LOCUS       pEXPR-myr-FLAG-hTRIM49        6911 bp    DNA     circular      9-JUN-2016
DEFINITION  LMBP 10374.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10374
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214270|
COMMENT     VNTDBDATE|-11274456|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM49|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM49'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 03:45" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5915..6775)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3614..4408
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4584..4658
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4584..4658)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4972..5374)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4797..4880)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3447..3522
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2776..3204
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2506..2730
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3209..3490
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1085..2442
                     /vntifkey="4"
                     /label=hTRIM49
     attenuator      2443..2466
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1681 a      1725 c      1735 g      1770 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 caccatgaat tctggaatct tacaggtctt tcagggggaa ctcatctgcc ccctgtgcat 
     1141 gaactacttc atagacccgg tcaccataga ctgtgggcac agcttttgca ggccttgttt 
     1201 ctacctcaac tggcaagaca tcccatttct tgtccagtgc tctgaatgca caaagtcaac 
     1261 cgagcagata aacctcaaaa ccaacattca tttgaagaag atggcttctc ttgccagaaa 
     1321 agtcagtctc tggctattcc tgagctctga ggagcaaatg tgtggcactc acagggagac 
     1381 aaagaagata ttctgtgaag tggacaggag cctgctctgt ttgctgtgct ccagctctca 
     1441 ggagcaccgg tatcacagac accgtcccat tgagtgggct gctgaggaac accgggagaa 
     1501 gcttttacag aaaatgcagt ctttgtggga aaaagcttgt gaaaatcaca gaaacctgaa 
     1561 tgtggaaacc accagaacca gatgctggaa ggattatgtg aatttaaggc tagaagcaat 
     1621 tagagctgag tatcagaaga tgcctgcatt tcatcatgaa gaagaaaaac ataatttgga 
     1681 gatgctgaaa aagaagggga aagaaatttt tcatcgactt catttaagta aagccaaaat 
     1741 ggctcatagg atggagattt taagaggaat gtatgaggag ctgaacgaaa tgtgccataa 
     1801 accagatgtg gagctacttc aggcttttgg agacatatta cacaggagtg agtccgtgct 
     1861 gctgcacatg ccccagcctc tgaatccaga gctcagtgca gggcccatca ctggactgag 
     1921 ggacaggctc aaccaattcc gagtgcatat tactctgcat catgaagaag ccaacaatga 
     1981 tatctttctg tatgaaattt tgagaagcat gtgtattgga tgtgaccatc aagatgtacc 
     2041 ctatttcact gcaacaccta gaagttttct tgcatggggt gttcagactt tcacctcggg 
     2101 caaatattac tgggaggtcc atgtagggga ctcctggaat tgggcttttg gtgtctgtaa 
     2161 tatgtatcgg aaagagaaga atcagaatga gaagatagat ggaaaggcgg gactctttct 
     2221 tcttgggtgt gttaagaatg acattcaatg cagtctcttt accacctccc cacttatgct 
     2281 gcaatatatc ccaaaaccta ccagccgagt aggattattc ctggattgtg aggctaagac 
     2341 tgtgagcttt gttgatgtta atcaaagctc cctaatatac accatcccta attgctcttt 
     2401 ctcacctcct ctcaggccta tcttttgctg tattcacttc ttgccaactt tcttgtacaa 
     2461 agtggtgatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt 
     2521 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 
     2581 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 
     2641 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 
     2701 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 
     2761 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     2821 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     2881 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
     2941 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     3001 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     3061 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     3121 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
     3181 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 
     3241 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3301 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     3361 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     3421 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     3481 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     3541 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 
     3601 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 
     3661 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 
     3721 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 
     3781 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 
     3841 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 
     3901 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 
     3961 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 
     4021 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 
     4081 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 
     4141 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 
     4201 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 
     4261 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 
     4321 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 
     4381 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc 
     4441 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 
     4501 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 
     4561 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 
     4621 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     4681 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 
     4741 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 
     4801 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 
     4861 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 
     4921 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 
     4981 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     5041 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     5101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     5161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     5221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     5281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     5341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     5401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     5461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     5521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     5581 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     5641 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     5701 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     5761 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     5821 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     5881 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     5941 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     6001 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     6061 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     6121 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     6181 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     6241 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     6301 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     6361 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     6421 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     6481 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     6541 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     6601 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     6661 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     6721 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     6781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     6841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     6901 cacctgacgt c 
//

