LOCUS       pCi-C-TagRFP-DEST         6404 bp    DNA     circular     9-MAY-2016
DEFINITION  LMBP 9841.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11084653|
COMMENT     VNTNAME|pCi-C-TagRFP-DEST|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
FEATURES             Location/Qualifiers
     promoter        138..721
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        1034..1050
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(1067..1191)
                     /vntifkey="0"
                     /label=attR1
     promoter        1195..1251
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             1300..1959
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2280..2585
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2626..2750
                     /vntifkey="0"
                     /label=attR2
     terminator      complement(3563..3659)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3563..3659
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      3818..4273
                     /vntifkey="33"
                     /label=f1\ori
     CDS             4712..5572
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      6113..6391
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             2782..3474
                     /vntifkey="4"
                     /label=TagRFP
BASE COUNT     1680 a      1523 c      1550 g      1651 t 
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 
     1021 aggctagagt acttaatacg actcactata ggctagcctc gagatcacaa gtttgtacaa 
     1081 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 
     1141 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca 
     1201 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt 
     1261 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 
     1321 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 
     1381 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 
     1441 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 
     1501 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 
     1561 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 
     1621 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 
     1681 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 
     1741 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 
     1801 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 
     1861 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa 
     1921 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta 
     1981 aaagccagat aacagtatgc gtatttgcgc gctcgcgaac cggtgtatac ccgaagtatg 
     2041 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc 
     2101 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa 
     2161 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga 
     2221 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa 
     2281 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac 
     2341 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc 
     2401 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct 
     2461 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg 
     2521 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa 
     2581 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata 
     2641 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg 
     2701 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatctcgag 
     2761 aacttcacgc gtggtacccc ggaagagctg attaaggaga acatgcacat gaagctgtac 
     2821 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc 
     2881 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc 
     2941 ttcgacatcc tggctaccag cttcatgtac ggcagcagaa ccttcatcaa ccacacccag 
     3001 ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc 
     3061 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc 
     3121 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag 
     3181 aagaaaacac tcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg 
     3241 gaaggcagaa ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttc 
     3301 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat 
     3361 gtggaccaca gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac 
     3421 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa ataggctcta 
     3481 gagtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca 
     3541 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 
     3601 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 
     3661 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa 
     3721 atgtggtaaa atcgataagg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg 
     3781 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta 
     3841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 
     3901 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 
     3961 gctctaaatc gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc 
     4021 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt 
     4081 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 
     4141 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 
     4201 tattggttaa aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta 
     4261 acgtttacaa tttcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 
     4321 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 
     4381 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 
     4441 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 
     4501 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 
     4561 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 
     4621 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
     4681 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
     4741 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
     4801 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
     4861 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
     4921 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
     4981 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
     5041 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
     5101 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
     5161 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
     5221 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
     5281 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
     5341 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
     5401 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
     5461 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
     5521 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
     5581 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     5641 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     5701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     5761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     5821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     5881 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     5941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     6001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     6061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     6121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     6181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     6241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     6301 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     6361 cctggccttt tgctggcctt ttgctcacat ggctcgacag atct 
//
