LOCUS       pEXPR-myr-FLAG-hTRIM29        7316 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10368.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10368
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214251|
COMMENT     VNTDBDATE|-11264393|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM29|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM29'" 1) 
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COMMENT                          "Created: 04/28/16 03:26" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6320..7180)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             4019..4813
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4989..5063
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4989..5063)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5377..5779)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(5202..5285)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3852..3927
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      3181..3609
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2911..3135
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3614..3895
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2847
                     /vntifkey="4"
                     /label=hTRIM29
     attenuator      2848..2871
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1717 a      1945 c      1952 g      1702 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggaagct gcagatgcct ccaggagcaa cgggtcgagc ccagaagcca gggatgcccg 
     1141 gagcccgtcg ggccccagtg gcagcctgga gaatggcacc aaggctgacg gcaaggatgc 
     1201 caagaccacc aacgggcacg gcggggaggc agctgagggc aagagcctgg gcagcgccct 
     1261 gaagccaggg gaaggtagga gcgccctgtt cgcgggcaat gagtggcggc gacccatcat 
     1321 ccagtttgtc gagtccgggg acgacaagaa ctccaactac ttcagcatgg actctatgga 
     1381 aggcaagagg tcgccgtacg cagggctcca gctgggggct gccaagaagc cacccgttac 
     1441 ctttgccgaa aagggcgagc tgcgcaagtc cattttctcg gagtcccgga agcccacggt 
     1501 gtccatcatg gagcccgggg agacccggcg gaacagctac ccccgggccg acacgggcct 
     1561 tttttcacgg tccaagtccg gctccgagga ggtgctgtgc gactcctgca tcggcaacaa 
     1621 gcagaaggcg gtcaagtcct gcctggtgtg ccaggcctcc ttctgcgagc tgcatctcaa 
     1681 gccccacctg gagggcgccg ccttccgaga ccaccagctg ctcgagccca tccgggactt 
     1741 tgaggcccgc aagtgtcccg tgcatggcaa gacgatggag ctcttctgcc agaccgacca 
     1801 gacctgcatc tgctaccttt gcatgttcca ggagcacaag aatcatagca ccgtgacagt 
     1861 ggaggaggcc aaggccgaga aggagacgga gctgtcactg caaaaggagc agctgcagct 
     1921 caagatcatt gagattgagg atgaagctga gaagtggcag aaggagaagg accgcatcaa 
     1981 gagcttcacc accaatgaga aggccatcct ggagcagaac ttccgggacc tggtgcggga 
     2041 cctggagaag caaaaggagg aagtgagggc tgcgctggag cagcgggagc aggatgctgt 
     2101 ggaccaagtg aaggtgatca tggatgctct ggatgagaga gccaaggtgc tgcatgagga 
     2161 caagcagacc cgggagcagc tgcatagcat cagcgactct gtgttgtttc tgcaggaatt 
     2221 tggtgcattg atgagcaatt actctctccc cccacccctg cccacctatc atgtcctgct 
     2281 ggagggggag ggcctgggac agtcactagg caacttcaag gacgacctgc tcaatgtatg 
     2341 catgcgccac gttgagaaga tgtgcaaggc ggacctgagc cgtaacttca ttgagaggaa 
     2401 ccacatggag aacggtggtg accatcgcta tgtgaacaac tacacgaaca gcttcggggg 
     2461 tgagtggagt gcaccggaca ccatgaagag atactccatg tacctgacac ccaaaggtgg 
     2521 ggtccggaca tcataccagc cctcgtctcc tggccgcttc accaaggaga ccacccagaa 
     2581 gaatttcaac aatctctatg gcaccaaagg taactacacc tcccgggtct gggagtactc 
     2641 ctccagcatt cagaactctg acaatgacct gcccgtcgtc caaggcagct cctccttctc 
     2701 cctgaaaggc tatccctccc tcatgcggag ccaaagcccc aaggcccagc cccagacttg 
     2761 gaaatctggc aagcagacta tgctgtctca ctaccggcca ttctacgtca acaaaggcaa 
     2821 cgggattggg tccaacgaag ccccatgccc aactttcttg tacaaagtgg tgatctagag 
     2881 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     2941 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     3001 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     3061 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     3121 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     3181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     3241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     3301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     3361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     3421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     3481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     3541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     3601 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     3721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     3781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     3841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     3901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     3961 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     4021 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     4081 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     4141 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     4201 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     4261 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     4321 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     4381 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     4441 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     4501 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     4561 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     4621 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     4681 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     4741 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     4801 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 
     4861 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     4921 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     4981 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     5041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     5101 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     5161 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     5221 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     5281 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     5341 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     5401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     5461 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     5521 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     5581 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     5641 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     5701 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     5761 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     5821 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     5881 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     5941 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     6001 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     6061 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     6121 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     6181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     6241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     6301 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     6361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     6421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     6481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     6541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     6601 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     6661 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     6721 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     6781 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     6841 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     6901 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     6961 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     7021 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     7081 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     7141 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     7201 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     7261 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//

