LOCUS       pJH2                    6356 bp    DNA     circular SYN  8-MAY-2017
DEFINITION  Broad host range translational fusion vector pJH2.
ACCESSION   FJ607244
VERSION     FJ607244.1
SOURCE      Broad host range translational fusion vector pJH2.
  ORGANISM  Broad host range translational fusion vector pJH2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Hamlin,J.N., Bloodworth,R.A. and Cardona,S.T.
  TITLE     Regulation of phenylacetic acid degradation genes of Burkholderia
            cenocepacia K56-2
  JOURNAL   BMC Microbiol. 9, 222 (2009)
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Hamlin,J.N.R. and Cardona,S.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2009) MIcrobiology, University of Manitoba, 418
            Buller Building, Winnipeg, Manitoba R3T 2N2, Canada
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11754072|
COMMENT     VNTDBDATE|-11754072|
COMMENT     LSOWNER|
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COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                         "Sequence Source: Broad host range translational fusion vector pJH2" 
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COMMENT                         "Organizm(s): <a href=\"ncbilink:taxonomy/Broad+host+range+translational+fusion+vector+pJH2\">Broad host range translational fusion vector pJH2(Broad host range translational fusion vector pJH2)</a>" 
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COMMENT                                    5.38787 6.09553 1 0.082322) 0.8 1.8 0) 
COMMENT                             #166=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   8.1452 10.454 0 -10 2.256 1.16519 #165#) 
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FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Broad host range translational fusion vector
                     pJH2"
                     /mol_type="other DNA"
                     /db_xref="taxon:651324"
                     /vntifkey="98"
                     /note="derived from Bordatella bronchiseptica plasmid pBBR1 genes for mobilization and replication"
     gene            <181..897
                     /gene="e-gfp"
                     /vntifkey="60"
     CDS             <181..897
                     /gene="e-gfp"
                     /codon_start=1
                     /transl_table=11
                     /product="green fluorescent protein"
                     /protein_id="ACS36609.1"
                     /vntifkey="4"
                     /label=EGFP
     gene            complement(5461..6099)
                     /gene="CAT"
                     /vntifkey="60"
     CDS             complement(5461..6099)
                     /gene="CAT"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="ACS36608.1"
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     terminator      complement(1133..1176)
                     /vntifkey="43"
                     /label=T1\terminator
     terminator      1308..1335
                     /vntifkey="43"
                     /label=T2\terminator
     CDS             complement(2303..3322)
                     /vntifkey="4"
                     /label=mob
     rep_origin      4316..4978
                     /vntifkey="33"
                     /label=pBBR1\ori
BASE COUNT     1380 a      1869 c      1751 g      1356 t 
ORIGIN
        1 atcttaggat ctcccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc 
       61 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta 
      121 ctcgagccat ggcatatgag tacgcccggg agatctggta cccgtactgg atccgaattc 
      181 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 
      241 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 
      301 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 
      361 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 
      421 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 
      481 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 
      541 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 
      601 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 
      661 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 
      721 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 
      781 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 
      841 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatct 
      901 agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc 
      961 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc 
     1021 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 
     1081 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 
     1141 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 
     1201 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg 
     1261 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct 
     1321 gacggatggc ctttttgcgt ttctacaaac tcttccactc actacagcag agccatttaa 
     1381 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt 
     1441 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc 
     1501 tggcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc 
     1561 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac 
     1621 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc 
     1681 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc 
     1741 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact 
     1801 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt 
     1861 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa 
     1921 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc 
     1981 cctcgtcaaa aataaggtta tcaagcgaga aatcaccatg agtgacgact gaatccggtg 
     2041 agaatggcaa aagctaatgc atagctcctg aaaatctcga taactcaaaa aatacgcccg 
     2101 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc 
     2161 attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt 
     2221 attctgcgaa gtgatcttcc gtcacaggta tttattcgaa gacgaaaggg cctcgtgata 
     2281 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 
     2341 tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg ggcctgtttc tggcgctgga 
     2401 cttcccgctg ttccgtcagc agcttttcgc ccacggcctt gatgatcgcg gcggccttgg 
     2461 cctgcatatc ccgattcaac ggccccaggg cgtccagaac gggcttcagg cgctcccgaa 
     2521 ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg catctcacgc gctcctgcgg 
     2581 cggcctgtag ggcaggctca tacccctgcc gaaccgcttt tgtcagccgg tcggccacgg 
     2641 cttccggcgt ctcaacgcgc tttgagattc ccagcttttc ggccaatccc tgcggtgcat 
     2701 aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg gcccactggt ggccgctcca 
     2761 gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt gctgccctcg atgccccgtt 
     2821 gcagccctag atcggccaca gcggccgcaa acgtggtctg gtcgcgggtc atctgcgctt 
     2881 tgttgccgat gaactccttg gccgacagcc tgccgtcctg cgtcagcggc accacgaacg 
     2941 cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc cgtcacgatg cgatccgccc 
     3001 cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa cgccgcctgc tgttcttggc 
     3061 tggccgactt ccaccattcc gggctggccg tcatgacgta ctcgaccgcc aacacagcgt 
     3121 ccttgcgccg cttctctggc agcaactcgc gcagtcggcc catcgcttca tcggtgctgc 
     3181 tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc agcgttgggc gtctcgcgct 
     3241 cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat tttcgccagc ttcttgcatc 
     3301 gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg gtgtccaacc ggctcgacgg 
     3361 gggcagcgca aggcggtgcc tccggcgggc cactcaatgc ttgagtatac tcactagact 
     3421 ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc ttgctctccg 
     3481 ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt ggacgatggc 
     3541 cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc 
     3601 tgatggacag gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca 
     3661 gccttcgacc acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt 
     3721 tttttaattt tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt 
     3781 tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt 
     3841 gacgcgcctg gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc 
     3901 gcctgccccc cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt 
     3961 gggcaaggcc gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg 
     4021 agggggagcc gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca 
     4081 aagaactaga tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg 
     4141 gtacgcaaca gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat 
     4201 ctgtaattga ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc 
     4261 agctgattgc gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc 
     4321 cacgcagtcc agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac 
     4381 ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat 
     4441 gctgctgcat cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa 
     4501 gacactttcc aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt 
     4561 ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta 
     4621 cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt 
     4681 cagtgccgcc gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga 
     4741 cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga 
     4801 ggagccgccc agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga 
     4861 aacggaggaa tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt 
     4921 tctggacgat ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagca 
     4981 cttgggttgc gcagcaaccc gtaagtgcgc tgttccagac tatcggctgt agccgcctcg 
     5041 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 
     5101 cgacctgaat ggaagccggc ggcacctcgc taacggattc accgttttta tcaggctctg 
     5161 ggaggcagaa taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa 
     5221 ctggcctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt 
     5281 aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc 
     5341 gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcac 
     5401 caggcgttta agtcgaccaa taactctacg gggtctgacg ctcagtggaa cgaaatcgat 
     5461 tcatcgcagt actgttgtat tcattaagca tctgccgaca tggaagccat cacaaacggc 
     5521 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc 
     5581 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt 
     5641 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa 
     5701 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg 
     5761 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac 
     5821 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg 
     5881 taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 
     5941 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 
     6001 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 
     6061 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 
     6121 aaatctcgac aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 
     6181 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 
     6241 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtag 
     6301 gcgcgccgaa gttcctatac ttatcgatct gcacttgaac gtgtggccta agcgat 
//
