ID   pPTK199_P8_ZeoR; SV 1; circular; unknown; STD; UNC; 2890 BP.
DT   23-OCT-2023
DE   LMBP 12789.
CC   Accession number: LMBP 12789
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   rep_origin      447..764
FT                   /label="pMB1 ori"
FT   promoter        778..831
FT                   /label="PLtetO-1 promoter"
FT   RBS             832..856
FT                   /label="synthetic RBS"
FT   CDS             857..1534
FT                   /label="mRFP1"
FT   terminator      1555..1598
FT                   /label="T1 terminator"
FT   terminator      1632..1672
FT                   /label="T7Te terminator"
FT   promoter        1688..2244
FT                   /label="ILV5 promoter"
FT   promoter        2245..2309
FT                   /label="synthetic EM72 promoter"
FT   CDS             2310..2684
FT                   /label="zeocin resistance"
FT   terminator      2689..2890
FT                   /label="E. gossypii TEF1 terminator"
SQ   SEQUENCE  2890 AA;  893479 MW;
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA        60
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA       120
     AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC       180
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT       240
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC       300
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC       360
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA       420
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG       480
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG       540
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT       600
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT       660
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC       720
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC       780
     CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA       840
     AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA       900
     AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG       960
     TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC      1020
     GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA      1080
     CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG      1140
     TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA      1200
     CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT      1260
     TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG      1320
     TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC      1380
     TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC      1440
     CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA      1500
     ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA      1560
     CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT      1620
     CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC      1680
     TCACCGAATC CTTCAGTAAT GTCTTGTTTC TTTTGTTGCA GTGGTGAGCC ATTTTGACTT      1740
     CGTGAAAGTT TCTTTAGAAT AGTTGTTTCC AGAGGCCAAA CATTCCACCC GTAGTAAAGT      1800
     GCAAGCGTAG GAGAACCAAG ACTGGCATAA ATCAGGTATA AGTGTCGAGC ACTGGCAGGT      1860
     GATCTTCTGA AAGTTTCTAC TAGCAGATAA GATCCAGTAG TCATGCATAT GGCAACAATG      1920
     TACCGTGTGG ATCTAAGAAC GCGTCCTACT AACCTTCGCA TTCGTTGGTC CAGTTTGTTG      1980
     TTATCGATCA ACGTGACAAG GTTGTCGATT CCGCGTAAGC ATGCATACCC AAGGACGCCT      2040
     GTTGCAATTC CAAGTGAGCC AGTTCCAACA ATCTTTGTAA TATTAGAGCA CTTCATTGTG      2100
     TTGCGCTTGA AAGTAAAATG CGAACAAATT AAGAGATAAT CTCGAAACCG CGACTTCAAA      2160
     CGCCAATATG ATGTGCGGCA CACAATAAGC GTTCATATCC GCTGGGTGAC TTTCTCGCTT      2220
     TAAAAAATTA TCCGAAAAAA TTTTCTAGAG TGTTGTTACT TTATACTTCC GGCTCGTATA      2280
     ATACGACAAG GTGTAAGGAG GACTAAACCA TGGCTAAACT CACCTCTGCT GTTCCAGTCC      2340
     TGACTGCTCG TGATGTTGCT GGTGCTGTTG AGTTCTGGAC TGATAGACTC GGTTTCTCCC      2400
     GTGACTTCGT AGAGGACGAC TTTGCCGGTG TTGTACGTGA CGACGTTACC CTGTTCATCT      2460
     CCGCAGTTCA GGACCAGGTT GTGCCAGACA ACACTCTGGC ATGGGTATGG GTTCGTGGTC      2520
     TGGACGAACT GTACGCTGAG TGGTCTGAGG TCGTGTCTAC CAACTTCCGT GATGCATCTG      2580
     GTCCAGCTAT GACCGAGATC GGTGAACAGC CCTGGGGTCG TGAGTTTGCA CTGCGTGATC      2640
     CAGCTGGTAA CTGCGTGCAT TTCGTCGCAG AAGAGCAGGA CTAAGCCGAG TAACTGACAA      2700
     TAAAAAGATT CTTGTTTTCA AGAACTTGTC ATTTGTATAG TTTTTTTATA TTGTAGTTGT      2760
     TCTATTTTAA TCAAATGTTA GCGTGATTTA TATTTTTTTT CGCCTCGACA TCATCTGCCC      2820
     AGATGCGAAG TTAAGTGCGC AGAAAGTAAT ATCATGCGTC AATCGTATGT GAATGCTGGT      2880
     CGCTATACTG                                                             2890
//
