LOCUS       pM2-Apex2               4935 bp    DNA     circular     17-AUG-2018
DEFINITION  LMBP 10253.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10253
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pM2-Apex2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                   -13.5909 10.454 0 -10 13.065 1.16519 #150#) 
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COMMENT         #179=(AnlzXLATItem 4874 4938 3 4874 1088) 
COMMENT         #180=(AnlzXLATItem -1 1088 3 4874 1088) 
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FEATURES             Location/Qualifiers
     promoter        4306..4873
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     terminator      1090..1167
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1090..1167)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(1221..1676)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        1677..1772
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     RBS             1773..1807
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        1828..2109
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      2066..2141
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             2201..2995
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      3186..3255
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      3973..4299
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             304..1089
                     /vntifkey="4"
                     /label=APX2
     CDS             1..291
                     /vntifkey="4"
                     /label=Influenza\M2
BASE COUNT     1173 a      1260 c      1299 g      1203 t 
ORIGIN
        1 atgagtcttc taaccgaggt cgaaacgcct atcagaaacg aatgggggtg cagatgcaac 
       61 gattcaagtg accctcttgt tgttgccgcg agtatcattg ggatcttgca cttgatattg 
      121 tggattcttg atcatctttt tttcaaatgc atttatcgct tctttaaaca cggtctgaaa 
      181 agagggcctt ctacggaagg agtaccagag tctatgaggg aagaatatcg aaaggaacag 
      241 cagagtgctg tggattctga cgatagtcat tttgtcagca tagagctgga gggatccggt 
      301 ggaggaaagt cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag 
      361 aagaagctca gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca 
      421 ttccactctg ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc 
      481 aagcaccctg ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt 
      541 ttggagccac tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct 
      601 ggcgttgttg ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag 
      661 gacaagcctg agccaccacc agagggtcgc ttgcccgatc ccactaaggg ttctgaccat 
      721 ttgagagatg tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct 
      781 gggggtcaca ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc 
      841 tctaatcctc ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa 
      901 ggtctccttc agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc 
      961 gttgacaaat atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa 
     1021 aagctttccg agcttgggtt tgctgatgcc ctgcagctgc ctcccctgga gcgcctgacc 
     1081 ctggactaag ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     1141 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     1201 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 
     1261 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     1321 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     1381 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     1441 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     1501 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     1561 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     1621 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg 
     1681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     1741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     1801 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 
     1861 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
     1921 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     1981 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     2041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     2101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     2161 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     2221 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     2281 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     2341 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 
     2401 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     2461 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     2521 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     2581 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     2641 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     2701 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 
     2761 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     2821 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     2881 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     2941 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     3001 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     3061 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     3121 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 
     3181 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 
     3241 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 
     3301 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 
     3361 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 
     3421 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 
     3481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     3541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     3601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     3661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     3721 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 
     3781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     3841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     3901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     3961 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     4021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     4081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     4141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     4201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     4261 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta 
     4321 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 
     4381 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 
     4441 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 
     4501 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 
     4561 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 
     4621 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 
     4681 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 
     4741 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 
     4801 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 
     4861 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc 
     4921 gagctcaagc ttacc 
//
