LOCUS       pFastBac1-hGRM5-mRuby2-His        9031 bp ds-DNA     circular SYN 27-JAN-2016
DEFINITION  LMBP 10442.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     Transform into DH10Bac(TM) E. coli cells.
COMMENT     VNTDATE|694310400|
COMMENT     VNTDBDATE|759888000|
COMMENT     VNTNAME|pFastBac1-hGRM5-mRuby2-His|
FEATURES             Location/Qualifiers
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4060..4065
                     /label=Kozak
     misc_feature    4066..7605
                     /label=mGluR5
     CDS             4066..7605
                     /codon_start=1
                     /label=Feature 14
     CDS             7612..7635
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
     CDS             7639..8349
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
     CDS             8350..8367
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
     polyA_signal    8521..8655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8684..8849
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattcg ccaccatggt ccttctgttg
     4081 atcctgtcag tcttactttt gaaagaagat gtccgtggga gtgcacagtc cagtgagagg
     4141 agggtggtgg ctcacatgcc gggtgacatc attattggag ctctcttttc tgttcatcac
     4201 cagcctactg tggacaaagt tcatgagagg aagtgtgggg cggtccgtga acagtatggc
     4261 attcagagag tggaggccat gctgcatacc ctggaaagga tcaattcaga ccccacactc
     4321 ttgcccaaca tcacactggg ctgtgagata agggactcct gctggcattc ggctgtggcc
     4381 ctagagcaga gcattgagtt cataagagat tccctcattt cttcagaaga ggaagaaggc
     4441 ttggtacgct gtgtggatgg ctcctcctct tccttccgct ccaagaagcc catagtaggg
     4501 gtcattgggc ctggctccag ttctgtagcc attcaggtcc agaatttgct ccagcttttc
     4561 aacatacctc agattgctta ctcagcaacc agcatggatc tgagtgacaa gactctgttc
     4621 aaatatttca tgagggttgt gccttcagat gctcagcagg caagggccat ggtggacata
     4681 gtgaagaggt acaactggac ctatgtatca gccgtgcaca cagaaggcaa ctatggagaa
     4741 agtgggatgg aagccttcaa agatatgtca gcgaaggaag ggatttgcat cgcccactct
     4801 tacaaaatct acagtaatgc aggggagcag agctttgata agctgctgaa gaagctcaca
     4861 agtcacttgc ccaaggcccg ggtggtggcc tgcttctgtg agggcatgac ggtgagaggt
     4921 ctgctgatgg ccatgaggcg cctgggtcta gcgggagaat ttctgcttct gggcagtgat
     4981 ggctgggctg acaggtatga tgtgacagat ggatatcagc gagaagctgt tggtggcatc
     5041 acaatcaagc tccaatctcc cgatgtcaag tggtttgatg attattatct gaagctccgg
     5101 ccagaaacaa accaccgaaa cccttggttt caagaatttt ggcagcatcg ttttcagtgc
     5161 cgactggaag ggtttccaca ggagaacagc aaatacaaca agacttgcaa tagttctctg
     5221 actctgaaaa cacatcatgt tcaggattcc aaaatgggat ttgtgatcaa cgccatctat
     5281 tcgatggcct atgggctcca caacatgcag atgtccctct gcccaggcta tgcaggactc
     5341 tgtgatgcca tgaagccaat tgatggacgg aaacttttgg agtccctgat gaaaaccaat
     5401 tttactgggg tttctggaga tacgatccta ttcgatgaga atggagactc tccaggaagg
     5461 tatgaaataa tgaatttcaa ggaaatggga aaagattact ttgattatat caacgttgga
     5521 agttgggaca atggagaatt aaaaatggat gatgatgaag tatggtccaa gaaaagcaac
     5581 atcatcagat ctgtgtgcag tgaaccatgt gagaaaggcc agatcaaggt gatccgaaag
     5641 ggagaagtca gctgttgttg gacctgtaca ccttgtaagg agaatgagta tgtctttgat
     5701 gagtacacat gcaaggcatg ccaactgggg tcttggccca ctgatgatct cacaggttgt
     5761 gacttgatcc cagtacagta tcttcgatgg ggtgaccctg aacccattgc agctgtggtg
     5821 tttgcctgcc ttggcctcct ggccaccctg tttgttactg tagtcttcat catttaccgt
     5881 gatacaccag tagtcaagtc ctcaagcagg gaactctgct acattatcct tgctggcatc
     5941 tgcctgggct acttatgtac cttctgcctc attgcgaagc ccaaacagat ttactgctac
     6001 cttcagagaa ttggcattgg tctctcccca gccatgagct actcagccct tgtaacaaag
