LOCUS       pOPINE-HA-hDRD2-BRIL-Avi-His        6462 bp ds-DNA     circular SYN 17-JUN-2017
DEFINITION  LMBP 10445.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|462844800|
COMMENT     VNTDBDATE|808790400|
COMMENT     VNTNAME|pOPINE-HA-hDRD2-BRIL-Avi-His|
COMMENT     VNTAUTHORNAME|nick berrow|
FEATURES             Location/Qualifiers
     misc_recomb     45..1011
                     /label=lef2/ORF 603
                     /note="lef2/ORF 603"
     enhancer        1079..1443
                     /label=CMV Enhancer
                     /note="CMV Enhancer"
     promoter        1449..1726
                     /label=Chicken -Actin Promoter
                     /note="Chicken  -Actin Promoter"
     promoter        2150..2166
                     /label=T7 Promoter
                     /note="T7 Promoter"
     protein_bind    2171..2191
                     /label=lac Operator
                     /note="lac Operator"
     promoter        2205..2318
                     /label=p10 Promoter+5'UTR
                     /note="p10 Promoter+5'UTR"
     CDS             2333..2380
                     /codon_start=1
                     /label=HA ss
     CDS             2381..3601
                     /codon_start=1
                     /label=D2 deltaICL3_BRIL
     CDS             3041..3358
                     /codon_start=1
                     /label=BRIL codon opt
     mat_peptide     3605..3646
                     /label=Biotin Acceptor Peptide
     misc_feature    3647..3667
                     /label=KHHHHHH tag
                     /note="KHHHHHH tag"
     polyA_signal    3725..3961
                     /label=Rabbit -Globin Poly A Site
                     /note="Rabbit  -Globin Poly A Site"
     terminator      3965..4012
                     /label=T7 Terminator
                     /note="T7 Terminator"
     misc_recomb     4025..4730
                     /label=ORF 1629
                     /note="ORF 1629"
     rep_origin      4941
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     CDS             complement(5543..6400)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaaaac
     2341 catcattgct ttgagctaca ttttctgtct ggctctcggc atggatccac tgaatctgtc
     2401 ctggtatgat gatgatctgg agaggcagaa ctggagccgg cccttcaacg ggtcagacgg
     2461 gaaggcggac agaccccact acaactacta tgccacactg ctcaccctgc tcatcgctgt
     2521 catcgtcttc ggcaacgtgc tggtgtgcat ggctgtgtcc cgcgagaagg cgctgcagac
     2581 caccaccaac tacctgatcg tcagcctcgc agtggccgac ctcctcgtcg ccacactggt
     2641 catgccctgg gttgtctacc tggaggtggt aggtgagtgg aaattcagca ggattcactg
     2701 tgacatcttc gtcactctgg acgtcatgat gtgcacggcg agcatcctga acttgtgtgc
     2761 catcagcatc gacaggtaca cagctgtggc catgcccatg ctgtacaata cgcgctacag
     2821 ctccaagcgc cgggtcaccg tcatgatctc catcgtctgg gtcctgtcct tcaccatctc
     2881 ctgcccactc ctcttcggac tcaataacgc agaccagaac gagtgcatca ttgccaaccc
     2941 ggccttcgtg gtctactcct ccatcgtctc cttctacgtg cccttcattg tcaccctgct
     3001 ggtctacatc aagatctaca ttgtcctccg cagacgccgc gctgatctcg aggataattg
     3061 ggaaaccctt aatgacaatc ttaaagtgat agaaaaggca gacaatgctg cacaagtaaa
     3121 ggatgcgctt acgaagatgc gtgcagcagc cctggacgcg cagaaagcta cacccccgaa
     3181 actggaagat aaaagccccg acagccccga aatgaaggac tttagacatg gtttcgatat
     3241 attggtcggt caaattgacg atgccctcaa attggccaat gaaggtaagg tgaaagaagc
     3301 tcaagcggct gcggaacaac tgaagacaac gaggaacgca tacatccaaa aatatcttct
     3361 ctcccagcag aaggagaaga aagccactca gatgctcgcc attgttctcg gcgtgttcat
     3421 catctgctgg ctgcccttct tcatcacaca catcctgaac atacactgtg actgcaacat
     3481 cccgcctgtc ctgtacagcg ccttcacgtg gctgggctat gtcaacagcg ccgtgaaccc
     3541 catcatctac accaccttca acattgagtt ccgcaaggcc ttcctgaaga tcctccactg
     3601 cggcctgaac gacatcttcg aggctcagaa aatcgaatgg cacgaaaaac atcaccatca
     3661 ccatcactaa gtgattaacc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     3721 gccaatgccc tggctcacaa ataccactga gatcgatctt tttccctctg ccaaaaatta
     3781 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     3841 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     3901 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     3961 gtaactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagc
     4021 atgcggagga aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag
     4081 acgcacctct gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt
     4141 attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata
     4201 attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag
     4261 cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat
     4321 tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc
     4381 tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt
     4441 gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc
     4501 tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat agagcttgga
     4561 catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct
     4621 cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg
     4681 tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt gcgatcgcat
     4741 aacttcgtat agcatacatt atacgaagtt ataagctcgg aacgctgcgc tcggtcgttc
     4801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4921 aggccgcgtt gctggcgttt ttccataggc tccgccccct gacgagcatc acaaaaatcg
     4981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5101 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     5161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5341 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5521 atctcaagaa gatcctttgt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6421 attgaagcat ttatcagggt tattgtctca tgtccgcgcg tt
//

