LOCUS       pEXPR-myr-FLAG-hTRIM31.2        6827 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10369.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10369
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214254|
COMMENT     VNTDBDATE|-11264403|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM31.2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(5831..6691)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3530..4324
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4500..4574
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4500..4574)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4888..5290)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4713..4796)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3363..3438
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2692..3120
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2422..2646
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3125..3406
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2358
                     /vntifkey="4"
                     /label=hTRIM31
     attenuator      2359..2382
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1669 a      1735 c      1735 g      1688 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggccagt gggcagtttg tgaacaaact gcaagaggaa gtgatctgcc ccatctgcct 
     1141 ggacattctg cagaaacctg tcaccatcga ctgtgggcac aatttctgcc tcaaatgcat 
     1201 cactcagatt ggggaaacat catgtggatt tttcaaatgt cccctctgca aaacttccgt 
     1261 aaggaagaac gcaatcaggt tcaactcgct gttgcggaat ctggtggaga aaatccaagc 
     1321 tctacaagcc tctgaggtgc agtccaaaag gaaagaggct acatgcccga ggcaccagga 
     1381 gatgttccac tatttctgcg aggatgatgg gaagttcctc tgttttgtgt gtcgtgaatc 
     1441 caaggaccac aaatcccata atgtcagctt gatcgaagaa gctgcccaga attatcaggg 
     1501 gcagattcaa gagcagatcc aagtcttgca gcaaaaggag aaggagacag tacaagtgaa 
     1561 ggcacaaggt gtacacaggg tcgatgtctt cacggaccag gtagaacatg agaagcaaag 
     1621 gatcctcaca gaatttgaac tcctgcatca agtcctagag gaggagaaga atttcctgct 
     1681 atcacggatt tactggctgg gtcatgaggg aacggaagcg gggaaacact atgttgcctc 
     1741 cactgagcca cagttgaacg atctcaagaa gctcgttgat tccctgaaga ccaagcagaa 
     1801 catgccaccc aggcagctgc tggaggatat caaagtcgtc ttgtgcagaa gtgaagagtt 
     1861 tcagtttctc aacccaaccc ctgttcctct ggaactggag aaaaaactca gtgaagcaaa 
     1921 atcaagacac gactccatca cagggagcct gaaaaaattc aaagaccaac tccaggctga 
     1981 taggaaaaaa gatgaaaaca gattcttcaa aagcatgaat aaaaatgaca tgaagagctg 
     2041 gggcttgtta cagaaaaata atcataaaat gaacaaaacc tcagagcccg ggtcatcttc 
     2101 tgcaggcggc agaactacat cggggccacc aaatcaccac tcttcagccc catcccactc 
     2161 cctgtttcgg gcctcgtctg ctgggaaagt cacttttcca gtatgtctcc tggcctctta 
     2221 tgatgagatt tctggtcaag gagcgagctc tcaggatacg aagacatttg acgttgcgct 
     2281 gtccgaggag ctccatgcgg cactgagtga gtggctgaca gcgatccggg cttggttttg 
     2341 taaggttcct tcaagctacc caactttctt gtacaaagtg gtgatctaga gggcccgttt 
     2401 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 
     2461 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 
     2521 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 
     2581 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 
     2641 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct 
     2701 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     2761 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     2821 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     2881 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     2941 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     3001 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     3061 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     3121 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 
     3181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 
     3241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 
     3301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     3361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 
     3421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 
     3481 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 
     3541 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 
     3601 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 
     3661 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 
     3721 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 
     3781 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 
     3841 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 
     3901 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 
     3961 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 
     4021 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 
     4081 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 
     4141 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 
     4201 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 
     4261 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 
     4321 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac 
     4381 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 
     4441 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 
     4501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     4561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     4621 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     4681 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     4741 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     4801 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     4861 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     4921 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     4981 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     5041 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     5101 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     5161 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     5221 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     5281 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     5341 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     5401 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     5461 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     5521 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     5581 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     5641 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     5701 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     5761 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     5821 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     5881 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     5941 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     6001 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     6061 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     6121 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     6181 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     6241 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     6301 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     6361 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     6421 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     6481 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     6541 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     6601 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     6661 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     6721 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     6781 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//

