LOCUS       pEXPR-myr-FLAG-hTRIM11        6158 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10363.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10363
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214234|
COMMENT     VNTDBDATE|-11264326|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM11|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM11'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:09" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5162..6022)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             2861..3655
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      3831..3905
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3831..3905)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4219..4621)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4044..4127)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      2694..2769
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2023..2451
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      1753..1977
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2456..2737
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..1689
                     /vntifkey="4"
                     /label=hTRIM11
     attenuator      1690..1713
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1391 a      1626 c      1623 g      1518 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggagctg aggaccgtgt gcagggtccc gggactggta gagacactgc ggaggtttcg 
     1141 aggggacgtg accttggacc cggacaccgc caaccctgag ctgatcctgt ctgaagacag 
     1201 gcggagcgtg cagcgggggg acctacggca ggccctgccg gacagcccag agcgctttga 
     1261 ccccggcccc tgcgtgctgg gccaggagcg cttcacctca ggccgccact actgggaggt 
     1321 ggaggttggg gaccgcacca gctgggccct gggggtgtgc agggagaacg tgaacaggaa 
     1381 ggagaagggc gagctgtccg cgggcaacgg cttctggatc ctggtcttcc tggggagcta 
     1441 ttacaattcc tcggaacggg ccttggctcc actccgggac ccacccaggc gcgtggggat 
     1501 ctttctggac tacgaggctg gacatctctc tttctacagt gccaccgatg ggtcactgct 
     1561 attcatcttt cccgagatcc ccttctcggg gacgctgcgg cccctcttct cacccctgtc 
     1621 cagcagcccg accccgatga ctatctgccg gccgaaaggt gggtccgggg acaccctggc 
     1681 tccccagtgc ccaactttct tgtacaaagt ggtgatctag agggcccgtt taaacccgct 
     1741 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
     1801 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
     1861 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
     1921 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 
     1981 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 
     2041 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     2101 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     2161 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
     2221 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     2281 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     2341 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
     2401 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 
     2461 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     2521 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 
     2581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     2641 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     2701 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 
     2761 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 
     2821 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     2881 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     2941 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     3001 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 
     3061 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     3121 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     3181 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     3241 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     3301 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     3361 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 
     3421 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     3481 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     3541 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     3601 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     3661 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 
     3721 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 
     3781 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 
     3841 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     3901 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     3961 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     4021 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     4081 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 
     4141 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 
     4201 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     4261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     4321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     4381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     4441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     4501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     4561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     4621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     4681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     4741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     4801 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 
     4861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     4921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     4981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     5041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     5101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     5161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     5221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     5281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     5341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     5401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     5461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     5521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     5581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     5641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     5701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     5761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     5821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     5881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     5941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     6001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     6061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     6121 ttccgcgcac atttccccga aaagtgccac ctgacgtc 
//

