LOCUS       pEF6-P2A-mcrA           7094 bp    DNA     circular UNA 30-SEP-2016
DEFINITION  DMB 3964  (Invitrogen vector).
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|-11437314|
COMMENT     VNTNAME|pEF6-P2A-mcrA|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-P2A-mcrA" 0 7094 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "AarI" "AatII" "Acc65I" "AccI" "AceIII" "AciI" "AclI" 
COMMENT         "AcuI" "AfeI" "AflII" "AflIII" "AgeI" "AhdI" "AleI" "AloI" "AluI" 
COMMENT         "AlwI" "AlwNI" "ApaBI" "ApaI" "ApaLI" "ApoI" "AscI" "AseI" "AsiSI" 
COMMENT         "AvaI" "AvaII" "AvrII" "BaeI" "BamHI" "BanI" "BanII" "BbeI" "Bbr7I" 
COMMENT         "BbsI" "BbvCI" "BbvI" "BccI" "BceAI" "BcefI" "BcgI" "BciVI" "BclI" 
COMMENT         "BfaI" "BfrBI" "BglI" "BglII" "BlpI" "Bme1580I" "BmgBI" "BmrI" "BmtI" 
COMMENT         "BplI" "BpmI" "Bpu10I" "BpuEI" "BsaAI" "BsaBI" "BsaHI" "BsaI" "BsaJI" 
COMMENT         "BsaWI" "BsaXI" "BscAI" "BseMII" "BseRI" "BseYI" "BsgI" "BsiEI" 
COMMENT         "BsiHKAI" "BsiWI" "BslI" "BsmAI" "BsmBI" "BsmFI" "BsmI" "Bsp1286I" 
COMMENT         "Bsp24I" "BspCNI" "BspEI" "BspHI" "BspMI" "BsrBI" "BsrDI" "BsrFI" 
COMMENT         "BsrGI" "BsrI" "BssHII" "BssSI" "BstAPI" "BstBI" "BstEII" "BstF5I" 
COMMENT         "BstKTI" "BstNI" "BstUI" "BstXI" "BstYI" "BstZ17I" "Bsu36I" "BtgI" 
COMMENT         "BthCI" "BtsI" "Cac8I" "CdiI" "ChaI" "CjeI" "CjePI" "ClaI" "Csp6I" 
COMMENT         "CviAII" "CviJI" "DdeI" "DpnI" "DraI" "DraIII" "DrdI" "EaeI" "EagI" 
COMMENT         "EarI" "EciI" "Eco57MI" "EcoHI" "EcoICRI" "EcoNI" "EcoO109I" "EcoRI" 
COMMENT         "EcoRV" "EsaBC3I" "FalI" "FatI" "FauI" "FmuI" "Fnu4HI" "FokI" "FseI" 
COMMENT         "FspAI" "FspI" "GdiII" "HaeI" "HaeII" "HaeIII" "HaeIV" "HgaI" "HhaI" 
COMMENT         "Hin4I" "HincII" "HindIII" "HinfI" "HinP1I" "HpaI" "HpaII" "HphI" 
COMMENT         "Hpy188I" "Hpy188III" "Hpy8I" "Hpy99I" "HpyAV" "HpyCH4I" "HpyCH4III" 
COMMENT         "HpyCH4IV" "HpyCH4V" "HpyF10VI" "KasI" "KpnI" "LpnI" "MaeIII" "MboI" 
COMMENT         "MboII" "MfeI" "MluI" "MlyI" "MmeI" "MnlI" "MscI" "MseI" "MslI" 
COMMENT         "MspA1I" "MwoI" "NaeI" "NarI" "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" 
COMMENT         "NlaIII" "NlaIV" "Nli3877I" "NotI" "NruI" "NsiI" "NspI" "PacI" "PciI" 
COMMENT         "PflMI" "PfoI" "PleI" "PmeI" "PmlI" "PpiI" "Ppu10I" "PpuMI" "PshAI" 
COMMENT         "PsiI" "Psp03I" "PspGI" "PspOMI" "PsrI" "PssI" "PstI" "PvuI" "PvuII" 
COMMENT         "RleAI" "RsaI" "RsrII" "SacI" "SacII" "SalI" "SanDI" "SapI" "Sau96I" 
COMMENT         "SbfI" "ScaI" "SciI" "ScrFI" "SelI" "SexAI" "SfaNI" "SfcI" "SfiI" 
COMMENT         "SfoI" "SgrAI" "SimI" "SmaI" "SmlI" "SnaBI" "SpeI" "SphI" "SrfI" 
COMMENT         "Sse232I" "Sse8647I" "SspD5I" "SspI" "Sth132I" "StsI" "StuI" "StyD4I" 
COMMENT         "StyI" "SwaI" "TaiI" "TaqI" "TaqII" "TatI" "TauI" "TfiI" "TseI" 
COMMENT         "Tsp45I" "Tsp509I" "TspDTI" "TspGWI" "TspRI" "Tth111I" "Tth111II" 
COMMENT         "UnbI" "UthSI" "VpaK11AI" "XbaI" "XcmI" "XhoI" "XmaI" "XmnI" "ZraI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 2 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7094 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AarI" "cacctgc" 12 16 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 2424 0 7094 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 1 1592 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AccI" "gtmkac" 3 0 5 2265 0 2658 0 4910 0 4917 
COMMENT            0 7094 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AceIII" "cagctc" 14 18 4 3724 1 4335 0 5242 0 
COMMENT            6471 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AciI" "ccgc" 2 4 118 62 0 452 1 489 0 569 0 
COMMENT            603 0 605 1 786 0 789 0 910 1 950 0 952 1 956 1 999 1 1014 1 1081 0 
COMMENT            1096 0 1105 1 1141 1 1153 0 1194 1 1209 1 1213 1 1620 1 1689 1 1693 
COMMENT            0 1772 1 1783 1 1805 1 1827 0 1830 0 1832 1 1845 0 1930 1 1933 1 
COMMENT            1958 1 1963 0 1999 1 2019 0 2024 0 2039 0 2088 1 2091 1 2096 1 2110 
COMMENT            0 2127 0 2143 1 2160 0 2170 0 2216 1 2221 0 2223 1 2226 1 2295 1 
COMMENT            2312 1 2325 0 2432 0 2439 1 2461 0 2475 1 2510 0 2548 1 2620 0 2716 
COMMENT            1 2727 1 2744 0 2769 1 2780 1 2874 1 2931 0 3020 0 3026 1 3051 0 
COMMENT            3277 1 3301 1 3350 1 3364 1 3367 1 3395 0 3422 0 3942 0 3954 0 3963 
COMMENT            0 3975 0 3985 0 3996 0 4042 0 4342 0 4424 1 4491 1 4675 0 4678 0 
COMMENT            4744 1 4980 0 5083 0 5139 1 5149 1 5173 0 5216 1 5223 1 5244 1 5324 
COMMENT            0 5352 0 5479 0 5498 0 5619 0 5729 1 5864 0 5873 1 6235 0 6326 0 
COMMENT            6517 1 6563 0 6684 1 6728 0 6805 0 6914 0 7013 1 7060 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AclI" "aacgtt" 3 0 3 545 0 6398 0 6771 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AcuI" "ctgaag" 23 21 6 1421 0 1618 1 1664 0 
COMMENT            4320 0 5827 0 6839 1 0 "") 
COMMENT        #8=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 731 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AflIII" "acrygt" 2 0 7 229 0 985 0 2625 0 
COMMENT             2994 0 4153 0 4649 0 5290 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 430 0 1880 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 1 6172 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AleI" "cacnnnngtg" 6 0 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "AloI" "gaacnnnnnntcc" 26 21 2 2981 1 3607 1 0 
COMMENT             "") 
