LOCUS       pDG2i-mRipk3-Flag puro        9712 bp    DNA     circular UNA 18-JUN-2018
DEFINITION  LMBP 10386.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10386
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 18-JUN-2018
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12338795|
COMMENT     VNTDBDATE|-12338795|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDG2i-mRipk3-Flag puro|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDG2i-mRipk3-Flag puro" 0 9712 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 80) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HindIII" "NdeI" "PvuII" "SpeI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1336 1335 1 9712 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 6 223 0 3567 0 3879 0 
COMMENT            4435 0 5019 0 8760 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 8351 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 53 0 829 0 3342 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 5674 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 7240 0 7303 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "rtTA3" 4 0 0 7771 8478 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7905..8612") 
COMMENT        #6=(CFSignal (CObList) "5' UTR of the EMCV polyprotein gene (IRES)" 32 0 
COMMENT            0 8535 9107 0 (CStringList) (CStringList) 1 1 1 1 "8669..9241") 
COMMENT        #7=(CFSignal (CObList) "puromycin resistance" 4 0 0 9110 9712 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "9244..9846") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 0 397 471 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "397..471") 
COMMENT        #9=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 3653 3981 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3653..3981") 
COMMENT        #10=(CFSignal (CObList) "f1 origin" 33 0 0 900 1355 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "900..1355") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 0 1486 2346 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1486..2346") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 0 2887 3289 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2887..3289") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 0 3381 3464 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3381..3464") 
COMMENT        #14=(CFSignal (CObList) "T3 promoter" 30 0 0 3528 3544 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3528..3544") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 397 471 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(397..471)") 
COMMENT        #16=(CFSignal (CObList) "SV40 ori" 33 0 0 607 682 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "607..682") 
COMMENT        #17=(CFSignal (CObList) "T7 promoter" 30 0 1 716 732 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(716..732)") 
COMMENT        #18=(CFSignal (CObList) "HIV-1 psi packaging signal" 21 0 0 4092 4136 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4092..4136") 
COMMENT        #19=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 4646 4879 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4646..4879") 
COMMENT        #20=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 91 325 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "91..325") 
COMMENT        #21=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 75 90 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "75..90") 
COMMENT        #22=(CFSignal (CObList) "hCMVTRE promoter" 29 0 0 5397 5664 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5397..5664") 
COMMENT        #23=(CFSignal (CObList) "SV40 early promoter" 29 0 0 7380 7662 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7514..7796") 
COMMENT        #24=(CFSignal (CObList) "SV40 ori" 33 0 0 7619 7694 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7753..7828") 
COMMENT        #25=(CFSignal (CObList) "loxP" 21 0 0 8479 8512 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8613..8646") 
COMMENT        #26=(CFSignal (CObList) "FLAG epitope" 22 0 0 7321 7344 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7455..7478") 
COMMENT        #27=(CFSignal (CObList) "attB1" 0 0 0 5707 5731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8158..8178") 
COMMENT        #28=(CFSignal (CObList) "mRipk3" 4 0 0 5779 7236 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "8229..9686") 
COMMENT        #29=(CFSignal (CObList) "attB2" 0 0 1 7253 7277 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #30=(CFSignal (CObList) "Last modified: 18-JUN-2018" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #31=(CFSignal (CObList) "LMBP 10386" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #32=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #33=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pDG2i-mRipk3-Flag puro" 2) 5 "" 0 4) 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pDG2i-mRipk3-Flag puro'" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 10386." 
