LOCUS       pOPINE-ME-hDRD2-BRIL-EGFP-His        8688 bp ds-DNA     circular SYN 17-JUN-2017
DEFINITION  LMBP 10449.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|657504000|
COMMENT     VNTDBDATE|808790400|
COMMENT     VNTNAME|pOPINE-ME-hDRD2-BRIL-EGFP-His|
COMMENT     VNTAUTHORNAME|Ray Owens|
FEATURES             Location/Qualifiers
     CDS             45..1011
                     /codon_start=1
                     /label=lef-2,603
     enhancer        1079..1443
                     /label=CMV ie enhancer
     promoter        1449..1726
                     /label=Chicken actin promoter
     promoter        2150..2166
                     /label=T7 promoter
     CDS             2171..2191
                     /codon_start=1
                     /label=lac operator
     promoter        2205..2318
                     /label=p10 promoter
     CDS             2333..2395
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
     CDS             2396..3616
                     /codon_start=1
                     /label=D2 deltaICL3_BRIL
     CDS             3056..3373
                     /codon_start=1
                     /label=BRIL codon opt
     CDS             3620..4356
                     /codon_start=1
                     /label=3C-GFP
     misc_feature    4361..4384
                     /label=His8
     protein_bind    4465..4500
                     /label=HSV tag
     enhancer        4592..5091
                     /label=CITE
     CDS             5127..5927
                     /codon_start=1
                     /label=neo
     terminator      5928..6192
                     /label=rabbit globin terminator
     terminator      6191..6238
                     /label=T7 terminator
     CDS             6252..6957
                     /codon_start=1
                     /label=1629
     rep_origin      7167
                     /label=origin
     CDS             complement(7766..8626)
                     /codon_start=1
                     /label=bla (amp) sequence
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaaatt
     2341 cttagtcaac gttgcccttg tttttatggt cgtgtacatt tcttacatct atgcgatgga
     2401 tccactgaat ctgtcctggt atgatgatga tctggagagg cagaactgga gccggccctt
     2461 caacgggtca gacgggaagg cggacagacc ccactacaac tactatgcca cactgctcac
     2521 cctgctcatc gctgtcatcg tcttcggcaa cgtgctggtg tgcatggctg tgtcccgcga
     2581 gaaggcgctg cagaccacca ccaactacct gatcgtcagc ctcgcagtgg ccgacctcct
     2641 cgtcgccaca ctggtcatgc cctgggttgt ctacctggag gtggtaggtg agtggaaatt
     2701 cagcaggatt cactgtgaca tcttcgtcac tctggacgtc atgatgtgca cggcgagcat
     2761 cctgaacttg tgtgccatca gcatcgacag gtacacagct gtggccatgc ccatgctgta
     2821 caatacgcgc tacagctcca agcgccgggt caccgtcatg atctccatcg tctgggtcct
     2881 gtccttcacc atctcctgcc cactcctctt cggactcaat aacgcagacc agaacgagtg
     2941 catcattgcc aacccggcct tcgtggtcta ctcctccatc gtctccttct acgtgccctt
     3001 cattgtcacc ctgctggtct acatcaagat ctacattgtc ctccgcagac gccgcgctga
     3061 tctcgaggat aattgggaaa cccttaatga caatcttaaa gtgatagaaa aggcagacaa
     3121 tgctgcacaa gtaaaggatg cgcttacgaa gatgcgtgca gcagccctgg acgcgcagaa
     3181 agctacaccc ccgaaactgg aagataaaag ccccgacagc cccgaaatga aggactttag
     3241 acatggtttc gatatattgg tcggtcaaat tgacgatgcc ctcaaattgg ccaatgaagg
     3301 taaggtgaaa gaagctcaag cggctgcgga acaactgaag acaacgagga acgcatacat
     3361 ccaaaaatat cttctctccc agcagaagga gaagaaagcc actcagatgc tcgccattgt
     3421 tctcggcgtg ttcatcatct gctggctgcc cttcttcatc acacacatcc tgaacataca
     3481 ctgtgactgc aacatcccgc ctgtcctgta cagcgccttc acgtggctgg gctatgtcaa
     3541 cagcgccgtg aaccccatca tctacaccac cttcaacatt gagttccgca aggccttcct
     3601 gaagatcctc cactgcaaac tggaagttct gtttcagggc ccggtgagca agggcgagga
     3661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta
     3841 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3901 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3961 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4021 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4081 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4141 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4201 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4261 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4321 cgccgggatc actctcggca tggacgagct gtacaagaaa catcaccatc accatcacca
     4381 tcactgaatt ctcagatctc ggcgcgcctg caggtcgacg gtaccggttc gaagcttgcg
     4441 gccgcacagc tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat
     4501 ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg caggctgcct
     4561 atcagaaggt ggtggctggt gtggtaattc cggttatttt ccaccatatt gccgtctttt
     4621 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     4681 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4741 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4801 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4861 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctcacc
     4921 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4981 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     5041 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac
     5101 aacccgcgat aattcctgca gccaatatgg gatcggccat tgaacaagat ggattgcacg
     5161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5281 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5341 ggctggccac gacgggcgtt ccttgcgcag cagtgctcga cgttgtcact gaagcgggaa
     5401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5641 aactgttcgc caggctcaag gcgcgtatgc ccgacggcga tgatctcgtc gtgactcatg
     5701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat
     5941 tctctagcca atgccctggc tcacaaatac cactgagatc gatctttttc cctctgccaa
     6001 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     6061 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     6121 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     6181 ccatatgtaa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     6241 gaaagcatgc ggaggaaatt ctccttgaag tttccctggt gttcaaagta aaggagtttg
     6301 caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat tgttattagt
     6361 acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt
     6421 ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat
     6481 tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt
     6541 ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat
     6601 tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat
     6661 tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg
     6721 tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg ttaaatagag
     6781 cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc
     6841 aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat
     6901 taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt ggaattgcga
     6961 tcgcataact tcgtatagca tacattatac gaagttataa gctcggaacg ctgcgctcgg
     7021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7141 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg ccccctgacg agcatcacaa
     7201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     7381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7741 aaaaggatct caagaagatc ctttgttacc aatgcttaat cagtgaggca cctatctcag
     7801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8641 aatattattg aagcatttat cagggttatt gtctcatgtc cgcgcgtt
//

