LOCUS       pGB-dummy 11;12         2709 bp ds-DNA     circular SYN 29-JUL-2016
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 28, 2019 from SnapGene Viewer 5.0.6 to Vector
            NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|776649600|
COMMENT     VNTDBDATE|776649600|
COMMENT     VNTNAME|pGB-dummy 11;12|
FEATURES             Location/Qualifiers
     misc_binding    340..362
                     /label=LacO
     primer_bind     370..391
                     /label=SP6
     misc_feature    403..408
                     /label=BsaI site
     misc_feature    410..413
                     /label=overhang 11
     misc_feature    414..436
                     /label=dummy
     misc_feature    437..440
                     /label=overhang 12
     misc_feature    complement(442..447)
                     /label=BsaI site(1)
                     /label=BsaI site
     primer_bind     complement(457..477)
                     /label=T7
     CDS             565..633
                     /label=LacZ alpha
     CDS             1072..1932
                     /label=Beta lactamase
     rep_origin      2027..2709
                     /label=ColE1 origin
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
       61 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      361 tttcacacga gctatttagg tgacactata gaagtgaagc ttggtctcaa gaaggctgag
      421 taggcaagat gttctggttg tgagaccgaa ttctcgccct atagtgagtc gtattacctg
      481 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      541 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      601 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
      661 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      721 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      781 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
      841 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
      901 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgaggtatc
     1801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2701 aacgcggcc
//