COMMENT                          1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/18/18 01:50" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/18/18 01:50" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9712 bp" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: UNA" 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 21-JUN-2016" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #47=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Ghent University" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Technologiepark 927" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "B-9052 Gent-Zwijnaarde Belgium" 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
COMMENT                          1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #55=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #56=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #57=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #58=(CLinePar (CParagraph 0 (15 #30# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 18-JUN-2018") 
COMMENT                     #59=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #60=(CLinePar (CParagraph 0 (15 #31# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 10386"))) "Annotations") 
COMMENT              #61=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5707 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5707  End: 5731" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8158..8178" 1))) "attB1") 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7253 (3 #29# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7253  End: 7277 (Complementary)" 
COMMENT                                        1))) "attB2"))) "Attenuator (2 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1486 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1486  End: 2346" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1486..2346" 1))) 
COMMENT                                 "ampicillin resistance") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5779 (3 #28# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5779  End: 7236" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   8229..9686" 1))) "mRipk3") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7771 (3 #5# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7771  End: 8478" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7905..8612" 1))) "rtTA3") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 9110 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 9110  End: 9712" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   9244..9846" 1))) 
COMMENT                                 "puromycin resistance"))) "CDS (4 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 75 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 75    End: 90  " 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   75..90" 1))) "HIV-1 cPPT") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4092 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4092  End: 4136" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4092..4136" 1))) 
COMMENT                                 "HIV-1 psi packaging signal") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4646 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4646  End: 4879" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4646..4879" 1))) 
COMMENT                                 "HIV-1 Rev response element") 
COMMENT                            #99=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8479 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 8479  End: 8512" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   8613..8646" 1))) "loxP"))) 
COMMENT                          "Misc. Feature (4 total)") 
COMMENT                     #103=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #104=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7321 (3 #26# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7321  End: 7344" 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7455..7478" 1))) "FLAG epitope"))) 
COMMENT                           "Misc. Marker (1 total)") 
COMMENT                     #108=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #109=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3653 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3653  End: 3981" 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3653..3981" 1))) 
COMMENT                                   "RSV/HIV-1 5' LTR") 
COMMENT                             #113=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5397 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5397  End: 5664" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5397..5664" 1))) 
COMMENT                                   "hCMVTRE promoter") 
COMMENT                             #117=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7380 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7380  End: 7662" 1) 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7514..7796" 1))) 
COMMENT                                   "SV40 early promoter"))) 
COMMENT                           "Promoter Eukaryotic (3 total)") 
COMMENT                     #121=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #122=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 716 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 716   End: 732  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(716..732)" 1))) 
COMMENT                                   "T7 promoter") 
COMMENT                             #126=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3381 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3381  End: 3464" 1) 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3381..3464" 1))) "lac promoter") 
COMMENT                             #130=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3528 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3528  End: 3544" 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3528..3544" 1))) "T3 promoter"))) 
COMMENT                           "Promoter Prokaryotic (3 total)") 
COMMENT                     #134=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #135=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 8535 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 8535  End: 9107" 1) 
COMMENT                                     #137=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   8669..9241" 1))) 
COMMENT                                   "5' UTR of the EMCV polyprotein gene (IRES)"))) 
COMMENT                           "RBS (1 total)") 
COMMENT                     #139=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #140=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 607 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #141=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 607   End: 682 " 1) 
COMMENT                                     #142=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   607..682" 1))) "SV40 ori") 
COMMENT                             #144=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 900 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #145=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 900   End: 1355" 1) 
