LOCUS       pEXPR-myr-FLAG-hTRIM43        6890 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10372.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10372
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214266|
COMMENT     VNTDBDATE|-11264443|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM43|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM43'" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5894..6754)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3593..4387
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4563..4637
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4563..4637)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4951..5353)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4776..4859)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3426..3501
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2755..3183
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2485..2709
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3188..3469
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2421
                     /vntifkey="4"
                     /label=hTRIM43
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      2422..2445
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1667 a      1729 c      1745 g      1749 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggactca gacttctcac atgccttcca gaaggaactc acctgcgtca tctgtttgaa 
     1141 ctacctggta gaccctgtca ccatctgctg tgggcacagc ttctgtaggc cctgtctctg 
     1201 cctttcgtgg gaggaagccc aaagtcctgc aaactgccct gcatgcaggg aaccatcacc 
     1261 gaaaatggac ttcaaaacca atattcttct gaagaattta gtgaccattg ccagaaaagc 
     1321 cagtctctgg caattcctga gctctgagaa acaaatatgt gggacccata ggcaaacaaa 
     1381 gaagatgttc tgtgacatgg acaagagtct cctctgcttg ctgtgctcca actctcagga 
     1441 gcacggggct cacaaacacc atcccatcga agaggcagct gaggaacacc gggagaaact 
     1501 cttaaagcaa atgaggattt tatggaaaaa gattcaagaa aatcagagaa atctatatga 
     1561 ggagggaaga acagccttcc tctggagggg caatgtggtt ttacgggcac agatgatcag 
     1621 gaatgagtat aggaagctgc atccggttct ccataaggaa gaaaaacaac atttagagag 
     1681 actgaacaag gaataccaag agatttttca gcaactccag agaagttggg tcaaaatgga 
     1741 tcaaaagagt aaacacttga aagaaatgta tcaggaacta atggaaatgt gtcataaacc 
     1801 agatgtggag ctgctccagg atttgggaga catcgtggca aggagtgagt ccgtgctgct 
     1861 gcacatgccc cagcctgtga atccagagct cactgcagga cccatcactg gactggtgta 
     1921 caggctcaac cgcttccgag tggaaatttc cttccatttt gaagtaacca atcacaatat 
     1981 caggctcttt gaggatgtga gaagttggat gtttagacgt ggacctttga attctgacag 
     2041 atctgactat tttgctgcat ggggagccag ggtcttctcc tttgggaaac actactggga 
     2101 gctggatgtg gacaactctt gtgactgggc tctgggagtc tgtaacaact cctggataag 
     2161 gaagaatagc acaatggtta actctgagga catatttctt cttttgtgtc tgaaggtgga 
     2221 taatcatttc aatctcttga ccacctcccc agtgtttcct cactacatag agaaacctct 
     2281 gggccgggtt ggtgtgtttc ttgattttga aagtggaagt gtgagttttt tgaatgtcac 
     2341 caagagttcc ctcatatgga gttacccagc tggctcctta acttttcctg tcaggccttt 
     2401 cttttacact ggccacagat gcccaacttt cttgtacaaa gtggtgatct agagggcccg 
     2461 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 
     2521 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 
     2581 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 
     2641 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 
     2701 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 
     2761 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     2821 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     2881 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     2941 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
     3001 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
     3061 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
     3121 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
     3181 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
     3241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
     3301 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     3361 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     3421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     3481 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     3541 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 
     3601 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 
     3661 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 
     3721 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 
     3781 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 
     3841 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 
     3901 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 
     3961 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 
     4021 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 
     4081 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 
     4141 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 
     4201 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 
     4261 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 
     4321 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 
     4381 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat 
     4441 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 
     4501 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 
     4561 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     4621 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     4681 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 
     4741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 
     4801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 
     4861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 
     4921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 
     4981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 
     5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
     5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     5401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     5461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     5521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 
     5581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     5641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     5701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     5761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     5821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     5881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     5941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     6001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     6061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     6121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     6181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     6241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     6301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     6361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     6421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     6481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     6541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     6601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     6661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     6721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     6781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     6841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
//

