LOCUS       pEXPR-myr-FLAG-hTRIM65        5912 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10377.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10377
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214321|
COMMENT     VNTDBDATE|-11264541|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM65|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM65'" 1) 
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COMMENT                          "Created: 04/28/16 04:36" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(4916..5776)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             2615..3409
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      3585..3659
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3585..3659)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(3973..4375)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(3798..3881)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      2448..2523
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      1777..2205
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      1507..1731
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2210..2491
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..1443
                     /vntifkey="4"
                     /label=hTRIM65
     attenuator      1444..1467
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1348 a      1571 c      1524 g      1469 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catgtgccca gagcttccag gccgggcacc actactggga ggtgcgcgcg tcagaccact 
     1141 cggtgacact gggcgtctcc tacccgcaac tgccacggtg caggctgggg ccccacacag 
     1201 acaacattgg ccggggaccc tgctcctggg ggctctgcgt ccaggaggac agcctccagg 
     1261 cctggcacaa cggggaagcc cagcgcctcc caggggtgtc agggcggctc ctgggcatgg 
     1321 atttggacct ggcctcaggc tgcctcacct tctacagcct ggagccccag acccagcccc 
     1381 tgtacacctt ccatgccctc ttcaaccagc ccctcacccc cgtcttctgg ctcctcgagg 
     1441 gtacccaact ttcttgtaca aagtggtgat ctagagggcc cgtttaaacc cgctgatcag 
     1501 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     1561 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     1621 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     1681 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 
     1741 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 
     1801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     1861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     1921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     1981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     2041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     2101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
     2161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 
     2221 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     2281 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 
     2341 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     2401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     2461 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 
     2521 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 
     2581 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 
     2641 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 
     2701 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 
     2761 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 
     2821 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 
     2881 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 
     2941 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 
     3001 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 
     3061 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 
     3121 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 
     3181 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 
     3241 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 
     3301 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 
     3361 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 
     3421 gggttcgcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 
     3481 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 
     3541 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 
     3721 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 
     3781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 
     3841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     3901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     3961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     4021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     4081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     4141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     4201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     4261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     4321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     4381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     4441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     4501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     4561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 
     4621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     4681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     4741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     4801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     4861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     4921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     4981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     5041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     5101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     5161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     5221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     5281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     5341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     5401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     5461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     5521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     5581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     5641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     5701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     5761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     5821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     5881 gcacatttcc ccgaaaagtg ccacctgacg tc 
//

