LOCUS       pEXPR-myr-FLAG-hTRIM4        6434 bp    DNA     circular      1-JUN-2016
DEFINITION  LMBP 10359.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10359
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214227|
COMMENT     VNTDBDATE|-11263161|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM4|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM4'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:02" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5438..6298)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3137..3931
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4107..4181
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4107..4181)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4495..4897)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4320..4403)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      2970..3045
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2299..2727
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2029..2253
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2732..3013
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..1965
                     /vntifkey="4"
                     /label=hTRIM4
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      1966..1989
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1535 a      1667 c      1670 g      1562 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggaagct gaggacatcc aggaggagtt gacctgcccc atctgcctgg actatttcca 
     1141 ggacccggtg tccatcgagt gcggccacaa cttctgccgc ggctgcctgc accgcaactg 
     1201 ggcgccgggc ggcggcccgt tcccctgccc cgaatgtcgg cacccatcgg cgcccgccgc 
     1261 gctgcgaccc aactgggccc tggccaggct gactgagaag acgcagcgcc ggcgcctggg 
     1321 ccccgtgccc ccgggcctgt gcggccgcca ctgggagccg ctgcggctct tctgcgagga 
     1381 cgaccagcgg ccagtgtgcc tggtgtgcag ggagtcccag gagcaccaga ctcacgccat 
     1441 ggcacccatc gacgaggcct tcgagagcta ccgggagaaa cttcttaagt ctcagcgtaa 
     1501 tctcgtggcc aagatgaaga aagtcatgca tttacaggat gtagaagtga agaacgccac 
     1561 acagtggaag gataagataa agagtcagcg aatgagaatc agcacggagt tttcaaagct 
     1621 gcacaacttc ctggttgaag aagaggacct gtttcttcag agattgaaca aagaagaaga 
     1681 agagacgaag aagaagctga atgagaacac gttaaaactc aatcaaacta tcgcttcatt 
     1741 gaagaagctc atcttagagg tgggggagaa gagccaggct cccaccctgg agctgcttca 
     1801 gaatccaaaa gaagtgttga ccaggagtga gatccaggat gtgaactatt ctcttgaagc 
     1861 tgtaaaggtg aagacagtgt gccagatacc attgatgaag gaaatgctaa agcgattcca 
     1921 agcaactaaa ataaaaacac aaaaacaaaa caggcacaat atgtgcccaa ctttcttgta 
     1981 caaagtggtg atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta 
     2041 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 
     2101 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 
     2161 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 
     2221 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 
     2281 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 
     2341 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 
     2401 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 
     2461 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 
     2521 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 
     2581 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 
     2641 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 
     2701 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 
     2761 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     2821 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     2881 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 
     2941 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     3001 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     3061 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 
     3121 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 
     3181 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 
     3241 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 
     3301 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 
     3361 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 
     3421 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 
     3481 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 
     3541 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 
     3601 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 
     3661 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 
     3721 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 
     3781 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 
     3841 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 
     3901 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg 
     3961 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 
     4021 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 
     4081 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 
     4141 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     4201 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 
     4261 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     4321 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     4381 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     4441 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     4501 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     4561 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     4621 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     4681 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     4741 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     4801 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     4861 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     4921 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     4981 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     5041 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     5101 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     5161 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     5221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     5281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     5341 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     5401 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     5461 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     5521 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     5581 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     5641 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     5701 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     5761 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     5821 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     5881 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     5941 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     6001 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     6061 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     6121 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     6181 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     6241 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     6301 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     6361 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     6421 tgccacctga cgtc 
//

