LOCUS       pNIM1T-rtTA            10031 bp    DNA     circular     19-JAN-2018
DEFINITION  LMBP 10511.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12122626|
COMMENT     VNTDBDATE|-12122626|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pNIM1T-rtTA|

COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pNIM1T-rtTA" 0 10031 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "SacI" "KpnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 654 659 1 10031 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 659 0 6874 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 7830 0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 30 0 0 627 643 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "627..643") 
COMMENT        #3=(CFSignal (CObList) "T3 promoter" 30 0 1 7845 7861 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6651..6667)") 
COMMENT        #4=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 21 0 1 7887 7924 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6693..6730)") 
COMMENT        #5=(CFSignal (CObList) "lac promoter" 30 0 1 7925 8008 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6731..6814)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 8100 8502 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6906..7308)") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 9043 9903 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(7849..8709)") 
COMMENT        #8=(CFSignal (CObList) "f1 ori" 33 0 1 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4..459)") 
COMMENT        #9=(CFSignal (CObList) "cartTA" 4 0 0 2291 3274 0 (CStringList) 
COMMENT            (CStringList "/gene=\"cartTA\"" "/codon_start=1" "/transl_table=12" 
COMMENT             "/product=\"reverse tetracycline regulated transactivator\"" 
COMMENT             "/protein_id=\"ART66054.1\"") 1 1 1 1 "1640..2623") 
COMMENT        #10=(CFSignal (CObList #11=(CLocSeg 0 562) #12=(CLocSeg 1217 9)) 
COMMENT             "CaSAT1" 4 0 1 3656 4882 0 (CStringList) 
COMMENT             (CStringList "/gene=\"CaSAT1\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"nourseothricin resistance marker\"" 
COMMENT              "/protein_id=\"ART66055.1\"") 1 1 1 1 
COMMENT             "complement(join(3005..3567,4222..4231))") 
COMMENT        #13=(CFSignal (CObList) "GFP" 4 0 1 5776 6492 0 (CStringList) 
COMMENT             (CStringList "/gene=\"GFP\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"green fluorescent protein\"" 
COMMENT              "/protein_id=\"ART66056.1\"") 1 1 1 1 "complement(5125..5841)") 
COMMENT        #14=(CFSignal (CObList) "5' CaADH1 integration site" 21 0 0 666 1393 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "15..742") 
COMMENT        #15=(CFSignal (CObList) "CaTDH3 promoter" 29 0 0 1400 2249 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "749..1598") 
COMMENT        #16=(CFSignal (CObList) "CaADH1" 21 0 0 2252 2290 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1601..1639") 
COMMENT        #17=(CFSignal (CObList) "CaQCR7 terminator" 43 0 0 3295 3649 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "2644..2998") 
COMMENT        #18=(CFSignal (CObList) "CaACT1 promoter" 29 0 1 4883 5380 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(4232..4729)") 
COMMENT        #19=(CFSignal (CObList) "CaACT1 terminator" 43 0 1 5387 5775 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "complement(4736..5124)") 
COMMENT        #20=(CFSignal (CObList) "CaOP4 promoter" 29 0 1 6503 6868 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(5852..6217)") 
COMMENT        #21=(CFSignal (CObList) "tet operator" 29 0 1 6878 7171 0 (CStringList) 
COMMENT             (CStringList "/regulatory_class=\"promoter\"") 1 1 1 1 
COMMENT             "complement(6227..6520)") 
COMMENT        #22=(CFSignal (CObList) "3' CaADH1 integration site" 21 0 0 7172 7818 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6521..7167")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #23=(CFSignal (CObList) "LMBP 10511" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
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COMMENT                   "" 0 4) 
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COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid Phage 'pNIM1T-rtTA'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP 10511." 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/19/18 10:01" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/19/18 10:01" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10031 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 18-JAN-2018" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  cartTA;  CaSAT1;  GFP" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #39=(CRefLinePar 
