LOCUS       pGBdest_ccdB            4958 bp ds-DNA     circular SYN 28-NOV-2019
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 28, 2019 from SnapGene Viewer 5.0.6 to Vector
            NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|895449600|
COMMENT     VNTDBDATE|895449600|
COMMENT     VNTNAME|pGBdest_ccdB|
FEATURES             Location/Qualifiers
     misc_feature    1..534
                     /label=GmR
     misc_feature    860..863
                     /label=overhang 01
     misc_feature    complement(865..870)
                     /label=BsaI site(1)
                     /label=BsaI site
     CDS             complement(914..1165)
                     /codon_start=1
                     /transl_table=11
                     /gene="lacZ alpha"
                     /product="beta-galactosidase alpha peptide"
                     /label=beta-galactosidase alpha peptide
                     /db_xref="GI:568816423"
                     /protein_id="AHE38481.1"
     CDS             complement(1184..1489)
                     /codon_start=1
                     /transl_table=11
                     /gene="ccdB"
                     /product="CcdB"
                     /label=CcdB
                     /db_xref="GI:568816422"
                     /protein_id="AHE38480.1"
     CDS             complement(1508..2167)
                     /codon_start=1
                     /transl_table=11
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /db_xref="GI:568816421"
                     /protein_id="AHE38479.1"
     misc_feature    2298..2303
                     /label=BsaI site
     misc_feature    2305..2308
                     /label=overhang 16
     misc_feature    2317..2482
                     /label=Tn7L
     CDS             complement(2838..3653)
                     /label=KanR
     rep_origin      3699..4381
                     /label=ColE1 origin
     misc_feature    4668..4892
                     /label=Tn7R
ORIGIN
        1 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt
       61 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc
      121 accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct
      181 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa
      241 cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag
      301 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg
      361 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga
      421 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa
      481 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc
      541 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
      601 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
      661 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
      721 acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg
      781 gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg
      841 cgaacgagcg caaggtttca ccttgagacc gaattcaaaa aaaagcggac ctccgaagag
      901 gtccgccaga tgatcatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
      961 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     1021 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     1081 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     1141 gacgttgtaa aacgacggcc agcattgatt acctcctact agtttatatt ccccagaaca
     1201 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     1261 cgataacgga gactggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     1321 cgatatgcac cactgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg
     1381 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     1441 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcata gctgtcctcc
     1501 tgagctctta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1561 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1621 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1681 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1741 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1801 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1861 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1921 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1981 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     2041 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     2101 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     2161 tctccatagc tgacctccta agcttagcac acctcttttt gacatacttc gggtatacat
     2221 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
     2281 ttttagtaag cctcgagggt ctcagtatgt ttaaacaacc agataagtga aatctagttc
     2341 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     2401 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     2461 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
     2521 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
     2581 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
     2641 atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2701 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2761 gctttcttcc cttcctttct cgccacgttc gccaaacgta ctgcggtaaa gctcatcagc
     2821 gtggtcgtga agaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     2881 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     2941 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     3001 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     3061 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     3121 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     3181 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     3241 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     3301 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     3361 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     3421 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     3481 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     3541 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     3601 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc
     3661 cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg
     3721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3901 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4441 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4501 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     4561 ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc gtaacctggc
     4621 aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa
     4681 taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca
     4741 gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt
     4801 tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg
     4861 gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt accgaacaac
     4921 tccgcggccg ggaagccgat ctcggcttga acgaattg
//
