ID pDG2i-mRipk3-Flag\puro standard; circular DNA; UNA; 9712 BP. DT 18-JUN-2018 DE LMBP 10386. CC Accession number: LMBP 10386 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 18-JUN-2018 CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|-12338795| CC VNTDBDATE|-12338795| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark 927| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid CC VNTEXTCHREPL|Bacteria CC VNTEXTCHREPL|Animal/Other Eukaryotic FH Key Location/Qualifiers FH FT CDS 7771..8478 FT /vntifkey="4" FT /label=rtTA3 FT RBS 8535..9107 FT /vntifkey="32" FT /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) FT CDS 9110..9712 FT /vntifkey="4" FT /label=puromycin\resistance FT terminator 397..471 FT /vntifkey="43" FT /label=SV40\polyA\early FT promoter 3653..3981 FT /vntifkey="29" FT /label=RSV/HIV-1\5'\LTR FT rep_origin 900..1355 FT /vntifkey="33" FT /label=f1\origin FT CDS 1486..2346 FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 2887..3289 FT /vntifkey="33" FT /label=pMB1\ori FT promoter 3381..3464 FT /vntifkey="30" FT /label=lac\promoter FT promoter 3528..3544 FT /vntifkey="30" FT /label=T3\promoter FT terminator complement(397..471) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin 607..682 FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(716..732) FT /vntifkey="30" FT /label=T7\promoter FT misc_feature 4092..4136 FT /vntifkey="21" FT /label=HIV-1\psi\packaging\signal FT misc_feature 4646..4879 FT /vntifkey="21" FT /label=HIV-1\Rev\response\element FT terminator 91..325 FT /vntifkey="43" FT /label=RSV/HIV-1\dU3/3'\LTR FT misc_feature 75..90 FT /vntifkey="21" FT /label=HIV-1\cPPT FT promoter 5397..5664 FT /vntifkey="29" FT /label=hCMVTRE\promoter FT promoter 7380..7662 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 7619..7694 FT /vntifkey="33" FT /label=SV40\ori FT misc_feature 8479..8512 FT /vntifkey="21" FT /label=loxP FT misc_marker 7321..7344 FT /vntifkey="22" FT /label=FLAG\epitope FT attenuator 5707..5731 FT /vntifkey="0" FT /label=attB1 FT CDS 5779..7236 FT /vntifkey="4" FT /label=mRipk3 FT attenuator complement(7253..7277) FT /vntifkey="0" FT /label=attB2 SQ Sequence 9712 BP; 2477 A; 2451 C; 2536 G; 2248 t; gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat 60 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 120 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 180 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 240 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 300 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 360 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 420 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 480 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 540 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 600 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 660 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag 720 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 780 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 840 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 900 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 960 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 1020 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 1080 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 1140 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 1200 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 1260 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 1320 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 1380 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 1440 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 1500 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 1560 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 1620 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 1680 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 1740 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 1800 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 1860 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 1920 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 1980 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 2040 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 2100 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 2160 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 2220 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 2280 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 2340 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 2400 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 2460 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 2520 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 2580 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 2640 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 2700 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 2760 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 2820 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 2880 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 2940 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 3000 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 3060 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 3120 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 3180 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 3240 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 3300 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 3360 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 3420 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 3480 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 3540 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt 3600 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg 3660 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg 3720 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt 3780 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct 3840 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 3900 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 3960 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc 4020 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg 4080 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg 4140 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt 4200 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct 4260 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact 4320 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac 4380 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt 4440 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga 4500 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata 4560 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc 4620 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag 4680 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg 4740 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc 4800 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc 4860 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg 4920 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga 4980 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg 5040 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg 5100 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa 5160 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata 5220 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac 5280 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc 5340 gattagtgaa cggatctcga cggtatcgat ggtccgaggt tctagacgag tttactccct 5400 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga 5460 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 5520 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat 5580 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag 5640 ctcgtttagt gaaccgtcag atcgcaccgg tcactagtcc agtgtggtgg aattctgcag 5700 atatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga 5760 tccggtaccg aattcaccat gtcttctgtc aagttatggc ctactggtgc gtcagcggtt 5820 cctctggtga gccgtgaaga actgaagaag ctggagtttg tgggcaaagg agggttcgga 5880 gtcgtgttcc gggcacacca cagaacatgg aaccatgatg tagcagtcaa gatcgtgaac 5940 tcgaagaaga tatcctggga ggtgaaggct atggttaatc ttcgtaatga gaacgttctg 6000 ctcctgctgg gggtcactga ggacctccag tgggacttcg tgtccgggca ggctctggtg 6060 acaagattca tggagaatgg ctccctcgca gggctgctgc aacccgagtg ccctcggccc 6120 tggccactcc tctgtcgcct gctgcaggaa gtggtgctgg ggatgtgcta cctacacagc 6180 ttgaaccctc cgctcctgca ccgggacctc aagccctcta acattctgct ggatccagag 6240 ctccacgcca agctagcaga ttttggcctg tccacgtttc agggagggtc ccagtcaggg 6300 tcaggatcag gatcaggatc cagggactct gggggcaccc tagcgtactt ggacccagag 6360 ctgttatttg atgtcaacct gaaggcttct aaagcgagtg atgtctacag ctttgggatc 6420 ctcgtgtggg cagtgctggc tggcagagaa gctgagttgg tagacaagac ttcactaatc 6480 cgggaaacag tgtgtgacag gcagagtcgt cctccactga cagagctgcc tccaggtagc 6540 cctgagactc ctggcttgga aaaactgaag gagttaatga ttcattgctg gggttcccag 6600 tccgaaaaca ggccatcctt ccaggactgc gaaccaaaaa ccaatgaagt ttacaatctg 6660 gtaaaggaca aggtagatgc tgctgtctcc gaggtaaagc attatctgtc tcagcacaga 6720 agcagcggca gaaacttgtc tgccagagag ccaagccaaa gaggcacaga aatggattgc 6780 ccgagggaaa ccatggtttc taaaatgctg gaccgcctgc atttggagga accctccgga 6840 ccagttcctg gaaaatgtcc tgagaggcaa gcacaggaca catcagttgg gcctgccaca 6900 ccagcaagga catcttctga ccccgtggct ggcactcctc agattccaca tactttaccc 6960 ttcagaggca caacacctgg gccagtcttt actgagactc ccggtcctca cccccaaagg 7020 aatcagggag atggaagaca cggcactcct tggtatccct ggaccccacc gaatccaatg 7080 acagggccac cggctctcgt cttcaacaac tgttctgaag tgcagattgg gaactacaac 7140 tccttggtag caccaccaag aactactgcc tcaagttcgg ccaagtatga ccaagcacag 7200 ttcggcaggg gtaggggctg gcagcccttc cacaagcact cgagatatct agacccagct 7260 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg 7320 gactacaagg atgacgatga caaaacgcgt accggttagt aatgagtttg gaattaattc 7380 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt 7440 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 7500 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 7560 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 7620 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 7680 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 7740 tccattttcg gatctgatca gcacgtgacc atgtctaggc tggacaagag caaagtcata 7800 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc 7860 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc 7920 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg 7980 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc 8040 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac 8100 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca 8160 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag 8220 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg 8280 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg 8340 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac 8400 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ttttgacctt 8460 gacatgctcc ccgggtaaat aacttcgtat agtatacatt atacgaagtt atggatccgc 8520 ggccgcaaat tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 8580 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 8640 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 8700 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 8760 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 8820 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 8880 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca 8940 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat 9000 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc 9060 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggccaccga 9120 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 9180 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 9240 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 9300 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 9360 agcgggggcg gtgttcgccg agatcggctc gcgcatggcc gagttgagcg gttcccggct 9420 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 9480 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 9540 cgtgctcccc ggagtggagg cggccgagcg cgctggggtg cccgccttcc tggagacctc 9600 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 9660 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct ga 9712 //