COMMENT                                     #146=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   900..1355" 1))) "f1 origin") 
COMMENT                             #148=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2887 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #149=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2887  End: 3289" 1) 
COMMENT                                     #150=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #151=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2887..3289" 1))) "pMB1 ori") 
COMMENT                             #152=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7619 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #153=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7619  End: 7694" 1) 
COMMENT                                     #154=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #155=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7753..7828" 1))) "SV40 ori"))) 
COMMENT                           "Replication Origin (4 total)") 
COMMENT                     #156=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #157=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 91 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #158=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 91    End: 325 " 1) 
COMMENT                                     #159=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #160=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   91..325" 1))) 
COMMENT                                   "RSV/HIV-1 dU3/3' LTR") 
COMMENT                             #161=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 397 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #162=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 397   End: 471 " 1) 
COMMENT                                     #163=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #164=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   397..471" 1))) "SV40 polyA early") 
COMMENT                             #165=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 397 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #166=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 397   End: 471  (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #167=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #168=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(397..471)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #169=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #170=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #171=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #172=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 8351 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
COMMENT                      #173=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #174=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #175=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5674 " 1))))) "SpeI: 1 site") 
COMMENT                            1 5 "ACTAGT" "TGATCA") 
COMMENT                      #176=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #177=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #178=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7240 " 1) 
COMMENT                                      #179=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7303 " 1))))) "XhoI: 2 sites") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
COMMENT                      #180=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #181=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #182=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 53 " 1) 
COMMENT                                      #183=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 829 " 1) 
COMMENT                                      #184=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3342 " 1))))) "PvuII: 3 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #185=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #186=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #187=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 223 " 1) 
COMMENT                                      #188=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3567 " 1) 
COMMENT                                      #189=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3879 " 1) 
COMMENT                                      #190=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4435 " 1) 
COMMENT                                      #191=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5019 " 1) 
COMMENT                                      #192=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 8760 " 1))))) 
COMMENT                             "HindIII: 6 sites") 1 5 "AAGCTT" "TTCGAA"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #193=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #194=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #195=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #196=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #197=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #198=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #199=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #200=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #201=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #202=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #203=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #204=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #205=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #206=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #207=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #208=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #209=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #210=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #211=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #212=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #213=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #214=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #215=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #216=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #217=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #218=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #219=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #220=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #221=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #222=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #223=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #224=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #225=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #226=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #227=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #228=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #229=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #230=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #231=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #232=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #233=