LOCUS       pOPINE-ME-BRIL-hDRD2-Avi-His        6846 bp ds-DNA     circular SYN 17-JUN-2017
DEFINITION  LMBP 10446.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|462844800|
COMMENT     VNTDBDATE|808790400|
COMMENT     VNTNAME|pOPINE-ME-BRIL-hDRD2-Avi-His|
COMMENT     VNTAUTHORNAME|nick berrow|
FEATURES             Location/Qualifiers
     misc_recomb     45..1011
                     /label=lef2/ORF 603
                     /note="lef2/ORF 603"
     enhancer        1079..1443
                     /label=CMV Enhancer
                     /note="CMV Enhancer"
     promoter        1449..1726
                     /label=Chicken -Actin Promoter
                     /note="Chicken  -Actin Promoter"
     promoter        2150..2166
                     /label=T7 Promoter
                     /note="T7 Promoter"
     protein_bind    2171..2191
                     /label=lac Operator
                     /note="lac Operator"
     promoter        2205..2318
                     /label=p10 Promoter+5'UTR
                     /note="p10 Promoter+5'UTR"
     CDS             2333..2395
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
     CDS             2399..2716
                     /codon_start=1
                     /label=BRIL codon opt
     CDS             2717..3985
                     /codon_start=1
                     /label=D2
     mat_peptide     3989..4030
                     /label=Biotin Acceptor Peptide
     misc_feature    4031..4051
                     /label=KHHHHHH tag
                     /note="KHHHHHH tag"
     polyA_signal    4109..4345
                     /label=Rabbit -Globin Poly A Site
                     /note="Rabbit  -Globin Poly A Site"
     terminator      4349..4396
                     /label=T7 Terminator
                     /note="T7 Terminator"
     misc_recomb     4409..5114
                     /label=ORF 1629
                     /note="ORF 1629"
     rep_origin      5325
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     CDS             complement(5927..6784)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaaatt
     2341 cttagtcaac gttgcccttg tttttatggt cgtgtacatt tcttacatct atgcgatggc
     2401 tgatctcgag gataattggg aaacccttaa tgacaatctt aaagtgatag aaaaggcaga
     2461 caatgctgca caagtaaagg atgcgcttac gaagatgcgt gcagcagccc tggacgcgca
     2521 gaaagctaca cccccgaaac tggaagataa aagccccgac agccccgaaa tgaaggactt
     2581 tagacatggt ttcgatatat tggtcggtca aattgacgat gccctcaaat tggccaatga
     2641 aggtaaggtg aaagaagctc aagcggctgc ggaacaactg aagacaacga ggaacgcata
     2701 catccaaaaa tatcttccct tcaacgggtc agacgggaag gcggacagac cccactacaa
     2761 ctactatgcc acactgctca ccctgctcat cgctgtcatc gtcttcggca acgtgctggt
     2821 gtgcatggct gtgtcccgcg agaaggcgct gcagaccacc accaactacc tgatcgtcag
     2881 cctcgcagtg gccgacctcc tcgtcgccac actggtcatg ccctgggttg tctacctgga
     2941 ggtggtaggt gagtggaaat tcagcaggat tcactgtgac atcttcgtca ctctggacgt
     3001 catgatgtgc acggcgagca tcctgaactt gtgtgccatc agcatcgaca ggtacacagc
     3061 tgtggccatg cccatgctgt acaatacgcg ctacagctcc aagcgccggg tcaccgtcat
     3121 gatctccatc gtctgggtcc tgtccttcac catctcctgc ccactcctct tcggactcaa
     3181 taacgcagac cagaacgagt gcatcattgc caacccggcc ttcgtggtct actcctccat
     3241 cgtctccttc tacgtgccct tcattgtcac cctgctggtc tacatcaaga tctacattgt
     3301 cctccgcaga cgccgcaagc gagtcaacac caaacgcagc agccgagctt tcagggccca
     3361 cctgagggct ccactaaagg gcaactgtac tcaccccgag gacatgaaac tctgcaccgt
     3421 tatcatgaag tctaatggga gtttcccagt gaacaggcgg agagtggagg ctgcccggcg
     3481 agcccaggag ctggagatgg agatgctctc cagcaccagc ccacccgaga ggacccggta
     3541 cagccccatc ccacccagcc accaccagct gactctcccc gacccgtccc accatggtct
     3601 ccacagcact cccgacagcc ccgccaaacc agagaagaat gggcatgcca aagaccaccc
     3661 caagattgcc aagatctttg agatccagac catgcccaat ggcaaaaccc ggacctccct
     3721 caagaccatg agccgtagga agctctccca gcagaaggag aagaaagcca ctcagatgct
     3781 cgccattgtt ctcggcgtgt tcatcatctg ctggctgccc ttcttcatca cacacatcct
     3841 gaacatacac tgtgactgca acatcccgcc tgtcctgtac agcgccttca cgtggctggg
     3901 ctatgtcaac agcgccgtga accccatcat ctacaccacc ttcaacattg agttccgcaa
     3961 ggccttcctg aagatcctcc actgcggcct gaacgacatc ttcgaggctc agaaaatcga
     4021 atggcacgaa aaacatcacc atcaccatca ctaagtgatt aacctcaggt gcaggctgcc
     4081 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatcga
     4141 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     4201 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     4261 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     4321 gagtttggca acatatgccc atatgtaact agcataaccc cttggggcct ctaaacgggt
     4381 cttgaggggt tttttgctga aagcatgcgg aggaaattct ccttgaagtt tccctggtgt
     4441 tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca
     4501 cgatacattg ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag
     4561 acaattgttg tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta
     4621 gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa
     4681 tagatttgta aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg
     4741 ctggactatc taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca
     4801 atctagcttt gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca
     4861 aaatattatg cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg
     4921 taaacacgtt aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc
     4981 gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca
     5041 tagccacacg acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg
     5101 agctttttgg aattgcgatc gcataacttc gtatagcata cattatacga agttataagc
     5161 tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     5521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgttaccaa tgcttaatca
     5941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6061 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6541 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgtccg
     6841 cgcgtt
//

