LOCUS       pFastBac1-hGABBR2-Avi-His        7495 bp ds-DNA     circular SYN 26-JAN-2016
DEFINITION  LMBP 10437.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     Transform into DH10Bac(TM) E. coli cells.
COMMENT     VNTDATE|694310400|
COMMENT     VNTDBDATE|759801600|
COMMENT     VNTNAME|pFastBac1-hGABBR2-Avi-His|
COMMENT     VNTAUTHORNAME|Invitrogen (Life Technologies)|
FEATURES             Location/Qualifiers
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4060..4065
                     /label=Kozak
     misc_feature    4066..6714
                     /label=GABABR2
     CDS             4066..6714
                     /codon_start=1
                     /label=Feature 16
     CDS             6724..6735
                     /codon_start=1
                     /label=Caspase 3 site
     CDS             6754..6798
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
     CDS             6811..6828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
     polyA_signal    6985..7119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  7148..7313
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattcg ccaccatggg cctcatgccg
     4081 ctcaccaagg aggtggccaa gggcagcatc gggcgcggtg tgctccccgc cgtggaactg
     4141 gccatcgagc agatccgcaa cgagtcactc ctgcgcccct acttcctcga cctgcggctc
     4201 tatgacacgg agtgcgacaa cgcaaaaggg ttgaaagcct tctacgatgc aataaaatac
     4261 gggccgaacc acttgatggt gtttggaggc gtctgtccat ccgtcacatc catcattgca
     4321 gagtccctcc aaggctggaa tctggtgcag ctttcttttg ctgcaaccac gcctgttcta
     4381 gccgataaga aaaaataccc ttatttcttt cggaccgtcc catcagacaa tgcggtgaat
     4441 ccagccattc tgaagttgct caagcactac cagtggaagc gcgtgggcac gctgacgcaa
     4501 gacgttcaga ggttctctga ggtgcggaat gacctgactg gagttctgta tggcgaggac
     4561 attgagattt cagacaccga gagcttctcc aacgatccct gtaccagtgt caaaaagctg
     4621 aaggggaatg atgtgcggat catccttggc cagtttgacc agaatatggc agcaaaagtg
     4681 ttctgttgtg catacgagga gaacatgtat ggtagtaaat atcagtggat cattccgggc
     4741 tggtacgagc cttcttggtg ggagcaggtg cacacggaag ccaactcatc ccgctgcctc
     4801 cggaagaatc tgcttgctgc catggagggc tacattggcg tggatttcga gcccctgagc
     4861 tccaagcaga tcaagaccat ctcaggaaag actccacagc agtatgagag agagtacaac
     4921 aacaagcggt caggcgtggg gcccagcaag ttccacgggt acgcctacga tggcatctgg
     4981 gtcatcgcca agacactgca gagggccatg gagacactgc atgccagcag ccggcaccag
     5041 cggatccagg acttcaacta cacggaccac acgctgggca ggatcatcct caatgccatg
     5101 aacgagacca acttcttcgg ggtcacgggt caagttgtat tccggaatgg ggagagaatg
     5161 gggaccatta aatttactca atttcaagac agcagggagg tgaaggtggg agagtacaac
     5221 gctgtggccg acacactgga gatcatcaat gacaccatca ggttccaagg atccgaacca
     5281 ccaaaagaca agaccatcat cctggagcag ctgcggaaga tctccctacc tctctacagc
     5341 atcctctctg ccctcaccat cctcgggatg atcatggcca gtgcttttct cttcttcaac
     5401 atcaagaacc ggaatcagaa gctcataaag atgtcgagtc catacatgaa caaccttatc
     5461 atccttggag ggatgctctc ctatgcttcc atatttctct ttggccttga tggatccttt
     5521 gtctctgaaa agacctttga aacactttgc accgtcagga cctggattct caccgtgggc
     5581 tacacgaccg cttttggggc catgtttgca aagacctgga gagtccacgc catcttcaaa
     5641 aatgtgaaaa tgaagaagaa gatcatcaag gaccagaaac tgcttgtgat cgtggggggc
     5701 atgctgctga tcgacctgtg tatcctgatc tgctggcagg ctgtggaccc cctgcgaagg
     5761 acagtggaga agtacagcat ggagccggac ccagcaggac gggatatctc catccgccct
     5821 ctcctggagc actgtgagaa cacccatatg accatctggc ttggcatcgt ctatgcctac
     5881 aagggacttc tcatgttgtt cggttgtttc ttagcttggg agacccgcaa cgtcagcatc
     5941 cccgcactca acgacagcaa gtacatcggg atgagtgtct acaacgtggg gatcatgtgc
     6001 atcatcgggg ccgctgtctc cttcctgacc cgggaccagc ccaatgtgca gttctgcatc
     6061 gtggctctgg tcatcatctt ctgcagcacc atcaccctct gcctggtatt cgtgccgaag
     6121 ctcatcaccc tgagaacaaa cccagatgca gcaacgcaga acaggcgatt ccagttcact
     6181 cagaatcaga agaaagaaga ttctaaaacg tccacctcgg tcaccagtgt gaaccaagcc
     6241 agcacatccc gcctggaggg cctacagtca gaaaaccatc acctgcgaat gaagatcaca
     6301 gagctggata aagacttgga agaggtcacc atgcagctgc aggacacacc agaaaagacc
     6361 acctacatta aacagaacca ctaccaagag ctcaatgaca tcctcaacct gggaaacttc
     6421 actgagagca cagatggagg aaaggccatt ttaaaaaatc acctcgatca aaatccccag
     6481 ctacagtgga acacaacaga gccctctcga acatgcaaag atcctataga agatataaac
     6541 tctccagaac acatccagcg tcggctgtcc ctccagctcc ccatcctcca ccacgcctac
     6601 ctcccatcca tcggaggcgt ggacgccagc tgtgtcagcc cctgcgtcag ccccaccgcc
     6661 agcccccgcc acagacatgt gccaccctcc ttccgagtca tggtctcggg cctgggtacc
     6721 agcgatgagg tggatggagg ttccggtggt tccggtctga atgatatctt tgaagctcag
     6781 aagattgaat ggcatgaagg acgtaccaag caccaccatc accatcacta atgatcactc
     6841 gaggcatgcg gtaccaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     6901 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6961 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     7021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7081 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
     7141 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     7201 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     7261 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     7321 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     7381 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     7441 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgg
//