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #234=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #235=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #236=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #237=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #238=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #239=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #240=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #241=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #242=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #243=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #244=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #245=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #246=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #247=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #248=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #249=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #250=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #251=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #252=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -9846) 1 1 0 1 1 
COMMENT        (mapper: 28.7155 -22.2206 53.2341 53.2341 0.01 10 -1 9712 9712 1 0 0) 
COMMENT        #253=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 925773360 100) 
COMMENT              (CObjectList 
COMMENT               #254=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -894 100) 
COMMENT                     (CObjectList 
COMMENT                      #255=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #199# 93976044 0) 
COMMENT                             (LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27483 
COMMENT                             6.27283 1 0.0214037) 0.0645921) 
COMMENT                      #256=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #196# 1 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 84 31 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pDG2i-mRipk3-Flag puro" "@N" 1 0 
COMMENT                            0.871165 0 -10 15.1219 1.55915 Nil) 
COMMENT                      #257=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #195# 50338016 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 96 36 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "9712 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.97801 1.82214 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #258=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -894 100) 
COMMENT                     (CObjectList 
COMMENT                      #259=(CGroupWidget 
COMMENT                            (CWidget 0 (7 0 0) 1 2 0 0 Nil -933 100) 
COMMENT                            (CObjectList 
COMMENT                             #260=(CScratch 
COMMENT                                   (CWidget 0 (3 #27# 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 10 8388608) 10 1 2.58756 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #261=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #238# 1413955924 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attB1" "@N" 1 
COMMENT                                   -9.28992 -11.7323 0 -10 2.42326 1.1271 #260#) 
COMMENT                             #262=(CScratch 
COMMENT                                   (CWidget 0 (3 #29# 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 10 8388608) 10 1 1.58897 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #263=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #238# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "attB2" "@N" 1 
COMMENT                                   -16.9702 -0.854454 0 -10 2.42326 1.1271 #262#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #264=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -391 100) 
COMMENT                            (CObjectList 
COMMENT                             #265=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #5# 0) 1 2 0 0 #197# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.797067 1.25438 1 0.082322) 0.8 1.8 0) 
COMMENT                             #266=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# 11529 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rtTA3" "@N" 1 
COMMENT                                   -12.7663 7.36879 0 -10 2.4984 1.1271 #265#) 
COMMENT                             #267=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #197# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    6.27319 0.38949 1 0.082322) 0.8 1.8 0) 
COMMENT                             #268=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "puromycin resistance" "@N" 1 -8.13119 
COMMENT                                   16.3467 0 -10 9.31733 1.1271 #267#) 
COMMENT                             #269=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #197# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.75785 5.31399 1 0.082322) 0.8 1.8 0) 
COMMENT                             #270=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 17.6852 
COMMENT                                   5.28428 0 -10 8.94163 1.1271 #269#) 
COMMENT                             #271=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #28# 0) 1 2 0 0 #197# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1.0015 
COMMENT                                    1.5993 2.54105 1 0.082322) 0.8 1.8 0) 
COMMENT                             #272=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# 1413955924 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mRipk3" 
COMMENT                                   "@N" 1 -15.3909 -7.40873 0 -10 6.63109 1.1271 
COMMENT                                   #271#)) (CObjectList)) 
COMMENT                      #273=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil 539587699 100) 
COMMENT                            (CObjectList 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 3.63072 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #275=(CLabel (CWidget 0 (0 0) 1 2 0 0 #238# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HIV-1 psi packaging signal" "@N" 1 13.2798 
COMMENT                                   -16.1317 0 -10 11.7594 1.1271 #274#) 
COMMENT                             #276=(CLine 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #200# 1386 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 0.835356 3.12174 
COMMENT                                   3.27288) 
COMMENT                             #277=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #238# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "HIV-1 Rev response element" "@N" 1 -5.94286 
COMMENT                                   -17.406 0 -10 12.5108 1.1271 #276#) 
COMMENT                             #278=(CScratch 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 6.22539 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #279=(CLabel (CWidget 0 (0 0) 1 2 0 0 #238# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HIV-1 cPPT" 
COMMENT                                   "@N" 1 -1.95342 20.3394 0 -10 5.241 1.1271 
COMMENT                                   #278#) 
COMMENT                             #280=(CScratch 
