LOCUS pEXPR-myr-FLAG-hTRIM48 6176 bp DNA circular 2-JUN-2016 DEFINITION LMBP 10373. SOURCE ORGANISM COMMENT Accession number: LMBP 10373 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11214269| COMMENT VNTDBDATE|-11264448| COMMENT LSOWNER| COMMENT VNTNAME|pEXPR-myr-FLAG-hTRIM48| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#212=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -743 100) COMMENT (CObjectList) (CObjectList)) COMMENT #213=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -657 100) COMMENT (CObjectList) (CObjectList)) COMMENT #214=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -953 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) -2147483648 COMMENT (CStringList) 2147483648 268632118 (CObList))) FEATURES Location/Qualifiers CDS complement(5180..6040) /vntifkey="4" /label=ampicillin\resistance CDS 2879..3673 /vntifkey="4" /label=Tn5\neomycin\resistance terminator 3849..3923 /vntifkey="43" /label=SV40\polyA\early terminator complement(3849..3923) /vntifkey="43" /label=SV40\polyA\late rep_origin complement(4237..4639) /vntifkey="33" /label=pMB1\ori promoter complement(4062..4145) /vntifkey="30" /label=lac\promoter promoter 235..818 /vntifkey="29" /label=hCMV-IE\promoter\and\enhancer rep_origin 2712..2787 /vntifkey="33" /label=SV40\ori rep_origin 2041..2469 /vntifkey="33" /label=f1\ori promoter 863..879 /vntifkey="30" /label=T7\promoter misc_marker 968..991 /vntifkey="22" /label=FLAG\epitope terminator 1771..1995 /vntifkey="43" /label=BGH\polyA promoter 2474..2755 /vntifkey="29" /label=SV40\early\promoter CDS 914..952 /vntifkey="4" /label=mLck\myristoylation-targeting\sequence CDS 1082..1707 /vntifkey="4" /label=hTRIM48 attenuator 1058..1081 /vntifkey="0" /label=attB1 attenuator 1708..1731 /vntifkey="0" /label=attB2 BASE COUNT 1465 a 1588 c 1573 g 1550 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 1081 catgaattct ggaatctcgc aagtcttcca gagggaactc acctgcccca tctgcatgaa 1141 ctacttcata gacccggtca ccatagactg tgggcacagc ttttgcaggc cctgtttcta 1201 cctcaactgg caagacatcc caattcttac tcagtgcttt gaatgcataa agacaataca 1261 gcagagaaac ctcaaaacta acattcgatt gaagaagatg gcttcccttg ccagaaaagc 1321 cagtctctgg ctattcctga gctctgagga gcaaatgtgt ggcattcaca gggagacaaa 1381 gaagatgttc tgtgaagtgg acaggagcct gctctgtttg ctgtgctcca gctctcagga 1441 gcaccggtat cacagacact gtcccgctga gtgggctgct gaggaacact gggagaagct 1501 gttaaagaaa atgcagtctt tatgggaaaa agcttgtgaa aatcagagaa acctgaatgt 1561 ggaaaccacc agaatcagcc actggaaggc ttttggagac atattataca ggagtgagtc 1621 cgtgctgctg cacatgcccc agcctctgaa tctagcgctc agggcagggc ccatcactgg 1681 actgagggac aggctcaacc aattctgccc aactttcttg tacaaagtgg tgatctagag 1741 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 1801 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 1861 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 1921 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 1981 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 2041 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 2101 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 2161 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 2221 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 2281 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 2341 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 2401 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 2461 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 2521 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 2581 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 2641 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 2701 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 2761 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 2821 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 2881 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 2941 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 3001 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 3061 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 3121 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 3181 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 3241 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 3301 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 3361 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 3421 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 3481 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 3541 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 3601 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 3661 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 3721 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 3781 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 3841 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 3901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 3961 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 4021 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 4081 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 4141 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 4201 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 4261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 4321 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 4381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 4441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 4501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 4561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 4621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 4861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 4981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 5041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 5101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 5161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 5461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 5521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 5581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 5701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 5821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 5941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //