ID   pMC_MultiGene_KanR; SV 1; circular; unknown; STD; UNC; 5027 BP.
DT   17-OCT.-2023
DE   LMBP 12795.
CC   Accession number: LMBP 12795
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 17-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        5..561
FT                   /label="ILV5 promoter"
FT   promoter        562..626
FT                   /label="synthetic EM72 promoter"
FT   CDS             627..1436
FT                   /label="Tn903 neomycin resistance"
FT   terminator      1441..1646
FT                   /label="E. gossypii TEF1 terminator"
FT   CDS             complement(1889..2749)
FT                   /label="ampicillin resistance"
FT   rep_origin      3327..3644
FT                   /label="pMB1 ori"
FT   misc_feature    3649..3817
FT                   /label="ConLS' connector"
FT   promoter        3822..3951
FT                   /label="glpT promoter"
FT   RBS             3952..3987
FT                   /label="synthetic RBS"
FT   CDS             3988..4704
FT                   /label="sfGFP"
FT   terminator      4716..4759
FT                   /label="T1 terminator"
FT   terminator      4793..4833
FT                   /label="T7Te terminator"
FT   misc_feature    4838..5027
FT                   /label="ConRE' connector"
SQ   SEQUENCE  5027 AA; 1554058 MW;
     TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT        60
     GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA       120
     AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT       180
     CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC       240
     CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA       300
     TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT       360
     GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG       420
     CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC       480
     CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA       540
     AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA       600
     CGACAAGGTG TAAGGAGGAC TAAACCATGG GTAAGGAAAA GACTCACGTT TCGAGGCCGC       660
     GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG       720
     GGCAATCAGG TGCGACAATC TATCGATTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC       780
     TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT       840
     GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG       900
     CATGGTTACT CACCACTGCG ATCCCCGGCA AAACAGCATT CCAGGTATTA GAAGAATATC       960
     CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA      1020
     TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGCCTGGCT CAGGCGCAAT      1080
     CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC      1140
     CTGTTGAACA AGTCTGGAAA GAAATGCATA AGCTTTTGCC ATTCTCACCG GATTCAGTCG      1200
     TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT      1260
     GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA      1320
     ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG      1380
     ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGACGAGTTC TTCTAAGCCG      1440
     AGTAACTGAC TGACAATAAA AAGATTCTTG TTTTCAAGAA CTTGTCATTT GTATAGTTTT      1500
     TTTATATTGT AGTTGTTCTA TTTTAATCAA ATGTTAGCGT GATTTATATT TTTTTTCGCC      1560
     TCGACATCAT CTGCCCAGAT GCGAAGTTAA GTGCGCAGAA AGTAATATCA TGCGTCAATC      1620
     GTATGTGAAT GCTGGTCGCT ATACTGGAGT GTACTGAGAG TGCACCATAT GCGGTGTGAA      1680
     ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG      1740
     CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA CCGAGATTAT      1800
     CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA      1860
     GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT      1920
     CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA      1980
     CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGG GACCCACGCT      2040
     CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG      2100
     GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA      2160
     GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT      2220
     CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA      2280
     CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA      2340
     GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA      2400
     CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT      2460
     GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG      2520
     CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC      2580
     TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT      2640
     GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA      2700
     ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT      2760
     TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT      2820
     GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG      2880
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA      2940
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA      3000
     AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC      3060
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT      3120
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC      3180
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC      3240
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA      3300
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG      3360
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG      3420
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT      3480
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT      3540
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC      3600
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTGA ATTCGCATCT      3660
     AGACTGATGA GACGTGGTAG AGCCACAAAC AGCCGGTACA AGCAACGATC TCCAGGACCA      3720
     TCTGAATCAT GCGCGGATGA CACGAACTCA CGACGGCGAT CACAGACATT AACCCACAGT      3780
     ACAGACACTG CGACAACGTG GCAATTCGTC GCAATACAAC GGAAAGTGAA ACGTGATTTC      3840
     ATGCGTCATT TTGAACATTT TGTAAATCTT ATTTAATAAT GTGTGCGGCA ATTCACATTT      3900
     AATTTATGAA TGTTTTCTTA ACATCGCGGC AACTCAAGAA ACGGCAGGTT CGGATCTTAG      3960
     CTACTAGAGA AAGAGGAGAA ATACTAGATG CGTAAAGGCG AAGAGCTGTT CACTGGTGTC      4020
     GTCCCTATTC TGGTGGAACT GGATGGTGAT GTCAACGGTC ATAAGTTTTC CGTGCGTGGC      4080
     GAGGGTGAAG GTGACGCAAC TAATGGTAAA CTGACGCTGA AGTTCATCTG TACTACTGGT      4140
     AAACTGCCGG TTCCTTGGCC GACTCTGGTA ACGACGCTGA CTTATGGTGT TCAGTGCTTT      4200
     GCTCGTTATC CGGACCATAT GAAGCAGCAT GACTTCTTCA AGTCCGCCAT GCCGGAAGGC      4260
     TATGTGCAGG AACGCACGAT TTCCTTTAAG GATGACGGCA CGTACAAAAC GCGTGCGGAA      4320
     GTGAAATTTG AAGGCGATAC CCTGGTAAAC CGCATTGAGC TGAAAGGCAT TGACTTTAAA      4380
     GAGGACGGCA ATATCCTGGG CCATAAGCTG GAATACAATT TTAACAGCCA CAATGTTTAC      4440
     ATCACCGCCG ATAAACAAAA AAATGGCATT AAAGCGAATT TTAAAATTCG CCACAACGTG      4500
     GAGGATGGCA GCGTGCAGCT GGCTGATCAC TACCAGCAAA ACACTCCAAT CGGTGATGGT      4560
     CCTGTTCTGC TGCCAGACAA TCACTATCTG AGCACGCAAA GCGTTCTGTC TAAAGATCCG      4620
     AACGAGAAAC GCGATCATAT GGTTCTGCTG GAGTTCGTAA CCGCAGCGGG CATCACGCAT      4680
     GGTATGGATG AACTGTACAA ATGACCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG      4740
     ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCTACTAG AGTCACACTG      4800
     GCTCACCTTC GGGTGGGCCT TTCTGCGTTT ATAGCTGGAA ATCTGCTCGT CAGTGGTGCT      4860
     CACACTGACG AATCATGTAC AGATCATACC GATGACTGCC TGGCGACTCA CAACTAAGCA      4920
     AGACAGCCGG AACCAGCGCC GGCGAACACC ACTGCATATA TGGCATATCA CAACAGTCCA      4980
     CGTCTCAAGC AGTTACAGAG ATGTTACGAA CCACTAGTGC ACTGCAG                    5027
//