COMMENT                                   (CWidget 0 (3 #25# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 0.797067 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #281=(CLabel (CWidget 0 (0 0) 1 2 0 0 #238# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   -11.0934 10.454 0 -10 1.9912 1.1271 #280#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #282=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -1306 100) 
COMMENT                            (CObjectList 
COMMENT                             #283=(CScratch 
COMMENT                                   (CWidget 0 (3 #26# 0) 1 2 0 0 #236# 0 100) 
COMMENT                                   (LOGPEN 0 6 10040064) 6 1 1.53794 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #284=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FLAG epitope" 
COMMENT                                   "@N" 1 -17.8554 2.44379 0 -10 6.31174 1.1271 
COMMENT                                   #283#)) (CObjectList)) 
COMMENT                      #285=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1515 100) 
COMMENT                            (CObjectList 
COMMENT                             #286=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #234# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.989952 3.70177 3.91428 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #287=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/HIV-1 5' LTR" "@N" 1 13.8469 -13.6324 0 
COMMENT                                   -10 7.94603 1.1271 #286#) 
COMMENT                             #288=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #22# 0) 1 2 0 0 #234# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.989165 2.61469 2.7878 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #289=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMVTRE promoter" "@N" 1 -11.0471 -14.7509 0 
COMMENT                                   -10 8.99799 1.1271 #288#) 
COMMENT                             #290=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #23# 0) 1 2 0 0 #234# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.988097 1.32414 1.50693 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #291=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 early promoter" "@N" 1 -19.4983 3.91671 
COMMENT                                   0 -10 9.12948 1.1271 #290#)) (CObjectList)) 
COMMENT                      #292=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -320 100) 
COMMENT                            (CObjectList 
COMMENT                             #293=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #239# 0 100) 
COMMENT                                    (LOGPEN 0 2 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    4.03571 4.08997 1 0.082322) 0.8 1.8 0) 
COMMENT                             #294=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# 1439 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 14.5787 -8.93801 0 -10 5.56034 1.1271 
COMMENT                                   #293#) 
COMMENT                             #295=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #216# 0 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.810603 3.90776 3.99502) 
COMMENT                                   0.4 0.082322 0.935892 1.8 1.8 0) 
COMMENT                             #296=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 promoter" 
COMMENT                                   "@N" 1 14.3138 -10.5052 0 -10 5.42885 1.1271 
COMMENT                                   #295#) 
COMMENT                             #297=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #216# 1538 100) 
COMMENT                                    (LOGPEN 0 6 128) 6 0.835356 5.81135 5.89862) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #298=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 9.24626 13.3874 0 -10 5.42885 1.1271 
COMMENT                                   #297#)) (CObjectList)) 
COMMENT                      #299=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -1515 100) 
COMMENT                            (CObjectList 
COMMENT                             #300=(CLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #214# 1534 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 0.835356 0.390782 
COMMENT                                   0.760895) 
COMMENT                             #301=(CLabel (CWidget 0 (0 0) 1 2 0 0 #238# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of the EMCV polyprotein gene (IRES)" 
COMMENT                                   "@N" 1 -17.6656 13.9386 0 -10 19.4612 1.1271 
COMMENT                                   #300#)) (CObjectList)) 
COMMENT                      #302=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -1304 100) 
COMMENT                            (CObjectList 
COMMENT                             #303=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #209# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    5.39796 5.6925 1 0.082322) 0.8 1.8 0) 
COMMENT                             #304=(CLabel (CWidget 0 (0 0) 1 2 0 0 #237# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 origin" "@N" 
COMMENT                                   1 10.6632 10.6218 0 -10 3.62549 1.1271 #303#) 
COMMENT                             #305=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #209# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.14875 4.40906 1 0.082322) 0.8 1.8 0) 
COMMENT                             #306=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# 45809936 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 14.4979 -6.02593 0 -10 4.1139 1.1271 #305#) 
COMMENT                             #307=(CScratch 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 10 8388608) 10 1 5.88176 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #308=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# 50337284 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 8.01536 14.7503 0 -10 4.00119 1.1271 #307#) 
COMMENT                             #309=(CScratch 
COMMENT                                   (CWidget 0 (3 #24# 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 10 8388608) 10 1 1.35256 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #310=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #237# -858993459 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 -16.6606 5.52755 0 -10 4.00119 1.1271 #309#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #311=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -1656 100) 
COMMENT                            (CObjectList 
COMMENT                             #312=(CScratch 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #200# 1037 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 6.0174 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #313=(CLabel (CWidget 0 (0 0) 1 2 0 0 #247# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 7.8473 17.396 0 -10 
COMMENT                                   7.62669 1.1271 #312#) 
COMMENT                             #314=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #200# 0 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 1 6.0174 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #315=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #247# 7827 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 7.76156 16.0199 0 
