LOCUS       pTrc99A-CsCDP        6513 bp ds-DNA     circular SYN 06-DEC-2016
DEFINITION  LMBP 10211.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     VNTDATE|788486400|
COMMENT     VNTNAME|pTrc99A-CsCDP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_feature    193..222
                     /label=trc promoter
     misc_feature    267..2639
                     /label=CDP
     misc_feature    3183..4043
                     /label=bla
                     /note="beta-lactamase"
     misc_feature    5392..6474
                     /label=lacIq
                     /note="lac repressor protein"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgc accatcacca tcaccatcgt tacggttatt
      301 ttgatgaaaa agcccgcgaa tatgttatta caagacccga tactccgaca ccatggataa
      361 actacatagg caacgggaaa tacggaggca tagttacaaa caccggaggc ggctacagtt
      421 ttcacaagga cccacagaac agaagaatta caagatacag atacaacaat ctccccaccg
      481 acaggccggg caggtatatt tacgtccgcg acaggctgac gggcgaatac tggaatcccg
      541 gctatcagcc cgtgcagaga aaactcgact cttacaggtg caggcatgga atgggttata
      601 ctgttctcga aggggagtac aagggcattg cagccgatgt gacctatttt gtacccgatg
      661 acagggactt tgaaatctgg cttgtacaaa tcagaaacct ctgccatgtt gaaagaaacc
      721 ttcaggtctt cagttacgcc gaattctgtt tctgggatgc cataatggat cagcagaatg
      781 tggactgggt tcagcagata aaccagggaa ggtatgaaga caggcttata acgtggcatc
      841 cgcatcattt taaggacgca tgcgcattct ttgccacaaa tgccgaaata aacagttttg
      901 ataccaatct tgaggcgttt atcggaagat accgctgcga atctaacccc atcgccgtgg
      961 aaacaggggc atgttccaat tccgtgtctt acagaatgaa cggtgtcggt gcgttttgca
     1021 tcgacgtaaa tttaaaaccg ggggaagagc gggaaattat atttattttg ggctttaccg
     1081 aaaacaagag tacaataagg gacgaaatcc gcgattacct taacgtggaa tatgcaaaag
     1141 aagctctaaa aaggctaaag gactcctggg aggaatatct ggacaaactg cagattgaaa
     1201 cccccgatcg ggaaaccaat ctctttgtta acacatggaa tcagtatcag tgcaaaataa
     1261 ccttcaactg gtcaagattt gtatcgatgt accagctggg gctgggaagg ggaataggga
     1321 tcagggacag cgcccaggac acgctcggcg ttatgcattc aatacccgag ctggcaggcg
     1381 ggcttattaa acggttaatt cactgccagt ataccgacgg gcgtgtttat catctgtttt
     1441 tccctttgac aggcgaaggc gggatcggtg acgccccagt tgtgaaattt gactggtatt
     1501 ccgatgatca tctgtggctg cccatcgccg caaacgcata tctgaaggaa acggcgaatt
     1561 tcgatttttt ggaaagtgtt gtgccgtata acgacaataa aaccgaaggt accgtatggg
     1621 agcatctcaa cagggccatg gaattcacat acaaccaccg cggaccgcac ggccttgccc
     1681 ttataggcag agccgactgg aacgataccc tgaacctgga tatgggaaac ggaatagccg
     1741 aaagtgtttt tacttcaatg cttttctgca gagccgccct tgaaatgatt cagatggccg
     1801 aatataccgg acaaaaggat ttggcgacaa aatacaggta ttggtatgat gaaatgaaaa
     1861 aagccataaa tgaatggtgc tgggacggag aatggtacat aagggcattt gacgatgagg
     1921 gtaatgtcct tggttcgggt aaaaacagat acgggaaaat tttcattaac agccagtcat
     1981 gggcggttct tagcatggtt gcgcccgaag aatacgctaa gaaatgcctc gagtcggttt
     2041 atcggcacct gaacacgaag tacgggatag ttaaagtata tccggcatat ccggaatata
     2101 atccgaaaat agggggcatg accacatacc cgccgggggc gaaggaaaac ggcggtattt
     2161 ttgcccatac caacccatgg gtaatgattg ccgaatgtat gatgggcaac ggaaggcggg
     2221 cttatcagta ctacaggcag atactcccgt taacgcgcaa tgacgacgcc gatttgcttg
     2281 aagtggaacc ttacgtgtac tgccagaata tactcggaaa agagcatccg cagttcggaa
     2341 taggccgtaa ttcgtggctt acaggcactg cagcatggaa tatggttgcg gtaagccagt
     2401 atattttggg aataagaccg gaatacgacg gcctgacggt ggatccgtgc attccgcctg
     2461 actggaaagg ttttaaagtg agaagaattt tccggggttg tgtttacaat atcgaagtaa
     2521 gaaatcctga aggcgtaagc agaggtgtga aaaaaattgt cgtagacgga gttgaaaccg
     2581 acaaaatacc cgtaaaacct gcaggaacgg tttgtgaatg tgttgttata atgggatgag
     2641 tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
     2701 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     2761 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     2821 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     2881 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2941 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     3001 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     3061 gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata
     3121 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3181 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3241 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3301 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3361 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3421 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3481 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3541 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3601 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3661 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3721 tgcctacagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3781 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3841 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3901 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3961 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4021 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4321 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4561 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4621 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4681 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4741 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4801 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4861 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4921 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4981 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     5041 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     5101 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     5161 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5221 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc
     5281 gcgcgcgaag gcgaagcggc atgcatttac gttgacacca tcgaatggtg caaaaccttt
     5341 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     5401 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     5461 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     5521 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     5581 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     5641 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     5701 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     5761 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     5821 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     5881 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     5941 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     6001 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     6061 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     6121 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     6181 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     6241 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     6301 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     6361 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6421 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6481 cgcaattaat gtgagttagc gcgaattgat ctg
//
