ID   pMC_MultiGene_HygR; SV 1; circular; unknown; STD; UNC; 5246 BP.
DT   17-OCT.-2023
DE   LMBP 12796.
CC   Accession number: LMBP 12796
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 17-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        5..561
FT                   /label="ILV5 promoter"
FT   promoter        562..626
FT                   /label="synthetic EM72 promoter"
FT   CDS             627..1655
FT                   /label="hygromycin resistance"
FT   terminator      1660..1865
FT                   /label="E. gossypii TEF1 terminator"
FT   CDS             complement(2108..2968)
FT                   /label="ampicillin resistance"
FT   rep_origin      3546..3863
FT                   /label="pMB1 ori"
FT   misc_feature    3868..4036
FT                   /label="ConLS' connector"
FT   promoter        4041..4170
FT                   /label="glpT promoter"
FT   RBS             4171..4206
FT                   /label="synthetic RBS"
FT   CDS             4207..4923
FT                   /label="sfGFP"
FT   terminator      4935..4978
FT                   /label="T1 terminator"
FT   terminator      5012..5052
FT                   /label="T7Te terminator"
FT   misc_feature    5057..5246
FT                   /label="ConRE' connector"
SQ   SEQUENCE  5246 AA; 1621885 MW;
     TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT        60
     GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA       120
     AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT       180
     CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC       240
     CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA       300
     TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT       360
     GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG       420
     CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC       480
     CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA       540
     AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA       600
     CGACAAGGTG TAAGGAGGAC TAAACCATGG GTAAAAAGCC TGAACTCACC GCGACGTCTG       660
     TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTGTCCGA CCTGATGCAG CTCTCGGAGG       720
     GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA       780
     ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG       840
     CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTTAG CGAGAGCCTG ACCTATTGCA       900
     TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG       960
     TTCTGCAACC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA      1020
     GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA      1080
     TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA      1140
     GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG      1200
     TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA      1260
     TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA      1320
     ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC      1380
     GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC CGCATTGGTC      1440
     TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG      1500
     GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC      1560
     GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC      1620
     GACGCCCCAG CACTCGTCCG AGGGCAAAGG AATAAGCCGA GTAACTGACT GACAATAAAA      1680
     AGATTCTTGT TTTCAAGAAC TTGTCATTTG TATAGTTTTT TTATATTGTA GTTGTTCTAT      1740
     TTTAATCAAA TGTTAGCGTG ATTTATATTT TTTTTCGCCT CGACATCATC TGCCCAGATG      1800
     CGAAGTTAAG TGCGCAGAAA GTAATATCAT GCGTCAATCG TATGTGAATG CTGGTCGCTA      1860
     TACTGGAGTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG      1920
     AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA      1980
     TCGGTGCGGG CCTCTTCGCT ATTACGCCAC CGAGATTATC AAAAAGGATC TTCACCTAGA      2040
     TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT      2100
     CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT      2160
     CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT      2220
     CTGGCCCCAG TGCTGCAATG ATACCGCGGG ACCCACGCTC ACCGGCTCCA GATTTATCAG      2280
     CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT      2340
     CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT      2400
     TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG      2460
     CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA      2520
     AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT      2580
     TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT      2640
     GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC      2700
     CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA      2760
     AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT      2820
     TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT      2880
     TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA      2940
     GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT      3000
     ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA      3060
     TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGT CATGACCAAA ATCCCTTAAC      3120
     GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG      3180
     ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG      3240
     TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA      3300
     GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA      3360
     ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA      3420
     GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC      3480
     AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA      3540
     CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA      3600
     AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC      3660
     CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC      3720
     GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG      3780
     CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT      3840
     CCCCTGATTC TGTGGATAAC CGTCCCTGAA TTCGCATCTA GACTGATGAG ACGTGGTAGA      3900
     GCCACAAACA GCCGGTACAA GCAACGATCT CCAGGACCAT CTGAATCATG CGCGGATGAC      3960
     ACGAACTCAC GACGGCGATC ACAGACATTA ACCCACAGTA CAGACACTGC GACAACGTGG      4020
     CAATTCGTCG CAATACAACG GAAAGTGAAA CGTGATTTCA TGCGTCATTT TGAACATTTT      4080
     GTAAATCTTA TTTAATAATG TGTGCGGCAA TTCACATTTA ATTTATGAAT GTTTTCTTAA      4140
     CATCGCGGCA ACTCAAGAAA CGGCAGGTTC GGATCTTAGC TACTAGAGAA AGAGGAGAAA      4200
     TACTAGATGC GTAAAGGCGA AGAGCTGTTC ACTGGTGTCG TCCCTATTCT GGTGGAACTG      4260
     GATGGTGATG TCAACGGTCA TAAGTTTTCC GTGCGTGGCG AGGGTGAAGG TGACGCAACT      4320
     AATGGTAAAC TGACGCTGAA GTTCATCTGT ACTACTGGTA AACTGCCGGT TCCTTGGCCG      4380
     ACTCTGGTAA CGACGCTGAC TTATGGTGTT CAGTGCTTTG CTCGTTATCC GGACCATATG      4440
     AAGCAGCATG ACTTCTTCAA GTCCGCCATG CCGGAAGGCT ATGTGCAGGA ACGCACGATT      4500
     TCCTTTAAGG ATGACGGCAC GTACAAAACG CGTGCGGAAG TGAAATTTGA AGGCGATACC      4560
     CTGGTAAACC GCATTGAGCT GAAAGGCATT GACTTTAAAG AGGACGGCAA TATCCTGGGC      4620
     CATAAGCTGG AATACAATTT TAACAGCCAC AATGTTTACA TCACCGCCGA TAAACAAAAA      4680
     AATGGCATTA AAGCGAATTT TAAAATTCGC CACAACGTGG AGGATGGCAG CGTGCAGCTG      4740
     GCTGATCACT ACCAGCAAAA CACTCCAATC GGTGATGGTC CTGTTCTGCT GCCAGACAAT      4800
     CACTATCTGA GCACGCAAAG CGTTCTGTCT AAAGATCCGA ACGAGAAACG CGATCATATG      4860
     GTTCTGCTGG AGTTCGTAAC CGCAGCGGGC ATCACGCATG GTATGGATGA ACTGTACAAA      4920
     TGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT      4980
     GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT      5040
     TCTGCGTTTA TAGCTGGAAA TCTGCTCGTC AGTGGTGCTC ACACTGACGA ATCATGTACA      5100
     GATCATACCG ATGACTGCCT GGCGACTCAC AACTAAGCAA GACAGCCGGA ACCAGCGCCG      5160
     GCGAACACCA CTGCATATAT GGCATATCAC AACAGTCCAC GTCTCAAGCA GTTACAGAGA      5220
     TGTTACGAAC CACTAGTGCA CTGCAG                                           5246
//
