ID   pPTK080_P8_AmpR; SV 1; circular; unknown; STD; UNC; 2773 BP.
DT   23-OCT-2023
DE   LMBP 12785.
CC   Accession number: LMBP 12785
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   rep_origin      447..764
FT                   /label="pMB1 ori"
FT   promoter        778..831
FT                   /label="PLtetO-1 promoter"
FT   RBS             832..856
FT                   /label="synthetic RBS"
FT   CDS             857..1534
FT                   /label="mRFP1"
FT   terminator      1555..1598
FT                   /label="T1 terminator"
FT   terminator      1632..1672
FT                   /label="T7Te terminator"
FT   CDS             complement(1782..2642)
FT                   /label="ampicillin resistance"
SQ   SEQUENCE  2773 AA;  856541 MW;
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA        60
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA       120
     AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC       180
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT       240
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC       300
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC       360
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA       420
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG       480
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG       540
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT       600
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT       660
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC       720
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC       780
     CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA       840
     AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA       900
     AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG       960
     TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC      1020
     GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA      1080
     CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG      1140
     TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA      1200
     CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT      1260
     TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG      1320
     TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC      1380
     TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC      1440
     CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA      1500
     ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA      1560
     CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT      1620
     CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC      1680
     TCACCGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT      1740
     AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT      1800
     GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC      1860
     GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG      1920
     CGGGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC      1980
     GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG      2040
     GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA      2100
     GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA      2160
     TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT      2220
     CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG      2280
     CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA      2340
     ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA      2400
     CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT      2460
     TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT      2520
     CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA      2580
     ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC      2640
     ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA      2700
     TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA      2760
     AAAGTGCCAC CTG                                                         2773
//