COMMENT        #15=(CRSite (CStringList) "AluI" "agct" 3 0 35 136 0 268 0 314 0 690 0 
COMMENT             1048 0 1178 0 1322 0 1414 0 1538 0 1575 0 1588 0 1600 0 1625 0 3314 
COMMENT             0 3481 0 3738 0 4058 0 4112 0 4122 0 4325 0 4430 0 4794 0 4927 0 
COMMENT             4933 0 4955 0 5050 0 5114 0 5232 0 5447 0 5537 0 5583 0 5840 0 6361 
COMMENT             0 6461 0 6524 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AlwI" "ggatc" 10 11 19 13 0 16 1 676 1 1470 0 
COMMENT             1599 1 1612 0 1985 0 4148 0 4538 0 4727 1 4756 0 5840 1 5926 1 5928 
COMMENT             0 6024 1 6025 0 6488 1 6805 0 6809 1 0 "") 
COMMENT        #17=(CRSite (CStringList) "AlwNI" "cagnnnctg" 7 0 2 81 0 5695 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "ApaBI" "gcannnnntgc" 9 0 4 1133 0 2504 0 3840 
COMMENT             0 3912 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 969 0 1720 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 33 0 5593 0 6839 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "ApoI" "raatty" 2 0 5 128 0 855 0 1441 0 3751 
COMMENT             0 4829 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "AseI" "attaat" 3 0 5 3765 0 4165 0 5061 0 
COMMENT             5120 0 6344 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 397 0 499 0 685 0 1200 0 
COMMENT             1317 0 2369 0 4115 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 13 320 0 1676 0 1729 0 
COMMENT             1889 0 2193 0 2218 0 2356 0 2525 0 2681 0 2752 0 4382 0 6310 0 6532 
COMMENT             0 0 "") 
COMMENT        #27=(CRSite (CStringList) "AvrII" "cctagg" 2 0 1 4094 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "BaeI" "acnnnngtayc" 24 19 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1604 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 16 358 0 433 0 806 0 1065 
COMMENT             0 1125 0 1290 0 1301 0 1592 0 2695 0 2801 0 2829 0 3133 0 3527 0 
COMMENT             4497 0 5034 0 6120 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "BanII" "grgcyc" 6 0 12 741 0 969 0 1180 0 
COMMENT             1602 0 1720 0 2034 0 2304 0 2592 0 3286 0 3322 0 3497 0 4487 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "BbeI" "ggcgcc" 6 0 4 1294 0 2699 0 2805 0 
COMMENT             2833 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "Bbr7I" "gaagac" 14 18 2 3262 0 4313 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "BbsI" "gaagac" 9 13 2 3257 0 4308 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "BbvCI" "cctcagc" 3 6 1 1254 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "BbvI" "gcagc" 14 18 28 188 1 323 0 650 1 813 
COMMENT             1 858 1 1155 1 2133 1 2322 1 2379 1 2421 1 2841 1 3398 0 4334 0 
COMMENT             4402 1 4405 1 4408 1 4439 0 4578 1 4803 0 5101 1 5182 1 5200 1 5608 
COMMENT             1 5698 0 5701 0 5907 0 6210 1 6601 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BccI" "ccatc" 10 11 17 272 1 1142 1 1362 1 
COMMENT             1707 1 1867 1 1930 1 2371 1 2537 0 3096 0 3553 1 3585 0 3966 0 4301 
COMMENT             0 4550 1 6216 0 6340 0 6627 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "BceAI" "acggc" 18 20 24 523 1 574 1 1068 1 
COMMENT             1243 1 1278 1 1874 0 1898 1 1986 0 2062 0 2156 0 2182 0 2202 0 2308 
COMMENT             0 2506 0 2587 1 2639 1 2683 1 2789 1 2817 1 3541 0 4287 0 4354 0 
COMMENT             4584 0 5781 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "BcefI" "acggc" 18 19 24 524 1 575 1 1069 1 
COMMENT             1244 1 1279 1 1874 0 1899 1 1986 0 2062 0 2156 0 2182 0 2202 0 2308 
COMMENT             0 2506 0 2588 1 2640 1 2684 1 2790 1 2818 1 3541 0 4287 0 4354 0 
COMMENT             4584 0 5781 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BcgI" "cgannnnnntgc" 25 23 2 2245 1 6711 0 0 
COMMENT             "") 
COMMENT        #41=(CRSite (CStringList) "BciVI" "gtatcc" 13 12 4 1733 1 3341 0 5482 1 
COMMENT             7009 1 0 "") 
COMMENT        #42=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 3055 0 4145 0 0 "") 
COMMENT        #43=(CRSite (CStringList) "BfaI" "ctag" 2 0 18 251 0 327 0 438 0 844 0 
COMMENT             1580 0 1611 0 2052 0 3077 0 3323 0 3415 0 4095 0 4332 0 4864 0 4924 
COMMENT             0 4928 0 5774 0 6027 0 6362 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "BfrBI" "atgcat" 4 0 2 3842 0 3914 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 5 1123 0 1299 0 1416 
COMMENT             0 2118 0 6292 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "BglII" "agatct" 2 0 2 13 0 918 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "BlpI" "gctnagc" 3 0 2 2633 0 4618 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "Bme1580I" "gkgcmc" 6 0 9 37 0 363 0 969 0 
COMMENT             1720 0 1993 0 2370 0 4412 0 5597 0 6843 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "BmgBI" "cacgtc" 4 4 4 1660 1 2182 1 2595 0 
COMMENT             3039 1 0 "") 
COMMENT        #50=(CRSite (CStringList) "BmrI" "actggg" 12 11 4 471 0 3973 1 4386 0 
COMMENT             6212 1 0 "") #51=(CRSite (CStringList) "BmtI" "gctagc" 6 0 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "BplI" "gagnnnnnctc" 25 0 1 4061 0 0 "") 
COMMENT        #53=(CRSite (CStringList) "BpmI" "ctggag" 23 21 4 1674 0 4722 1 4779 0 
COMMENT             6242 1 0 "") 
COMMENT        #54=(CRSite (CStringList) "Bpu10I" "cctnagc" 3 6 2 181 1 1254 0 0 "") 
COMMENT        #55=(CRSite (CStringList) "BpuEI" "cttgag" 23 21 10 776 0 1028 1 1462 1 
COMMENT             1742 0 4224 1 4501 0 5370 1 5668 0 5909 1 6777 1 0 "") 
