LOCUS       pGEX-6P-3-hSC35(61-115)-S98A        5152 bp    DNA     circular     28-JUN-2016
DEFINITION  LMBP 10243.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|664466805|
COMMENT     VNTDBDATE|-11301941|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGEX-6P-3-hSC35(61-115)-S98A|
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FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="pGEX-6P-3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        184..212
                     /vntifkey="30"
                     /label=tac_promoter
     misc_feature    224..246
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    258..944
                     /vntifkey="21"
                     /label=GST
     CDS             258..1164
                     /vntifkey="4"
                     /label=ORF\frame\3
     misc_feature    869..891
                     /vntifkey="21"
                     /label=pGEX_5_primer
     misc_feature    918..938
                     /vntifkey="21"
                     /label=precision
     misc_feature    complement(1202..1224)
                     /vntifkey="21"
                     /label=pGEX_3_primer
     promoter        1490..1518
                     /vntifkey="30"
                     /label=AmpR_promoter
     CDS             1560..2420
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 2"
     gene            1560..2420
                     /gene="Ampicillin"
                     /vntifkey="60"
     rep_origin      2575..3194
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    3492..4583
                     /vntifkey="21"
                     /label=lacI
     CDS             3624..4583
                     /vntifkey="4"
                     /label=ORF\frame\2
     promoter        4632..4661
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    4675..4697
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    4696..4714
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4726..4742)
                     /vntifkey="21"
                     /label=M13_forward20_primer
     misc_feature    complement(4735..4757)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    4723..4878
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    951..1121
                     /vntifkey="21"
                     /label=hSC35(AA61-115)
BASE COUNT     1258 a      1264 c      1359 g      1271 t 
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atgcgctttc 
      961 acgacaagcg cgacgctgag gacgctatgg atgccatgga cggggccgtg ctggacggcc 
     1021 gcgagctgcg ggtgcaaatg gcgcgctacg gccgcccccc ggacgcacac cacagccgcc 
     1081 ggggaccgcc accccgcagg tacgggggcg gtggctacta agaattcccg ggtcgactcg 
     1141 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa 
     1201 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 
     1261 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 
     1321 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac 
     1381 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 
     1441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     1501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     1561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     1621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     1681 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     1741 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     1801 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     1861 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     1921 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     1981 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     2041 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     2101 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     2161 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     2221 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     2281 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     2341 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     2401 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 
     2461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     2521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     2581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     2641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     2701 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 
     2761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     2821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     2881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     2941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     3001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     3061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     3121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     3181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
     3241 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 
     3301 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 
     3361 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt 
     3421 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 
     3481 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 
     3541 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 
     3601 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 
     3661 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 
     3721 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 
     3781 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 
     3841 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 
     3901 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 
     3961 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 
     4021 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 
     4081 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 
     4141 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 
     4201 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 
     4261 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 
     4321 acgataccga agacagctca tgttatatcc cgccgtcaac caccatcaaa caggattttc 
     4381 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 
     4441 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 
     4501 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     4561 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
     4621 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 
     4681 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt 
     4741 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     4801 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     4861 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga 
     4921 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca 
     4981 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc 
     5041 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga 
     5101 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt 
//

