LOCUS       pEXPR-myr-FLAG-hTRIM9        7202 bp    DNA     circular      1-JUN-2016
DEFINITION  LMBP 10361.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10361
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214231|
COMMENT     VNTDBDATE|-11263211|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM9|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM9'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 10361." 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:06" 1) 
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COMMENT                          "Last Modified: 06/01/16 03:26" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6206..7066)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3905..4699
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4875..4949
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4875..4949)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5263..5665)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(5088..5171)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3738..3813
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      3067..3495
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2797..3021
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3500..3781
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2733
                     /vntifkey="4"
                     /label=hTRIM9
     attenuator      2734..2757
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1664 a      1945 c      1881 g      1712 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggaggag atggaagagg agttgaaatg ccccgtgtgc ggctccttct atcgggagcc 
     1141 catcatcctg ccctgctctc acaatttgtg tcaggcgtgc gcccgcaaca tcctggtgca 
     1201 gaccccagag tctgaatccc cccagagcca tcgggccgcg ggctccgggg tctccgacta 
     1261 tgactatctg gacctggaca agatgagcct atacagcgag gcggacagcg gctatggctc 
     1321 ctacgggggg ttcgccagcg cccccactac cccgtgccag aagtccccca acggcgtccg 
     1381 cgtgtttccc ccggctatgc cgccaccggc cacccacttg tcaccggccc tggccccggt 
     1441 gccccgcaac tcctgtatca cctgccccca gtgtcaccgc agcctcatcc tggatgaccg 
     1501 ggggctccgc ggcttcccca agaatcgcgt actggaaggg gtaattgacc gctaccagca 
     1561 gagcaaagcc gcggccctca agtgccagct ctgcgagaag gcgcccaagg aagccaccgt 
     1621 catgtgcgaa cagtgcgatg tcttctactg cgatccgtgc cgcctgcgct gccacccgcc 
     1681 ccgggggccc ctagccaagc accgcctggt gcccccggcc cagggtcgtg tgagccggag 
     1741 gctgagccca cgcaaggtct ccacctgcac agaccacgag ctggagaacc acagcatgta 
     1801 ctgcgtgcaa tgcaagatgc ccgtgtgcta ccagtgcttg gaggagggca aacactccag 
     1861 ccacgaagtc aaggctctgg gggccatgtg gaaactacat aagagccagc tctcccaggc 
     1921 gctgaacgga ctgtcagaca gggccaaaga agccaaggag tttctggtac agctgcgcaa 
     1981 catggtccag cagatccagg agaacagtgt ggagtttgaa gcctgtctgg tggcccaatg 
     2041 tgatgccctc atcgatgccc tcaacagaag aaaagcccag ctgctggccc gcgtcaacaa 
     2101 ggagcatgag cacaagctga aggtggttcg agatcagatc tctcactgca cagtgaaatt 
     2161 gcgccagacc acaggtctca tggagtactg cttggaggtg attaaggaaa atgatcctag 
     2221 tggttttttg cagatttctg acgccctcat aagaagagtg cacctgactg aggatcagtg 
     2281 gggtaaaggc acactcactc caaggatgac cacggacttt gacttgagtc tggacaacag 
     2341 ccctctgctg caatccatcc accagctgga tttcgtgcaa gtgaaagctt cctctccagt 
     2401 cccagcaacc cctatcctac agctggagga atgttgtacc cacaacaaca gcgctacgtt 
     2461 gtcctggaaa cagccacctc tgtccacggt gcccgccgat ggatacattc tggagctgga 
     2521 tgatggcaac ggtggtcaat tccgggaggt gtatgtgggg aaggagacaa tgtgcactgt 
     2581 ggatggtctt cacttcaaca gcacatacaa cgctcgggtc aaggccttca acaaaacagg 
     2641 agtcagcccg tacagcaaga ccctggtcct ccaaacgtct gagggtaagg cccttcagca 
     2701 gtatccctca gagcgagaac tgcgtggcat ctacccaact ttcttgtaca aagtggtgat 
     2761 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat 
     2821 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 
     2881 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 
     2941 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 
     3001 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt 
     3061 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
     3121 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
     3181 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
     3241 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
     3301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
     3361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
     3421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
     3481 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
     3541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     3601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     3721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 
     3781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     3841 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 
     3901 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 
     3961 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 
     4021 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 
     4081 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 
     4141 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 
     4201 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 
     4261 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 
     4321 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 
     4381 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 
     4441 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 
     4501 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 
     4561 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 
     4621 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 
     4681 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc 
     4741 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
     4801 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 
     4861 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     4921 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     4981 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 
     5041 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
     5101 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     5161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     5221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     5281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     5341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     5401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     5461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     5521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     5581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     5641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     5701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     5761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     5821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     5881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     5941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     6001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     6061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     6121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     6181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     6241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     6301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     6361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     6421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     6481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     6541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     6601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     6661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     6721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     6781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     6841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     6901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     6961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     7021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     7081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     7141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     7201 tc 
//

