ID   pMC_MultiGene_ZeoR; SV 1; circular; unknown; STD; UNC; 4588 BP.
DT   17-OCT.-2023
DE   LMBP 12797.
CC   Accession number: LMBP 12797
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 17-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        5..561
FT                   /label="ILV5 promoter"
FT   promoter        562..626
FT                   /label="synthetic EM72 promoter"
FT   CDS             627..1001
FT                   /label="zeocin resistance"
FT   terminator      1006..1207
FT                   /label="E. gossypii TEF1 terminator"
FT   CDS             complement(1450..2310)
FT                   /label="ampicillin resistance"
FT   rep_origin      2888..3205
FT                   /label="pMB1 ori"
FT   misc_feature    3210..3378
FT                   /label="ConLS' connector"
FT   promoter        3383..3512
FT                   /label="glpT promoter"
FT   RBS             3513..3548
FT                   /label="synthetic RBS"
FT   CDS             3549..4265
FT                   /label="sfGFP"
FT   terminator      4277..4320
FT                   /label="T1 terminator"
FT   terminator      4354..4394
FT                   /label="T7Te terminator"
FT   misc_feature    4399..4588
FT                   /label="ConRE' connector"
SQ   SEQUENCE  4588 AA; 1418119 MW;
     TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT        60
     GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA       120
     AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT       180
     CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC       240
     CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA       300
     TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT       360
     GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG       420
     CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC       480
     CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA       540
     AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA       600
     CGACAAGGTG TAAGGAGGAC TAAACCATGG CTAAACTCAC CTCTGCTGTT CCAGTCCTGA       660
     CTGCTCGTGA TGTTGCTGGT GCTGTTGAGT TCTGGACTGA TAGACTCGGT TTCTCCCGTG       720
     ACTTCGTAGA GGACGACTTT GCCGGTGTTG TACGTGACGA CGTTACCCTG TTCATCTCCG       780
     CAGTTCAGGA CCAGGTTGTG CCAGACAACA CTCTGGCATG GGTATGGGTT CGTGGTCTGG       840
     ACGAACTGTA CGCTGAGTGG TCTGAGGTCG TGTCTACCAA CTTCCGTGAT GCATCTGGTC       900
     CAGCTATGAC CGAGATCGGT GAACAGCCCT GGGGTCGTGA GTTTGCACTG CGTGATCCAG       960
     CTGGTAACTG CGTGCATTTC GTCGCAGAAG AGCAGGACTA AGCCGAGTAA CTGACAATAA      1020
     AAAGATTCTT GTTTTCAAGA ACTTGTCATT TGTATAGTTT TTTTATATTG TAGTTGTTCT      1080
     ATTTTAATCA AATGTTAGCG TGATTTATAT TTTTTTTCGC CTCGACATCA TCTGCCCAGA      1140
     TGCGAAGTTA AGTGCGCAGA AAGTAATATC ATGCGTCAAT CGTATGTGAA TGCTGGTCGC      1200
     TATACTGGAG TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA      1260
     GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC      1320
     GATCGGTGCG GGCCTCTTCG CTATTACGCC ACCGAGATTA TCAAAAAGGA TCTTCACCTA      1380
     GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG      1440
     GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG      1500
     TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC      1560
     ATCTGGCCCC AGTGCTGCAA TGATACCGCG GGACCCACGC TCACCGGCTC CAGATTTATC      1620
     AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC      1680
     CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG      1740
     TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT      1800
     GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG      1860
     CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT      1920
     GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG      1980
     ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG      2040
     ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT      2100
     AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT      2160
     GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC      2220
     TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT      2280
     AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT      2340
     TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA      2400
     AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GTCATGACCA AAATCCCTTA      2460
     ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG      2520
     AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC      2580
     GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG      2640
     CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA      2700
     GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC      2760
     CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC      2820
     GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA      2880
     CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG      2940
     AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT      3000
     TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA      3060
     GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC      3120
     GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT      3180
     ATCCCCTGAT TCTGTGGATA ACCGTCCCTG AATTCGCATC TAGACTGATG AGACGTGGTA      3240
     GAGCCACAAA CAGCCGGTAC AAGCAACGAT CTCCAGGACC ATCTGAATCA TGCGCGGATG      3300
     ACACGAACTC ACGACGGCGA TCACAGACAT TAACCCACAG TACAGACACT GCGACAACGT      3360
     GGCAATTCGT CGCAATACAA CGGAAAGTGA AACGTGATTT CATGCGTCAT TTTGAACATT      3420
     TTGTAAATCT TATTTAATAA TGTGTGCGGC AATTCACATT TAATTTATGA ATGTTTTCTT      3480
     AACATCGCGG CAACTCAAGA AACGGCAGGT TCGGATCTTA GCTACTAGAG AAAGAGGAGA      3540
     AATACTAGAT GCGTAAAGGC GAAGAGCTGT TCACTGGTGT CGTCCCTATT CTGGTGGAAC      3600
     TGGATGGTGA TGTCAACGGT CATAAGTTTT CCGTGCGTGG CGAGGGTGAA GGTGACGCAA      3660
     CTAATGGTAA ACTGACGCTG AAGTTCATCT GTACTACTGG TAAACTGCCG GTTCCTTGGC      3720
     CGACTCTGGT AACGACGCTG ACTTATGGTG TTCAGTGCTT TGCTCGTTAT CCGGACCATA      3780
     TGAAGCAGCA TGACTTCTTC AAGTCCGCCA TGCCGGAAGG CTATGTGCAG GAACGCACGA      3840
     TTTCCTTTAA GGATGACGGC ACGTACAAAA CGCGTGCGGA AGTGAAATTT GAAGGCGATA      3900
     CCCTGGTAAA CCGCATTGAG CTGAAAGGCA TTGACTTTAA AGAGGACGGC AATATCCTGG      3960
     GCCATAAGCT GGAATACAAT TTTAACAGCC ACAATGTTTA CATCACCGCC GATAAACAAA      4020
     AAAATGGCAT TAAAGCGAAT TTTAAAATTC GCCACAACGT GGAGGATGGC AGCGTGCAGC      4080
     TGGCTGATCA CTACCAGCAA AACACTCCAA TCGGTGATGG TCCTGTTCTG CTGCCAGACA      4140
     ATCACTATCT GAGCACGCAA AGCGTTCTGT CTAAAGATCC GAACGAGAAA CGCGATCATA      4200
     TGGTTCTGCT GGAGTTCGTA ACCGCAGCGG GCATCACGCA TGGTATGGAT GAACTGTACA      4260
     AATGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT      4320
     CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC      4380
     TTTCTGCGTT TATAGCTGGA AATCTGCTCG TCAGTGGTGC TCACACTGAC GAATCATGTA      4440
     CAGATCATAC CGATGACTGC CTGGCGACTC ACAACTAAGC AAGACAGCCG GAACCAGCGC      4500
     CGGCGAACAC CACTGCATAT ATGGCATATC ACAACAGTCC ACGTCTCAAG CAGTTACAGA      4560
     GATGTTACGA ACCACTAGTG CACTGCAG                                         4588
//
