ID   pMC_Cre_HygR; SV 1; circular; unknown; STD; UNC; 6552 BP.
DT   09-OCT-2023
DE   LMBP 1280.
CC   Accession number: LMBP 12801
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 9-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(95..955)
FT                   /label="ampicillin resistance"
FT   rep_origin      1533..1850
FT                   /label="pMB1 ori"
FT   misc_feature    1855..2043
FT                   /label="ConLS connector"
FT   promoter        join(2048..3045)
FT                   /label="S. cerevisiae ENO1 promoter"
FT   CDS             3052..4086
FT                   /label="Cre"
FT   terminator      4087..4330
FT                   /label="AOX1 terminator"
FT   misc_feature    4335..4503
FT                   /label="ConR1 connector"
FT   promoter        4508..5064
FT                   /label="ILV5 promoter"
FT   promoter        5065..5129
FT                   /label="synthetic EM72 promoter"
FT   CDS             5130..6158
FT                   /label="hygromycin resistance"
FT   terminator      6171..6368
FT                   /label="E. gossypii TEF1 terminator"
FT   rep_origin      6379..6542
FT                   /label="Pichia ARS1"
SQ   SEQUENCE  6552 AA; 2024585 MW;
     GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA        60
     TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC       120
     CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA       180
     TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGGGACC       240
     CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA       300
     GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA       360
     GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG       420
     TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC       480
     GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG       540
     TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT       600
     CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT       660
     CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA       720
     ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC       780
     GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC       840
     CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA       900
     GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT       960
     TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT      1020
     TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC      1080
     CACCTGTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG      1140
     TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC      1200
     AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC      1260
     TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT      1320
     AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC      1380
     TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT      1440
     CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC      1500
     AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG      1560
     AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG      1620
     GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG      1680
     TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA      1740
     GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT      1800
     TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT CCCTAATTCG      1860
     CATCTAGATG GTAGAGCCAC AAACAGCCGG TACAAGCAAC GATCTCCAGG ACCATCTGAA      1920
     TCATGCGCGG ATGACACGAA CTCACGACGG CGATCACAGA CATTAACCCA CAGTACAGAC      1980
     ACTGCGACAA CGTGGCAATT CGTCGCAATA CCGTCTCACT GAACTGGCCG ATAATTGCAG      2040
     ACGAACGAGA AAGCATACTA TACTATTCGA CATTCCTTTC AATCCTGGAA TTAACAGTCA      2100
     CTTTTAAAAA AGACATCTAC CGTGAAGGTG CCGTAGAGTA TCGCGTTACC ATATCGCCAA      2160
     AAACTGATAT ACGCCGCGGA AACCAGGCAA ACAATTGAAA AGAAAAATTT TGAGGAACTC      2220
     TCTGCATCGA AGCCGTCTAG AGTTACCACT AGTCAGATGC CGCGGGCACT TGAGCACCTC      2280
     ATGCACAGCA ATAACACAAC ACAATGGTTA GTAGCAACCT GAATTCGGTC ATTGATGCAT      2340
     GCATGTGCCG TGAAGCGGGA CAACCAGAAA AGTCGTCTAT AAATGCCGGC ACGTGCGATC      2400
     ATCGTGGCGG GGTTTTAAGA GTGCATATCA CAAATTGTCG CATTACCGCG GAACCGCCAG      2460
     ATATTCATTA CTTGACGCAA AAGCGTTTGA AATAATGACG AAAAAGAAGG AAGAAAAAAA      2520
     AAGAAAAATA CCGCTTCTAG GCGGGTTATC TACTGATCCG AGCTTCCACT AGGATAGCAC      2580
     CCAAACACCT GCATATTTGG ACGACCTTTA CTTACACCAC CAAAAACCAC TTTCGCCTCT      2640
     CCCGCCCCTG ATAACGTCCA CTAATTGAGC GATTACCTGA GCGGTCCTCT TTTGTTTGCA      2700
     GCATGAGACT TGCATACTGC AAATCGTAAG TAGCAACCTC TCAAGGTCAA AACTGTATGG      2760
     AAACCTTGTC ACCTCACTTA ATTCTAGCTA GCCTACCCTG CAAGTCAAGA GCTCTCCGTG      2820
     ATTCCTAGCC ACCTCAAGGT ATGCCTCTCC CCGGAAACTG TGGCCTTTTC TGGCACACAT      2880
     GATCTCCACG ATTTCAACAT ATAAATAGCT TTTGATAATG GCAATATTAA TCAAATTTAT      2940
     TTTACTTCTT TCTTGTAACA TCTCTCTTGT AATCCCTTAT TCCTTCTAGC TATTTTTCAT      3000
     AAAAAACCAA GCAACTGCTT ATCAACACAC AAACACTAAA TCAAAAGATC TATGTCCAAT      3060
     TTACTGACCG TACACCAAAA TTTGCCTGCA TTACCGGTCG ATGCAACGAG TGATGAGGTT      3120
     CGCAAGAACC TGATGGACAT GTTCAGGGAT CGCCAGGCGT TTTCTGAGCA TACCTGGAAA      3180
     ATGCTTCTGT CCGTTTGCCG GTCGTGGGCG GCATGGTGCA AGTTGAATAA CCGGAAATGG      3240
     TTTCCCGCAG AACCTGAAGA TGTTCGCGAT TATCTTCTAT ATCTTCAGGC GCGCGGTCTG      3300
     GCAGTAAAAA CTATCCAGCA ACATTTGGGC CAGCTAAACA TGCTTCATCG TCGGTCCGGG      3360
     CTGCCACGAC CAAGTGACAG CAATGCTGTT TCACTGGTTA TGCGGCGGAT CCGAAAAGAA      3420
     AACGTTGATG CCGGTGAACG TGCAAAACAG GCTCTAGCGT TCGAACGCAC TGATTTCGAC      3480
     CAGGTTCGTT CACTCATGGA AAATAGCGAT CGCTGCCAGG ATATACGTAA TCTGGCATTT      3540
     CTGGGGATTG CTTATAACAC CCTGTTACGT ATAGCCGAAA TTGCCAGGAT CAGGGTTAAA      3600
     GATATCTCAC GTACTGACGG TGGGAGAATG TTAATCCATA TTGGCAGAAC GAAAACGCTG      3660
     GTTAGCACCG CAGGTGTAGA GAAGGCACTT AGCCTGGGGG TAACTAAACT GGTCGAGCGA      3720
     TGGATTTCCG TGTCTGGTGT AGCTGATGAT CCGAATAACT ACCTGTTTTG CCGGGTCAGA      3780
     AAAAATGGTG TTGCCGCGCC ATCTGCCACC AGCCAGCTAT CAACTCGCGC CCTGGAAGGG      3840
     ATTTTTGAAG CAACTCATCG ATTGATTTAC GGCGCTAAGG ATGACTCTGG TCAGAGATAC      3900
     CTGGCCTGGT CTGGACACAG TGCCCGTGTC GGAGCCGCGC GAGATATGGC CCGCGCTGGA      3960
     GTTTCAATAC CGGAGATCAT GCAAGCTGGT GGCTGGACCA ATGTAAATAT TGTCATGAAC      4020
     TATATCCGTA ACCTGGATAG TGAAACAGGG GCAATGGTGC GCCTGCTGGA AGATGGCGGA      4080
     TCCTAATCAA GAGGATGTCA GAATGCCATT TGCCTGAGAG ATGCAGGCTT CATTTTTGAT      4140
     ACTTTTTTAT TTGTAACCTA TATAGTATAG GATTTTTTTT GTCATTTTGT TTCTTCTCGT      4200