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COMMENT                   0 0) 
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COMMENT                                 "" 1))) "LMBP 10511"))) "Annotations") 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   1640..2623" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"cartTA\"" 1) 
COMMENT                                   #51=(CLinePar 
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COMMENT                                        "   /gene=\"CaSAT1\"" 1) 
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COMMENT                                        "   /codon_start=1" 1) 
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COMMENT                                        "   /transl_table=12" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /product=\"nourseothricin resistance marker\"" 
COMMENT                                        1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /protein_id=<a href=\"ncbi:protein/ART66055.1\">ART66055.1</a>" 
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COMMENT                                   #68=(CLinePar 
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COMMENT                                   #69=(CLinePar 
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COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"GFP\"" 1) 
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COMMENT                                        "   /codon_start=1" 1) 
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COMMENT                                        "   /protein_id=<a href=\"ncbi:protein/ART66056.1\">ART66056.1</a>" 
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COMMENT                                        "Start: 9043  End: 9903 (Complementary)" 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7849..8709)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (4 total)") 
COMMENT                     #79=(CFolderPar 
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COMMENT                                   #82=(CLinePar 
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COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   15..742" 1))) 
COMMENT                                 "5' CaADH1 integration site") 
COMMENT                            #84=(CFolderPar 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1601..1639" 1))) "CaADH1") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7172  End: 7818" 1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
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COMMENT                                        "   6521..7167" 1))) 
COMMENT                                 "3' CaADH1 integration site") 
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COMMENT                                 "5' UTR of lacZ, incl. RBS"))) 
COMMENT                          "Misc. Feature (4 total)") 
COMMENT                     #96=(CFolderPar 
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COMMENT                                        "Start: 1400  End: 2249" 1) 
COMMENT                                   #99=(CLinePar 
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COMMENT                                         "   /regulatory_class=\"promoter\"" 1))) 
COMMENT                                 "CaTDH3 promoter") 
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COMMENT                                    #108=(CLinePar 
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COMMENT                                  "CaACT1 promoter") 
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COMMENT                                  "CaOP4 promoter") 
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COMMENT                                    #120=(CLinePar 
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COMMENT                                          "   /regulatory_class=\"promoter\"" 1))) 
COMMENT                                  "tet operator"))) 
COMMENT                          "Promoter Eukaryotic (4 total)") 
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COMMENT                                           "   627..643" 1))) "T7 promoter") 
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COMMENT                                     #128=(CLinePar 
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COMMENT                                           "   complement(6651..6667)" 1))) 
COMMENT                                   "T3 promoter") 
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COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6731..6814)" 1))) 
