LOCUS       pEXPR-myr-FLAG-hTRIM37        8444 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10370.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10370
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214260|
COMMENT     VNTDBDATE|-11264410|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM37|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM37'" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(7448..8308)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             5147..5941
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      6117..6191
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(6117..6191)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(6505..6907)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(6330..6413)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      4980..5055
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      4309..4737
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      4039..4263
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        4742..5023
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..3975
                     /vntifkey="4"
                     /label=hTRIM37
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      3976..3999
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     2171 a      2001 c      2122 g      2150 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggatgaa cagagcgtgg agagcattgc tgaggttttc cgatgtttca tttgtatgga 
     1141 gaaattgcgg gatgcacgcc tgtgtcctca ttgctccaaa ctgtgttgtt tcagctgtat 
     1201 taggcgctgg ctgacagagc agagagctca atgtcctcat tgccgtgctc cactccagct 
     1261 acgagaacta gtaaattgtc gttgggcaga agaagtaaca caacagcttg atactcttca 
     1321 actctgcagt ctcaccaaac atgaagaaaa tgaaaaggac aaatgtgaaa atcaccatga 
     1381 aaaacttagt gtattttgct gggcttgtaa gaagtgtatc tgccatcagt gtgcactttg 
     1441 gggaggaatg catggcggac atacctttaa acctttggca gaaatttatg agcaacacgt 
     1501 cactaaagtg aatgaagagg tagccaaact tcgtcggcgt ctcatggaac tgatcagctt 
     1561 agttcaagaa gtggaaagga atgtagaagc tgtaagaaat gcaaaagatg agcgtgttcg 
     1621 ggaaattagg aatgcagtgg agatgatgat tgcacggtta gacacacagc tgaagaataa 
     1681 gcttataaca ctgatgggtc agaagacatc tctaacccaa gaaacagagc ttttggaatc 
     1741 cttacttcag gaggtggagc accagttgcg gtcttgtagt aagagtgagt tgatatctaa 
     1801 gagctcagag atccttatga tgtttcagca agttcatcgg aagcccatgg catcttttgt 
     1861 taccactcct gttccaccag actttaccag tgaattagtg ccatcttacg attcagctac 
     1921 ttttgtttta gagaatttca gcactttgcg tcagagagca gatcctgttt acagtccacc 
     1981 tcttcaagtt tcaggacttt gctggaggtt aaaagtttac ccagatggaa atggagttgt 
     2041 gcgaggttac tacttatctg tgtttctgga gctctcagct ggcttgcctg aaacttctaa 
     2101 atatgaatat cgtgtagaga tggttcacca gtcctgtaat gatcctacaa aaaatatcat 
     2161 tcgagaattt gcatctgact ttgaagttgg agaatgctgg ggctataata gatttttccg 
     2221 tttggactta ctcgcaaatg aaggatactt gaatccacaa aatgatacag tgattttaag 
     2281 gtttcaggta cgttcaccaa ctttctttca aaaatcccgg gaccagcatt ggtacattac 
     2341 tcagctggaa gctgcacaga ctagttatat ccaacaaata aacaacctta aagagagact 
     2401 tactattgag ctgtctcgaa ctcagaagtc aagagatttg tcaccaccag ataaccatct 
     2461 tagcccccaa aatgatgatg ctctggagac acgagctaag aagtctgcat gctctgacat 
     2521 gcttctcgaa ggtggtccta ctacagcttc tgtaagagag gccaaagagg atgaagaaga 
     2581 tgaggagaag attcagaatg aagattatca tcacgagctt tcagatggag atctggatct 
     2641 ggatcttgtt tatgaggatg aagtaaatca gctcgatggc agcagttcct ctgctagttc 
     2701 cacagcaaca agtaatacag aagaaaatga tattgatgaa gaaactatgt ctggagaaaa 
     2761 tgatgtggaa tataacaaca tggaattaga agagggagaa ctcatggaag atgcagctgc 
     2821 tgcaggaccc gcaggtagta gccatggtta tgtgggttcc agtagtagaa tatcaagaag 
     2881 aacacattta tgctccgctg ctaccagtag tttactagac attgatccat taattttaat 
     2941 acatttgttg gaccttaagg accggagcag tatagaaaat ttgtggggct tacagcctcg 
     3001 cccacctgct tcacttctgc agcccacagc atcatattct cgaaaagata aagaccaaag 
     3061 gaagcaacag gcaatgtggc gagtgccctc tgatttaaag atgctaaaaa gactcaaaac 
     3121 tcaaatggcc gaagttcgat gtatgaaaac tgatgtaaag aatacacttt cagaaataaa 
     3181 aagcagcagt gctgcttctg gagacatgca gacaagcctt ttttctgctg accaggcagc 
     3241 tctggctgca tgtggaactg aaaactctgg cagattgcag gatttgggaa tggaactcct 
     3301 ggcaaagtca tcagttgcca attgttacat acgaaactcc acaaataaga agagtaattc 
     3361 gcccaagcca gctcgatcca gtgtagcagg tagtctatca cttcgaagag cagtggaccc 
     3421 tggagaaaat agtcgttcaa agggagactg tcagactctg tctgaaggct ccccaggaag 
     3481 ctctcagtct gggagcaggc acagttctcc ccgagccttg atacatggca gtatcggtga 
     3541 tattctgcca aaaactgaag accggcagtg taaagctttg gattcagatg ctgttgtggt 
     3601 tgcagttttc agtggcttgc ctgcggttga gaaaaggagg aaaatggtca ccttgggggc 
     3661 taatgctaaa ggaggtcatc tggaaggact gcagatgact gatttggaaa ataattctga 
     3721 aactggagag ttacagcctg tactacctga aggagcttca gctgcccctg aagaaggaat 
     3781 gagtagcgac agtgacattg aatgtgacac tgagaatgag gagcaggaag agcataccag 
     3841 tgtgggcggg tttcacgact ccttcatggt catgacacag cccccggatg aagatacaca 
     3901 ttccagtttt cctgatggtg aacaaatagg ccctgaagat ctcagcttca atacagatga 
     3961 aaatagtgga agatacccaa ctttcttgta caaagtggtg atctagaggg cccgtttaaa 
     4021 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     4081 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     4141 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     4201 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 
     4261 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 
     4321 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     4381 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     4441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     4501 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     4561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     4621 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 
     4681 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 
     4741 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     4801 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     4861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     4921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     4981 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
     5041 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 
     5101 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     5161 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     5221 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     5281 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
     5341 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     5401 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     5461 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     5521 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     5581 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     5641 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 
     5701 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     5761 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     5821 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     5881 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     5941 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 
     6001 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 
     6061 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 
     6121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     6181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     6241 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 
     6301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     6361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     6421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     6481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     6541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     6601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     6661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     6721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     6781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     6841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     6901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     6961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     7021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     7081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     7141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     7201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     7261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     7321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     7381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     7441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     7501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     7561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     7621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     7681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     7741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     7801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     7861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     7921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     7981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     8041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     8101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     8161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     8221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     8281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     8341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     8401 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc 
//

