISSION TRC1 and TRC1.5 shRNA libraries to all researchers of Ghent University, researchers of VIB and to researchers of other institutes in the framework of a collaboration with either a Ghent University or VIB laboratory.
BCCM/LMBP accepts plasmids, plasmid hosts and DNA libraries for public deposits.
Lastly, BCCM/LMBP offers storage of third party’s biological material for all of the biological resources mentioned above.
BCCM/LMBP provides a number of scientific services, such as characterisation tests (plasmid profile analysis, sequence analysis of non-characterised plasmids,...).