COMMENT        #56=(CRSite (CStringList) "BsaAI" "yacgtr" 4 0 7 672 0 1795 0 2137 0 
COMMENT             2365 0 3568 0 4154 0 4652 0 0 "") 
COMMENT        #57=(CRSite (CStringList) "BsaBI" "gatnnnnatc" 6 0 1 4521 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "BsaHI" "grcgyc" 3 0 11 1291 0 1749 0 1911 0 
COMMENT             2381 0 2421 0 2696 0 2802 0 2830 0 4720 0 6709 0 7091 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 3 1560 1 2828 0 6233 1 0 
COMMENT             "") 
COMMENT        #60=(CRSite (CStringList) "BsaJI" "ccnngg" 2 0 31 500 0 603 0 772 0 950 
COMMENT             0 1099 0 1100 0 1277 0 1436 0 1672 0 1830 0 1863 0 1986 0 2221 0 
COMMENT             2328 0 2370 0 2565 0 2673 0 2817 0 2861 0 3125 0 3806 0 3878 0 4001 
COMMENT             0 4036 0 4094 0 4115 0 4221 0 4526 0 4633 0 5029 0 5439 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "BsaWI" "wccggw" 2 0 7 355 0 430 0 1880 0 2683 
COMMENT             0 5485 0 5632 0 6463 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "BsaXI" "acnnnnnctcc" 22 19 4 2366 1 2547 0 
COMMENT             3607 1 5135 1 0 "") 
COMMENT        #63=(CRSite (CStringList) "BscAI" "gcatc" 10 12 16 51 1 2471 0 2644 1 
COMMENT             3183 0 3267 1 3850 0 3922 0 4295 0 4352 0 4484 0 4530 0 4830 0 5375 
COMMENT             0 6427 0 6622 1 6867 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "BseMII" "ctcag" 16 14 13 54 0 101 1 1268 0 
COMMENT             1376 1 1498 0 2624 1 3178 1 3287 1 4045 1 5568 0 5977 0 6143 0 6660 
COMMENT             1 0 "") 
COMMENT        #65=(CRSite (CStringList) "BseRI" "gaggag" 17 15 5 1679 0 2387 0 2525 1 
COMMENT             4090 0 4650 0 0 "") 
COMMENT        #66=(CRSite (CStringList) "BseYI" "cccagc" 2 6 10 779 1 977 0 1756 1 
COMMENT             2104 1 3268 1 3314 1 3815 0 3887 0 4405 1 5583 1 0 "") 
COMMENT        #67=(CRSite (CStringList) "BsgI" "gtgcag" 23 21 4 548 0 907 1 2321 1 
COMMENT             4410 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "BsiEI" "cgrycg" 5 0 17 1123 0 1693 0 1776 0 
COMMENT             1787 0 1809 0 1905 0 2170 0 2190 0 2510 0 2620 0 2851 0 4342 0 4458 
COMMENT             0 5206 0 5619 0 6542 0 6691 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "BsiHKAI" "gwgcwc" 6 0 9 37 0 1180 0 1602 0 
COMMENT             1703 0 2499 0 2870 0 5597 0 6758 0 6843 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "BsiWI" "cgtacg" 2 0 4 2137 0 2604 0 2613 0 
COMMENT             2733 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "BslI" "ccnnnnnnngg" 8 0 40 368 0 369 0 443 0 
COMMENT             505 0 506 0 621 0 692 0 773 0 1027 0 1100 0 1101 0 1159 0 1171 0 
COMMENT             1232 0 1685 0 1726 0 1782 0 1836 0 1969 0 2045 0 2062 0 2063 0 2212 
COMMENT             0 2251 0 2275 0 2438 0 2489 0 2690 0 2715 0 2961 0 2962 0 3349 0 
COMMENT             3675 0 4002 0 4494 0 4745 0 5138 0 5319 0 5485 0 5764 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "BsmAI" "gtctc" 7 11 10 826 0 845 0 1046 0 
COMMENT             1390 1 1560 1 1650 1 2828 0 4242 0 6233 1 7009 0 0 "") 
COMMENT        #73=(CRSite (CStringList) "BsmBI" "cgtctc" 8 12 1 1650 1 0 "") 
COMMENT        #74=(CRSite (CStringList) "BsmFI" "gggac" 16 20 14 379 1 960 1 1773 0 
COMMENT             2112 0 2206 0 2408 1 2564 0 2738 1 2792 1 3788 1 3860 1 3924 1 4395 
COMMENT             0 4731 0 0 "") 
COMMENT        #75=(CRSite (CStringList) "BsmI" "gaatgc" 8 6 1 4859 1 0 "") 
COMMENT        #76=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 23 37 0 363 0 741 0 
COMMENT             969 0 1180 0 1602 0 1703 0 1720 0 1993 0 2034 0 2304 0 2370 0 2499 
COMMENT             0 2592 0 2870 0 3286 0 3322 0 3497 0 4412 0 4487 0 5597 0 6758 0 
COMMENT             6843 0 0 "") 
COMMENT        #77=(CRSite (CStringList) "Bsp24I" "gacnnnnnntgg" 25 20 6 1591 0 2227 0 
COMMENT             4590 0 4742 0 5982 0 7076 1 0 "") 
COMMENT        #78=(CRSite (CStringList) "BspCNI" "ctcag" 15 13 13 53 0 102 1 1267 0 
COMMENT             1377 1 1497 0 2625 1 3179 1 3288 1 4046 1 5567 0 5976 0 6142 0 6661 
COMMENT             1 0 "") #79=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "") 
COMMENT        #80=(CRSite (CStringList) "BspHI" "tcatga" 2 0 2 5999 0 7007 0 0 "") 
COMMENT        #81=(CRSite (CStringList) "BspMI" "acctgc" 11 15 3 876 0 2421 0 2850 0 0 
COMMENT             "") 
COMMENT        #82=(CRSite (CStringList) "BsrBI" "ccgctc" 4 4 7 1141 1 1209 1 2021 0 
COMMENT             3424 0 4982 0 5223 1 7013 1 0 "") 
COMMENT        #83=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 3 1712 0 6233 0 6407 1 0 
COMMENT             "") 
COMMENT        #84=(CRSite (CStringList) "BsrFI" "rccggy" 2 0 13 430 0 1052 0 1786 0 
COMMENT             1880 0 2112 0 2482 0 2850 0 2870 0 2894 0 3022 0 3463 0 4722 0 6252 
COMMENT             0 0 "") #85=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #86=(CRSite (CStringList) "BsrI" "actgg" 7 5 18 75 1 466 0 487 0 933 0 
COMMENT             1129 1 3657 0 3979 1 4357 0 4381 0 5090 1 5687 0 5700 0 5812 1 6218 
COMMENT             1 6336 1 6379 1 6648 0 6818 1 0 "") 
COMMENT        #87=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 0 0 "") 
COMMENT        #88=(CRSite (CStringList) "BssSI" "cacgag" 2 6 5 749 1 2585 0 4397 1 
COMMENT             5452 1 6836 1 0 "") 
COMMENT        #89=(CRSite (CStringList) "BstAPI" "gcannnnntgc" 8 0 4 1132 0 2503 0 
COMMENT             3839 0 3911 0 0 "") 
COMMENT        #90=(CRSite (CStringList) "BstBI" "ttcgaa" 3 0 0 0 "") 