COMMENT                                   -10 7.11949 1.1271 #314#) 
COMMENT                             #316=(CLine 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #200# 1372 100) 
COMMENT                                   (LOGPEN 0 8 16711680) 8 0.835356 6.06326 
COMMENT                                   6.21505) 
COMMENT                             #317=(CLabel (CWidget 0 (0 0) 1 2 0 0 #247# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 61 23 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RSV/HIV-1 dU3/3' LTR" "@N" 1 7.46683 20.0219 
COMMENT                                   0 -10 10.0124 1.1271 #316#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #318=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -858 100) 
COMMENT                     (CObjectList 
COMMENT                      #319=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -815 100) 
COMMENT                            (CObjectList 
COMMENT                             #320=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #252# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 0.879744 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #321=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #249# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Nde}I (8351)" "@N (@S)" 5 -12.4968 
COMMENT                                   8.93801 0 -10 4.65867 1.07074 #320#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #322=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -183 100) 
COMMENT                            (CObjectList 
COMMENT                             #323=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #252# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 2.60888 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #324=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #249# 1094918202 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Spe}I (5674)" "@N (@S)" 5 -9.83643 
COMMENT                                   -13.1683 0 -10 4.54596 1.07074 #323#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #325=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -1729 100) 
COMMENT                            (CObjectList 
COMMENT                             #326=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #251# 2082603128 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 1.59736 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #327=(CLabel (CWidget 0 (0 0) 1 2 0 0 #250# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xho}I (7240)" "@N (@S)" 5 -17.9404 
COMMENT                                   -2.70047 0 -10 4.65867 1.07074 #326#) 
COMMENT                             #328=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 1.55667 1.9 0.082322 1) 
COMMENT                             #329=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #250# 1196708931 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xho}I (7303)" "@N (@S)" 5 -17.8274 
COMMENT                                   0.908322 0 -10 4.65867 1.07074 #328#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #330=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #2# 0) 1 2 0 0 Nil -464 100) 
COMMENT                            (CObjectList 
COMMENT                             #331=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #251# 2082603128 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 6.2396 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #332=(CLabel (CWidget 0 (0 0) 1 2 0 0 #250# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (53)" "@N (@S)" 5 -1.93634 
COMMENT                                   18.7662 0 -10 4.03876 1.07074 #331#) 
COMMENT                             #333=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.73836 1.9 0.082322 1) 
COMMENT                             #334=(CLabel (CWidget 0 (0 0) 1 2 0 0 #250# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (829)" "@N (@S)" 5 9.11129 
COMMENT                                   11.978 0 -10 4.47082 1.07074 #333#) 
COMMENT                             #335=(CScratch 
COMMENT                                   (CWidget 3 (1 #2# 3) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.11516 1.9 0.082322 1) 
COMMENT                             #336=(CLabel (CWidget 0 (0 0) 1 2 0 0 #250# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (3342)" "@N (@S)" 5 14.5027 
COMMENT                                   -7.59515 0 -10 4.90287 1.07074 #335#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #337=(CGroupWidget 
COMMENT                            (CWidget 6 (10 #0# 0) 1 2 0 0 Nil 1382 100) 
COMMENT                            (CObjectList 
COMMENT                             #338=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 6.12979 1.9 0.082322 1) 
COMMENT                             #339=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #250# 1413698627 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (223)" "@N (@S)" 5 5.71572 
COMMENT                                   18.5658 0 -10 5.16586 1.07074 #338#) 
COMMENT                             #340=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.96983 1.9 0.082322 1) 
COMMENT                             #341=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #250# 1195591508 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (3567)" "@N (@S)" 5 13.7684 
COMMENT                                   -11.8654 0 -10 5.59791 1.07074 #340#) 
COMMENT                             #342=(CScratch 
COMMENT                                   (CWidget 3 (1 #0# 3) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.7683 1.9 0.082322 1) 
COMMENT                             #343=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #250# 1413955924 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (3879)" "@N (@S)" 5 12.0806 
COMMENT                                   -15.0152 0 -10 5.59791 1.07074 #342#) 
COMMENT                             #344=(CScratch 
COMMENT                                   (CWidget 4 (1 #0# 4) 1 2 0 0 #251# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.40917 1.9 0.082322 1) 
COMMENT                             #345=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #250# 1413955924 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (4435)" "@N (@S)" 5 7.13796 
COMMENT                                   -17.4746 0 -10 5.59791 1.07074 #344#) 
COMMENT                             #346=(CScratch 
COMMENT                                   (CWidget 5 (1 #0# 5) 1 2 0 0 #251# 51177000 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 3.03195 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #347=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #250# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (5019)" "@N (@S)" 5 -4.50207 
COMMENT                                   -16.1204 0 -10 5.59791 1.07074 #346#) 
COMMENT                             #348=(CScratch 
COMMENT                                   (CWidget 6 (1 #0# 6) 1 2 0 0 #251# 1037 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 0.615563 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #349=(CLabel (CWidget 0 (0 0) 1 2 0 0 #250# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (8760)" "@N (@S)" 5 -11.0737 
