LOCUS       pEXPR-myr-FLAG-hTRIM69        6575 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10378.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10378
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214323|
COMMENT     VNTDBDATE|-11264543|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM69|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM69'" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5579..6439)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3278..4072
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4248..4322
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4248..4322)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4636..5038)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4461..4544)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3111..3186
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2440..2868
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2170..2394
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2873..3154
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..2106
                     /vntifkey="4"
                     /label=hTRIM69
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      2107..2130
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1603 a      1667 c      1663 g      1642 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggaggag gagcttgcca tccaacaggg tcaactggag acaactctga aggagcttca 
     1141 gaccctgagg aacatgcaga aggaagctat tgctgctcac aaggaaaaca agctacatct 
     1201 gcagcaacat gtgtccatgg agtttctaaa gctgcatcag ttcctgcaca gcaaagaaaa 
     1261 ggacatttta actgagctcc gggaagaggg gaaagccttg aatgaggaga tggagttgaa 
     1321 tctgagccag cttcaggagc aatgtctctt agccaaggat atgttggtga gcattcaggc 
     1381 aaagacggaa caacagaact ccttcgactt tctcaaagac atcacaactc tcttacatag 
     1441 cttggagcaa ggaatgaagg tgctggcaac cagagagctt atttccagaa agctgaacct 
     1501 gggccagtac aaaggtccta tccagtacat ggtatggagg gaaatgcagg acactctctg 
     1561 cccaggcctg tctccactaa ctctggaccc taaaacagct cacccaaatc tggtgctctc 
     1621 caaaagccaa accagcgtct ggcatggtga cattaagaag ataatgcctg atgatcctga 
     1681 gaggtttgac tcaagtgtgg ctgtactggg ctcaagaggc ttcacctctg gaaagtggta 
     1741 ctgggaagta gaagtagcaa agaagacaaa atggacagtt ggagttgtca gagaatccat 
     1801 cattcggaag ggcagctgtc ctctaactcc tgagcaagga ttctggcttt taagactaag 
     1861 gaaccaaact gatctaaagg ctctggattt gccttctttc agtctgacac tgactaacaa 
     1921 cctcgacaag gtgggcatat acctggatta tgaaggagga cagttgtcct tctacaatgc 
     1981 taaaaccatg actcacattt acaccttcag taacactttc atggagaaac tttatcccta 
     2041 cttctgcccc tgccttaatg atggtggaga gaataaagaa ccattgcaca tcttacatcc 
     2101 acagtaccca actttcttgt acaaagtggt gatctagagg gcccgtttaa acccgctgat 
     2161 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 
     2221 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 
     2281 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 
     2341 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 
     2401 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat 
     2461 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 
     2521 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 
     2581 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 
     2641 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 
     2701 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 
     2761 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 
     2821 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa 
     2881 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     2941 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     3001 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     3061 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     3121 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 
     3181 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 
     3241 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 
     3301 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 
     3361 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 
     3421 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 
     3481 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 
     3541 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 
     3601 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 
     3661 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 
     3721 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 
     3781 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 
     3841 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 
     3901 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 
     3961 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 
     4021 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 
     4081 ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 
     4141 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 
     4201 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 
     4261 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     4321 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 
     4381 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 
     4441 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     4501 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     4561 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     4621 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     4681 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     4741 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     4801 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     4861 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     4921 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     4981 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     5041 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     5101 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     5161 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     5221 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 
     5281 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     5341 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     5401 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     5461 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     5521 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     5581 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     5641 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     5701 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     5761 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     5821 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     5881 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     5941 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     6001 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     6061 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     6121 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     6181 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     6241 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     6301 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     6361 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     6421 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     6481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     6541 cgcgcacatt tccccgaaaa gtgccacctg acgtc 
//