     6061 accaaccgta ttgcaaggat cctggctggc agcaagaaga agatctgtac caaaaagccc
     6121 agattcatga gtgcctgtgc ccagctagtg attgctttca ttctcatatg catccagttg
     6181 ggcatcatcg ttgccctctt tataatggag cctcctgaca taatgcatga ctacccaagc
     6241 attcgagaag tctacctgat ctgtaacacc accaacctag gagttgtcac tccacttgga
     6301 tacaatggat tgttgatttt gagctgcacc ttctatgcgt tcaagaccag aaatgttcca
     6361 gctaacttca acgaggccaa gtatatcgcc ttcacaatgt acacgacctg cattatatgg
     6421 ctagcttttg tgccaatcta ctttggcagc aactacaaaa tcatcaccat gtgtttctcg
     6481 gtcagcctca gtgccacagt ggccctaggc tgcatgtttg tgccgaaggt gtacatcatc
     6541 ctggccaaac cagagagaaa cgtgcgcagc gccttcacca catctaccgt ggtgcgcatg
     6601 catgtagggg atggcaagtc atcctccgca gccagcagat ccagcagcct agtcaacctg
     6661 tggaagagaa ggggctcctc tggggaaacc ttaagttcca atggaaaatc cgtcacgtgg
     6721 gcccagaatg agaagagcag ccgggggcag cacctgtggc agcgcctgtc catccacatc
     6781 aacaagaaag aaaaccccaa ccaaacggcc gtcatcaagc ccttccccaa gagcacggag
     6841 agccgtggcc tgggcgctgg cgctggcgca ggcgggagcg ctgggggcgt gggggccacg
     6901 ggcggtgcgg gctgcgcagg cgccggccca ggcgggcccg agtccccaga cgccggcccc
     6961 aaggcgctgt atgatgtggc cgaggctgag gagcacttcc cggcgcccgc gcggccgcgc
     7021 tcaccgtcgc ccatcagcac gctgagccac cgcgcgggct cggccagccg cacggacgac
     7081 gatgtgccgt cgctgcactc ggagcctgtg gcgcgcagca gctcctcgca gggctccctc
     7141 atggagcaga tcagcagtgt ggtcacccgc ttcacggcca acatcagcga gctcaactcc
     7201 atgatgctgt ccaccgcggc ccccagcccc ggcgtcggcg ccccgctctg ctcgtcctac
     7261 ctgatcccca aagagatcca gttgcccacg accatgacga cctttgccga aatccagcct
     7321 ctgccggcca tcgaagtcac gggaggcgcg cagcccgcgg caggggcgca ggcggctggg
     7381 gacgcggccc gggagagccc cgcggccggt cccgaggctg cggccgccaa gccagacctg
     7441 gaggagctgg tggctctcac cccgccgtcc cccttcagag actcggtgga ctcggggagc
     7501 acaaccccca actcgccagt gtccgagtcg gccctctgta tcccgtcgtc tcccaaatat
     7561 gacactctta tcataagaga ttacactcag agctcctcgt cgttgggtac cctggaggtg
     7621 ctgttccagg gccccggaat ggtgtctaag ggcgaagagc tgatcaagga aaatatgcgt
     7681 atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga
     7741 gaaggcaatc cgtacatggg aactcaaacc atgaggatca aagtcatcga gggaggaccc
     7801 ctgccatttg cctttgacat tcttgccacg tcgttcatgt atggcagccg tacttttatc
     7861 aagtacccga aaggcattcc tgatttcttt aaacagtcct ttcctgaggg ttttacttgg
     7921 gaaagagtta cgagatacga agatggtgga gtcgtcaccg tcatgcagga caccagcctt
     7981 gaggatggct gtctcgttta ccacgtccaa gtcagagggg taaactttcc ctccaatggt
     8041 cccgtgatgc agaagaagac caagggttgg gagcctaata cagagatgat gtatccagca
     8101 gatggtggtc tgaggggata cactcatatg gcactgaaag ttgatggtgg tggccatctg
     8161 tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc
     8221 ggtatccatg ccgttgatca ccgcctggaa aggttagagg aaagtgacaa tgaaatgttc
     8281 gtagtacaac gcgaacacgc agttgccaag ttcgccgggc ttggtggtgg gatggacgag
     8341 ctgtacaagc atcaccatca ccatcattaa tgactcgagg catgcggtac caagcttgtc
     8401 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     8461 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8521 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8581 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8641 tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat
     8701 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     8761 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     8821 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
     8881 atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc
     8941 acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc
     9001 aacgcttatt tgcagcctga atggcgaatg g
//