COMMENT                                   12.1963 0 -10 5.59791 1.07074 #348#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #350=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1588 100) 
COMMENT                     (CObjectList 
COMMENT                      #351=(CLabel 
COMMENT                            (CWidget 0 (15 #30# 0) 1 2 0 0 #222# 1413955924 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 56 21 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 18-JUN-2018" 
COMMENT                            "Last modified: 18-JUN-2018" 65535 0.0399468 
COMMENT                            -4.39414 0 -10 11.3461 1.07074 Nil) 
COMMENT                      #352=(CLabel 
COMMENT                            (CWidget 0 (15 #31# 0) 1 2 0 0 #222# 1195590484 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 84 31 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 10386" 
COMMENT                            "LMBP 10386" 65535 0.359521 -2.74301 0 -10 7.53276 
COMMENT                            1.55915 Nil)) (CObjectList)) 
COMMENT               #353=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1167 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 63318152 
COMMENT       (CStringList) 63327792 63330272 (CObList)))
FEATURES             Location/Qualifiers
     CDS             7771..8478
                     /vntifkey="4"
                     /label=rtTA3
     RBS             8535..9107
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             9110..9712
                     /vntifkey="4"
                     /label=puromycin\resistance
     terminator      397..471
                     /vntifkey="43"
                     /label=SV40\polyA\early
     promoter        3653..3981
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      900..1355
                     /vntifkey="33"
                     /label=f1\origin
     CDS             1486..2346
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      2887..3289
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        3381..3464
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        3528..3544
                     /vntifkey="30"
                     /label=T3\promoter
     terminator      complement(397..471)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      607..682
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(716..732)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    4092..4136
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
     misc_feature    4646..4879
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
     terminator      91..325
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    75..90
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     promoter        5397..5664
                     /vntifkey="29"
                     /label=hCMVTRE\promoter
     promoter        7380..7662
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      7619..7694
                     /vntifkey="33"
                     /label=SV40\ori
     misc_feature    8479..8512
                     /vntifkey="21"
                     /label=loxP
     misc_marker     7321..7344
                     /vntifkey="22"
                     /label=FLAG\epitope
     attenuator      5707..5731
                     /vntifkey="0"
                     /label=attB1
     CDS             5779..7236
                     /vntifkey="4"
                     /label=mRipk3
     attenuator      complement(7253..7277)
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     2477 a      2451 c      2536 g      2248 t 
ORIGIN
        1 gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat 
       61 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 
      121 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 
      181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 
      241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 
      301 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 
      361 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 
      421 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
      481 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 
      541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
      601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
      661 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag 
      721 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
      781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
      841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 
      901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
      961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     1021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 
     1081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     1141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     1201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     1261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     1321 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 
     1381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     1441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 
     1501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 
     1561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 
     1621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 
     1681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 
     1741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 
     1801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 
     1861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 
     1921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 
     1981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 
     2041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 
     2101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 
     2161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 
     2221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 
     2281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 
     2341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 
     2401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     2461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     2521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     2581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     2641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 
     2701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     2761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     2821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     2881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     2941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     3001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     3061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     3121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     3181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     3241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 
     3301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     3361 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