COMMENT        #91=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 2 2155 0 2278 0 0 "") 
COMMENT        #92=(CRSite (CStringList) "BstF5I" "ggatg" 8 6 11 1718 0 1941 0 2529 1 
COMMENT             3279 0 3958 1 4287 1 4522 1 4735 0 6151 1 6332 1 6619 1 0 "") 
COMMENT        #93=(CRSite (CStringList) "BstKTI" "gatc" 4 0 29 8 0 16 0 24 0 684 0 921 
COMMENT             0 1465 0 1607 0 1980 0 3058 0 4143 0 4148 0 4458 0 4519 0 4533 0 
COMMENT             4735 0 4751 0 5848 0 5923 0 5934 0 5942 0 6020 0 6032 0 6137 0 6478 
COMMENT             0 6496 0 6542 0 6800 0 6817 0 6853 0 0 "") 
COMMENT        #94=(CRSite (CStringList) "BstNI" "ccwgg" 3 0 20 611 0 660 0 768 0 773 0 
COMMENT             1070 0 1101 0 1299 0 1674 0 2153 0 2246 0 2675 0 3127 0 3808 0 3863 
COMMENT             0 3880 0 4527 0 5030 0 5307 0 5428 0 5441 0 0 "") 
COMMENT        #95=(CRSite (CStringList) "BstUI" "cgcg" 3 0 41 121 0 209 0 231 0 452 0 
COMMENT             605 0 791 0 815 0 952 0 1014 0 1078 0 1194 0 1783 0 1832 0 1930 0 
COMMENT             1958 0 1965 0 1967 0 2036 0 2096 0 2178 0 2223 0 2312 0 2439 0 2517 
COMMENT             0 2612 0 2732 0 2775 0 2780 0 3340 0 3364 0 3384 0 3760 0 4454 0 
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COMMENT                                             " N1: 2245 (on complementary strand)" 
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COMMENT                              "BcgI: 2 sites") 24 22 "CGANNNNNNTGCNNNNNNNNNNNNN" 
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COMMENT                              "BclI: 2 sites") 1 5 "TGATCA" "ACTAGT") 
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COMMENT                               (CObjectList)) "BglI: 5 sites") 7 4 "GCCNNNNNGGC" 
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COMMENT                              "BglII: 2 sites") 1 5 "AGATCT" "TCTAGA") 
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COMMENT                              "BlpI: 2 sites") 2 5 "GCTNAGC" "CGANTCG") 
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COMMENT                              (CGroupPar (CParagraph 63713576 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 62752896 (8 0) 1 1 1 0 178) 
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COMMENT                             "ACTGGGNNNNNN" "TGACCCNNNNNN") 
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COMMENT                              (CGroupPar (CParagraph 62749600 (8 0) 1 1 1 0 178) 
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COMMENT                             "NNNNNNNNNNNNNNGAGNNNNNCTCNNNNNNNNNNNNNN" 
COMMENT                             "NNNNNNNNNNNNNNCTCNNNNNGAGNNNNNNNNNNNNNN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62749536 (8 0) 1 1 1 0 178) 
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COMMENT                             "CTGGAGNNNNNNNNNNNNNNNNN" "GACCTCNNNNNNNNNNNNNNNNN") 
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COMMENT                                              191) 
COMMENT                                             " N1: 181 (on complementary strand)" 
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COMMENT                                              191) " N2: 1254 " 1))))) 
COMMENT                              "Bpu10I: 2 sites") 2 5 "CCTNAGC" "GGANTCG") 
COMMENT                       #472=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752224 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BpuEI: 10 sites") 22 20 
COMMENT                             "CTTGAGNNNNNNNNNNNNNNNNN" "GAACTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #473=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752032 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaAI: 7 sites") 3 3 "YACGTR" 
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COMMENT                              (CGroupPar (CParagraph 62751968 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 62751840 (8 0) 1 1 1 0 178) 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751776 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaI: 3 sites") 7 11 
COMMENT                             "GGTCTCNNNNNN" "CCAGAGNNNNNN") 
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COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751712 (8 0) 1 1 1 0 178) 
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COMMENT                               (CObjectList)) "BsaWI: 7 sites") 1 5 "WCCGGW" 
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COMMENT                       #479=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751520 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsaXI: 4 sites") 21 18 
COMMENT                             "ACNNNNNCTCCNNNNNNNNNNN" "TGNNNNNGAGGNNNNNNNNNNN") 
COMMENT                       #480=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751392 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BscAI: 16 sites") 9 11 
COMMENT                             "GCATCNNNNNNN" "CGTAGNNNNNNN") 
COMMENT                       #481=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754400 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseMII: 13 sites") 15 13 
COMMENT                             "CTCAGNNNNNNNNNNN" "GAGTCNNNNNNNNNNN") 
COMMENT                       #482=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751136 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BseRI: 5 sites") 16 14 
COMMENT                             "GAGGAGNNNNNNNNNNN" "CTCCTCNNNNNNNNNNN") 
