LOCUS       mMKP-1                  5182 bp    DNA     circular SYN 28-JAN-2019
DEFINITION  LMBP 10463.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct

COMMENT     Accession number: LMBP 10463
            Last modified: 28-JAN-2019
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12661335|
COMMENT     VNTDBDATE|-12661342|
COMMENT     LSOWNER|
COMMENT     VNTNAME|mMKP-1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "mMKP-1" 0 5182 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 
COMMENT            (CStringList "/codon_start=1" "/gene=\"bla\"" 
COMMENT             "/product=\"beta-lactamase\"") 1 1 1 1 "1392..2252") 
COMMENT        #1=(CFSignal (CObList) "pMB1 ori" 33 0 0 2793 3195 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "SV40 polyA early" 43 0 1 3263 3337 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "SV40 polyA late" 43 0 0 3263 3337 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "T7 promoter" 30 0 1 4488 4504 0 (CStringList) 
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COMMENT        #5=(CFSignal (CObList) "f1 ori" 33 0 0 500 955 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 1 5 80 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 1 37 310 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "mDusp1" 4 0 1 3361 4338 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "rabbit \337-globin intron" 15 0 1 4559 5131 0 
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COMMENT                          "DNA Plasmid Phage Virus 'mMKP-1'" 1) 
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COMMENT                          "LMBP 10463." 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 01/28/19 12:30" 1) 
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COMMENT                          "Last Modified: 01/28/19 12:37" 1) 
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COMMENT                          "length: 5182 bp" 1) 
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COMMENT                          "Division: SYN" 1) 
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COMMENT                          "Modification Date in the Original DB: 28-JAN-2019" 1) 
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COMMENT                          "Sequence Source: synthetic DNA construct" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                          "Associated Genes:  bla" 1))) "Standard Fields") 
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COMMENT                          "REFERENCE   1  (bases 1 to 5182)" 1) 
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COMMENT                          "  AUTHORS   ." 1) 
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COMMENT                          "  TITLE     Direct Submission" 1) 
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COMMENT                          "  JOURNAL   Exported Jan 28, 2019 from SnapGene Viewer 4.2.3 to Vector NTI(R) " 
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COMMENT                          "            format" 1) 
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COMMENT                          "            http://www.snapgene.com" 1))) 
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FEATURES             Location/Qualifiers
     CDS             1392..2252
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(join(5162..5182,1..176))
                     /vntifkey="30"
                     /label=SV40\promoter
                     /note="SV40 early promoter"
     promoter        complement(join(5170..5182,1..198))
                     /vntifkey="30"
                     /label=SV40\promoter
                     /note="SV40 early promoter"
     rep_origin      join(5176..5182,1..129)
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 origin of replication"
     rep_origin      2793..3195
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3263..3337)
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      3263..3337
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(4488..4504)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      500..955
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(5..80)
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(37..310)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             complement(3361..4338)
                     /vntifkey="4"
                     /label=mDusp1
     intron          complement(4559..5131)
                     /vntifkey="15"
                     /label=rabbit\ß-globin\intron
BASE COUNT     1316 a      1304 c      1302 g      1260 t 
ORIGIN
        1 aaaaaagcct cttcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc 
       61 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag 
      121 gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct 
      181 ttgcatactt ctgcctgctg gggagcctgg ggactttcct tgctgactaa ttgagatgca 
      241 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca 
      301 cattccacag ctggttcttt ccgcctcaga agtcgacaat tcactggccg tcgttttaca 
      361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 
      421 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 
      481 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
      541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
