ID   pPTK076_P8_Lox71-HygR-Lox66; SV 1; circular; unknown; STD; UNC; 3666 BP.
DT   16-OCT.-2023
DE   LMBP 12790.
CC   Accession number: LMBP 12790
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 16-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   rep_origin      447..764
FT                   /label="pMB1 ori"
FT   promoter        778..831
FT                   /label="PLtetO-1 promoter"
FT   RBS             832..856
FT                   /label="synthetic RBS"
FT   CDS             857..1534
FT                   /label="mRFP1"
FT   terminator      1555..1598
FT                   /label="T1 terminator"
FT   terminator      1632..1672
FT                   /label="T7Te terminator"
FT   misc_recomb     1713..1746
FT                   /label="Lox71"
FT   promoter        1747..2303
FT                   /label="ILV5 promoter"
FT   promoter        2304..2368
FT                   /label="synthetic EM72 promoter"
FT   CDS             2369..3397
FT                   /label="hygromycin resistance"
FT   terminator      3402..3607
FT                   /label="E. gossypii TEF1 terminator"
FT   misc_recomb     3608..3641
FT                   /label="Lox66"
SQ   SEQUENCE  3666 AA; 1133848 MW;
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA        60
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA       120
     AAAAACCACC GCTACCAGCG GTGGTTTTTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC       180
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT       240
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC       300
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC       360
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA       420
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG       480
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG       540
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT       600
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT       660
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC       720
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC       780
     CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA       840
     AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA       900
     AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG       960
     TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC      1020
     GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA      1080
     CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG      1140
     TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA      1200
     CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT      1260
     TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG      1320
     TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC      1380
     TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC      1440
     CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA      1500
     ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA      1560
     CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT      1620
     CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC      1680
     TCACCGAACA GGGACTAAAG AGGTGATGAT TATACCGTTC GTATAGCATA CATTATACGA      1740
     AGTTATATCC TTCAGTAATG TCTTGTTTCT TTTGTTGCAG TGGTGAGCCA TTTTGACTTC      1800
     GTGAAAGTTT CTTTAGAATA GTTGTTTCCA GAGGCCAAAC ATTCCACCCG TAGTAAAGTG      1860
     CAAGCGTAGG AGAACCAAGA CTGGCATAAA TCAGGTATAA GTGTCGAGCA CTGGCAGGTG      1920
     ATCTTCTGAA AGTTTCTACT AGCAGATAAG ATCCAGTAGT CATGCATATG GCAACAATGT      1980
     ACCGTGTGGA TCTAAGAACG CGTCCTACTA ACCTTCGCAT TCGTTGGTCC AGTTTGTTGT      2040
     TATCGATCAA CGTGACAAGG TTGTCGATTC CGCGTAAGCA TGCATACCCA AGGACGCCTG      2100
     TTGCAATTCC AAGTGAGCCA GTTCCAACAA TCTTTGTAAT ATTAGAGCAC TTCATTGTGT      2160
     TGCGCTTGAA AGTAAAATGC GAACAAATTA AGAGATAATC TCGAAACCGC GACTTCAAAC      2220
     GCCAATATGA TGTGCGGCAC ACAATAAGCG TTCATATCCG CTGGGTGACT TTCTCGCTTT      2280
     AAAAAATTAT CCGAAAAAAT TTTCTAGAGT GTTGTTACTT TATACTTCCG GCTCGTATAA      2340
     TACGACAAGG TGTAAGGAGG ACTAAACCAT GGGTAAAAAG CCTGAACTCA CCGCGACGTC      2400
     TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTGTCC GACCTGATGC AGCTCTCGGA      2460
     GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT      2520
     AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC      2580
     CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTT AGCGAGAGCC TGACCTATTG      2640
     CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC      2700
     TGTTCTGCAA CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC      2760
     GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT      2820
     CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT      2880
     CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA      2940
     AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG      3000
     CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC      3060
     CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA      3120
     GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG      3180
     TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA      3240
     GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC      3300
     CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA      3360
     CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAAGCC GAGTAACTGA CTGACAATAA      3420
     AAAGATTCTT GTTTTCAAGA ACTTGTCATT TGTATAGTTT TTTTATATTG TAGTTGTTCT      3480
     ATTTTAATCA AATGTTAGCG TGATTTATAT TTTTTTTCGC CTCGACATCA TCTGCCCAGA      3540
     TGCGAAGTTA AGTGCGCAGA AAGTAATATC ATGCGTCAAT CGTATGTGAA TGCTGGTCGC      3600
     TATACTGATA ACTTCGTATA GCATACATTA TACGAACGGT AGACAGTATC GAATGGACTC      3660
     TGATGA                                                                 3666
//