COMMENT                       #483=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754112 (8 0) 1 1 1 0 178) 
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COMMENT                             "GGGTCG") 
COMMENT                       #484=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753856 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsgI: 4 sites") 22 20 
COMMENT                             "GTGCAGNNNNNNNNNNNNNNNNN" "CACGTCNNNNNNNNNNNNNNNNN") 
COMMENT                       #485=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753728 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiEI: 17 sites") 4 2 "CGRYCG" 
COMMENT                             "GCYRGC") 
COMMENT                       #486=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753600 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsiHKAI: 9 sites") 5 1 "GWGCWC" 
COMMENT                             "CWCGWG") 
COMMENT                       #487=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753536 (8 0) 1 1 1 0 178) 
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COMMENT                             "GCATGC") 
COMMENT                       #488=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753472 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BslI: 40 sites") 7 4 
COMMENT                             "CCNNNNNNNGG" "GGNNNNNNNCC") 
COMMENT                       #489=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753408 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmAI: 10 sites") 6 10 
COMMENT                             "GTCTCNNNNNN" "CAGAGNNNNNN") 
COMMENT                       #490=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753344 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmBI: 1 site") 7 11 
COMMENT                             "CGTCTCNNNNNN" "GCAGAGNNNNNN") 
COMMENT                       #491=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753280 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BsmFI: 14 sites") 15 19 
COMMENT                             "GGGACNNNNNNNNNNNNNNN" "CCCTGNNNNNNNNNNNNNNN") 
COMMENT                       #492=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753216 (8 0) 1 1 1 0 178) 
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COMMENT                             "CTTACGNN") 
COMMENT                       #493=(CRSFolderPar 
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COMMENT                              (CGroupPar (CParagraph 63713616 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsp1286I: 23 sites") 5 1 "GDGCHC" 
COMMENT                             "CHCGDG") 
COMMENT                       #494=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 63713656 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsp24I: 6 sites") 24 19 
COMMENT                             "GACNNNNNNTGGNNNNNNNNNNNNN" 
COMMENT                             "CTGNNNNNNACCNNNNNNNNNNNNN") 
COMMENT                       #495=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752384 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BspCNI: 13 sites") 14 12 
COMMENT                             "CTCAGNNNNNNNNNN" "GAGTCNNNNNNNNNN") 
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COMMENT                              (CGroupPar (CParagraph 62752320 (8 0) 1 1 1 0 178) 
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COMMENT                             "AGGCCT") 
COMMENT                       #497=(CRSFolderPar 
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COMMENT                               (CObjectList 
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COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 5999 " 1) 
COMMENT                                       #500=(CLinePar 
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COMMENT                                              191) " N2: 7007 " 1))))) 
COMMENT                              "BspHI: 2 sites") 1 5 "TCATGA" "AGTACT") 
COMMENT                       #501=(CRSFolderPar 
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COMMENT                             "ACCTGCNNNNNNNNN" "TGGACGNNNNNNNNN") 
COMMENT                       #502=(CRSFolderPar 
COMMENT                             (CFolderPar 
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COMMENT                              (CGroupPar (CParagraph 62750752 (8 0) 1 1 1 0 178) 
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COMMENT                             "CGTTACNNN") 
COMMENT                       #504=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62750688 (8 0) 1 1 1 0 178) 
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COMMENT                             "YGGCCR") 
COMMENT                       #505=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62750624 (8 0) 1 1 1 0 178) 
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COMMENT                       #506=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752832 (8 0) 1 1 1 0 178) 
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COMMENT                             "TGACCNN") 
COMMENT                       #507=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752768 (8 0) 1 1 1 0 178) 
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COMMENT                       #508=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752704 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 62749248 (8 0) 1 1 1 0 178) 
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COMMENT                             "GCANNNNNTGC" "CGTNNNNNACG") 
