LOCUS       pEXPR-myr-FLAG-hTRIM50        7016 bp    DNA     circular     22-JUN-2016
DEFINITION  LMBP 10375.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10375
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214310|
COMMENT     VNTDBDATE|-11293384|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM50|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM50'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 04:25" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6020..6880)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3719..4513
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4689..4763
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4689..4763)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5077..5479)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4902..4985)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3552..3627
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2881..3309
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2611..2835
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3314..3595
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1085..2547
                     /vntifkey="4"
                     /label=hTRIM50
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      2548..2571
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1553 a      1941 c      1871 g      1651 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 caccatggct tggcaggtga gcctgccaga gctggaggac cggcttcagt gtcccatctg 
     1141 cctggaggtc ttcaaggagc ccctgatgct gcagtgtggc cactcttact gcaagggctg 
     1201 cctggtttcc ctgtcctgcc acctggatgc cgagctgcgc tgccccgtgt gccggcaggc 
     1261 ggtggacggc agcagctccc tgcccaacgt ctccctggcc agggtgatcg aagccctgag 
     1321 gctccctggg gacccggagc ccaaggtctg cgtgcaccac cggaacccgc tcagcctttt 
     1381 ctgcgagaag gaccaggagc tcatctgtgg cctctgcggt ctgctgggct cccaccaaca 
     1441 ccacccggtc acgcccgtct ccaccgtcta cagccgcatg aaggaggagc tcgcagccct 
     1501 catctctgag ctgaagcagg agcagaaaaa ggtggatgag ctcatcgcca aactggtgaa 
     1561 caaccggacc cgaatcgtca atgagtcgga tgtcttcagc tgggtgatcc gccgcgagtt 
     1621 ccaggagctg caccacctgg tggatgagga gaaggcccgc tgcctggagg ggataggggg 
     1681 tcacacccgt ggcctggtgg cctccctgga catgcagctg gagcaggccc agggaacccg 
     1741 ggagcggctg gcccaagccg agtgtgtgct ggaacagttc ggcaatgagg accaccacaa 
     1801 gttcatccgg aagttccact ccatggcctc cagagcagag atgccgcagg cccggccctt 
     1861 agaaggcgca ttcagcccca tctccttcaa gccaggcctc caccaggctg acatcaagct 
     1921 gaccgtgtgg aaaaggctct tccggaaagt tttgccagcc ccggagcctc tcaagttgga 
     1981 ccctgccact gcccacccac tcctggagct ctccaagggc aacacggtgg tgcagtgcgg 
     2041 gcttctggcc cagcggcgag ccagccagcc tgagcgcttc gactacagca cctgcgtcct 
     2101 ggccagccgc ggcttctcct gcggccgcca ctactgggag gtggtggtgg gcagcaagag 
     2161 cgactggcgc ctgggggtca tcaagggcac agccagccgt aagggcaagc tgaacaggtc 
     2221 ccccgagcac ggcgtgtggc tgatcggcct gaaggagggc cgggtgtacg aagcctttgc 
     2281 ctgcccccgg gtacccctgc ccgtggccgg ccacccccac cgcatcgggc tctacctgca 
     2341 ctatgagcag ggcgaactca ccttcttcga tgccgaccgc cccgatgacc tgcggccgct 
     2401 ctacaccttc caggccgact tccagggcaa gctctacccc atcctggaca cctgctggca 
     2461 cgagaggggc agcaactcgc tgcccatggt gctgcccccg cccagcgggc ctggccccct 
     2521 cagccccgag cagcccacca agctgttgcc aactttcttg tacaaagtgg tgatctagag 
     2581 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     2641 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     2701 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     2761 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     2821 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     2881 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     2941 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     3001 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     3061 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     3121 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     3181 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     3241 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     3301 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     3361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     3421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     3481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     3541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     3601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     3661 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     3721 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     3781 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     3841 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     3901 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     3961 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     4021 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     4081 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     4141 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     4201 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     4261 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     4321 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     4381 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     4441 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     4501 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 
     4561 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     4621 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     4681 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     4741 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     4801 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     4861 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     4921 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     4981 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     5041 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     5101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     5161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     5221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     5281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     5341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     5401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     5461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     5521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     5581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     5641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     5701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     5761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     5821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     5881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     5941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     6001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     6061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     6121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     6181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     6241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     6301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     6361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     6421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     6481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     6541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     6601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     6661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     6721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     6781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     6841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     6901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     6961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//

