ID   pMC_MultiGene_BlastR; SV 1; circular; unknown; STD; UNC; 4608 BP.
DT   17-OCT.-2023
DE   LMBP 12798.
CC   Accession number: LMBP 12798
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 17-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        5..561
FT                   /label="ILV5 promoter"
FT   promoter        562..626
FT                   /label="synthetic EM72 promoter"
FT   CDS             627..1025
FT                   /label="blasticidin resistance"
FT   terminator      1026..1227
FT                   /label="E. gossypii TEF1 terminator"
FT   CDS             complement(1470..2330)
FT                   /label="ampicillin resistance"
FT   rep_origin      2908..3225
FT                   /label="pMB1 ori"
FT   misc_feature    3230..3398
FT                   /label="ConLS' connector"
FT   promoter        3403..3532
FT                   /label="glpT promoter"
FT   RBS             3533..3568
FT                   /label="synthetic RBS"
FT   CDS             3569..4285
FT                   /label="sfGFP"
FT   terminator      4297..4340
FT                   /label="T1 terminator"
FT   terminator      4374..4414
FT                   /label="T7Te terminator"
FT   misc_feature    4419..4608
FT                   /label="ConRE' connector"
SQ   SEQUENCE  4608 AA; 1424267 MW;
     TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT        60
     GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA       120
     AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT       180
     CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC       240
     CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA       300
     TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT       360
     GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG       420
     CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC       480
     CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA       540
     AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA       600
     CGACAAGGTG TAAGGAGGAC TAAACCATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC       660
     TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAT TACAGCGTCG       720
     CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA       780
     CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA       840
     ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC       900
     GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG       960
     ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG      1020
     GCTAAAGTAA CTGACAATAA AAAGATTCTT GTTTTCAAGA ACTTGTCATT TGTATAGTTT      1080
     TTTTATATTG TAGTTGTTCT ATTTTAATCA AATGTTAGCG TGATTTATAT TTTTTTTCGC      1140
     CTCGACATCA TCTGCCCAGA TGCGAAGTTA AGTGCGCAGA AAGTAATATC ATGCGTCAAT      1200
     CGTATGTGAA TGCTGGTCGC TATACTGGAG TGTACTGAGA GTGCACCATA TGCGGTGTGA      1260
     AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT      1320
     GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC ACCGAGATTA      1380
     TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA      1440
     AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC      1500
     TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT      1560
     ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG GGACCCACGC      1620
     TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT      1680
     GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA      1740
     AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG      1800
     TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT      1860
     ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC      1920
     AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT      1980
     ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC      2040
     TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC      2100
     GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA      2160
     CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC      2220
     TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA      2280
     AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT      2340
     TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA      2400
     TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT      2460
     GTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA      2520
     AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA      2580
     AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT      2640
     CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG      2700
     TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC      2760
     CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA      2820
     CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC      2880
     AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC      2940
     GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA      3000
     GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG      3060
     TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA      3120
     TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT      3180
     CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTCCCTG AATTCGCATC      3240
     TAGACTGATG AGACGTGGTA GAGCCACAAA CAGCCGGTAC AAGCAACGAT CTCCAGGACC      3300
     ATCTGAATCA TGCGCGGATG ACACGAACTC ACGACGGCGA TCACAGACAT TAACCCACAG      3360
     TACAGACACT GCGACAACGT GGCAATTCGT CGCAATACAA CGGAAAGTGA AACGTGATTT      3420
     CATGCGTCAT TTTGAACATT TTGTAAATCT TATTTAATAA TGTGTGCGGC AATTCACATT      3480
     TAATTTATGA ATGTTTTCTT AACATCGCGG CAACTCAAGA AACGGCAGGT TCGGATCTTA      3540
     GCTACTAGAG AAAGAGGAGA AATACTAGAT GCGTAAAGGC GAAGAGCTGT TCACTGGTGT      3600
     CGTCCCTATT CTGGTGGAAC TGGATGGTGA TGTCAACGGT CATAAGTTTT CCGTGCGTGG      3660
     CGAGGGTGAA GGTGACGCAA CTAATGGTAA ACTGACGCTG AAGTTCATCT GTACTACTGG      3720
     TAAACTGCCG GTTCCTTGGC CGACTCTGGT AACGACGCTG ACTTATGGTG TTCAGTGCTT      3780
     TGCTCGTTAT CCGGACCATA TGAAGCAGCA TGACTTCTTC AAGTCCGCCA TGCCGGAAGG      3840
     CTATGTGCAG GAACGCACGA TTTCCTTTAA GGATGACGGC ACGTACAAAA CGCGTGCGGA      3900
     AGTGAAATTT GAAGGCGATA CCCTGGTAAA CCGCATTGAG CTGAAAGGCA TTGACTTTAA      3960
     AGAGGACGGC AATATCCTGG GCCATAAGCT GGAATACAAT TTTAACAGCC ACAATGTTTA      4020
     CATCACCGCC GATAAACAAA AAAATGGCAT TAAAGCGAAT TTTAAAATTC GCCACAACGT      4080
     GGAGGATGGC AGCGTGCAGC TGGCTGATCA CTACCAGCAA AACACTCCAA TCGGTGATGG      4140
     TCCTGTTCTG CTGCCAGACA ATCACTATCT GAGCACGCAA AGCGTTCTGT CTAAAGATCC      4200
     GAACGAGAAA CGCGATCATA TGGTTCTGCT GGAGTTCGTA ACCGCAGCGG GCATCACGCA      4260
     TGGTATGGAT GAACTGTACA AATGACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA      4320
     GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT      4380
     GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATAGCTGGA AATCTGCTCG TCAGTGGTGC      4440
     TCACACTGAC GAATCATGTA CAGATCATAC CGATGACTGC CTGGCGACTC ACAACTAAGC      4500
     AAGACAGCCG GAACCAGCGC CGGCGAACAC CACTGCATAT ATGGCATATC ACAACAGTCC      4560
     ACGTCTCAAG CAGTTACAGA GATGTTACGA ACCACTAGTG CACTGCAG                   4608
//