COMMENT                       #510=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62749184 (8 0) 1 1 1 0 178) 
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COMMENT                              (CGroupPar (CParagraph 62749120 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList 
COMMENT                                #512=(CGroupPar 
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COMMENT                                      (CObjectList 
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COMMENT                                              191) " N1: 2155 " 1) 
COMMENT                                       #514=(CLinePar 
COMMENT                                             (CParagraph 0 (1 #91# 2) 1 2 2 0 
COMMENT                                              191) " N2: 2278 " 1))))) 
COMMENT                              "BstEII: 2 sites") 1 6 "GGTNACC" "CCANTGG") 
COMMENT                       #515=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754240 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstF5I: 11 sites") 7 5 "GGATGNNN" 
COMMENT                             "CCTACNNN") 
COMMENT                       #516=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62753664 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstKTI: 29 sites") 3 1 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #517=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751232 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstNI: 20 sites") 2 3 "CCWGG" 
COMMENT                             "GGWCC") 
COMMENT                       #518=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751168 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstUI: 41 sites") 2 2 "CGCG" 
COMMENT                             "GCGC") 
COMMENT                       #519=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62756160 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstXI: 1 site") 8 4 
COMMENT                             "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                       #520=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62755648 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstYI: 12 sites") 1 5 "RGATCY" 
COMMENT                             "YCTAGR") 
COMMENT                       #521=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62755328 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BstZ17I: 1 site") 3 3 "GTATAC" 
COMMENT                             "CATATG") 
COMMENT                       #522=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754880 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Bsu36I: 0 sites") 2 5 "CCTNAGG" 
COMMENT                             "GGANTCC") 
COMMENT                       #523=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62757824 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtgI: 9 sites") 1 5 "CCRYGG" 
COMMENT                             "GGYRCC") 
COMMENT                       #524=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62756224 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BthCI: 76 sites") 4 1 "GCNGC" 
COMMENT                             "CGNCG") 
COMMENT                       #525=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62757056 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "BtsI: 5 sites") 8 6 "GCAGTGNNN" 
COMMENT                             "CGTCACNNN") 
COMMENT                       #526=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62756928 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Cac8I: 50 sites") 3 3 "GCNNGC" 
COMMENT                             "CGNNCG") 
COMMENT                       #527=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62757312 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CdiI: 11 sites") 4 4 "CATCG" 
COMMENT                             "GTAGC") 
COMMENT                       #528=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62757184 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "ChaI: 29 sites") 5 1 "NGATCN" 
COMMENT                             "NCTAGN") 
COMMENT                       #529=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754752 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjeI: 19 sites") 26 20 
COMMENT                             "CCANNNNNNGTNNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNCANNNNNNNNNNNNNNNN") 
COMMENT                       #530=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62752160 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CjePI: 14 sites") 26 20 
COMMENT                             "CCANNNNNNNTCNNNNNNNNNNNNNNN" 
COMMENT                             "GGTNNNNNNNAGNNNNNNNNNNNNNNN") 
COMMENT                       #531=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62751424 (8 0) 1 1 1 0 178) 
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COMMENT                             "TAGCTA") 
COMMENT                       #532=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62750144 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "Csp6I: 13 sites") 1 3 "GTAC" 
COMMENT                             "CATG") 
COMMENT                       #533=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62750848 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviAII: 25 sites") 1 3 "CATG" 
COMMENT                             "GTAC") 
COMMENT                       #534=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62749888 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "CviJI: 159 sites") 2 2 "RGCY" 