      601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
      661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
      721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
      781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
      841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
      901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgattac aatttcctga 
      961 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 
     1021 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 
     1081 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 
     1141 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 
     1201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 
     1261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
     1321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
     1381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     1441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     1501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     1561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     1621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     1681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     1741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     1801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     1861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     1921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     1981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     2041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     2101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     2161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     2221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     2281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     2341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     2401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     2461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     2521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     2581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     2641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     2701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     2761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     2821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     2881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     2941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     3001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     3061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     3121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     3181 aggaagcgga agagtctaga gtcgaccaga catgataaga tacattgatg agtttggaca 
     3241 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 
     3301 tttatttgta accattataa gctgcaataa acaagttctg ctttaataag atctggatcc 
     3361 tcacaagtcc tcttcagaaa tgagcttttg ctcgctagct agcacttggg actcaaactg 
     3421 cagcagctgg cccatgaagc tgaagttcgg ggagatgata ctccgcctct gcttcacaaa 
     3481 ctcaaaggcc tcgtccagct ttacccggtt agtcctcatg aggtaagcaa ggcagatggt 
     3541 ggctgaccgg gagatgccgg cctggcaatg aacaaacact ctccctccag catccttgat 
     3601 ggagtctatg aagtcaatag cctcgttgaa ccaggagctg atgtctgcct tgtggttgtc 
     3661 ctccacaggg atgctcttgt actggtagtg accctcaaag tggttaggac aattggctga 
     3721 gacgttgatc aaggcggtga tgcccaaggc gtcaagcata tccttccgag aagcgtgata 
     3781 ggcactgccc aggtacagga aggacaggat ctccactggg cccccctgat cgtagagagg 
     3841 ggtactacag gagctgcatc cggattctgc actgtcaggc acactagtac tcagggggag 
     3901 gctgagcccc gtgggggtgg actgtttgct gcacagctca gggcaggaag ccgaaaacgc 
     3961 ttcatatcct ccttggagaa agaagacttg agtggagcgc gcctctcggc agagcgcgcc 
     4021 cgcggccagg gccagggtgc cgtcgcgctt ggcgccgtcc agggaggcgc tgcgctcgtc 
     4081 cagcagcacc acggcgtggt aggctccggc cagcaggcgg ccacgcagtt cagcgttggg 
     4141 cacgatatgc tccaggccca tggcgccctt ggcgcggcgc cgcacgatgg tgctgaagcg 
     4201 cacgttcact gagcccgcga tgtggccggc gttgaaagcg aagaaggagc gacaatccaa 
     4261 caacaggcac tgcgcggcgc cctctcgcag cagcgcgcgc agccccccgg cgtccaggat 
     4321 gcccacctcc atcaccatgg ccagccccag cgaccccagc gtgcccgttc tgcggttctc 
     4381 cagaaaaagt cggtcgatct tgtgcggtgt cctgtggccc taagtcccca agtgctcgct 
     4441 gctcctaatc tggcttcacc gcgctttcct cccgccggaa ttcgccctat agtgagtcgt 
     4501 attacaattc tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct 
     4561 gtaggaaaaa gaagaaggca tgaacatggt tagcagaggg gcccggtttg gactcagagt 
     4621 attttatcct catctcaaac agtgtatatc attgtaacca taaagagaaa ggcaggatga 
     4681 tgaccaggat gtagttgttt ctaccaataa gaatatttcc acgccagcca gaatttatat 
     4741 gcagaaatat tctaccttat catttaatta taacaattgt tctctaaaac tgtgctgaag 
     4801 tacaatataa tataccctga ttgccttgaa aaaaaagtga ttagagaaag tacttacaat 
     4861 ctgacaaata aacaaaagtg aatttaaaaa ttcgttacaa atgcaagcta aagtttaacg 
     4921 aaaaagttac agaaaatgaa aagaaaataa gaggagacaa tggttgtcaa cagagtagaa 
     4981 agtgaaagaa acaaaattat catgagggtc catggtgata caagggacat cttcccattc 
     5041 taaacaacac cctgaaaact ttgccccctc catataacat gaattttaca atagcgaaaa 
     5101 agaaagaaca atcaagggtc cccaaactca ccctgaagtt ctcaggatcg atccggagct 
     5161 ttttgcaaaa gcctaggcct cc 
//
