ID   pPTK077_P8_Lox71-NourR-Lox66; SV 1; circular; unknown; STD; UNC; 3210 BP.
DT   16-OCT.-2023
DE   LMBP 12791.
CC   Accession number: LMBP 12791
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 16-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   rep_origin      447..764
FT                   /label="pMB1 ori"
FT   promoter        778..831
FT                   /label="PLtetO-1 promoter"
FT   RBS             832..856
FT                   /label="synthetic RBS"
FT   CDS             857..1534
FT                   /label="mRFP1"
FT   terminator      1555..1598
FT                   /label="T1 terminator"
FT   terminator      1632..1672
FT                   /label="T7Te terminator"
FT   misc_recomb     1713..1746
FT                   /label="Lox71"
FT   promoter        1747..2303
FT                   /label="ILV5 promoter"
FT   promoter        2304..2368
FT                   /label="synthetic EM72 promoter"
FT   CDS             2369..2941
FT                   /label="nourseothricin resistance"
FT   terminator      2946..3151
FT                   /label="E. gossypii TEF1 terminator"
FT   misc_recomb     3152..3185
FT                   /label="Lox66"
SQ   SEQUENCE  3210 AA;  993005 MW;
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA        60
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA       120
     AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC       180
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT       240
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC       300
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC       360
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA       420
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG       480
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG       540
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT       600
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT       660
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC       720
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC       780
     CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA       840
     AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA       900
     AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG       960
     TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC      1020
     GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA      1080
     CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG      1140
     TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA      1200
     CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT      1260
     TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG      1320
     TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC      1380
     TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC      1440
     CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA      1500
     ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA      1560
     CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT      1620
     CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC      1680
     TCACCGAACA GGGACTAAAG AGGTGATGAT TATACCGTTC GTATAGCATA CATTATACGA      1740
     AGTTATATCC TTCAGTAATG TCTTGTTTCT TTTGTTGCAG TGGTGAGCCA TTTTGACTTC      1800
     GTGAAAGTTT CTTTAGAATA GTTGTTTCCA GAGGCCAAAC ATTCCACCCG TAGTAAAGTG      1860
     CAAGCGTAGG AGAACCAAGA CTGGCATAAA TCAGGTATAA GTGTCGAGCA CTGGCAGGTG      1920
     ATCTTCTGAA AGTTTCTACT AGCAGATAAG ATCCAGTAGT CATGCATATG GCAACAATGT      1980
     ACCGTGTGGA TCTAAGAACG CGTCCTACTA ACCTTCGCAT TCGTTGGTCC AGTTTGTTGT      2040
     TATCGATCAA CGTGACAAGG TTGTCGATTC CGCGTAAGCA TGCATACCCA AGGACGCCTG      2100
     TTGCAATTCC AAGTGAGCCA GTTCCAACAA TCTTTGTAAT ATTAGAGCAC TTCATTGTGT      2160
     TGCGCTTGAA AGTAAAATGC GAACAAATTA AGAGATAATC TCGAAACCGC GACTTCAAAC      2220
     GCCAATATGA TGTGCGGCAC ACAATAAGCG TTCATATCCG CTGGGTGACT TTCTCGCTTT      2280
     AAAAAATTAT CCGAAAAAAT TTTCTAGAGT GTTGTTACTT TATACTTCCG GCTCGTATAA      2340
     TACGACAAGG TGTAAGGAGG ACTAAACCAT GGGTACTACC TTAGATGATA CAGCCTACAG      2400
     ATACAGAACA TCAGTCCCTG GTGATGCTGA AGCAATTGAG GCTTTAGACG GTTCATTCAC      2460
     CACCGACACC GTCTTTAGAG TAACCGCCAC CGGTGATGGA TTTACCTTAA GAGAAGTCCC      2520
     AGTCGACCCT CCATTAACTA AAGTCTTTCC AGATGATGAA TCTGATGACG AAAGCGACGA      2580
     CGGAGAAGAT GGTGACCCAG ATTCAAGAAC TTTCGTAGCA TACGGTGATG ACGGTGATTT      2640
     GGCTGGTTTT GTAGTCGTTT CTTATTCAGG TTGGAATAGA AGGTTGACCG TTGAAGATAT      2700
     AGAAGTCGCC CCAGAGCATA GAGGTCATGG TGTAGGAAGA GCTTTGATGG GTTTGGCTAC      2760
     AGAATTTGCA AGAGAGAGAG GAGCCGGTCA TTTATGGTTA GAAGTTACTA ATGTTAACGC      2820
     CCCTGCTATC CATGCTTATA GAAGAATGGG TTTCACATTA TGTGGTTTAG ATACTGCTTT      2880
     ATATGATGGA ACAGCATCTG ACGGTGAACA GGCCTTGTAT ATGTCTATGC CTTGCCCTTA      2940
     AGCCGAGTAA CTGACTGACA ATAAAAAGAT TCTTGTTTTC AAGAACTTGT CATTTGTATA      3000
     GTTTTTTTAT ATTGTAGTTG TTCTATTTTA ATCAAATGTT AGCGTGATTT ATATTTTTTT      3060
     TCGCCTCGAC ATCATCTGCC CAGATGCGAA GTTAAGTGCG CAGAAAGTAA TATCATGCGT      3120
     CAATCGTATG TGAATGCTGG TCGCTATACT GATAACTTCG TATAGCATAC ATTATACGAA      3180
     CGGTAGACAG TATCGAATGG ACTCTGATGA                                       3210
//