COMMENT                                   "lac promoter"))) 
COMMENT                           "Promoter Prokaryotic (3 total)") 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #138=(CLinePar 
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COMMENT                                           "   complement(4..459)" 1))) "f1 ori") 
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COMMENT                                     #142=(CLinePar 
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COMMENT                                           "   complement(6906..7308)" 1))) 
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COMMENT                                           "Start: 3295  End: 3649" 1) 
COMMENT                                     #146=(CLinePar 
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COMMENT                                     #147=(CLinePar 
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COMMENT                                     #149=(CLinePar 
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COMMENT                                            " N1: 7830 " 1))))) "KpnI: 1 site") 
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COMMENT          #183=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
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FEATURES             Location/Qualifiers
     promoter        627..643
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(7845..7861)
                     /vntifkey="30"
                     /label=T3\promoter
     misc_feature    complement(7887..7924)
                     /vntifkey="21"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(7925..8008)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(8100..8502)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(9043..9903)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4..459)
                     /vntifkey="33"
                     /label=f1\ori
     misc_feature    666..1393
                     /vntifkey="21"
                     /label=5'\CaADH1\integration\site
     promoter        1400..2249
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaTDH3\promoter
     misc_feature    2252..2290
                     /vntifkey="21"
                     /label=CaADH1
     CDS             2291..3274
                     /gene="cartTA"
                     /codon_start=1
                     /transl_table=12
                     /product="reverse tetracycline regulated transactivator"
                     /protein_id="ART66054.1"
                     /vntifkey="4"
                     /label=cartTA
     terminator      3295..3649
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaQCR7\terminator
     CDS             complement(3656..4218,4873..4882)
                     /gene="CaSAT1"
                     /codon_start=1
                     /transl_table=12
                     /product="nourseothricin resistance marker"
                     /protein_id="ART66055.1"
                     /vntifkey="4"
                     /label=CaSAT1
     promoter        complement(4883..5380)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaACT1\promoter
     terminator      complement(5387..5775)
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaACT1\terminator
     CDS             complement(5776..6492)
                     /gene="GFP"
                     /codon_start=1
                     /transl_table=12
                     /product="green fluorescent protein"
                     /protein_id="ART66056.1"
                     /vntifkey="4"
                     /label=GFP
     promoter        complement(6503..6868)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaOP4\promoter
     promoter        complement(6878..7171)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=tet\operator
     misc_feature    7172..7818
                     /vntifkey="21"
                     /label=3'\CaADH1\integration\site
BASE COUNT     3066 a      1987 c      1998 g      2980 t 
ORIGIN
        1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
      601 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc 
      661 cgcggttgag atggagccgt caaatatcca aaacactctt ctttcaacta atttgccgta 
      721 aactatctcc atcgtacgat taagataaaa aaaaacaatt atttcttaca atatttgata 
      781 gagacccaat gcaaagccag cgagaaagag gagtattggc attgttgggt agtagatgaa 
      841 tgagtaatct tctagccaaa acaaaataaa ccacaagaac agtaggcaaa actgagtact 
      901 tgatagcgct tgtagtcttt gtatttaaaa aaacctaatt gtccaaaaat tgaaaatcat 
      961 gagttgcaaa tttgttgaaa tcaccaggta aatagatcat ttagctatat cgccttatca 
     1021 ttcttcgtac aagaattata cacaaatacc accaccacac cacccagaaa ttttacacgt 
     1081 gttttctaaa ttattacttt ccgtggccaa tcaaattgaa atttttcttg ttcgaaacgg 