COMMENT                             "YCGR") 
COMMENT                       #535=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62749760 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DdeI: 15 sites") 1 4 "CTNAG" 
COMMENT                             "GANTC") 
COMMENT                       #536=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62749376 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DpnI: 29 sites") 2 2 "GATC" 
COMMENT                             "CTAG") 
COMMENT                       #537=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754592 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DraI: 5 sites") 3 3 "TTTAAA" 
COMMENT                             "AAATTT") 
COMMENT                       #538=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754528 (8 0) 1 1 1 0 178) 
COMMENT                               (CObjectList)) "DraIII: 4 sites") 6 3 "CACNNNGTG" 
COMMENT                             "GTGNNNCAC") 
COMMENT                       #539=(CRSFolderPar 
COMMENT                             (CFolderPar 
COMMENT                              (CGroupPar (CParagraph 62754464 (8 0) 1 1 1 0 178) 
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COMMENT                              "FalI: 2 sites") 38 1 
COMMENT                             "NNNNNNNNNNNNNNAAGNNNNNCTTNNNNNNNNNNNNNN" 
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COMMENT                               (CObjectList)) "FauI: 22 sites") 9 11 
COMMENT                             "CCCGCNNNNNNN" "GGGCGNNNNNNN") 
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COMMENT                             "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
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COMMENT                              "FseI: 2 sites") 6 2 "GGCCGGCC" "CCGGCCGG") 
COMMENT                       #571=(CRSFolderPar 
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COMMENT                             "GAYNNNNNVTCNNNNNNNNNNNNNN" 
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COMMENT                             "CCTCNNNNNNNN" "GGAGNNNNNNNN") 
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COMMENT                              "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT") 
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FEATURES             Location/Qualifiers
     Promoter        386..1529
                     /vntifkey="21"
                     /label=hEF1a\promoter
                     /note="Unknown feature type:Promoter"
     Intron          582..1520
                     /vntifkey="21"
                     /label=intron\hEF1a\promoter
                     /note="Unknown feature type:Intron"
     Promoter        1546..1562
                     /vntifkey="21"
                     /label=T7\promoter
                     /note="Unknown feature type:Promoter"
     CDS             1615..1680
                     /vntifkey="4"
                     /label=P2A
     Terminator      3067..3291
                     /vntifkey="21"
                     /label=BGH\polyA
                     /note="Unknown feature type:Terminator"
     Replication_ori 3337..3765
                     /vntifkey="21"
                     /label=f1\ori
                     /note="Unknown feature type:Replication_ori"
     Promoter        3770..4051
                     /vntifkey="21"
                     /label=SV40\early\promoter
                     /note="Unknown feature type:Promoter"
     Replication_ori 4008..4083
                     /vntifkey="21"
                     /label=SV40\ori
                     /note="Unknown feature type:Replication_ori"
     Promoter        4156..4221
                     /vntifkey="21"
                     /label=EM7\promoter
                     /note="Unknown feature type:Promoter"
     CDS             4222..4620
                     /vntifkey="4"
                     /label=BSD
     Terminator      4778..4852
                     /vntifkey="21"
                     /label=SV40\polyA\early
                     /note="Unknown feature type:Terminator"
     Terminator      complement(4778..4852)
                     /vntifkey="21"
                     /label=SV40\polyA\late
                     /note="Unknown feature type:Terminator"
     Replication_ori complement(5166..5557)
                     /vntifkey="21"
                     /label=pMB1\ori
                     /note="Unknown feature type:Replication_ori"
     CDS             complement(6098..6958)
                     /vntifkey="4"
                     /label=AMP
     CDS             1696..3042
                     /vntifkey="4"
                     /label=mcrA
                     /note="Mitomycin C resistance"
BASE COUNT     1487 a      1942 c      2016 g      1649 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtggaagc 
     1621 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct 
     1681 cacagtggcg gccgcatgag cacgcaatgg ggatgggccc ttgagccgga ccagccggga 
     1741 tacgacgacg cccggctggg actgaaccgg gcggccgaat cgcggccggc ctacgtggtc 
     1801 gaggcggccg acgagcagga ggtggccgcc gcggtgaggc tggccgccga gcagaaacgg 
     1861 cccgtgggtg tgatggccac cggtcacgga ccgtccgtgt cggccgacga cgccgtgctg 
     1921 gtcaacacgc ggcggatgga aggtgtgagc gttgacgcgg cccgcgcgac ggcatggatc 
     1981 gaagccgggg cacgctggcg gaaggtgctg gaacacaccg ctccgcacgg gctcgcgccg 
     2041 ctgaacggct ctagccccaa cgtgggcgct gtcggctatc tggtcggcgg cggcgcggga 
