LOCUS       pTrc99A-CuCP            6627 bp ds-DNA     circular SYN 06-DEC-2016
DEFINITION  LMBP 10222.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Vector molecule:  pTrc99a_cut
               Fragment ends: blunt
               Fragment size: 4140
            Insert molecule:  CuCP_gene
               Fragment ends: blunt
               Fragment size: 2511
COMMENT     VNTDATE|788486400|
COMMENT     VNTNAME|pTrc99A-CuCP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_feature    193..222
                     /label=trc promoter
     misc_feature    193..198
                     /label=trp
     misc_feature    216..222
                     /label=lacUV5
     misc_feature    265..266
                     /label=NcoI site'
     misc_feature    267..2753
                     /label=CuCP
                     /note="Cellulomonas uda Cellobiose Phosphorylase"
     misc_feature    2983..3026
                     /label=rrnB T1
     misc_feature    3158..3185
                     /label=rrnB T2
     misc_feature    3297..4157
                     /label=bla
                     /note="beta-lactamase"
     misc_feature    4915
                     /label=ori
     misc_feature    5506..6588
                     /label=lacIq
                     /note="lac repressor protein"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgc accaccatca ccatcatcgc tatggccatt
      301 ttgacgatga ggcacgtgaa tatgtgatca cgaccccaca tacgccgtac ccgtggatca
      361 actatctggg cagtgagcag tttttttcgc tgctgagcca ccaagccggt ggttatagct
      421 tttaccgcga tgcaaagatg cgtcggttga cccgctatcg ctataataac atccctgcgg
      481 atgccggagg ccgctatctg tacgttaatg atggtggtga tgtctggaca ccaagctggc
      541 tgccggtcaa ggcggatttg gatcactttg aggcccgcca cggtctgggc tattcaacca
      601 ttacaggcga acggaatggt gttcgcgtcg aaacgctgtt ctttgttcca gtgggggaaa
      661 atgccgaagt gcagaaagta acggtcacca atacctcgga tagctataaa tctttgaccc
      721 tgttttcctt cgtggagttt tgcttatgga acgcccaaga tgatcagact aactaccaac
      781 gcaacctgag tatcggcgaa gtggaggtag aacaggagag tcctcatggc agcgcaatct
      841 atcaccgtac agaatatcgt gaacgccgcg atcactatgc agttttcgcc gtgaataccc
      901 aggccgaagg attcgacact gatcgcgaca ccttcgtcgg cgcctataac tcactgggag
      961 aggctgccgt cccgctgaag ggtgaaagcg caaactcagt tgcatcaggt tggtatccga
     1021 ttggaagtca ctcggttgca gtttccttag cgccgggcga aagtcgtgag cttgtgtacg
     1081 tgctgggcta tgtggaaaac ccggacgagg aaaagtgggc ggatgacgca aagcaggtag
     1141 tgaataaaga gcgtgcccat gcgctgctct cccgctttgc tacgtccgaa cagactgatg
     1201 cggcatttgc cgccctgaaa gactactgga ccgatttatt aagcacttat tcagtcagtt
     1261 caaacgacga aaagttggat cgtatggtta atatttggaa ccagtaccag tgtatggtca
     1321 cgtttaacat gagtcggtca gcgtctttct tcgaaacggg catcggtcgc gggatgggtt
     1381 tccgtgattc taatcaagac ctgttaggtt ttgtacatct tattccagaa cgggcacggg
     1441 aacggattat cgatattgca tcaacccagt ttgcggacgg ctccgcatat caccagtacc
     1501 agccgctgac taaacgtggc aataacgaca ttggatccgg tttcaacgat gatccattat
     1561 ggcttatcgc tggcactgcg gcttatatta aagaaacggg agatttttcc attctggacg
     1621 agccagtccc atttgacaat gagccaggta gtgaagttcc gttattcgaa catttgactc
     1681 gctcgttcga gttcaccgtc acgcaccgtg gaccgcatgg cctgccactg atcggtcggg
     1741 cggattggaa tgattgtctg aacctgaact gcttttccac cacgccggga gaaagctttc
     1801 aaaccaccga aaatcaggcc ggcggtgtcg ccgaatcaac gttcatcgct gcacagtttg
     1861 tgctctatgg ggagcagtat gccgaactgg cagctcggcg gggtttggcc gatgtggcgg
     1921 atcgggcgcg tggtcatgtg gctgaaatgc gtgatgcact gctgaccgac ggctgggacg
     1981 gctcttggtt cctgcgcgct tacgattatt acggcaaccc gatcggtacg gatgcccacg
     2041 atgagggcaa aatctggatc gagccccagg gattcgcggt tatggctggg gttggtgtcg
     2101 gcgaaggacc gcaggatacg gacgctccgg ccatcaaagc gttggattcc gtgaatgaga
     2161 tgctcgcgac cgatcatggt atggttctgc aataccctgc gtatactacc tatcaagtcc
     2221 acatgggcga agtatctacg tacccgcctg gctacaaaga gaacggtggt atcttttgtc
     2281 ataataaccc ttgggttatt attgcggaaa cagtggttgg tcgtggtgga cgtgccttcg
     2341 attattacaa acgcattacc ccagcgtatc gcgaagacat ctcggacgtg caccgccttg
     2401 aaccgtatgt atacgctcag atgattgcag ggaaggaggc agtgcgccac ggcgaagcta
     2461 agaactcatg gttgaccggc actgccgctt ggaactttgt gacggtttcc caatatctgt
     2521 taggcgtgcg ccccgaatat gacggcctcg tcgttgatcc tcaaattggg cctgatgttc
     2581 caagcttcac cgtgactcgt gtcgcccgcg gggctaccta tgaaattaca gtaaccaatt
     2641 caggcacgga cgggtcacgt ggccgtctgg ttgtggacgg taccccggta gaaggaaatc
     2701 tcgttcctta tgcaccagcc ggatcgaccg ttcgcgttga tgttaccctt taagtcgacc
     2761 tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga
     2821 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg
     2881 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
     2941 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
     3001 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     3061 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
     3121 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc
     3181 ctttttgcgt ttctacaaac tctttttgtt tatttttcta aatacattca aatatgtatc
     3241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     3541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgccta
     3841 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5101 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     5161 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     5221 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     5281 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5341 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc
     5401 gaaggcgaag cggcatgcat ttacgttgac accatcgaat ggtgcaaaac ctttcgcggt
     5461 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg
     5521 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
     5581 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
     5641 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
     5701 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
     5761 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
     5821 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
     5881 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
     5941 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
     6001 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
     6061 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
     6121 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
     6181 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
     6241 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
     6301 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     6361 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     6421 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     6481 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6541 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6601 taatgtgagt tagcgcgaat tgatctg
//
