ID   pPTK109_P2-4_sfGFP; SV 1; circular; unknown; STD; UNC; 2678 BP.
DT   23-OCT-2023
OS   unknown
CC   Accession number: LMBP 13775
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        10..139
FT                   /label="glpT promoter"
FT   RBS             140..175
FT                   /label="synthetic RBS"
FT   CDS             176..892
FT                   /label="sfGFP"
FT   terminator      904..947
FT                   /label="T1 terminator"
FT   terminator      981..1021
FT                   /label="T7Te terminator"
FT   CDS             complement(1144..1803)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      1985..2576
FT                   /label="pMB1 ori"
SQ   SEQUENCE  2678 AA;  828011 MW;
     TCTCAAACGG AAAGTGAAAC GTGATTTCAT GCGTCATTTT GAACATTTTG TAAATCTTAT        60
     TTAATAATGT GTGCGGCAAT TCACATTTAA TTTATGAATG TTTTCTTAAC ATCGCGGCAA       120
     CTCAAGAAAC GGCAGGTTCG GATCTTAGCT ACTAGAGAAA GAGGAGAAAT ACTAGATGCG       180
     TAAAGGCGAA GAGCTGTTCA CTGGTGTCGT CCCTATTCTG GTGGAACTGG ATGGTGATGT       240
     CAACGGTCAT AAGTTTTCCG TGCGTGGCGA GGGTGAAGGT GACGCAACTA ATGGTAAACT       300
     GACGCTGAAG TTCATCTGTA CTACTGGTAA ACTGCCGGTT CCTTGGCCGA CTCTGGTAAC       360
     GACGCTGACT TATGGTGTTC AGTGCTTTGC TCGTTATCCG GACCATATGA AGCAGCATGA       420
     CTTCTTCAAG TCCGCCATGC CGGAAGGCTA TGTGCAGGAA CGCACGATTT CCTTTAAGGA       480
     TGACGGCACG TACAAAACGC GTGCGGAAGT GAAATTTGAA GGCGATACCC TGGTAAACCG       540
     CATTGAGCTG AAAGGCATTG ACTTTAAAGA GGACGGCAAT ATCCTGGGCC ATAAGCTGGA       600
     ATACAATTTT AACAGCCACA ATGTTTACAT CACCGCCGAT AAACAAAAAA ATGGCATTAA       660
     AGCGAATTTT AAAATTCGCC ACAACGTGGA GGATGGCAGC GTGCAGCTGG CTGATCACTA       720
     CCAGCAAAAC ACTCCAATCG GTGATGGTCC TGTTCTGCTG CCAGACAATC ACTATCTGAG       780
     CACGCAAAGC GTTCTGTCTA AAGATCCGAA CGAGAAACGC GATCATATGG TTCTGCTGGA       840
     GTTCGTAACC GCAGCGGGCA TCACGCATGG TATGGATGAA CTGTACAAAT GACCAGGCAT       900
     CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG       960
     GTGAACGCTC TCTACTAGAG TCACACTGGC TCACCTTCGG GTGGGCCTTT CTGCGTTTAT      1020
     AGCTGTGAGA CCAGACCAAT AAAAAACGCC CGGCGGCAAC CGAGCGTTCT GAACAAATCC      1080
     AGATGGAGTT CTGAGGTCAT TACTGGATCT ATCAACAGGA GTCCAAGCGA GCTCGATATC      1140
     AAATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC      1200
     GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT      1260
     GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT      1320
     GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAAA CGAAAAACAT      1380
     ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG      1440
     CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA      1500
     CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG      1560
     CTCACCGTCT TTCATTGCCA TACGAAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT      1620
     GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT      1680
     AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA      1740
     ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC      1800
     CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA      1860
     TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CCGATCAATC ATGACCAAAA      1920
     TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT      1980
     CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC      2040
     TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG      2100
     GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC      2160
     ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG      2220
     CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG      2280
     ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA      2340
     CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG      2400
     AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA      2460
     GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT      2520
     GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA      2580
     GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC      2640
     CTGCGTTATC CCCTGATTCT GTGGATAACC GTAGTCGG                              2678
//
