LOCUS       pENTR221-hPKCbeta1-no_stop      4560 bp    DNA     circular SYN 2-SEP-2003
DEFINITION  LMBP 10015.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     source          1..4560
                     /vntifkey="98"
     terminator      268..295
                     /vntifkey="43"
                     /label="rrnB T2 transcription terminator"
     terminator      427..470
                     /vntifkey="43"
                     /label="rrnB T1 transcription terminator"
     primer          517..533
                     /vntifkey="27"
                     /label="M13 (-40) forward primer"
     primer          537..552
                     /vntifkey="27"
                     /label="M13 (-20) forward primer"
     misc_recomb     569..668
                     /vntifkey="86"
                     /label=attL1
     CDS             671..2683
                     /label=hPKCbeta1
     misc_recomb     complement(2685..2784)
                     /vntifkey="86"
                     /label=attL2
     primer          complement(2801..2820)
                     /vntifkey="27"
                     /label="T7 primer"
     promoter        complement(2804..2820)
                     /vntifkey="30"
                     /label="T7 promoter"
     primer          complement(2825..2841)
                     /vntifkey="27"
                     /label="M13 reverse primer"
     CDS             2954..3763
                     /vntifkey="4"
                     /label=Kan(R)
     rep_origin      3884..4557
                     /vntifkey="33"
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCACC ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC
      721 CGTGCGCTTC GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCATGAGG TCAAGAACCA
      781 CAAATTCACC GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT
      841 CTGGGGCTTC GGGAAGCAGG GATTCCAGTG CCAAGTTTGC TGCTTTGTGG TGCACAAGCG
      901 GTGCCATGAA TTTGTCACAT TCTCCTGCCC TGGCGCTGAC AAGGGTCCAG CCTCCGATGA
      961 CCCCCGCAGC AAACACAAGT TTAAGATCCA CACGTACTCC AGCCCCACGT TTTGTGACCA
     1021 CTGTGGGTCA CTGCTGTATG GACTCATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT
     1081 GAATGTGCAC AAGCGCTGCG TGATGAATGT TCCCAGCCTG TGTGGCACGG ACCACACGGA
     1141 GCGCCGCGGC CGCATCTACA TCCAGGCCCA CATCGACAGG GACGTCCTCA TTGTCCTCGT
     1201 AAGAGATGCT AAAAACCTTG TACCTATGGA CCCCAATGGC CTGTCAGATC CCTACGTAAA
     1261 ACTGAAACTG ATTCCCGATC CCAAAAGTGA GAGCAAACAG AAGACCAAAA CCATCAAATG
     1321 CTCCCTCAAC CCTGAGTGGA ATGAGACATT TAGATTTCAG CTGAAAGAAT CGGACAAAGA
     1381 CAGAAGACTG TCAGTAGAGA TTTGGGATTG GGATTTGACC AGCAGGAATG ACTTCATGGG
     1441 ATCTTTGTCC TTTGGGATTT CTGAACTTCA GAAAGCCAGT GTTGATGGCT GGTTTAAGTT
     1501 ACTGAGCCAG GAGGAAGGCG AGTACTTCAA TGTGCCTGTG CCACCAGAAG GAAGTGAGGC
     1561 CAATGAAGAA CTGCGGCAGA AATTTGAGAG GGCCAAGATC AGTCAGGGAA CCAAGGTCCC
     1621 GGAAGAAAAG ACGACCAACA CTGTCTCCAA ATTTGACAAC AATGGCAACA GAGACCGGAT
     1681 GAAACTGACC GATTTTAACT TCCTAATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT
     1741 GCTTTCAGAA CGAAAAGGCA CAGATGAGCT CTATGCTGTG AAGATCCTGA AGAAGGACGT
     1801 TGTGATCCAA GATGATGACG TGGAGTGCAC TATGGTGGAG AAGCGGGTGT TGGCCCTGCC
     1861 TGGGAAGCCG CCCTTCCTGA CCCAGCTCCA CTCCTGCTTC CAGACCATGG ACCGCCTGTA
     1921 CTTTGTGATG GAGTACGTGA ATGGGGGCGA CCTCATGTAT CACATCCAGC AAGTCGGCCG
     1981 GTTCAAGGAG CCCCATGCTG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT
     2041 ACAGAGTAAG GGCATCATTT ACCGTGACCT AAAACTTGAC AACGTGATGC TCGATTCTGA
     2101 GGGACACATC AAGATTGCCG ATTTTGGCAT GTGTAAGGAA AACATCTGGG ATGGGGTGAC
     2161 AACCAAGACA TTCTGTGGCA CTCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC
     2221 CTATGGGAAG TCCGTGGATT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG
     2281 GCAGGCACCC TTTGAAGGGG AGGATGAAGA TGAACTCTTC CAATCCATCA TGGAACACAA
     2341 CGTAGCCTAT CCCAAGTCTA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC
     2401 CAAACACCCA GGCAAACGTC TGGGTTGTGG ACCTGAAGGC GAACGTGATA TCAAAGAGCA
     2461 TGCATTTTTC CGGTATATTG ATTGGGAGAA ACTTGAACGC AAAGAGATCC AGCCCCCTTA
     2521 TAAGCCAAAA GCTAGAGACA AGAGAGACAC CTCCAACTTC GACAAAGAGT TCACCAGACA
     2581 GCCTGTGGAA CTGACCCCCA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT
     2641 TGCTGGCTTC TCTTATACTA ACCCAGAGTT TGTCATTAAT GTGCACCCAG CTTTCTTGTA
     2701 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT
     2761 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA
     2821 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT
     2881 TGCACAAGAT AAAAATATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA
     2941 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA
     3001 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC
     3061 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA
     3121 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT
     3181 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC
     3241 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA
     3301 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA
     3361 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA
     3421 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT
     3481 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA
     3541 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG
     3601 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA
     3661 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT
     3721 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG
     3781 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA
     3841 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA
     3901 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC
     3961 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT
     4021 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG
     4081 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC
     4141 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT
     4201 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA
     4261 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT
     4321 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG
     4381 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA
     4441 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA
     4501 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//

