ID   pPTK204_P6_Lox71-HygR-Lox66; SV 1; circular; unknown; STD; UNC; 3595 BP.
DT   23-OCT-2023
DE   LMBP 12773.
CC   Accession number: LMBP 12773
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 23-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(112..771)
FT                   /label="chloramphenicol resistance"
FT   rep_origin      1323..1641
FT                   /label="pMB1 ori"
FT   misc_recomb     1656..1689
FT                   /label="Lox71"
FT   promoter        1690..2246
FT                   /label="ILV5 promoter"
FT   promoter        2247..2311
FT                   /label="synthetic EM72 promoter"
FT   CDS             2312..3340
FT                   /label="hygromycin resistance"
FT   terminator      3353..3550
FT                   /label="E. gossypii TEF1 terminator"
FT   misc_recomb     3551..3584
FT                   /label="Lox66"
SQ   SEQUENCE  3595 AA; 1111306 MW;
     AGACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT        60
     GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC       120
     GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC       180
     ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT       240
     ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA       300
     ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAAACG AAAAACATAT TCTCAATAAA       360
     CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG       420
     TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG       480
     CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT       540
     CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC       600
     CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG       660
     AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG       720
     ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC       780
     CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT       840
     ATGGTGAAAG TTGGAACCTC TTACGTGCCC GATCAATCAT GACCAAAATC CCTTAACGTG       900
     AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC       960
     CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG      1020
     TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG      1080
     CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT      1140
     CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG      1200
     GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC      1260
     GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG      1320
     AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG      1380
     CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG      1440
     GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC      1500
     GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT      1560
     TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC      1620
     CTGATTCTGT GGATAACCGT AGTCGGTCTC ATACATACCG TTCGTATAGC ATACATTATA      1680
     CGAAGTTATA TCCTTCAGTA ATGTCTTGTT TCTTTTGTTG CAGTGGTGAG CCATTTTGAC      1740
     TTCGTGAAAG TTTCTTTAGA ATAGTTGTTT CCAGAGGCCA AACATTCCAC CCGTAGTAAA      1800
     GTGCAAGCGT AGGAGAACCA AGACTGGCAT AAATCAGGTA TAAGTGTCGA GCACTGGCAG      1860
     GTGATCTTCT GAAAGTTTCT ACTAGCAGAT AAGATCCAGT AGTCATGCAT ATGGCAACAA      1920
     TGTACCGTGT GGATCTAAGA ACGCGTCCTA CTAACCTTCG CATTCGTTGG TCCAGTTTGT      1980
     TGTTATCGAT CAACGTGACA AGGTTGTCGA TTCCGCGTAA GCATGCATAC CCAAGGACGC      2040
     CTGTTGCAAT TCCAAGTGAG CCAGTTCCAA CAATCTTTGT AATATTAGAG CACTTCATTG      2100
     TGTTGCGCTT GAAAGTAAAA TGCGAACAAA TTAAGAGATA ATCTCGAAAC CGCGACTTCA      2160
     AACGCCAATA TGATGTGCGG CACACAATAA GCGTTCATAT CCGCTGGGTG ACTTTCTCGC      2220
     TTTAAAAAAT TATCCGAAAA AATTTTCTAG AGTGTTGTTA CTTTATACTT CCGGCTCGTA      2280
     TAATACGACA AGGTGTAAGG AGGACTAAAC CATGGGTAAA AAGCCTGAAC TCACCGCGAC      2340
     GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTG TCCGACCTGA TGCAGCTCTC      2400
     GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG      2460
     GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC      2520
     GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTTAGCGAGA GCCTGACCTA      2580
     TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC      2640
     CGCTGTTCTG CAACCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA      2700
     GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA      2760
     TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC      2820
     CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC      2880
     CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG      2940
     CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT      3000
     CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT      3060
     CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGGCTC CGGGCGTATA TGCTCCGCAT      3120
     TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC      3180
     GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT      3240
     CGCCCGCAGA AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG      3300
     AAACCGACGC CCCAGCACTC GTCCGAGGGC AAAGGAATAA GCCGAGTAAC TGACTGACAA      3360
     TAAAAAGATT CTTGTTTTCA AGAACTTGTC ATTTGTATAG TTTTTTTATA TTGTAGTTGT      3420
     TCTATTTTAA TCAAATGTTA GCGTGATTTA TATTTTTTTT CGCCTCGACA TCATCTGCCC      3480
     AGATGCGAAG TTAAGTGCGC AGAAAGTAAT ATCATGCGTC AATCGTATGT GAATGCTGGT      3540
     CGCTATACTG ATAACTTCGT ATAGCATACA TTATACGAAC GGTAGAGTTG AGACC           3595
//
