ID   pMC_Cre_KanR; SV 1; circular; unknown; STD; UNC; 6334 BP.
DT   17-OCT.-2023
DE   LMBP 12800.
CC   Accession number: LMBP 12800
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 17-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(95..955)
FT                   /label="ampicillin resistance"
FT   rep_origin      1533..1850
FT                   /label="pMB1 ori"
FT   misc_feature    1855..2044
FT                   /label="ConLS connector"
FT   promoter        2049..3046
FT                   /label="S. cerevisiae ENO1 promoter"
FT   CDS             3053..4087
FT                   /label="Cre"
FT   terminator      4088..4331
FT                   /label="AOX1 terminator"
FT   misc_feature    4336..4504
FT                   /label="ConR1 connector"
FT   promoter        4509..5065
FT                   /label="ILV5 promoter"
FT   promoter        5066..5130
FT                   /label="synthetic EM72 promoter"
FT   CDS             5131..5940
FT                   /label="Tn903 neomycin resistance"
FT   terminator      5945..6150
FT                   /label="E. gossypii TEF1 terminator"
FT   rep_origin      6161..6324
FT                   /label="Pichia ARS1"
SQ   SEQUENCE  6334 AA; 1957088 MW;
     GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA        60
     TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC       120
     CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA       180
     TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGGGACC       240
     CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA       300
     GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA       360
     GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG       420
     TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC       480
     GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG       540
     TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT       600
     CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT       660
     CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA       720
     ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC       780
     GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC       840
     CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA       900
     GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT       960
     TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT      1020
     TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC      1080
     CACCTGTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG      1140
     TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC      1200
     AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC      1260
     TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT      1320
     AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC      1380
     TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT      1440
     CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC      1500
     AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG      1560
     AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG      1620
     GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG      1680
     TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA      1740
     GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT      1800
     TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT CCCTGAATTC      1860
     GCATCTAGAT GGTAGAGCCA CAAACAGCCG GTACAAGCAA CGATCTCCAG GACCATCTGA      1920
     ATCATGCGCG GATGACACGA ACTCACGACG GCGATCACAG ACATTAACCC ACAGTACAGA      1980
     CACTGCGACA ACGTGGCAAT TCGTCGCAAT ACCGTCTCAC TGAACTGGCC GATAATTGCA      2040
     GACGAACGAG AAAGCATACT ATACTATTCG ACATTCCTTT CAATCCTGGA ATTAACAGTC      2100
     ACTTTTAAAA AAGACATCTA CCGTGAAGGT GCCGTAGAGT ATCGCGTTAC CATATCGCCA      2160
     AAAACTGATA TACGCCGCGG AAACCAGGCA AACAATTGAA AAGAAAAATT TTGAGGAACT      2220
     CTCTGCATCG AAGCCGTCTA GAGTTACCAC TAGTCAGATG CCGCGGGCAC TTGAGCACCT      2280
     CATGCACAGC AATAACACAA CACAATGGTT AGTAGCAACC TGAATTCGGT CATTGATGCA      2340
     TGCATGTGCC GTGAAGCGGG ACAACCAGAA AAGTCGTCTA TAAATGCCGG CACGTGCGAT      2400
     CATCGTGGCG GGGTTTTAAG AGTGCATATC ACAAATTGTC GCATTACCGC GGAACCGCCA      2460
     GATATTCATT ACTTGACGCA AAAGCGTTTG AAATAATGAC GAAAAAGAAG GAAGAAAAAA      2520
     AAAGAAAAAT ACCGCTTCTA GGCGGGTTAT CTACTGATCC GAGCTTCCAC TAGGATAGCA      2580
     CCCAAACACC TGCATATTTG GACGACCTTT ACTTACACCA CCAAAAACCA CTTTCGCCTC      2640
     TCCCGCCCCT GATAACGTCC ACTAATTGAG CGATTACCTG AGCGGTCCTC TTTTGTTTGC      2700
     AGCATGAGAC TTGCATACTG CAAATCGTAA GTAGCAACCT CTCAAGGTCA AAACTGTATG      2760
     GAAACCTTGT CACCTCACTT AATTCTAGCT AGCCTACCCT GCAAGTCAAG AGCTCTCCGT      2820
