LOCUS       pOPINE-ME-BRIL-hDRD2-EGFP-His        9057 bp ds-DNA     circular SYN 17-JUN-2017
DEFINITION  LMBP 10448.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|657504000|
COMMENT     VNTDBDATE|808790400|
COMMENT     VNTNAME|pOPINE-ME-BRIL-hDRD2-EGFP-His|
COMMENT     VNTAUTHORNAME|Ray Owens|
FEATURES             Location/Qualifiers
     CDS             45..1011
                     /codon_start=1
                     /label=lef-2,603
     enhancer        1079..1443
                     /label=CMV ie enhancer
     promoter        1449..1726
                     /label=Chicken actin promoter
     promoter        2150..2166
                     /label=T7 promoter
     CDS             2171..2191
                     /codon_start=1
                     /label=lac operator
     promoter        2205..2318
                     /label=p10 promoter
     CDS             2333..2395
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
     CDS             2399..2716
                     /codon_start=1
                     /label=BRIL codon opt
     CDS             2717..3985
                     /codon_start=1
                     /label=D2
     CDS             3989..4725
                     /codon_start=1
                     /label=3C-GFP
     misc_feature    4730..4753
                     /label=His8
     protein_bind    4834..4869
                     /label=HSV tag
     enhancer        4961..5460
                     /label=CITE
     CDS             5496..6296
                     /codon_start=1
                     /label=neo
     terminator      6297..6561
                     /label=rabbit globin terminator
     terminator      6560..6607
                     /label=T7 terminator
     CDS             6621..7326
                     /codon_start=1
                     /label=1629
     rep_origin      7536
                     /label=origin
     CDS             complement(8135..8995)
                     /codon_start=1
                     /label=bla (amp) sequence
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaaatt
     2341 cttagtcaac gttgcccttg tttttatggt cgtgtacatt tcttacatct atgcgatggc
     2401 tgatctcgag gataattggg aaacccttaa tgacaatctt aaagtgatag aaaaggcaga
     2461 caatgctgca caagtaaagg atgcgcttac gaagatgcgt gcagcagccc tggacgcgca
     2521 gaaagctaca cccccgaaac tggaagataa aagccccgac agccccgaaa tgaaggactt
     2581 tagacatggt ttcgatatat tggtcggtca aattgacgat gccctcaaat tggccaatga
     2641 aggtaaggtg aaagaagctc aagcggctgc ggaacaactg aagacaacga ggaacgcata
     2701 catccaaaaa tatcttccct tcaacgggtc agacgggaag gcggacagac cccactacaa
     2761 ctactatgcc acactgctca ccctgctcat cgctgtcatc gtcttcggca acgtgctggt
     2821 gtgcatggct gtgtcccgcg agaaggcgct gcagaccacc accaactacc tgatcgtcag
     2881 cctcgcagtg gccgacctcc tcgtcgccac actggtcatg ccctgggttg tctacctgga
     2941 ggtggtaggt gagtggaaat tcagcaggat tcactgtgac atcttcgtca ctctggacgt
     3001 catgatgtgc acggcgagca tcctgaactt gtgtgccatc agcatcgaca ggtacacagc
     3061 tgtggccatg cccatgctgt acaatacgcg ctacagctcc aagcgccggg tcaccgtcat
     3121 gatctccatc gtctgggtcc tgtccttcac catctcctgc ccactcctct tcggactcaa
     3181 taacgcagac cagaacgagt gcatcattgc caacccggcc ttcgtggtct actcctccat
     3241 cgtctccttc tacgtgccct tcattgtcac cctgctggtc tacatcaaga tctacattgt
     3301 cctccgcaga cgccgcaagc gagtcaacac caaacgcagc agccgagctt tcagggccca
     3361 cctgagggct ccactaaagg gcaactgtac tcaccccgag gacatgaaac tctgcaccgt
     3421 tatcatgaag tctaatggga gtttcccagt gaacaggcgg agagtggagg ctgcccggcg
     3481 agcccaggag ctggagatgg agatgctctc cagcaccagc ccacccgaga ggacccggta
     3541 cagccccatc ccacccagcc accaccagct gactctcccc gacccgtccc accatggtct
     3601 ccacagcact cccgacagcc ccgccaaacc agagaagaat gggcatgcca aagaccaccc
     3661 caagattgcc aagatctttg agatccagac catgcccaat ggcaaaaccc ggacctccct
     3721 caagaccatg agccgtagga agctctccca gcagaaggag aagaaagcca ctcagatgct
     3781 cgccattgtt ctcggcgtgt tcatcatctg ctggctgccc ttcttcatca cacacatcct
     3841 gaacatacac tgtgactgca acatcccgcc tgtcctgtac agcgccttca cgtggctggg
     3901 ctatgtcaac agcgccgtga accccatcat ctacaccacc ttcaacattg agttccgcaa
     3961 ggccttcctg aagatcctcc actgcaaact ggaagttctg tttcagggcc cggtgagcaa
     4021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4081 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4141 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4201 cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     4261 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4321 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4381 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4441 caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt
     4501 gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4621 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaaac atcaccatca
     4741 ccatcaccat cactgaattc tcagatctcg gcgcgcctgc aggtcgacgg taccggttcg
     4801 aagcttgcgg ccgcacagct gtatacacgt gcaagccagc cagaactcgc cccggaagac
     4861 cccgaggatc tcgagcacca ccatcaccat caccatcact aagtgattaa cctcaggtgc
     4921 aggctgccta tcagaaggtg gtggctggtg tggtaattcc ggttattttc caccatattg
     4981 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5041 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5101 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5161 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5221 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5281 tggctcacct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5341 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5401 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     5461 gcttgccaca acccgcgata attcctgcag ccaatatggg atcggccatt gaacaagatg
     5521 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5581 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5641 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5701 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc agtgctcgac gttgtcactg
     5761 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5821 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5881 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5941 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6001 cgccagccga actgttcgcc aggctcaagg cgcgtatgcc cgacggcgat gatctcgtcg
     6061 tgactcatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6121 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6181 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6241 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6301 gggatcaatt ctctagccaa tgccctggct cacaaatacc actgagatcg atctttttcc
     6361 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     6421 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     6481 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     6541 aacatatgcc catatgtaac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     6601 ttttttgctg aaagcatgcg gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa
     6661 aggagtttgc accagacgca cctctgttca ctggtccggc gtattaaaac acgatacatt
     6721 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     6781 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     6841 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     6901 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     6961 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     7021 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     7081 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     7141 taaatagagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     7201 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     7261 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     7321 gaattgcgat cgcataactt cgtatagcat acattatacg aagttataag ctcggaacgc
     7381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga
     7561 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7621 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7681 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     7741 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7801 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7861 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7921 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7981 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8041 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8101 gcgcagaaaa aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac
     8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9001 tcctttttca atattattga agcatttatc agggttattg tctcatgtcc gcgcgtt
//

