LOCUS       pOPINE-ME-hDRD2-EGFP-His        8796 bp ds-DNA     circular SYN 17-JUN-2017
DEFINITION  LMBP 10447.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|657504000|
COMMENT     VNTDBDATE|808790400|
COMMENT     VNTNAME|pOPINE-ME-hDRD2-EGFP-His|
COMMENT     VNTAUTHORNAME|Ray Owens|
FEATURES             Location/Qualifiers
     CDS             45..1011
                     /codon_start=1
                     /label=lef-2,603
     enhancer        1079..1443
                     /label=CMV ie enhancer
     promoter        1449..1726
                     /label=Chicken actin promoter
     promoter        2150..2166
                     /label=T7 promoter
     CDS             2171..2191
                     /codon_start=1
                     /label=lac operator
     promoter        2205..2318
                     /label=p10 promoter
     CDS             2333..2395
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
     CDS             2396..3724
                     /codon_start=1
                     /label=D2
     CDS             3728..4464
                     /codon_start=1
                     /label=3C-GFP
     misc_feature    4469..4492
                     /label=His8
     protein_bind    4573..4608
                     /label=HSV tag
     enhancer        4700..5199
                     /label=CITE
     CDS             5235..6035
                     /codon_start=1
                     /label=neo
     terminator      6036..6300
                     /label=rabbit globin terminator
     terminator      6299..6346
                     /label=T7 terminator
     CDS             6360..7065
                     /codon_start=1
                     /label=1629
     rep_origin      7275
                     /label=origin
     CDS             complement(7874..8734)
                     /codon_start=1
                     /label=bla (amp) sequence
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaaatt
     2341 cttagtcaac gttgcccttg tttttatggt cgtgtacatt tcttacatct atgcgatgga
     2401 tccactgaat ctgtcctggt atgatgatga tctggagagg cagaactgga gccggccctt
     2461 caacgggtca gacgggaagg cggacagacc ccactacaac tactatgcca cactgctcac
     2521 cctgctcatc gctgtcatcg tcttcggcaa cgtgctggtg tgcatggctg tgtcccgcga
     2581 gaaggcgctg cagaccacca ccaactacct gatcgtcagc ctcgcagtgg ccgacctcct
     2641 cgtcgccaca ctggtcatgc cctgggttgt ctacctggag gtggtaggtg agtggaaatt
     2701 cagcaggatt cactgtgaca tcttcgtcac tctggacgtc atgatgtgca cggcgagcat
     2761 cctgaacttg tgtgccatca gcatcgacag gtacacagct gtggccatgc ccatgctgta
     2821 caatacgcgc tacagctcca agcgccgggt caccgtcatg atctccatcg tctgggtcct
     2881 gtccttcacc atctcctgcc cactcctctt cggactcaat aacgcagacc agaacgagtg
     2941 catcattgcc aacccggcct tcgtggtcta ctcctccatc gtctccttct acgtgccctt
     3001 cattgtcacc ctgctggtct acatcaagat ctacattgtc ctccgcagac gccgcaagcg
     3061 agtcaacacc aaacgcagca gccgagcttt cagggcccac ctgagggctc cactaaaggg
     3121 caactgtact caccccgagg acatgaaact ctgcaccgtt atcatgaagt ctaatgggag
     3181 tttcccagtg aacaggcgga gagtggaggc tgcccggcga gcccaggagc tggagatgga
     3241 gatgctctcc agcaccagcc cacccgagag gacccggtac agccccatcc cacccagcca
     3301 ccaccagctg actctccccg acccgtccca ccatggtctc cacagcactc ccgacagccc
     3361 cgccaaacca gagaagaatg ggcatgccaa agaccacccc aagattgcca agatctttga
     3421 gatccagacc atgcccaatg gcaaaacccg gacctccctc aagaccatga gccgtaggaa
     3481 gctctcccag cagaaggaga agaaagccac tcagatgctc gccattgttc tcggcgtgtt
     3541 catcatctgc tggctgccct tcttcatcac acacatcctg aacatacact gtgactgcaa
     3601 catcccgcct gtcctgtaca gcgccttcac gtggctgggc tatgtcaaca gcgccgtgaa
     3661 ccccatcatc tacaccacct tcaacattga gttccgcaag gccttcctga agatcctcca
     3721 ctgcaaactg gaagttctgt ttcagggccc ggtgagcaag ggcgaggagc tgttcaccgg
     3781 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3841 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3901 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
     3961 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4021 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4081 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4141 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
     4201 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
     4261 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4321 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     4381 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4441 tctcggcatg gacgagctgt acaagaaaca tcaccatcac catcaccatc actgaattct
     4501 cagatctcgg cgcgcctgca ggtcgacggt accggttcga agcttgcggc cgcacagctg
     4561 tatacacgtg caagccagcc agaactcgcc ccggaagacc ccgaggatct cgagcaccac
     4621 catcaccatc accatcacta agtgattaac ctcaggtgca ggctgcctat cagaaggtgg
     4681 tggctggtgt ggtaattccg gttattttcc accatattgc cgtcttttgg caatgtgagg
     4741 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4801 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4861 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4921 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4981 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctcacctc aagcgtattc
     5041 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5101 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5161 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccgcgataa
     5221 ttcctgcagc caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg
     5281 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5341 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5401 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5461 cgggcgttcc ttgcgcagca gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5521 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5581 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5641 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5701 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5761 ggctcaaggc gcgtatgccc gacggcgatg atctcgtcgt gactcatggc gatgcctgct
     5821 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5881 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5941 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6001 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatcaattc tctagccaat
     6061 gccctggctc acaaatacca ctgagatcga tctttttccc tctgccaaaa attatgggga
     6121 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     6181 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     6241 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact
     6301 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg
     6361 aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac
     6421 ctctgttcac tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag
     6481 cgctagattc tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat
     6541 taaatttata atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt
     6601 tatatctgaa tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt
     6661 caaacaaggg ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg
     6721 ccacaaaact tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt
     6781 tgttttgtaa taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat
     6841 cactgtcgtt agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt
     6901 taacatcggg cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt
     6961 tagaagttgc ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg
     7021 ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc
     7081 gtatagcata cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc
     7141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7261 cgttgctggc gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc
     7321 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7381 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7441 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     7501 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7561 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7621 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7681 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7741 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7801 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7861 agaagatcct ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7921 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7981 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8041 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8101 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8161 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8221 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8281 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8341 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8401 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8461 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8521 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8581 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8641 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8701 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8761 gcatttatca gggttattgt ctcatgtccg cgcgtt
//

