LOCUS       pEXPR-myr-FLAG-hTRIM15        6947 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10365.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10365
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214236|
COMMENT     VNTDBDATE|-11264343|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM15|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM15'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:11" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5951..6811)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3650..4444
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4620..4694
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4620..4694)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5008..5410)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4833..4916)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3483..3558
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2812..3240
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2542..2766
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3245..3526
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2478
                     /vntifkey="4"
                     /label=hTRIM15
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      2479..2502
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1585 a      1836 c      1861 g      1665 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catgcccgca accccgtccc tgaaggtggt ccatgagctg cctgcctgta ccctctgtgc 
     1141 ggggccgctg gaggatgcgg tgaccattcc ctgtggacac accttctgcc ggctctgcct 
     1201 ccccgcgctc tcccagatgg gggcccaatc ctcgggcaag atcctgctct gcccgctctg 
     1261 ccaagaggag gagcaggcag agactcccat ggcccctgtg cccctgggcc cgctgggaga 
     1321 aacttactgc gaggagcacg gcgagaagat ctacttcttc tgcgagaacg atgccgagtt 
     1381 cctctgtgtg ttctgcaggg agggtcccac gcaccaggcg cacaccgtgg ggttcctgga 
     1441 cgaggccatt cagccctacc gggatcgtct caggagtcga ctggaagctc tgagcacgga 
     1501 gagagatgag attgaggatg taaagtgtca agaagaccag aagcttcaag tgctgctgac 
     1561 tcagatcgaa agcaagaagc atcaggtgga aacagctttt gagaggctgc agcaggagct 
     1621 ggagcagcag cgatgtctcc tgctggccag gctgagggag ctggagcagc agatttggaa 
     1681 ggagagggat gaatatatca caaaggtctc tgaggaagtc acccggcttg gagcccaggt 
     1741 caaggagctg gaggagaagt gtcagcagcc agcaagtgag cttctacaag atgtcagagt 
     1801 caaccagagc aggtgtgaga tgaagacttt tgtgagtcct gaggccattt ctcctgacct 
     1861 tgtcaagaag atccgtgatt tccacaggaa aatactcacc ctcccagaga tgatgaggat 
     1921 gttctcagaa aacttggcgc atcatctgga aatagattca ggggtcatca ctctggaccc 
     1981 tcagaccgcc agccggagcc tggttctctc ggaagacagg aagtcagtga ggtacacccg 
     2041 gcagaagaag aacctgccag acagccccct gcgcttcgac ggcctcccgg cggttctggg 
     2101 cttcccgggc ttctcctccg ggcgccaccg ctggcaggtt gacctgcagc tgggcgacgg 
     2161 cggcggctgc acggtggggg tggccgggga gggggtgagg aggaagggag agatgggact 
     2221 cagcgccgag gacggcgtct gggccgtgat catctcgcac cagcagtgct gggccagcac 
     2281 ctccccgggc accgacctgc cgctgagcga gatcccgcgc ggcgtgagag tcgccctgga 
     2341 ctacgaggcg gggcaggtga ccctccacaa cgcccagacc caggagccca tcttcacctt 
     2401 cactgcctct ttctccggca aagtcttccc tttctttgcc gtctggaaaa aaggttcctg 
     2461 ccttacgctg aaaggctgcc caactttctt gtacaaagtg gtgatctaga gggcccgttt 
     2521 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     2701 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     2761 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 
     2821 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     2881 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     2941 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     3001 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     3061 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     3121 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     3181 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     3241 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     3301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     3361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     3421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     3481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 
     3541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 
     3601 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 
     3661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 
     3721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 
     3781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 
     3841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 
     3901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 
     3961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 
     4021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 
     4081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 
     4141 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 
     4201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 
     4261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 
     4321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 
     4381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 
     4441 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac 
     4501 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 
     4561 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 
     4621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     4681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     4741 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     4801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     4861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     4921 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     4981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     5041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     5101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     5161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     5221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     5281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     5341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     5401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     5461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     5521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     5581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     5641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     5701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     5761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     5821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     5881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     6541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     6901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//

