LOCUS       pEXPR-myr-FLAG-hTRIM10        6740 bp    DNA     circular      7-JUN-2016
DEFINITION  LMBP 10362.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10362
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214073|
COMMENT     VNTDBDATE|-11271631|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM10|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 12:28" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5744..6604)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3443..4237
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4413..4487
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4413..4487)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4801..5203)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4626..4709)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3276..3351
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2605..3033
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2335..2559
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3038..3319
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      2272..2295
                     /vntifkey="0"
                     /label=attB2
     CDS             1085..2271
                     /vntifkey="4"
                     /label=TRIM10
BASE COUNT     1580 a      1747 c      1761 g      1652 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 caccatggcc tctgctgcct ctgtgaccag cctggcagat gaagtcaact gccccatctg 
     1141 tcagggtacc ctgagggagc cggtcactat cgactgcggc cacaacttct gccgggcctg 
     1201 ccttacccgc tactgtgaga taccaggccc agacctggag gagtccccta cttgcccact 
     1261 ctgcaaagaa cccttccgtc ctgggagctt ccggcccaac tggcagctgg ctaacgtggt 
     1321 ggagaacatt gagcgcctcc agctggtgtc cacactgggt ttgggagagg aggatgtctg 
     1381 ccaagagcac ggagagaaga tctacttctt ctgtgaggat gatgagatgc agttgtgcgt 
     1441 ggtgtgccgg gaggctgggg agcacgctac ccacaccatg cgcttcctgg aggatgcagc 
     1501 ggctccctat agggaacaaa tccataagtg tcttaaatgt ctaagaaaag agagagagga 
     1561 gattcaagaa atccagtcaa gagaaaataa aaggatgcaa gtcctcctga ctcaggtgtc 
     1621 caccaagaga caacaggtga tttctgagtt cgcacacctg aggaagtttc tagaggaaca 
     1681 gcagagcatc ctcttagcac aattggagag ccaggatggg gacatcttga ggcaacggga 
     1741 tgaatttgat ttgctggttg ctggggagat ctgccggttt agtgctctta ttgaagaact 
     1801 ggaggagaag aatgagaggc cagcaaggga gctcctgacg gacatcagaa gcactctaat 
     1861 aagatgtgaa accagaaagt gccggaaacc ggtggctgtg tcgccagagc tgggccagag 
     1921 gattcgggac tttccccagc aggccctccc gctgcagagg gagatgaaga tgtttctgga 
     1981 aaaactatgc tttgagttgg actatgagcc agctcacatt tctctagacc ctcagacttc 
     2041 ccaccccaag ctcctcttgt ccgaggacca ccagcgagct cagttctcct acaaatggca 
     2101 gaactcacca gacaaccccc agcgttttga ccgggccacc tgtgttctgg cccacactgg 
     2161 catcacaggg gggagacaca cgtgggtgtg gatggccagg gtacctgggg actcaggctg 
     2221 ctgccagttc tgctcaccac catccgtgct tggcacagaa gtagctgcat tgccaacttc 
     2281 cttgtacaaa gtggtgatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc 
     2341 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
     2401 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
     2461 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
     2521 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
     2581 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     2641 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     2701 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     2761 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     2821 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     2881 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     2941 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 
     3001 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
     3061 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     3121 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     3181 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     3241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     3301 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     3361 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     3421 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     3481 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     3541 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     3601 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     3661 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     3721 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     3781 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     3841 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     3901 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     3961 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     4021 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     4081 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     4141 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     4201 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa 
     4261 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 
     4321 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     4381 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 
     4441 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4501 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 
     4561 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 
     4621 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 
     4681 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 
     4741 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     4801 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     4861 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     4921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     4981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     5041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     5101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     5161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     5221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     5281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     5341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     5401 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     5461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     5521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     5581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     5641 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     5701 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     5761 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     5821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     5881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     5941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     6001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     6061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     6121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     6181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     6241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     6301 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     6361 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     6421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     6481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     6541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     6601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     6661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     6721 gaaaagtgcc acctgacgtc 
//

