LOCUS       pET28-TNFR1             5893 bp    DNA     circular      8-FEB-2018
DEFINITION  LMBP 10233.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11656119|
COMMENT     VNTDBDATE|-12151532|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pET28-TNFR1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   6.96932 0 -10 11.0817 1.16519 #141#) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "@N" 1 9.70182 11.2899 0 -10 5.05742 1.16519 
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COMMENT                                   (LOGPEN 0 4 10040064) 6 1 6.1167 1.9 0.082322 
COMMENT                                   1) 
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COMMENT                            (CObjectList 
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COMMENT                                   (CLine 
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COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 5.34919 5.43645) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "T7 promoter with lac operator" "@N" 1 
COMMENT                                   17.0649 8.28326 0 -10 11.9246 1.16519 #151#) 
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FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /vntifkey="43"
                     /label=T7\terminator
     misc_marker     complement(140..157)
                     /vntifkey="22"
                     /label=His-tag
     CDS             3188..3379
                     /vntifkey="4"
                     /label=rop
     rep_origin      complement(3625..4027)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             4519..5334
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      complement(5427..5882)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        complement(869..910)
                     /vntifkey="30"
                     /label=T7\promoter\with\lac\operator
     promoter        1219..1296
                     /vntifkey="30"
                     /label=lacI\promoter,\incl.\RBS
     CDS             1297..2379
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(167..823)
                     /vntifkey="4"
                     /label=hTNF\mutant
BASE COUNT     1368 a      1532 c      1620 g      1373 t 
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagcggtcac agggcaatga 
      181 tcccaaagta gacctgccca gactcggcaa agtcgagata gtcgggccga ttgatctcag 
      241 cgctgagtcg gtcacccttc tccagctgga agacccctcc cagatagatg ggctcatacc 
      301 agggcttggc ctcagccccc tctggggtct ccctctggca ggggctcttg atggcagaga 
      361 ggaggttgac cttggtctgg taggtgacgg cgatctggta ggtgacggcg atgcggctga 
      421 tggtgtgggt gaggagcaca tgggtggagg ggcagccttg gcccttgaag aggacctggg 
      481 agtagatgag gtacaggccc tctgatggca ccaccagctg gttatctctc agctccacgc 
      541 cattggccag gagggcattg gcccgccagt tcagccactg gagctgcccc tcagcttgag 
      601 ggtttgctac aacatgggct acaggcttgt cactcggggt tcgagaagat gatctgactg 
      661 cctgggccag agggctgatt agagagaggt ccctggggaa ctcttccctc tgggggccga 
      721 tcaccggatc ccgacccatt tgctgtccac cagtcatgct agccatatgg ctgccgcgcg 
      781 gcaccaggcc gctgctgtga tgatgatgat gatggctgcc catggtatat ctccttctta 
      841 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg 
      901 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg 
      961 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg 
     1021 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag 
     1081 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg 
     1141 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg 
     1201 gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga 
     1261 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 
     1321 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 
     1381 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 
     1441 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 
     1501 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 
     1561 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 
     1621 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 
     1681 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 
     1741 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 
     1801 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 
     1861 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 
     1921 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 
     1981 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 
     2041 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 
     2101 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 
     2161 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 
     2221 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 
     2281 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 
     2341 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag 
     2401 ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt 
     2461 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 
     2521 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg 
     2581 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc 
     2641 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat 
     2701 tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg 
     2761 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac 
     2821 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg 
     2881 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg 
     2941 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa 
     3001 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg 
     3061 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag 
     3121 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga 
     3181 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca 
     3241 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg 
     3301 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga 
     3361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     3421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     3481 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 
     3541 tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg 
     3601 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     3661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     3721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     3781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     4021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     4081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     4261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     4321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4441 acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac 
     4501 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga 
     4561 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 
     4621 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg 
     4681 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 
     4741 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 
     4801 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct 
     4861 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 
     4921 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 
     4981 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 
     5041 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc 
     5101 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 
     5161 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 
     5221 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 
     5281 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta 
     5341 attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     5401 cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc 
     5461 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 
     5521 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 
     5581 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 
     5641 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 
     5701 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 
     5761 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 
     5821 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 
     5881 gtcccattcg cca 
//
