LOCUS       pTrc99A-CtCP            6609 bp ds-DNA     circular SYN 06-DEC-2016
DEFINITION  LMBP 10224.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Vector molecule:  pTrc99a
               Fragment ends: blunt
               Fragment size: 4140
            Insert molecule:  CtCP
               Fragment ends: blunt
               Fragment size: 2454
COMMENT     VNTDATE|788486400|
COMMENT     VNTNAME|pTrc99A-CtCP|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_feature    193..222
                     /label=trc promoter
     misc_feature    193..198
                     /label=trp
     misc_feature    216..222
                     /label=lacUV5
     misc_feature    265..266
                     /label=NcoI site'
     misc_feature    282..2735
                     /label=CtCP
     misc_feature    2965..3008
                     /label=rrnB T1
     misc_feature    3140..3167
                     /label=rrnB T2
     misc_feature    3279..4139
                     /label=bla
                     /note="beta-lactamase"
     misc_feature    4897
                     /label=ori
     misc_feature    5488..6570
                     /label=lacIq
                     /note="lac repressor protein"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg aattcgagct catgcatcat catcatcacc
      301 ataaatttgg cttttttgat gatgccaata aagaatatgt gattaccgtt ccgcgtaccc
      361 cgtatccgtg gattaattat ctgggcaccg aaaatttttt tagcctgatt agcaataccg
      421 caggcggtta ttgtttttat cgtgatgcac gtctgcgtcg tattacccgt tatcgttata
      481 ataatgtgcc gattgatatg ggtggtcgct atttttatat ttatgataat ggcgattttt
      541 ggagtccggg ttggagtccg gttaaacgtg aactggaaag ctatgaatgt cgtcatggtc
      601 tgggctatac caaaattgca ggtaaacgca atggcattaa agccgaagtt accttttttg
      661 tgccgctgaa ttataacggc gaagtgcaga aactgattct gaaaaatgaa ggccaggata
      721 aaaaaaaaat taccctgttt agctttattg aattttgcct gtggaatgcc tatgatgata
      781 tgaccaattt tcagcgcaat tttagcaccg gtgaagttga aattgaaggc agcgtgattt
      841 atcataaaac cgaatatcgc gaacgtcgca atcattatgc cttttatagc gtgaatgcca
      901 aaatttcagg ctttgatagc gatcgcgata gctttattgg tctgtataat ggttttgatg
      961 caccgcaggc agttgttaat ggcaaaagca ataatagcgt tgcagatggt tgggcaccga
     1021 ttgcaagcca tagcattgaa attgaactga atccgggtga acagaaagaa tatgttttta
     1081 ttattggcta tgtggaaaat aaagatgaag aaaaatggga aagcaaaggc gtgattaata
     1141 aaaaaaaagc ctatgaaatg attgaacagt ttaataccgt ggaaaaagtg gataaagcct
     1201 ttgaagaact gaaaagctat tggaatgccc tgctgagcaa atattttctg gaaagccatg
     1261 atgaaaaact gaatcgcatg gtgaatattt ggaatcagta tcagtgcatg gtgaccttta
     1321 atatgagccg tagcgcaagc tattttgaaa gcggtattgg tcgtggtatg ggttttcgtg
     1381 atagcaatca ggatctgctg ggttttgttc atcagattcc ggaacgtgca cgtgaacgtc
     1441 tgctggatct ggcagcaacc cagctggaag atggtagcgc atatcatcag tatcagccgc
     1501 tgaccaaaaa aggcaataat gaaattggca gcaattttaa tgatgatccg ctgtggctga
     1561 ttctggcaac cgcagcatat attaaagaaa ccggtgatta tagcattctg aaagaacagg
     1621 tgccgtttaa taatgatccg agcaaagcag ataccatgtt tgaacatctg acccgtagct
     1681 tttatcatgt ggttaataat ctgggtccgc atggtctgcc gctgattggt cgtgcagatt
     1741 ggaatgattg tctgaatctg aattgcttta gcaccgttcc ggatgaaagc tttcagacca
     1801 ccaccagtaa agatggtaaa gttgcagaaa gcgttatgat tgccggtatg tttgtgttta
     1861 ttggcaaaga ttatgtgaaa ctgtgcgaat atatgggcct ggaagaagaa gcacgtaaag
     1921 cacagcagca tattgatgca atgaaagaag ccattctgaa atacggctat gatggtgaat
     1981 ggtttctgcg tgcatatgat gattttggtc gtaaagtggg cagcaaagaa aatgaagaag
     2041 gcaaaatttt tattgaaagc cagggctttt gcgtgatggc agaaattggt ctggaagatg
     2101 gcaaagcact gaaagccctg gatagcgtta aaaaatatct ggataccccg tatggtctgg
     2161 ttctgcagaa tccggcattt acccgttatt atattgaata tggcgaaatt tccacctatc
     2221 cgccgggtta taaagaaaat gccggtattt tttgccataa taatgcctgg attatttgtg
     2281 ccgaaaccgt tgttggtcgt ggtgatatgg cctttgatta ttatcgtaaa attgcaccgg
     2341 catatattga agatgtgagc gatattcata aactggaacc gtatgtttat gcacagatgg
     2401 ttgcaggtaa agatgcaaaa cgtcatggtg aagcaaaaaa tagctggctg accggcaccg
     2461 cagcatggaa ttttgttgca attagccagt ggattctggg tgttaaaccg gattatgatg
     2521 gcctgaaaat tgatccgtgt attccgaaag catgggatgg ttataaagtg acccgttatt
     2581 ttcgtggtag cacctatgaa atcaccgtga aaaatccgaa tcatgtgagc aaaggtgtgg
     2641 caaaaattac cgttgatggc aatgaaatta gcggtaatat tctgccggtg tttaatgatg
     2701 gcaaaaccca taaagtggaa gtgattatgg gttaagtcga cctgcaggca tgcaagcttg
     2761 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
     2821 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     2881 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     2941 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3001 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     3061 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     3121 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     3181 actctttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3301 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3361 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3421 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3481 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     3541 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3601 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3661 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3721 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3781 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tacagcaatg gcaacaacgt
     3841 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3901 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3961 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4021 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4081 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4141 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4201 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4441 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     5101 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     5161 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     5221 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5281 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5341 gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc
     5401 atttacgttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg
     5461 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
     5521 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     5581 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     5641 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     5701 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     5761 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     5821 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     5881 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     5941 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     6001 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     6061 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     6121 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     6181 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     6241 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
     6301 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
     6361 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     6421 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     6481 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6541 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagcgcga
     6601 attgatctg
//