     3421 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
     3481 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 
     3541 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt 
     3601 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg 
     3661 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg 
     3721 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt 
     3781 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct 
     3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc 
     4021 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg 
     4081 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg 
     4141 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt 
     4201 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct 
     4261 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact 
     4321 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac 
     4381 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt 
     4441 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga 
     4501 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 
     4561 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 
     4621 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 
     4681 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 
     4741 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 
     4801 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 
     4861 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 
     4921 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 
     4981 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 
     5041 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 
     5101 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 
     5161 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata 
     5221 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac 
     5281 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc 
     5341 gattagtgaa cggatctcga cggtatcgat ggtccgaggt tctagacgag tttactccct 
     5401 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga 
     5461 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 
     5521 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat 
     5581 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag 
     5641 ctcgtttagt gaaccgtcag atcgcaccgg tcactagtcc agtgtggtgg aattctgcag 
     5701 atatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga 
     5761 tccggtaccg aattcaccat gtcttctgtc aagttatggc ctactggtgc gtcagcggtt 
     5821 cctctggtga gccgtgaaga actgaagaag ctggagtttg tgggcaaagg agggttcgga 
     5881 gtcgtgttcc gggcacacca cagaacatgg aaccatgatg tagcagtcaa gatcgtgaac 
     5941 tcgaagaaga tatcctggga ggtgaaggct atggttaatc ttcgtaatga gaacgttctg 
     6001 ctcctgctgg gggtcactga ggacctccag tgggacttcg tgtccgggca ggctctggtg 
     6061 acaagattca tggagaatgg ctccctcgca gggctgctgc aacccgagtg ccctcggccc 
     6121 tggccactcc tctgtcgcct gctgcaggaa gtggtgctgg ggatgtgcta cctacacagc 
     6181 ttgaaccctc cgctcctgca ccgggacctc aagccctcta acattctgct ggatccagag 
     6241 ctccacgcca agctagcaga ttttggcctg tccacgtttc agggagggtc ccagtcaggg 
     6301 tcaggatcag gatcaggatc cagggactct gggggcaccc tagcgtactt ggacccagag 
     6361 ctgttatttg atgtcaacct gaaggcttct aaagcgagtg atgtctacag ctttgggatc 
     6421 ctcgtgtggg cagtgctggc tggcagagaa gctgagttgg tagacaagac ttcactaatc 
     6481 cgggaaacag tgtgtgacag gcagagtcgt cctccactga cagagctgcc tccaggtagc 
     6541 cctgagactc ctggcttgga aaaactgaag gagttaatga ttcattgctg gggttcccag 
     6601 tccgaaaaca ggccatcctt ccaggactgc gaaccaaaaa ccaatgaagt ttacaatctg 
     6661 gtaaaggaca aggtagatgc tgctgtctcc gaggtaaagc attatctgtc tcagcacaga 
     6721 agcagcggca gaaacttgtc tgccagagag ccaagccaaa gaggcacaga aatggattgc 
     6781 ccgagggaaa ccatggtttc taaaatgctg gaccgcctgc atttggagga accctccgga 
     6841 ccagttcctg gaaaatgtcc tgagaggcaa gcacaggaca catcagttgg gcctgccaca 
     6901 ccagcaagga catcttctga ccccgtggct ggcactcctc agattccaca tactttaccc 
     6961 ttcagaggca caacacctgg gccagtcttt actgagactc ccggtcctca cccccaaagg 
     7021 aatcagggag atggaagaca cggcactcct tggtatccct ggaccccacc gaatccaatg 
     7081 acagggccac cggctctcgt cttcaacaac tgttctgaag tgcagattgg gaactacaac 
     7141 tccttggtag caccaccaag aactactgcc tcaagttcgg ccaagtatga ccaagcacag 
     7201 ttcggcaggg gtaggggctg gcagcccttc cacaagcact cgagatatct agacccagct 
     7261 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg 
     7321 gactacaagg atgacgatga caaaacgcgt accggttagt aatgagtttg gaattaattc 
     7381 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt 
     7441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
     7501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
     7561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     7621 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 
     7681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 
     7741 tccattttcg gatctgatca gcacgtgacc atgtctaggc tggacaagag caaagtcata 
     7801 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc 
     7861 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc 
     7921 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg 
     7981 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc 
     8041 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac 
     8101 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca 
     8161 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag 
     8221 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg 
     8281 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg 
     8341 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac 
     8401 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ttttgacctt 
     8461 gacatgctcc ccgggtaaat aacttcgtat agtatacatt atacgaagtt atggatccgc 
     8521 ggccgcaaat tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 
     8581 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 
     8641 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 
     8701 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 
     8761 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 
     8821 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 
     8881 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 
     8941 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat 
     9001 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc 
     9061 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggccaccga 
     9121 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 
     9181 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 
     9241 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 
     9301 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 
     9361 agcgggggcg gtgttcgccg agatcggctc gcgcatggcc gagttgagcg gttcccggct 
     9421 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 
     9481 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 
     9541 cgtgctcccc ggagtggagg cggccgagcg cgctggggtg cccgccttcc tggagacctc 
     9601 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 
     9661 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct ga 
//
