LOCUS       pFastBac1-hGNAI1-Avi-His        5902 bp ds-DNA     circular SYN 24-MRT-2016
DEFINITION  LMBP 10444.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     Transform into DH10Bac(TM) E. coli cells.
COMMENT     VNTDATE|694310400|
COMMENT     VNTDBDATE|765158400|
COMMENT     VNTNAME|pFastBac1-hGNAI1-Avi-His|
FEATURES             Location/Qualifiers
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4060..5121
                     /codon_start=1
                     /label=GNAI1
     CDS             5131..5142
                     /codon_start=1
                     /label=Caspase 3 site
     CDS             5161..5205
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
     CDS             5218..5235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
     polyA_signal    5392..5526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  5555..5720
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattca tgggctgcac gctgagcgcc
     4081 gaggacaagg cggcggtgga gcggagtaag atgatcgacc gcaacctccg tgaggacggc
     4141 gagaaggcgg cgcgcgaggt caagctgctg ctgctcggtg ctggtgaatc tggtaaaagt
     4201 acaattgtga agcagatgaa aattatccat gaagctggtt attcagaaga ggagtgtaaa
     4261 caatacaaag cagtggtcta cagtaacacc atccagtcaa ttattgctat cattagggct
     4321 atggggaggt tgaagataga ctttggtgac tcagcccggg cggatgatgc acgccaactc
     4381 tttgtgctag ctggagctgc tgaagaaggc tttatgactg cagaacttgc tggagttata
     4441 aagagattgt ggaaagatag tggtgtacaa gcctgtttca acagatcccg agagtaccag
     4501 cttaatgatt ctgcagcata ctatttgaat gacttggaca gaatagctca accaaattac
     4561 atcccgactc aacaagatgt tctcagaact agagtgaaaa ctacaggaat tgttgaaacc
     4621 cattttactt tcaaagatct tcattttaaa atgtttgatg tgggaggtca gagatctgag
     4681 cggaagaagt ggattcattg cttcgaagga gtgacggcga tcatcttctg tgtagcactg
     4741 agtgactacg acctggttct agctgaagat gaagaaatga accgaatgca tgaaagcatg
     4801 aaattgtttg acagcatatg taacaacaag tggtttacag atacatccat tatacttttt
     4861 ctaaacaaga aggatctttt tgaagaaaaa atcaaaaaga gccctctcac tatatgctat
     4921 ccagaatatg caggatcaaa cacatatgaa gaggcagctg catatattca atgtcagttt
     4981 gaagacctca ataaaagaaa ggacacaaag gaaatataca cccacttcac atgtgccaca
     5041 gatactaaga atgtgcagtt tgtttttgat gctgtaacag atgtcatcat aaaaaataat
     5101 ctaaaagatt gtggtctctt tggtaccagc gatgaggtgg atggaggttc cggtggttcc
     5161 ggtctgaatg atatctttga agctcagaag attgaatggc atgaaggacg taccaagcac
     5221 caccatcacc atcactaatg atcactcgag gcatgcggta ccaagcttgt cgagaagtac
     5281 tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     5341 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     5401 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5461 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5521 tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca
     5581 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     5641 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     5701 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
     5761 cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga
     5821 aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat
     5881 ttgcagcctg aatggcgaat gg
//

