LOCUS       pEXPR-myr-FLAG-hTRIM16        7244 bp    DNA     circular      7-JUN-2016
DEFINITION  LMBP 10366.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10366
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214240|
COMMENT     VNTDBDATE|-11271783|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM16|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM16'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:15" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6248..7108)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3947..4741
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4917..4991
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4917..4991)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5305..5707)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(5130..5213)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3780..3855
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      3109..3537
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2839..3063
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3542..3823
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     attenuator      2776..2799
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     CDS             1082..2775
                     /vntifkey="4"
                     /label=TRIM16
BASE COUNT     1713 a      1890 c      1898 g      1743 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggctgag ttggatctaa tggctccagg gccactgccc agggccactg ctcagccccc 
     1141 agcccctctc agcccagact ctgggtcacc cagcccagat tctgggtcag ccagcccagt 
     1201 ggaagaagag gacgtgggct cctcggagaa gcttggcagg gagacggagg aacaggacag 
     1261 cgactctgca gagcaggggg atcctgctgg tgaggggaaa gaggtcctgt gtgacttctg 
     1321 ccttgatgac accagaagag tgaaggcagt gaagtcctgt ctaacctgca tggtgaatta 
     1381 ctgtgaagag cacttgcagc cgcatcaggt gaacatcaaa ctgcaaagcc acctgctgac 
     1441 cgagccagtg aaggaccaca actggcgata ctgccctgcc caccacagcc cactgtctgc 
     1501 cttctgctgc cctgatcagc agtgcatctg ccaggactgt tgccaggagc acagtggcca 
     1561 caccatagtc tccctggatg cagcccgcag ggacaaggag gctgaactcc agtgcaccca 
     1621 gttagacttg gagcggaaac tcaagttgaa tgaaaatgcc atctccaggc tccaggctaa 
     1681 ccaaaagtct gttctggtgt cggtgtcaga ggtcaaagcg gtggctgaaa tgcagtttgg 
     1741 ggaactcctt gctgctgtga ggaaggccca ggccaatgtg atgctcttct tagaggagaa 
     1801 ggagcaagct gcgctgagcc aggccaacgg tatcaaggcc cacctggagt acaggagtgc 
     1861 cgagatggag aagagcaagc aggagctgga gaggatggcg gccatcagca acactgtcca 
     1921 gttcttggag gagtactgca agtttaagaa cactgaagac atcaccttcc ctagtgttta 
     1981 cgtagggctg aaggataaac tctcgggcat ccgcaaagtt atcacggaat ccactgtaca 
     2041 cttaatccag ttgctggaga actataagaa aaagctccag gagttttcca aggaagagga 
     2101 gtatgacatc agaactcaag tgtctgccgt tgttcagcgc aaatattgga cttccaaacc 
     2161 tgagcccagc accagggaac agttcctcca atatgcgtat gacatcacgt ttgacccgga 
     2221 cacagcacac aagtatctcc ggctgcagga ggagaaccgc aaggtcacca acaccacgcc 
     2281 ctgggagcat ccctacccgg acctccccag caggttcctg cactggcggc aggtgctgtc 
     2341 ccagcagagt ctgtacctgc acaggtacta ttttgaggtg gagatcttcg gggcaggcac 
     2401 ctatgttggc ctgacctgca aaggcatcga ccggaaaggg gaggagcgca acagttgcat 
     2461 ttccggaaac aacttctcct ggagcctcca atggaacggg aaggagttca cggcctggta 
     2521 cagtgacatg gagaccccac tcaaagctgg ccctttccgg aggctcgggg tctatatcga 
     2581 cttcccggga gggatccttt ccttctatgg cgtagagtat gataccatga ctctggttca 
     2641 caagtttgcc tgcaaatttt cagaaccagt ctatgctgcc ttctggcttt ccaagaagga 
     2701 aaacgccatc cggattgtag atctgggaga ggaacccgag aagccagcac cgtccttggt 
     2761 ggtgactgct ccctacccaa ctttcttgta caaagtggtg atctagaggg cccgtttaaa 
     2821 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     2881 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     2941 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     3001 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 
     3061 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 
     3121 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     3181 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     3241 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     3301 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     3361 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     3421 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 
     3481 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 
     3541 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     3601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     3721 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     3781 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
     3841 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 
     3901 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     3961 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     4021 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     4081 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
     4141 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     4201 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     4261 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     4321 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     4381 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     4441 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 
     4501 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     4561 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     4621 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     4681 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     4741 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 
     4801 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 
     4861 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 
     4921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     4981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     5041 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 
     5101 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     5161 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     5221 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     5281 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     5341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     5401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     5461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     5521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     5581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     5641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     5701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     5761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     5821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     5881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     5941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     6001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     6061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     6121 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     6181 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     6241 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     6301 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     6361 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     6421 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     6481 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     6541 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     6601 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     6661 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     6721 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     6781 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     6841 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     6901 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     6961 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     7021 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     7081 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     7141 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     7201 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc 
//

