ID   pPTK205_P6_Lox71-NourR-Lox66; SV 1; circular; unknown; STD; UNC; 3139 BP.
DT   23-OCT-2023
DE   LMBP 12774.
CC   Accession number: LMBP 12774
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(112..771)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      1323..1641
FT                   /label="pMB1 ori"
FT   misc_recomb     1656..1689
FT                   /label="Lox71"
FT   promoter        1690..2246
FT                   /label="ILV5 promoter"
FT   promoter        2247..2311
FT                   /label="synthetic EM72 promoter"
FT   CDS             2312..2884
FT                   /label="nourseothricin resistance"
FT   terminator      2897..3094
FT                   /label="E. gossypii TEF1 terminator"
FT   misc_recomb     3095..3128
FT                   /label="Lox66"
SQ   SEQUENCE  3139 AA;  970438 MW;
     AGACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT        60
     GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC       120
     GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC       180
     ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT       240
     ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA       300
     ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAAACG AAAAACATAT TCTCAATAAA       360
     CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG       420
     TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG       480
     CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT       540
     CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC       600
     CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG       660
     AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG       720
     ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC       780
     CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT       840
     ATGGTGAAAG TTGGAACCTC TTACGTGCCC GATCAATCAT GACCAAAATC CCTTAACGTG       900
     AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC       960
     CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG      1020
     TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG      1080
     CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT      1140
     CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG      1200
     GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC      1260
     GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG      1320
     AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG      1380
     CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG      1440
     GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC      1500
     GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT      1560
     TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC      1620
     CTGATTCTGT GGATAACCGT AGTCGGTCTC ATACATACCG TTCGTATAGC ATACATTATA      1680
     CGAAGTTATA TCCTTCAGTA ATGTCTTGTT TCTTTTGTTG CAGTGGTGAG CCATTTTGAC      1740
     TTCGTGAAAG TTTCTTTAGA ATAGTTGTTT CCAGAGGCCA AACATTCCAC CCGTAGTAAA      1800
     GTGCAAGCGT AGGAGAACCA AGACTGGCAT AAATCAGGTA TAAGTGTCGA GCACTGGCAG      1860
     GTGATCTTCT GAAAGTTTCT ACTAGCAGAT AAGATCCAGT AGTCATGCAT ATGGCAACAA      1920
     TGTACCGTGT GGATCTAAGA ACGCGTCCTA CTAACCTTCG CATTCGTTGG TCCAGTTTGT      1980
     TGTTATCGAT CAACGTGACA AGGTTGTCGA TTCCGCGTAA GCATGCATAC CCAAGGACGC      2040
     CTGTTGCAAT TCCAAGTGAG CCAGTTCCAA CAATCTTTGT AATATTAGAG CACTTCATTG      2100
     TGTTGCGCTT GAAAGTAAAA TGCGAACAAA TTAAGAGATA ATCTCGAAAC CGCGACTTCA      2160
     AACGCCAATA TGATGTGCGG CACACAATAA GCGTTCATAT CCGCTGGGTG ACTTTCTCGC      2220
     TTTAAAAAAT TATCCGAAAA AATTTTCTAG AGTGTTGTTA CTTTATACTT CCGGCTCGTA      2280
     TAATACGACA AGGTGTAAGG AGGACTAAAC CATGGGTACT ACCTTAGATG ATACAGCCTA      2340
     CAGATACAGA ACATCAGTCC CTGGTGATGC TGAAGCAATT GAGGCTTTAG ACGGTTCATT      2400
     CACCACCGAC ACCGTCTTTA GAGTAACCGC CACCGGTGAT GGATTTACCT TAAGAGAAGT      2460
     CCCAGTCGAC CCTCCATTAA CTAAAGTCTT TCCAGATGAT GAATCTGATG ACGAAAGCGA      2520
     CGACGGAGAA GATGGTGACC CAGATTCAAG AACTTTCGTA GCATACGGTG ATGACGGTGA      2580
     TTTGGCTGGT TTTGTAGTCG TTTCTTATTC AGGTTGGAAT AGAAGGTTGA CCGTTGAAGA      2640
     TATAGAAGTC GCCCCAGAGC ATAGAGGTCA TGGTGTAGGA AGAGCTTTGA TGGGTTTGGC      2700
     TACAGAATTT GCAAGAGAGA GAGGAGCCGG TCATTTATGG TTAGAAGTTA CTAATGTTAA      2760
     CGCCCCTGCT ATCCATGCTT ATAGAAGAAT GGGTTTCACA TTATGTGGTT TAGATACTGC      2820
     TTTATATGAT GGAACAGCAT CTGACGGTGA ACAGGCCTTG TATATGTCTA TGCCTTGCCC      2880
     TTAAGCCGAG TAACTGACTG ACAATAAAAA GATTCTTGTT TTCAAGAACT TGTCATTTGT      2940
     ATAGTTTTTT TATATTGTAG TTGTTCTATT TTAATCAAAT GTTAGCGTGA TTTATATTTT      3000
     TTTTCGCCTC GACATCATCT GCCCAGATGC GAAGTTAAGT GCGCAGAAAG TAATATCATG      3060
     CGTCAATCGT ATGTGAATGC TGGTCGCTAT ACTGATAACT TCGTATAGCA TACATTATAC      3120
     GAACGGTAGA GTTGAGACC                                                   3139
//
