LOCUS       pEXPR-myr-FLAG-hTRIM5        6593 bp    DNA     circular      1-JUN-2016
DEFINITION  LMBP 10360.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10360
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214229|
COMMENT     VNTDBDATE|-11263190|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM5|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM5'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:04" 1) 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     CDS             complement(5597..6457)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3296..4090
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4266..4340
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4266..4340)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4654..5056)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4479..4562)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3129..3204
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2458..2886
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2188..2412
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2891..3172
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2124
                     /vntifkey="4"
                     /label=hTRIM5
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      2125..2148
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1588 a      1665 c      1701 g      1639 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggcttct ggaatcctgg ttaatgtaaa ggaggaggtg acctgcccca tctgcctgga 
     1141 actcctgaca caacccctga gcctggactg cggccacagc ttctgccaag catgcctcac 
     1201 tgcaaaccac aagaagtcca tgctagacaa aggagagagt agctgccctg tgtgccggat 
     1261 cagttaccag cctgagaaca tacggcctaa tcggcatgta gccaacatag tggagaagct 
     1321 cagggaggtc aagttgagcc cagaggggca gaaagttgat cattgtgcac gccatggaga 
     1381 gaaacttcta ctcttctgtc aggaggacgg gaaggtcatt tgctggcttt gtgagcggtc 
     1441 tcaggagcac cgtggtcacc acacgttcct cacagaggag gttgcccggg agtaccaagt 
     1501 gaagctccag gcagctctgg agatgctgag gcagaagcag caggaagctg aagagttgga 
     1561 agctgacatc agagaagaga aagcttcctg gaagactcaa atacagtatg acaaaaccaa 
     1621 cgtcttggca gattttgagc aactgagaga catcctggac tgggaggaga gcaatgagct 
     1681 gcaaaacctg gagaaggagg aggaagacat tctgaaaagc cttacgaact ctgaaactga 
     1741 gatggtgcag cagacccagt ccctgagaga gctcatctca gatctggagc atcggctgca 
     1801 ggggtcagtg atggagctgc ttcagggtgt ggatggcgtc ataaaaagga cggagaacgt 
     1861 gaccttgaag aagccagaaa cttttccaaa aaatcaaagg agagtgtttc gagctcctga 
     1921 tctgaaagga atgctagaag tgtttagaga gctgacagat gtccgacgct actggggtaa 
     1981 ggagaagtca cattatcata agccaccctg cggcttatca ttattattat ctttatcttt 
     2041 tagaatttta tgttctctat taggctcatg ttttaagatt tatgattctc cttccaagac 
     2101 acacataact tacccctcct tatacccaac tttcttgtac aaagtggtga tctagagggc 
     2161 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 
     2221 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
     2281 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 
     2341 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 
     2401 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 
     2461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     2521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     2581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     2641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     2701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     2761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     2821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     2881 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 
     2941 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
     3001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
     3061 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     3121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 
     3181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 
     3241 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 
     3301 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 
     3361 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 
     3421 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 
     3481 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 
     3541 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 
     3601 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 
     3661 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 
     3721 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 
     3781 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 
     3841 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 
     3901 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 
     3961 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 
     4021 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 
     4081 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc 
     4141 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 
     4201 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 
     4261 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     4321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     4381 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 
     4441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     4501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 
     4561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 
     4621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 
     4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     5881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     5941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     6001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     6061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     6121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     6181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     6241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     6301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     6361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     6421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     6481 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     6541 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc 
//