     2101 ctgctgggcc gccggttcgg ctacgccgcc gaccacgtac ggcggctgcg cctggtcacc 
     2161 gccgacggcc gcttgcgcga cgtgacggcc gggaccgacc ccgacctgtt ctgggcggtc 
     2221 cgcggcggca aggacaactt cggcctggtc gtgggcatgg aggtcgacct gttcccggtc 
     2281 acccggctct acggcggagg gctctacttc gcgggcgagg ccaccgccga ggtgctgcac 
     2341 gcctacgccg agtgggtccg gcacgtgccc gaggagatgg cgtcctccgt gctgctcgtc 
     2401 cacaaccccg acctgcccga cgtcccggaa ccgctgcgcg gacgcttcat cacccacctc 
     2461 cgcatcgcct acagcggcga accggcagac ggcgagcact tggtgcgacc gctacgcgaa 
     2521 ctcggaccca tcctcctcga caccgtgcgg gacatgccct acgccgaggt cggcacgatt 
     2581 catcacgagc ccacgtccat gccgtacgtc gcgtacgacc gcaacgtgtt gctgagcgac 
     2641 ctgaccgacg atgccgtcga catcatcgtc gccctggccg gaccggacgc aggggcgccg 
     2701 ttcgtcaccg aactgcggca cttcggcggc gcgtacgccc gtccgccgaa ggtccccaac 
     2761 tgcgtgggcg ggcgcgacgc ggccttctcg ctcttcacgg gcgccgtccc ggaagccgag 
     2821 ggtctccggc gccgtgatga cctgctcgac cggctgcgcc catggagcac cggcggcacg 
     2881 aacctcaatt tcgccggtgt cgaggacatc agcccggcga gcgtggaagc cgcctacact 
     2941 ccggctgatt tcgcccggtt gagggctgtc aaggcccaat acgacccgga caacatgttc 
     3001 cgagtcaact tcaacattcc gccggcggag tcttggacgt gagataaacc cgctgatcag 
     3061 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     3121 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     3181 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     3241 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 
     3301 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 
     3361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     3421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     3481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     3541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     3601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     3661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     3721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 
     3781 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     3841 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 
     3901 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     3961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     4021 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 
     4081 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 
     4141 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac 
     4201 gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt 
     4261 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc 
     4321 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg 
     4381 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg 
     4441 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc 
     4501 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga 
     4561 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa 
     4621 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc 
     4681 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 
     4741 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 
     4801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     4861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 
     4921 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     4981 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 
     5041 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     5101 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     5161 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     5221 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     5281 aggaaagaac atgtgagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     5341 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     5401 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     5461 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     5521 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     5581 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     5641 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     5701 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     5761 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     5821 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     5881 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     5941 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     6001 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     6061 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     6121 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     6181 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     6241 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     6301 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     6361 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     6421 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     6481 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     6541 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     6601 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     6661 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     6721 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     6781 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     6841 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     6901 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     6961 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     7021 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     7081 tgccacctga cgtc 
//