     ACGAGCTTGC TCCTGATCAG CCTATCTCGC AGCTGATGAA TATCTTGTGG TAGGGGTTTG      4260
     GGAAAATCAT TCGAGTTTGA TGTTTTTCTT GGTATTTCCC ACTCCTCTTC AGAGTACAGA      4320
     AGATTAAGTG GCTGCCAATG AGACGACGGG GTCATCACGG CTCATCATGC GCCAAACAAA      4380
     TGTGTGCAAT ACACGCTCGG ATGACTGCAT GATGACCGCA CTGACTGGGG ACAGCAGATC      4440
     CACCTAAGCC TGTGAGAGAA GCAGACACCC GACAGATCAA GGCAGTTAAC TAGTGCACTG      4500
     CAGTACAATC CTTCAGTAAT GTCTTGTTTC TTTTGTTGCA GTGGTGAGCC ATTTTGACTT      4560
     CGTGAAAGTT TCTTTAGAAT AGTTGTTTCC AGAGGCCAAA CATTCCACCC GTAGTAAAGT      4620
     GCAAGCGTAG GAGAACCAAG ACTGGCATAA ATCAGGTATA AGTGTCGAGC ACTGGCAGGT      4680
     GATCTTCTGA AAGTTTCTAC TAGCAGATAA GATCCAGTAG TCATGCATAT GGCAACAATG      4740
     TACCGTGTGG ATCTAAGAAC GCGTCCTACT AACCTTCGCA TTCGTTGGTC CAGTTTGTTG      4800
     TTATCGATCA ACGTGACAAG GTTGTCGATT CCGCGTAAGC ATGCATACCC AAGGACGCCT      4860
     GTTGCAATTC CAAGTGAGCC AGTTCCAACA ATCTTTGTAA TATTAGAGCA CTTCATTGTG      4920
     TTGCGCTTGA AAGTAAAATG CGAACAAATT AAGAGATAAT CTCGAAACCG CGACTTCAAA      4980
     CGCCAATATG ATGTGCGGCA CACAATAAGC GTTCATATCC GCTGGGTGAC TTTCTCGCTT      5040
     TAAAAAATTA TCCGAAAAAA TTTTCTAGAG TGTTGTTACT TTATACTTCC GGCTCGTATA      5100
     ATACGACAAG GTGTAAGGAG GACTAAACCA TGGGTAAAAA GCCTGAACTC ACCGCGACGT      5160
     CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTGTC CGACCTGATG CAGCTCTCGG      5220
     AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT GTCCTGCGGG      5280
     TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC TTTGCATCGG      5340
     CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT TAGCGAGAGC CTGACCTATT      5400
     GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG      5460
     CTGTTCTGCA ACCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA      5520
     CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA TGGCGTGATT      5580
     TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG GACGACACCG      5640
     TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG GACTGCCCCG      5700
     AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC      5760
     GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG      5820
     CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG CGCTACTTCG      5880
     AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG      5940
     GTCTTGACCA ACTCTATCAG AGCTTGGTTG ACGGCAATTT CGATGATGCA GCTTGGGCGC      6000
     AGGGTCGATG CGACGCAATC GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG      6060
     CCCGCAGAAG CGCGGCCGTC TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA      6120
     ACCGACGCCC CAGCACTCGT CCGAGGGCAA AGGAATAAGC CGAGTAACTG ACTGACAATA      6180
     AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT TTTTTATATT GTAGTTGTTC      6240
     TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG CCTCGACATC ATCTGCCCAG      6300
     ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA TCGTATGTGA ATGCTGGTCG      6360
     CTATACTGGA GTATGCATTC GAGATAAGCT GGGGGAACAT TCGCGAAAAT GAAACAAGTC      6420
     GGCTGTTATA GTATATTTAT TATAATATTG AAAGATCTCA AAAGACTACT TATTTTTGAA      6480
     TGAACCAAGT ATGAAATCAA CCTATTTGGG GTTGACCAAA ATAAGTAAAT ATTAATTGTC      6540
     GAATGCATCC GA                                                          6552
//
