LOCUS pEXPR-myr-FLAG-hTRIM23 7274 bp DNA circular 2-JUN-2016 DEFINITION LMBP 10367. SOURCE ORGANISM COMMENT Accession number: LMBP 10367 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11214245| COMMENT VNTDBDATE|-11264387| COMMENT LSOWNER| COMMENT VNTNAME|pEXPR-myr-FLAG-hTRIM23| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT (CObjectList) (CObjectList)) COMMENT #214=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -890 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) -2147483648 COMMENT (CStringList) 2147483648 268632118 (CObList))) FEATURES Location/Qualifiers CDS complement(6278..7138) /vntifkey="4" /label=ampicillin\resistance CDS 3977..4771 /vntifkey="4" /label=Tn5\neomycin\resistance terminator 4947..5021 /vntifkey="43" /label=SV40\polyA\early terminator complement(4947..5021) /vntifkey="43" /label=SV40\polyA\late rep_origin complement(5335..5737) /vntifkey="33" /label=pMB1\ori promoter complement(5160..5243) /vntifkey="30" /label=lac\promoter promoter 235..818 /vntifkey="29" /label=hCMV-IE\promoter\and\enhancer rep_origin 3810..3885 /vntifkey="33" /label=SV40\ori rep_origin 3139..3567 /vntifkey="33" /label=f1\ori promoter 863..879 /vntifkey="30" /label=T7\promoter misc_marker 968..991 /vntifkey="22" /label=FLAG\epitope terminator 2869..3093 /vntifkey="43" /label=BGH\polyA promoter 3572..3853 /vntifkey="29" /label=SV40\early\promoter CDS 914..952 /vntifkey="4" /label=mLck\myristoylation-targeting\sequence CDS 1082..2805 /vntifkey="4" /label=hTRIM23 attenuator 1058..1081 /vntifkey="0" /label=attB1 attenuator 2806..2829 /vntifkey="0" /label=attB2 BASE COUNT 1789 a 1753 c 1847 g 1885 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 1081 catggctacc ctggttgtaa acaagctcgg agcgggagta gacagtggcc ggcagggcag 1141 ccgggggaca gctgtagtga aggtgctaga gtgtggagtt tgtgaagatg tcttttcttt 1201 gcaaggagac aaagttcccc gtcttttgct ttgtggccat accgtctgtc atgactgtct 1261 cactcgccta cctcttcatg gaagagcaat ccgttgccca tttgatcgac aagtaacaga 1321 cctaggtgat tcaggtgtct ggggattgaa aaaaaatttt gctttattgg agcttttgga 1381 acgactgcag aatgggccta ttggtcagta tggagctgca gaagaatcca ttgggatatc 1441 tggagagagc atcattcgtt gtgatgaaga tgaagctcac cttgcctctg tatattgcac 1501 tgtgtgtgca actcatttgt gctctgagtg ttctcaagtt actcattcta caaagacatt 1561 agcaaagcac aggcgagttc ctctagctga taaacctcat gagaaaacta tgtgctctca 1621 gcaccaggtg catgccattg agtttgtttg cttggaagaa ggttgtcaaa ctagcccact 1681 catgtgctgt gtctgcaaag aatatggaaa acaccagggt cacaagcatt cagtattgga 1741 accagaagct aatcagatcc gagcatcaat tttagatatg gctcactgca tacggacctt 1801 cacagaggaa atctcagatt attccagaaa attagttgga attgtgcagc acattgaagg 1861 aggagaacaa atcgtggaag atggaattgg aatggctcac acagaacatg taccagggac 1921 tgcagagaat gcccggtcat gtattcgagc ttatttttat gatctacatg aaactctgtg 1981 tcgtcaagaa gaaatggctc taagtgttgt tgatgctcat gttcgtgaaa aattgatttg 2041 gctcaggcag caacaagaag atatgactat tttgttgtca gaggtttctg cagcctgcct 2101 ccactgtgaa aagactttgc agcaggatga ttgtagagtt gtcttggcaa aacaggaaat 2161 tacaaggtta ctggaaacat tgcagaaaca gcagcagcag tttacagaag ttgcagatca 2221 cattcagttg gatgccagca tccctgtcac ttttacaaag gataatcgag ttcacattgg 2281 accaaaaatg gaaattcggg tcgttacgtt aggattggat ggtgctggaa aaactactat 2341 cttgtttaag ttaaaacagg atgaattcat gcagcccatt ccaacaattg gttttaacgt 2401 ggaaactgta gaatataaaa atctaaaatt cactatttgg gatgtaggtg gaaaacacaa 2461 attaagacca ttgtggaaac attattacct caatactcaa gctgttgtgt ttgttgtaga 2521 tagcagtcat agagacagaa ttagtgaagc acacagcgaa cttgcaaagt tgttaacgga 2581 aaaagaactc cgagatgctc tgctcctgat ttttgctaac aaacaggatg ttgctggagc 2641 actgtcagta gaagaaatca ctgaactact cagtctccat aaattatgct gtggccgtag 2701 ctggtatatt cagggctgtg atgctcgaag tggtatggga ctgtatgaag ggttggactg 2761 gctctcacgg caacttgtag ctgctggagt attggatgtt gcttgcccaa ctttcttgta 2821 caaagtggtg atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta 2881 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 2941 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 3001 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 3061 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 3121 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3181 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3241 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 3301 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 3361 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 3421 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 3481 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 3541 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 3601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 3661 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3721 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 3781 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 3841 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 3901 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 3961 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 4021 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 4081 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 4141 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 4201 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 4261 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 4321 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 4381 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 4441 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 4501 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 4561 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 4621 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 4681 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 4741 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg 4801 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 4861 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 4921 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 4981 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 5041 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 5101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 5221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 5281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 5341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 5581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 5641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 5701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 5761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 5821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 5881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 5941 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 6001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 6061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 6121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 6181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 6241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 6301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 6361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 6421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 6481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 6541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 6601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 6661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 6721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 6781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 6841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 6901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 6961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 7021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 7081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 7141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 7201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 7261 tgccacctga cgtc //