LOCUS       pET-21a-PhF3GalT-sumo        7083 bp ds-DNA     circular SYN 06-DEC-2016
DEFINITION  LMBP 10220.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Vector molecule:  pET21a-H6-SUMO cut XhoI-NcoI
               Fragment ends: XhoI and NcoI
               Fragment size: 5725
            Insert molecule:  GalT_S2A cut NcoI-XhoI
               Fragment ends: NcoI and XhoI
               Fragment size: 1358
COMMENT     VNTDATE|788486400|
COMMENT     VNTNAME|pET-21a-PhF3GalT-sumo|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_feature    4..363
                     /label=H6-SUMO
     CDS             4
                     /product="epitope tag from a T7 major capsid protein"
                     /label=epitope'
                     /note="T7 tag"
                     /note="epitope tag from a T7 major capsid protein"
     misc_feature    364..1720
                     /label=F3GalT
     misc_feature    complement(1721..1725)
                     /label=MCSmultiple''
                     /note="MCS"
                     /note="multiple cloning site"
                     /note="MCSmultiple cloning site"
     CDS             1726..1743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="6xHis"
                     /note="6xHis affinity tag"
     CDS             2481..3341
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="beta-lactamase"
     CDS             complement(4530..4721)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /note="rop"
                     /note="Rop protein"
     CDS             complement(5530..6612)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="lac repressor"
ORIGIN
        1 catatgggca gcagccatca tcatcatcat cacggcagcg gcctggtgcc gcgcggcagc
       61 gctagcatgt cggactcaga agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc
      121 aagcctgaga ctcacatcaa tttaaaggtg tccgatggat cttcagagat cttcttcaag
      181 atcaaaaaga ccactccttt aagaaggctg atggaagcgt tcgctaaaag acagggtaag
      241 gaaatggact ccttaagatt cttgtacgac ggtattagaa ttcaagctga tcagacccct
      301 gaagatttgg acatggagga taacgatatt attgaggctc acagagaaca gattggtggt
      361 gccatggcta actatcatgt tgcggtcctg gcctttccgt tcgcaaccca cgctggtctg
      421 ctgctgggtc tggtccagcg tctggctaac gcgctgccga atgtgacctt tacgtttttc
      481 aacacgtcaa aatcgaatag ctctctgttc accacgccgc atgataacaa catcaaaccg
      541 ttcaacatca gcgacggcgt gccggaaggt tacgtggttg gcaaaggcgg tattgaagca
      601 ctgatcggtc tgtttttcaa atctgcgaaa gaaaacattc agaatgcgat ggcggccgca
      661 gttgaagaaa gtggcaagaa aattacctgc gtcatggccg atgcatttat gtggttctcc
      721 ggtgaaattg ccgaagaact gtcagtgggc tggatcccgc tgtggacctc tgctgcgggt
      781 agcctgtctg tgcatgttta tacggatctg attcgtgaaa acgttgaagc acaaggcatc
      841 gctggtcgcg aagacgaaat tctgaccttt atcccgggtt tcgcggaact gcgtctgggc
      901 agtctgccgt ccggtgtcgt gagtggcgat ctggaatcac cgttctcggt gatgctgcac
      961 aaaatgggta aaaccattgg caaagctacg gcgctgccgg tgaattcgtt tgaagaactg
     1021 gatccgccga tcgttgaaga cctgaaaagc aaattcaaca atttcctgaa cgttggtccg
     1081 ttcaatctga ccaccccgcc gccgtcggca aacattaccg atgaatatgg ttgtatcgca
     1141 tggctggaca aacaggaacc gggcagcgtg gcatacattg gctttggtac cgttgctacc
     1201 ccgccgccga atgaactgaa agcgatggca gaagctctgg aagaatcaaa aaccccgttt
     1261 ctgtggtcgc tgaaagacct gtttaaaagc tttttcccgg aaggttttct ggaacgcacg
     1321 tctgaatatg gcaaaattgt gagttgggcg ccgcaggtcc aagtgctgag tcatggctct
     1381 gttggtgtct ttatcaacca ctgcggttgg aatagtgttc tggaatccat tgccgcaggt
     1441 gtgccggtta tctgtcgtcc gtttttcggc gatcatcaac tgaacgcgtg gatggtcgaa
     1501 aaagtgtgga aaattggtgt taaaatcgaa ggcggtgtct tcaccaaaga cggcacgatg
     1561 ctggccctgg atctggtgct gagcaaagac aaacgcaata ccgaactgaa acagcaaatt
     1621 ggcatgtaca aagaactggc actgaacgca gtcggtccgt ccggtagttc cgcagaaaac
     1681 ttcaaaaaac tggttgatat catcacctca tgcaactaac tcgagcacca ccaccaccac
     1741 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     1801 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     1861 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     1921 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1981 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2041 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2101 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2161 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2221 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2281 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     2341 ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2401 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2461 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2521 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2581 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2641 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2701 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2761 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2821 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2881 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2941 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3001 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     3061 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3121 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3181 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3241 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3301 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3361 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3421 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3841 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3901 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3961 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4021 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4081 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4141 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4201 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4261 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     4321 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4381 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     4441 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4501 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4561 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     4621 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     4681 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     4741 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     4801 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     4861 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     4921 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     4981 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     5041 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     5101 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     5161 gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc
     5221 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
     5281 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
     5341 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
     5401 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
     5461 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
     5521 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5581 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     5641 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     5701 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     5761 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
     5821 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     5881 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     5941 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     6001 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     6061 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     6121 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     6181 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     6241 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     6301 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     6361 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     6421 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     6481 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     6541 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     6601 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
     6661 gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact
     6721 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     6781 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     6841 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     6901 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     6961 gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga
     7021 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
     7081 ata
//
