Holding
The BCCM/LMG public collection maintains over 22.000
strains, representing some 380 genera and 2.700 species, subspecies or pathovars,
encompassing plant associated and phytopathogenic bacteria (pseudomonads, xanthomonads,
erwiniae, agrobacteria, coryneforms, etc.), bacteria of medical or veterinary
importance (Arcobacter, Campylobacter, Helicobacter, aeromonads, flavobacteria,
bordetellae, enterococci, streptococci), marine bacteria (Vibrionaceae)
and various groups of biotechnological interest (such as lactic acid bacteria
and acetic acid bacteria, N2 fixers, clostridia, bacilli, streptomycetes, agrobacteria,
etc.).
Most of the commonly used control, test and bioassay strains are incorporated.
BCCM/LMG also distributes the complete experimental strain panel of the Burkholderia
cepacia complex.
Services
All assignments, with exception of deposits of microbial resources for public access, are treated strictly confidential.
- Deposit of strains for public access:
before sending strains, it is advisable to contact BCCM/LMG to make sure
that the material to be deposited is within its scope and technical capability.
Upon deposit, the depositor must complete the corresponding BCCM/LMG
Public deposit Accession Form.
Deposits of strains for public access are without costs for the depositor.
- Safe deposit:
before sending strains, it is advisable to contact BCCM/LMG to make sure
that the material to be deposited is within its scope and technical
capabilities.
Upon deposit, the depositor must complete the corresponding BCCM/LMG
Contract Form. Two options are possible: either the material is deposited
for an undefined period (that can be terminated by the depositor each year
up to one month before a next year starts), or the material is deposited for
a predefined period (that can be extended), see the BCCM/LMG
Extension/Termination Form. In the latter case, an attestation of deposit
can be issued.
For related costs see pricelist.
- Patent deposit:
before sending strains,
it is advisable to contact BCCM/LMG to make sure that the material to be
deposited is within its scope and technical capabilities.
Upon deposit, the depositor must complete the international Accession Form
BCCM/LMG/BP/1 as well as the bilateral
contract Form BCCM/LMG/DBT1.
The actual deposit implies the furnishing of 23 freeze-dried samples from
a same batch culture. From one or more of these, BCCM/LMG will check viability
and purity, and prepare an additional stock of 20 cryopreserved samples.
Alternatively, the depositor can provide only 3 samples (active or freeze-dried)
from which BCCM/LMG will check viability and prepare a stock of 20 cryopreserved
samples as well as 20 freeze-dried samples at extra charges.
Once BCCM/LMG has accepted the organism and confirmed its viability and
purity, it will send to the depositor or to the party designated on form
BP/1, the completed international Forms BCCM/LMG/BP/4 (receipt of an original
deposit) and BCCM/LMG/BP/9 (viability statement). These documents constitute
the proofs of the deposit.
See also the detailed Manual for the deposit
of Biological Material for Patent Purposes under the Budapest Treaty.
For related costs see pricelist.
- Storage of third party’s biological material:
BCCM/LMG accepts for storage of third party’s biological material any kind of biological material, provided it does not belong to a biohazard group higher than 2, which can be cryopreserved over liquid nitrogen.
Before sending material, it is advisable to contact BCCM/LMG to make sure that the material to be stored is within its scope and technical capability.
For related costs see pricelist.
- Distribution of strains:
Strains of the public collection are accessible to the
scientific community under the conditions of the BCCM Material
Transfer Agreement (MTA), if necessary amended with additional conditions
possibly already attached to the biological material.
For more information on ordering microbial resources click here.
Consult the BCCM/LMG catalogue to
search the strains you require.
Strains of the safe deposit collection are not catalogued
and are only available to the depositor or to third parties with the written
authorization of the depositor. For related costs see pricelist.
The distribution of samples of strains deposited under the Budapest
Treaty for patent purposes depends on the authorization by
the depositor or an entitled Industrial Property Office. Strains are not
catalogued.
For related costs see pricelist.
Accession, control, preservation, storage and supply
of the biological material are ISO
9001/2008 certified.
- Freeze-drying service:
Bacterial cultures of third parties can be submitted for freeze-drying at
BCCM/LMG, provided they do not belong to a biohazard group higher than 2,
and can be cultivated and freeze-dried using the current standard BCCM/LMG
protocols. Batches of 10, 20 or more ampoules can be prepared and after
confirmation of viability and purity, the complete lot is forwarded to the
customer.
For related costs see pricelist.
- Identification, characterization and molecular
typing bacterial cultures:
A broad range of methods is available for the typing, characterization and/or
identification of bacteria:
Fenotypic techniques: conventional testing using API strips, Biolog
breathprinting, electrophoresis of cellular proteins, gaschromatographic
analysis of cellular fatty acids;
Genomic techniques: DNA fingerprinting (rep-PCR, RAPD, PFGE), realtime
PCR, sequence analysis of 16S rDNA, multilocus sequence analysis of house
keeping genes, determination of the mol% G+C content of DNA, DNA:DNA hybridizations.
Techniques are chosen case by case based on a number of preliminar tests
and taking into account the aim of the assignment.
International as well as elaborated in-house databases and performant
software are used for optimal data processing and interpretation.
Reports can be either summarized or extended according to the instructions
of the customer.
A cost estimate is made on request.
Research interests
Research projects developed by collection staff and research groups of the
host laboratory are largely focussed on a polyphasic approach (using complementary
molecular characterization) aiming at the phylogenetic study, the identification
and the fine-typing of various bacterial groups, in the frame of:
- Assessing microbial diversity in a wide range of
environments
- Bioprospecting and screening
- Phytobacteriology and bacteriological aspects of
sustainable agriculture, agroforestry and aquaculture
- Microbial ecology, symbiotic systems, natural soil
bacteria
- Food bacteriology
- Antibiotic acquired resistance, epidemiology of
clinical isolates from man or animal
- Biodegradation and composting, biodeterioration
- Bioremediation
Organisation chart
Further information & contact
Promoter: Prof. Dr Paul De Vos
Manager: Dr Danielle Janssens
Public Collection Curator: Ir Claudine Vereecke
Contact:
Dr Danielle Janssens
BCCM/LMG
Laboratorium voor Microbiologie, Universiteit Gent (UGent)
K.L. Ledeganckstraat 35
B-9000 Gent (map
& directions)
Phone: +32-(0)9-264.51.08
Fax: +32-(0)9-264.53.46
E-mail: BCCM.LMG@UGent.be
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BCCM/LMG Bacteria Collection