ID   pPTK081_0_Empty; SV 1; circular; unknown; STD; UNC; 2676 BP.
DT   23-OCT-2023
DE   LMBP 12734.
CC   Accession number: LMBP 12734
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        13..142
FT                   /label="glpT promoter"
FT   RBS             143..178
FT                   /label="synthetic RBS"
FT   CDS             179..895
FT                   /label="sfGFP"
FT   terminator      907..950
FT                   /label="T1 terminator"
FT   terminator      984..1024
FT                   /label="T7Te terminator"
FT   CDS             complement(1147..1806)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      2358..2676
FT                   /label="pMB1 ori"
SQ   SEQUENCE  2676 AA;  827369 MW;
     GTCGGCGAGA CGGAAAGTGA AACGTGATTT CATGCGTCAT TTTGAACATT TTGTAAATCT        60
     TATTTAATAA TGTGTGCGGC AATTCACATT TAATTTATGA ATGTTTTCTT AACATCGCGG       120
     CAACTCAAGA AACGGCAGGT TCGGATCTTA GCTACTAGAG AAAGAGGAGA AATACTAGAT       180
     GCGTAAAGGC GAAGAGCTGT TCACTGGTGT CGTCCCTATT CTGGTGGAAC TGGATGGTGA       240
     TGTCAACGGT CATAAGTTTT CCGTGCGTGG CGAGGGTGAA GGTGACGCAA CTAATGGTAA       300
     ACTGACGCTG AAGTTCATCT GTACTACTGG TAAACTGCCG GTTCCTTGGC CGACTCTGGT       360
     AACGACGCTG ACTTATGGTG TTCAGTGCTT TGCTCGTTAT CCGGACCATA TGAAGCAGCA       420
     TGACTTCTTC AAGTCCGCCA TGCCGGAAGG CTATGTGCAG GAACGCACGA TTTCCTTTAA       480
     GGATGACGGC ACGTACAAAA CGCGTGCGGA AGTGAAATTT GAAGGCGATA CCCTGGTAAA       540
     CCGCATTGAG CTGAAAGGCA TTGACTTTAA AGAGGACGGC AATATCCTGG GCCATAAGCT       600
     GGAATACAAT TTTAACAGCC ACAATGTTTA CATCACCGCC GATAAACAAA AAAATGGCAT       660
     TAAAGCGAAT TTTAAAATTC GCCACAACGT GGAGGATGGC AGCGTGCAGC TGGCTGATCA       720
     CTACCAGCAA AACACTCCAA TCGGTGATGG TCCTGTTCTG CTGCCAGACA ATCACTATCT       780
     GAGCACGCAA AGCGTTCTGT CTAAAGATCC GAACGAGAAA CGCGATCATA TGGTTCTGCT       840
     GGAGTTCGTA ACCGCAGCGG GCATCACGCA TGGTATGGAT GAACTGTACA AATGACCAGG       900
     CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG       960
     TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT      1020
     TATACGTCTC TGACCAGACC AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA      1080
     TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCGAT      1140
     ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT      1200
     GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC      1260
     CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT      1320
     ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AAACGAAAAA      1380
     CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC      1440
     TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA      1500
     AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC      1560
     CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA GCATTCATCA GGCGGGCAAG      1620
     AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC      1680
     CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC      1740
     AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT      1800
     CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG      1860
     TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCCGATCA ATCATGACCA      1920
     AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG      1980
     GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC      2040
     CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA      2100
     CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC      2160
     ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG      2220
     TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC      2280
     CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC      2340
     GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC      2400
     CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA      2460
     CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC      2520
     TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG      2580
     CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT      2640
     TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTA                                2676
//
