ID   pMC_MultiGene_NourR; SV 1; circular; unknown; STD; UNC; 4790 BP.
DT   17-OCT.-2023
DE   LMBP 12794.
CC   Accession number: LMBP 12794
CC   (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
CC   Last modified: 17-OCT-2023
CC   NGS data implemented
FH   Key             Location/Qualifiers
FH
FT   promoter        5..561
FT                   /label="ILV5 promoter"
FT   promoter        562..626
FT                   /label="synthetic EM72 promoter"
FT   CDS             627..1199
FT                   /label="nourseothricin resistance"
FT   terminator      1204..1409
FT                   /label="E. gossypii TEF1 terminator"
FT   CDS             complement(1652..2512)
FT                   /label="ampicillin resistance"
FT   rep_origin      3090..3407
FT                   /label="pMB1 ori"
FT   misc_feature    3412..3580
FT                   /label="ConLS' connector"
FT   promoter        3585..3714
FT                   /label="glpT promoter"
FT   RBS             3715..3750
FT                   /label="synthetic RBS"
FT   CDS             3751..4467
FT                   /label="sfGFP"
FT   terminator      4479..4522
FT                   /label="T1 terminator"
FT   terminator      4556..4596
FT                   /label="T7Te terminator"
FT   misc_feature    4601..4790
FT                   /label="ConRE' connector"
SQ   SEQUENCE  4790 AA; 1481017 MW;
     TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT        60
     GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA       120
     AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT       180
     CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC       240
     CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA       300
     TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT       360
     GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG       420
     CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC       480
     CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA       540
     AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA       600
     CGACAAGGTG TAAGGAGGAC TAAACCATGG GTACTACCTT AGATGATACA GCCTACAGAT       660
     ACAGAACATC AGTCCCTGGT GATGCTGAAG CAATTGAGGC TTTAGACGGT TCATTCACCA       720
     CCGACACCGT CTTTAGAGTA ACCGCCACCG GTGATGGATT TACCTTAAGA GAAGTCCCAG       780
     TCGACCCTCC ATTAACTAAA GTCTTTCCAG ATGATGAATC TGATGACGAA AGCGACGACG       840
     GAGAAGATGG TGACCCAGAT TCAAGAACTT TCGTAGCATA CGGTGATGAC GGTGATTTGG       900
     CTGGTTTTGT AGTCGTTTCT TATTCAGGTT GGAATAGAAG GTTGACCGTT GAAGATATAG       960
     AAGTCGCCCC AGAGCATAGA GGTCATGGTG TAGGAAGAGC TTTGATGGGT TTGGCTACAG      1020
     AATTTGCAAG AGAGAGAGGA GCCGGTCATT TATGGTTAGA AGTTACTAAT GTTAACGCCC      1080
     CTGCTATCCA TGCTTATAGA AGAATGGGTT TCACATTATG TGGTTTAGAT ACTGCTTTAT      1140
     ATGATGGAAC AGCATCTGAC GGTGAACAGG CCTTGTATAT GTCTATGCCT TGCCCTTAAG      1200
     CCGAGTAACT GACTGACAAT AAAAAGATTC TTGTTTTCAA GAACTTGTCA TTTGTATAGT      1260
     TTTTTTATAT TGTAGTTGTT CTATTTTAAT CAAATGTTAG CGTGATTTAT ATTTTTTTTC      1320
     GCCTCGACAT CATCTGCCCA GATGCGAAGT TAAGTGCGCA GAAAGTAATA TCATGCGTCA      1380
     ATCGTATGTG AATGCTGGTC GCTATACTGG AGTGTACTGA GAGTGCACCA TATGCGGTGT      1440
     GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG      1500
     CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCACCGAGAT      1560
     TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT      1620
     AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA      1680
     TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA      1740
     CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGGGACCCAC      1800
     GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA      1860
     GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG      1920
     TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG      1980
     TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG      2040
     TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG      2100
     TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC      2160
     TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT      2220
     TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA      2280
     CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA      2340
     AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA      2400
     ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC      2460
     AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC      2520
     TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG      2580
     AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC      2640
     CTGTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG      2700
     AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA      2760
     CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT      2820
     TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC      2880
     CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA      2940
     TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA      3000
     GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC      3060
     CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA      3120
     GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA      3180
     CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG      3240
     GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC      3300
     TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG      3360
     CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTCCC TGAATTCGCA      3420
     TCTAGACTGA TGAGACGTGG TAGAGCCACA AACAGCCGGT ACAAGCAACG ATCTCCAGGA      3480
     CCATCTGAAT CATGCGCGGA TGACACGAAC TCACGACGGC GATCACAGAC ATTAACCCAC      3540
     AGTACAGACA CTGCGACAAC GTGGCAATTC GTCGCAATAC AACGGAAAGT GAAACGTGAT      3600
     TTCATGCGTC ATTTTGAACA TTTTGTAAAT CTTATTTAAT AATGTGTGCG GCAATTCACA      3660
     TTTAATTTAT GAATGTTTTC TTAACATCGC GGCAACTCAA GAAACGGCAG GTTCGGATCT      3720
     TAGCTACTAG AGAAAGAGGA GAAATACTAG ATGCGTAAAG GCGAAGAGCT GTTCACTGGT      3780
     GTCGTCCCTA TTCTGGTGGA ACTGGATGGT GATGTCAACG GTCATAAGTT TTCCGTGCGT      3840
     GGCGAGGGTG AAGGTGACGC AACTAATGGT AAACTGACGC TGAAGTTCAT CTGTACTACT      3900
     GGTAAACTGC CGGTTCCTTG GCCGACTCTG GTAACGACGC TGACTTATGG TGTTCAGTGC      3960
     TTTGCTCGTT ATCCGGACCA TATGAAGCAG CATGACTTCT TCAAGTCCGC CATGCCGGAA      4020
     GGCTATGTGC AGGAACGCAC GATTTCCTTT AAGGATGACG GCACGTACAA AACGCGTGCG      4080
     GAAGTGAAAT TTGAAGGCGA TACCCTGGTA AACCGCATTG AGCTGAAAGG CATTGACTTT      4140
     AAAGAGGACG GCAATATCCT GGGCCATAAG CTGGAATACA ATTTTAACAG CCACAATGTT      4200
     TACATCACCG CCGATAAACA AAAAAATGGC ATTAAAGCGA ATTTTAAAAT TCGCCACAAC      4260
     GTGGAGGATG GCAGCGTGCA GCTGGCTGAT CACTACCAGC AAAACACTCC AATCGGTGAT      4320
     GGTCCTGTTC TGCTGCCAGA CAATCACTAT CTGAGCACGC AAAGCGTTCT GTCTAAAGAT      4380
     CCGAACGAGA AACGCGATCA TATGGTTCTG CTGGAGTTCG TAACCGCAGC GGGCATCACG      4440
     CATGGTATGG ATGAACTGTA CAAATGACCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA      4500
     AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA      4560
     CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGCTG GAAATCTGCT CGTCAGTGGT      4620
     GCTCACACTG ACGAATCATG TACAGATCAT ACCGATGACT GCCTGGCGAC TCACAACTAA      4680
     GCAAGACAGC CGGAACCAGC GCCGGCGAAC ACCACTGCAT ATATGGCATA TCACAACAGT      4740
     CCACGTCTCA AGCAGTTACA GAGATGTTAC GAACCACTAG TGCACTGCAG                 4790
//
