LOCUS       pOPINE-ME-hCHRM1-Avi-His        6636 bp ds-DNA     circular SYN 18-JUN-2017
DEFINITION  LMBP 10450.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|462844800|
COMMENT     VNTDBDATE|808876800|
COMMENT     VNTNAME|pOPINE-ME-hCHRM1-Avi-His|
COMMENT     VNTAUTHORNAME|nick berrow|
FEATURES             Location/Qualifiers
     misc_recomb     45..1011
                     /label=lef2/ORF 603
                     /note="lef2/ORF 603"
     enhancer        1079..1443
                     /label=CMV Enhancer
                     /note="CMV Enhancer"
     promoter        1449..1726
                     /label=Chicken -Actin Promoter
                     /note="Chicken  -Actin Promoter"
     promoter        2150..2166
                     /label=T7 Promoter
                     /note="T7 Promoter"
     protein_bind    2171..2191
                     /label=lac Operator
                     /note="lac Operator"
     promoter        2205..2318
                     /label=p10 Promoter+5'UTR
                     /note="p10 Promoter+5'UTR"
     CDS             2333..2395
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
     CDS             2396..3775
                     /codon_start=1
                     /label=mAchR1
     mat_peptide     3779..3820
                     /label=Biotin Acceptor Peptide
     misc_feature    3821..3841
                     /label=KHHHHHH tag
                     /note="KHHHHHH tag"
     polyA_signal    3899..4135
                     /label=Rabbit -Globin Poly A Site
                     /note="Rabbit  -Globin Poly A Site"
     terminator      4139..4186
                     /label=T7 Terminator
                     /note="T7 Terminator"
     misc_recomb     4199..4904
                     /label=ORF 1629
                     /note="ORF 1629"
     rep_origin      5115
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     CDS             complement(5717..6574)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatgaaatt
     2341 cttagtcaac gttgcccttg tttttatggt cgtgtacatt tcttacatct atgcgatgaa
     2401 cacttcagcc ccacctgctg tcagccccaa catcaccgtc ctggcaccag gaaagggtcc
     2461 ctggcaagtg gccttcattg ggatcaccac gggcctcctg tcgctagcca cagtgacagg
     2521 caacctgctg gtactcatct ctttcaaggt caacacggag ctcaagacag tcaataacta
     2581 cttcctgctg agcctggcct gtgctgacct catcatcggt acattctcca tgaacctcta
     2641 taccacgtac ctgctcatgg gccactgggc tctgggcacg ctggcttgtg acctctggct
     2701 ggccctggac tatgtggcca gcaatgcctc cgtcatgaat ctgctgctca tcagctttga
     2761 ccgctacttc tccgtgactc ggcccctgag ctaccgtgcc aagcgcacac cccgccgggc
     2821 agctctgatg atcggcctgg cctggctggt ttcctttgtg ctctgggccc cagccatcct
     2881 cttctggcag tacctggtag gggagcggac agtgctagct gggcagtgct acatccagtt
     2941 cctctcccag cccatcatca cctttggcac agccatggct gccttctacc tccctgtcac
     3001 agtcatgtgc acgctctact ggcgcatcta ccgggagaca gagaaccgag cacgggagct
     3061 ggcagccctt cagggctccg agacgccagg caaagggggt ggcagcagca gcagctcaga
     3121 gaggtctcag ccaggggctg agggctcacc agagactcct ccaggccgct gctgtcgctg
     3181 ctgccgggcc cccaggctgc tgcaggccta cagctggaag gaagaagagg aagaggacga
     3241 aggctccatg gagtccctca catcctcaga gggagaggag cctggctccg aagtggtgat
     3301 caagatgcca atggtggacc ccgaggcaca ggcccccacc aagcagcccc cacggagctc
     3361 cccaaataca gtcaagaggc cgactaagaa agggcgtgat cgagctggca agggccagaa
     3421 gccccgtgga aaggagcagc tggccaagcg gaagaccttc tcgctggtca aggagaagaa
     3481 ggcggctcgg accctgagtg ccatcctcct ggccttcatc ctcacctgga caccgtacaa
     3541 catcatggtg ctggtgtcca ccttctgcaa ggactgtgtt cccgagaccc tgtgggagct
     3601 gggctactgg ctgtgctacg tcaacagcac catcaacccc atgtgctacg cactctgcaa
     3661 caaagccttc cgggacacct ttcgcctgct gctgctttgc cgctgggaca agagacgctg
     3721 gcgcaagatc cccaagcgcc ctggctccgt gcaccgcact ccctcccgcc aatgcggcct
     3781 gaacgacatc ttcgaggctc agaaaatcga atggcacgaa aaacatcacc atcaccatca
     3841 ctaagtgatt aacctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3901 gccctggctc acaaatacca ctgagatcga tctttttccc tctgccaaaa attatgggga
     3961 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     4021 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     4081 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact
     4141 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg
     4201 aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac
     4261 ctctgttcac tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag
     4321 cgctagattc tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat
     4381 taaatttata atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt
     4441 tatatctgaa tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt
     4501 caaacaaggg ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg
     4561 ccacaaaact tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt
     4621 tgttttgtaa taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat
     4681 cactgtcgtt agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt
     4741 taacatcggg cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt
     4801 tagaagttgc ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg
     4861 ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc
     4921 gtatagcata cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc
     4981 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5101 cgttgctggc gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc
     5161 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5221 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5281 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     5341 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5401 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5461 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5521 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5581 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5641 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5701 agaagatcct ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6601 gcatttatca gggttattgt ctcatgtccg cgcgtt
//

