ID   pPTK200_P6_HygR; SV 1; circular; unknown; STD; UNC; 3527 BP.
DT   23-OCT-2023
DE   LMBP 12768.
CC   Accession number: LMBP 12768
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(112..771)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      1323..1641
FT                   /label="pMB1 ori"
FT   promoter        1656..2212
FT                   /label="ILV5 promoter"
FT   promoter        2213..2277
FT                   /label="synthetic EM72 promoter"
FT   CDS             2278..3306
FT                   /label="hygromycin resistance"
FT   terminator      3319..3516
FT                   /label="E. gossypii TEF1 terminator"
SQ   SEQUENCE  3527 AA; 1090334 MW;
     AGACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT        60
     GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC       120
     GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC       180
     ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT       240
     ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA       300
     ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAAACG AAAAACATAT TCTCAATAAA       360
     CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG       420
     TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG       480
     CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT       540
     CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC       600
     CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG       660
     AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG       720
     ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC       780
     CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT       840
     ATGGTGAAAG TTGGAACCTC TTACGTGCCC GATCAATCAT GACCAAAATC CCTTAACGTG       900
     AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC       960
     CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG      1020
     TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG      1080
     CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT      1140
     CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG      1200
     GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC      1260
     GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG      1320
     AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG      1380
     CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG      1440
     GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC      1500
     GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT      1560
     TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC      1620
     CTGATTCTGT GGATAACCGT AGTCGGTCTC ATACAATCCT TCAGTAATGT CTTGTTTCTT      1680
     TTGTTGCAGT GGTGAGCCAT TTTGACTTCG TGAAAGTTTC TTTAGAATAG TTGTTTCCAG      1740
     AGGCCAAACA TTCCACCCGT AGTAAAGTGC AAGCGTAGGA GAACCAAGAC TGGCATAAAT      1800
     CAGGTATAAG TGTCGAGCAC TGGCAGGTGA TCTTCTGAAA GTTTCTACTA GCAGATAAGA      1860
     TCCAGTAGTC ATGCATATGG CAACAATGTA CCGTGTGGAT CTAAGAACGC GTCCTACTAA      1920
     CCTTCGCATT CGTTGGTCCA GTTTGTTGTT ATCGATCAAC GTGACAAGGT TGTCGATTCC      1980
     GCGTAAGCAT GCATACCCAA GGACGCCTGT TGCAATTCCA AGTGAGCCAG TTCCAACAAT      2040
     CTTTGTAATA TTAGAGCACT TCATTGTGTT GCGCTTGAAA GTAAAATGCG AACAAATTAA      2100
     GAGATAATCT CGAAACCGCG ACTTCAAACG CCAATATGAT GTGCGGCACA CAATAAGCGT      2160
     TCATATCCGC TGGGTGACTT TCTCGCTTTA AAAAATTATC CGAAAAAATT TTCTAGAGTG      2220
     TTGTTACTTT ATACTTCCGG CTCGTATAAT ACGACAAGGT GTAAGGAGGA CTAAACCATG      2280
     GGTAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC      2340
     AGCGTGTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT      2400
     GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT      2460
     CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT      2520
     GGGGAATTTA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG      2580
     CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAAC CGGTCGCGGA GGCCATGGAT      2640
     GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA      2700
     ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT      2760
     CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG      2820
     CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC      2880
     TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG      2940
     ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT      3000
     TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG      3060
     CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC      3120
     GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA      3180
     GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC      3240
     TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG      3300
     GAATAAGCCG AGTAACTGAC TGACAATAAA AAGATTCTTG TTTTCAAGAA CTTGTCATTT      3360
     GTATAGTTTT TTTATATTGT AGTTGTTCTA TTTTAATCAA ATGTTAGCGT GATTTATATT      3420
     TTTTTTCGCC TCGACATCAT CTGCCCAGAT GCGAAGTTAA GTGCGCAGAA AGTAATATCA      3480
     TGCGTCAATC GTATGTGAAT GCTGGTCGCT ATACTGGAGT TGAGACC                    3527
//