     GATTCCTAGC CACCTCAAGG TATGCCTCTC CCCGGAAACT GTGGCCTTTT CTGGCACACA      2880
     TGATCTCCAC GATTTCAACA TATAAATAGC TTTTGATAAT GGCAATATTA ATCAAATTTA      2940
     TTTTACTTCT TTCTTGTAAC ATCTCTCTTG TAATCCCTTA TTCCTTCTAG CTATTTTTCA      3000
     TAAAAAACCA AGCAACTGCT TATCAACACA CAAACACTAA ATCAAAAGAT CTATGTCCAA      3060
     TTTACTGACC GTACACCAAA ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT      3120
     TCGCAAGAAC CTGATGGACA TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA      3180
     AATGCTTCTG TCCGTTTGCC GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG      3240
     GTTTCCCGCA GAACCTGAAG ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT      3300
     GGCAGTAAAA ACTATCCAGC AACATTTGGG CCAGCTAAAC ATGCTTCATC GTCGGTCCGG      3360
     GCTGCCACGA CCAAGTGACA GCAATGCTGT TTCACTGGTT ATGCGGCGGA TCCGAAAAGA      3420
     AAACGTTGAT GCCGGTGAAC GTGCAAAACA GGCTCTAGCG TTCGAACGCA CTGATTTCGA      3480
     CCAGGTTCGT TCACTCATGG AAAATAGCGA TCGCTGCCAG GATATACGTA ATCTGGCATT      3540
     TCTGGGGATT GCTTATAACA CCCTGTTACG TATAGCCGAA ATTGCCAGGA TCAGGGTTAA      3600
     AGATATCTCA CGTACTGACG GTGGGAGAAT GTTAATCCAT ATTGGCAGAA CGAAAACGCT      3660
     GGTTAGCACC GCAGGTGTAG AGAAGGCACT TAGCCTGGGG GTAACTAAAC TGGTCGAGCG      3720
     ATGGATTTCC GTGTCTGGTG TAGCTGATGA TCCGAATAAC TACCTGTTTT GCCGGGTCAG      3780
     AAAAAATGGT GTTGCCGCGC CATCTGCCAC CAGCCAGCTA TCAACTCGCG CCCTGGAAGG      3840
     GATTTTTGAA GCAACTCATC GATTGATTTA CGGCGCTAAG GATGACTCTG GTCAGAGATA      3900
     CCTGGCCTGG TCTGGACACA GTGCCCGTGT CGGAGCCGCG CGAGATATGG CCCGCGCTGG      3960
     AGTTTCAATA CCGGAGATCA TGCAAGCTGG TGGCTGGACC AATGTAAATA TTGTCATGAA      4020
     CTATATCCGT AACCTGGATA GTGAAACAGG GGCAATGGTG CGCCTGCTGG AAGATGGCGG      4080
     ATCCTAATCA AGAGGATGTC AGAATGCCAT TTGCCTGAGA GATGCAGGCT TCATTTTTGA      4140
     TACTTTTTTA TTTGTAACCT ATATAGTATA GGATTTTTTT TGTCATTTTG TTTCTTCTCG      4200
     TACGAGCTTG CTCCTGATCA GCCTATCTCG CAGCTGATGA ATATCTTGTG GTAGGGGTTT      4260
     GGGAAAATCA TTCGAGTTTG ATGTTTTTCT TGGTATTTCC CACTCCTCTT CAGAGTACAG      4320
     AAGATTAAGT GGCTGCCAAT GAGACGACGG GGTCATCACG GCTCATCATG CGCCAAACAA      4380
     ATGTGTGCAA TACACGCTCG GATGACTGCA TGATGACCGC ACTGACTGGG GACAGCAGAT      4440
     CCACCTAAGC CTGTGAGAGA AGCAGACACC CGACAGATCA AGGCAGTTAA CTAGTGCACT      4500
     GCAGTACAAT CCTTCAGTAA TGTCTTGTTT CTTTTGTTGC AGTGGTGAGC CATTTTGACT      4560
     TCGTGAAAGT TTCTTTAGAA TAGTTGTTTC CAGAGGCCAA ACATTCCACC CGTAGTAAAG      4620
     TGCAAGCGTA GGAGAACCAA GACTGGCATA AATCAGGTAT AAGTGTCGAG CACTGGCAGG      4680
     TGATCTTCTG AAAGTTTCTA CTAGCAGATA AGATCCAGTA GTCATGCATA TGGCAACAAT      4740
     GTACCGTGTG GATCTAAGAA CGCGTCCTAC TAACCTTCGC ATTCGTTGGT CCAGTTTGTT      4800
     GTTATCGATC AACGTGACAA GGTTGTCGAT TCCGCGTAAG CATGCATACC CAAGGACGCC      4860
     TGTTGCAATT CCAAGTGAGC CAGTTCCAAC AATCTTTGTA ATATTAGAGC ACTTCATTGT      4920
     GTTGCGCTTG AAAGTAAAAT GCGAACAAAT TAAGAGATAA TCTCGAAACC GCGACTTCAA      4980
     ACGCCAATAT GATGTGCGGC ACACAATAAG CGTTCATATC CGCTGGGTGA CTTTCTCGCT      5040
     TTAAAAAATT ATCCGAAAAA ATTTTCTAGA GTGTTGTTAC TTTATACTTC CGGCTCGTAT      5100
     AATACGACAA GGTGTAAGGA GGACTAAACC ATGGGTAAGG AAAAGACTCA CGTTTCGAGG      5160
     CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT      5220
     GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC GCCAGAGTTG      5280
     TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA      5340
     AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT      5400
     GATGCATGGT TACTCACCAC TGCGATCCCC GGCAAAACAG CATTCCAGGT ATTAGAAGAA      5460
     TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT      5520
     TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGCCT GGCTCAGGCG      5580
     CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC      5640
     TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAGCTTT TGCCATTCTC ACCGGATTCA      5700
     GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA      5760
     GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA      5820
     TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT      5880
     ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGACGA GTTCTTCTAA      5940
     GCCGAGTAAC TGACTGACAA TAAAAAGATT CTTGTTTTCA AGAACTTGTC ATTTGTATAG      6000
     TTTTTTTATA TTGTAGTTGT TCTATTTTAA TCAAATGTTA GCGTGATTTA TATTTTTTTT      6060
     CGCCTCGACA TCATCTGCCC AGATGCGAAG TTAAGTGCGC AGAAAGTAAT ATCATGCGTC      6120
     AATCGTATGT GAATGCTGGT CGCTATACTG GAGTATGCAT TCGAGATAAG CTGGGGGAAC      6180
     ATTCGCGAAA ATGAAACAAG TCGGCTGTTA TAGTATATTT ATTATAATAT TGAAAGATCT      6240
     CAAAAGACTA CTTATTTTTG AATGAACCAA GTATGAAATC AACCTATTTG GGGTTGACCA      6300
     AAATAAGTAA ATATTAATTG TCGAATGCAT CCGA                                  6334
//
