ID   pPTK196_P8_HygR; SV 1; circular; unknown; STD; UNC; 3547 BP.
DT   16-OCT.-2023
DE   LMBP 12786.
CC   Accession number: LMBP 12786
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified:16-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   rep_origin      447..764
FT                   /label="pMB1 ori"
FT   promoter        778..831
FT                   /label="PLtetO-1 promoter"
FT   RBS             832..856
FT                   /label="synthetic RBS"
FT   CDS             857..1534
FT                   /label="mRFP1"
FT   terminator      1555..1598
FT                   /label="T1 terminator"
FT   terminator      1632..1672
FT                   /label="T7Te terminator"
FT   promoter        1688..2243
FT                   /label="ILV5 promoter"
FT   promoter        2244..2308
FT                   /label="synthetic EM72 promoter"
FT   CDS             2309..3337
FT                   /label="hygromycin resistance"
FT   terminator      3342..3547
FT                   /label="E. gossypii TEF1 terminator"
SQ   SEQUENCE  3547 AA; 1096931 MW;
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA        60
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA       120
     AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC       180
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT       240
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC       300
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC       360
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA       420
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG       480
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG       540
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT       600
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT       660
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC       720
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC       780
     CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA       840
     AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA       900
     AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG       960
     TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC      1020
     GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA      1080
     CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG      1140
     TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA      1200
     CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT      1260
     TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG      1320
     TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC      1380
     TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC      1440
     CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA      1500
     ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA      1560
     CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT      1620
     CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC      1680
     TCACCGAATC CTTCGTAATG TCTTGTTTCT TTTGTTGCAG TGGTGAGCCA TTTTGACTTC      1740
     GTGAAAGTTT CTTTAGAATA GTTGTTTCCA GAGGCCAAAC ATTCCACCCG TAGTAAAGTG      1800
     CAAGCGTAGG AGAACCAAGA CTGGCATAAA TCAGGTATAA GTGTCGAGCA CTGGCAGGTG      1860
     ATCTTCTGAA AGTTTCTACT AGCAGATAAG ATCCAGTAGT CATGCATATG GCAACAATGT      1920
     ACCGTGTGGA TCTAAGAACG CGTCCTACTA ACCTTCGCAT TCGTTGGTCC AGTTTGTTGT      1980
     TATCGATCAA CGTGACAAGG TTGTCGATTC CGCGTAAGCA TGCATACCCA AGGACGCCTG      2040
     TTGCAATTCC AAGTGAGCCA GTTCCAACAA TCTTTGTAAT ATTAGAGCAC TTCATTGTGT      2100
     TGCGCTTGAA AGTAAAATGC GAACAAATTA AGAGATAATC TCGAAACCGC GACTTCAAAC      2160
     GCCAATATGA TGTGCGGCAC ACAATAAGCG TTCATATCCG CTGGGTGACT TTCTCGCTTT      2220
     AAAAAATTAT CCGAAAAAAT TTTCTAGAGT GTTGTTACTT TATACTTCCG GCTCGTATAA      2280
     TACGACAAGG TGTAAGGAGG ACTAAACCAT GGGTAAAAAG CCTGAACTCA CCGCGACGTC      2340
     TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTGTCC GACCTGATGC AGCTCTCGGA      2400
     GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT      2460
     AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC      2520
     CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTT AGCGAGAGCC TGACCTATTG      2580
     CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC      2640
     TGTTCTGCAA CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC      2700
     GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT      2760
     CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT      2820
     CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA      2880
     AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG      2940
     CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC      3000
     CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA      3060
     GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG      3120
     TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA      3180
     GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC      3240
     CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA      3300
     CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAAGCC GAGTAACTGA CTGACAATAA      3360
     AAAGATTCTT GTTTTCAAGA ACTTGTCATT TGTATAGTTT TTTTATATTG TAGTTGTTCT      3420
     ATTTTAATCA AATGTTAGCG TGATTTATAT TTTTTTTCGC CTCGACATCA TCTGCCCAGA      3480
     TGCGAAGTTA AGTGCGCAGA AAGTAATATC ATGCGTCAAT CGTATGTGAA TGCTGGTCGC      3540
     TATACTG                                                                3547
//
