LOCUS       pET28-TNFR2             5878 bp    DNA     circular      8-FEB-2018
DEFINITION  LMBP 10233.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12151542|
COMMENT     VNTDBDATE|-12151542|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pET28-TNFR2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "@N" 1 11.4372 8.71165 0 -10 5.05742 1.16519 
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COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
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COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 5.36284 5.4501) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   "T7 promoter with lac operator" "@N" 1 
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FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /vntifkey="43"
                     /label=T7\terminator
     misc_marker     complement(140..157)
                     /vntifkey="22"
                     /label=His-tag
     CDS             3173..3364
                     /vntifkey="4"
                     /label=rop
     rep_origin      complement(3610..4012)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             4504..5319
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      complement(5412..5867)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        complement(854..895)
                     /vntifkey="30"
                     /label=T7\promoter\with\lac\operator
     promoter        1204..1281
                     /vntifkey="30"
                     /label=lacI\promoter,\incl.\RBS
     CDS             1282..2364
                     /vntifkey="4"
                     /label=lacI
     CDS             complement(167..808)
                     /vntifkey="4"
                     /label=hTNF\mutant
BASE COUNT     1366 a      1528 c      1614 g      1370 t 
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagcggtcac agggcaatga 
      181 tcccaaagta gacctgccca gactcgcgaa agttgagata gtcgggccga ttgatctcag 
      241 cgctgagtcg gtcacccttc tccagctgga agacccctcc cagatagatg ggctcatacc 
      301 agggcttggc ctcagccccc tctggggtct ccctctggca ggggctcttg atggcagaga 
      361 ggaggttgac cttggtctgg taggagacgg cgatgcggct gatggtgtgg gtgaggagca 
      421 catgggtgga ggggcagcct tggcccttga agaggacctg ggagtagatg aggtacaggc 
      481 cctctgatgg caccaccagc tggttatctc tcagctccac gccattggcc aggagggcat 
      541 tggcccggca gttcagccac tggagctgcc cctcagcttg agggtttgct acaacatggg 
      601 ctacaggctt gtcactcggg gttcgagaag atgatctgac tgcctgggcc agagggctga 
      661 ttagagagag gtccctgggg aactcttccc tctgggggcc gatcaccgga tcccgaccca 
      721 tttgctgtcc accagtcatg ctagccatat ggctgccgcg cggcaccagg ccgctgctgt 
      781 gatgatgatg atgatggctg ctgcccatgg tatatctcct tcttaaagtt aaacaaaatt 
      841 atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc 
      901 gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca 
      961 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc 
     1021 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg 
     1081 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc 
     1141 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc 
     1201 ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag 
     1261 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc 
     1321 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 
     1381 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 
     1441 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 
     1501 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 
     1561 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 
     1621 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 
     1681 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 
     1741 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 
     1801 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 
     1861 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 
     1921 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 
     1981 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 
     2041 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 
     2101 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 
     2161 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 
     2221 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 
     2281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
     2341 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta 
     2401 ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 
     2461 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 
     2521 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc 
     2581 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt 
     2641 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc 
     2701 ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg 
     2761 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg 
     2821 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta 
     2881 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg 
     2941 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc 
     3001 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg 
     3061 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt 
     3121 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa 
     3181 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct 
     3241 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga 
     3301 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 
     3361 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 
     3421 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 
     3481 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 
     3541 ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 
     3601 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     3661 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     3721 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     3781 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     3841 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     3901 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     3961 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     4021 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     4081 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     4141 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     4201 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 
     4261 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     4321 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     4381 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     4441 aagggatttt ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt 
     4501 gttatgagcc atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg 
     4561 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca 
     4621 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt 
     4681 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg 
     4741 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact 
     4801 gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat 
     4861 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt 
     4921 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt 
     4981 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg 
     5041 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc 
     5101 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga 
     5161 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt 
     5221 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat 
     5281 aaattgcagt ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata 
     5341 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     5401 agtgccacct gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta 
     5461 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 
     5521 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 
     5581 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 
     5641 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 
     5701 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 
     5761 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 
     5821 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca 
//