     1141 cagtgcctca aacattatag tagcaagtat tgcctaaaga gtttccgtct cgtgcccccc 
     1201 ccctatcccc ttttgtaatt tgccaaaata gctcaaaaag gataatcccg cacttttttt 
     1261 ttccgagttc tcgtcatgca agcaagctta ttcagaattt tcagaggtgc gagtctcacc 
     1321 actaccactg cagctgcatc ttttacagca acagcaacag caggtgccac cacggcaaag 
     1381 acattgtctg gatctcgagc acacacatac agacaattgc gatattttat tttagggtca 
     1441 atttggaacg tcaccataca gtattcagta tgattaattt gacttgcaaa atacaaatcg 
     1501 cgtggttcaa ttccgaaaaa ttgatagtgt tccatgaaaa attacaagga gagccgtgaa 
     1561 ttataacaaa tttactcaca ttttggctgg caatttcttt aaccccctta atttttttca 
     1621 tgcagtttat tattttacgt agaagcttat tctctaaaca ctcggttatc cgaatatatt 
     1681 ttgctttcag agcaattgac tgatataata ccatcaattg aataaatcag tatcatcaaa 
     1741 tgctagtttg agttcaataa aaggtataat cggtgttaac tgaaaggtat tgtctaatta 
     1801 attaggttgc ggtttttgga tcagctgttt caaatccagg ctcgtggtgc acatttttta 
     1861 tttttgttgt gatgattttt tcttttcttt gcttaatagt tagttagtaa aatggataat 
     1921 gacgcaaaat tgcttctgaa tggatgataa tgccccgggt atggcgtgtc ccgtacaaat 
     1981 catattatgc aaaaatagcc aaaatagaca aaaataggtg gaaattgttt caaccaaccc 
     2041 actcaaccac taaaatttgt cttgtcaaag ccaattccaa cgaaaattgg attttttttg 
     2101 acttaatata tattaaccct tgaaattccc ttcaattgga aaattttttt aatttattta 
     2161 tttcttcttt ctttttttct ttttcttttt ctttctttta ttcatcacaa ttgaattcaa 
     2221 atcaattaac atcaacaaac tttacaatcg ttattgtcat acaacaacaa caacaaatac 
     2281 aaaaacaatt atgtctagat tagataaaag taaagtgatt aacggcgcat tagagttgct 
     2341 taatggggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga 
     2401 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttaccaat 
     2461 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga 
     2521 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc 
     2581 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt 
     2641 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgcagt 
     2701 ggggcatttt actttaggtt gcgtattgga agaacaagag catcaagtcg ctaaagaaga 
     2761 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt 
     2821 tgatagacaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt 
     2881 agaaaaacaa cttaaatgtg aaagtgggtc ctcgactttg tttggtggcg ccaattttaa 
     2941 tcaaagtggg aatattgctg atagctcatt gtccttcact ttcactaaca gtagcaacgg 
     3001 tccgaacctc ataacaactc aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc 
     3061 taacgttcat gataacttca tgaataatga aatcacggct agtaaaattg atgatggtaa 
     3121 taattcaaaa ccattgtcac ctggttggac ggaccaaact gcgtataacg cgtttggaat 
     3181 cactacaggg atgtttaata ccactacaat ggatgatgta tataactatc tattcgatga 
     3241 tgaagatacc ccaccaaacc caaaaaaaga gtaaggatct aaattctgga aatctaagta 
     3301 agtaagagga gtaggaggag gaagaagatg agaaggtcaa acattgaaat aggaaacaag 
     3361 aaagcaggag acaagtttaa agaatgaata ataaatattc ttatgaaagt tatatttatt 
     3421 tttgttagtt ttcttttaaa tatattcgta atgtcaatat aaaacaattt acaaaaagag 
     3481 attgtcaaaa agaagaaaca catattataa tctaagtcta ttaaatatct ctataaaaaa 
     3541 ataatgttat acttgaacaa aagcactatt ctttttgttt atctatatct atatatctat 
     3601 ctatcaaata tctttgtttt taatctgatc gactcattcc taatttaaac atatgttagg 
     3661 cgtcatcctg tgctcccgag aaccagtacc agtacatcgc tgtttcgttc gagacttgag 
     3721 gtctagtttt atacgtgaag aggtcaatgc cgccgagagt aaagccacat tttgcgtaca 
     3781 aattgcaggc aggtacattg ttcgtttgtg tctctaatcg tatgccaagg agctgtctgc 
     3841 ttagtgccca ctttttcgca aattcgatga gactgtgcgc gactcctttg cctcggtgcg 
     3901 tgtgcgacac aacaatgtgt tcgatagagg ctagatcgtt ccatgttgag ttgagttcaa 
     3961 tcttcccgac aagctcttgg tcgatgaatg cgccatagca agcagagtct tcatcagagt 
     4021 catcatccga gatgtaatcc ttccggtagg ggctcacact tctggtagat agttcaaagc 
     4081 cttggtcgga taggtgcaca tcgaacactt cacgaacaat gaaatggttc tcagcatcca 
     4141 atgtttccgc cacctgctca gggatcaccg aaattttcat atgagaaccg ttatcgataa 
     4201 ctaaagcagc aacttcttct ataaaaatgg gttagtatga cagtcattta aataaggaat 
     4261 ttttcagttg gcttggtttc aattcaatgt tcgttttttt tttttcttgc tgtgtttgtg 
     4321 tttgtgttgt ttatagttgt gtgcactgat cgtcgaaaaa aaaaattcat agtgagccgg 
     4381 gaaatctgta tagcccagat aacaacacaa gtccaaacta gaaactcgtc aaacaccaaa 
     4441 agcaatgttg aatcaattgc cttgcacaag tacacgtagg aaaacataaa acattgcaat 
     4501 tttgaatatt gagccttttg tcgtaacatt gattgatagg attactcacc gaatggtttt 
     4561 gaaaccactg ccgacagatc aatcaatcaa tcaaaaaacg tgaactttga aaaaggggaa 
     4621 gaacagatac attgaagtta gccatttcca ctgatcgtca caacatatct gataaattac 
     4681 tttcaaaatt ataagctgat gtgtgtgtat tattaatgtg acagtaacat cccaaacgag 
     4741 aaatattatc tcgacaacaa aaaagtttga tctgaattga aaatgaagtt ttcccaccct 
     4801 acccatttgt catattgaaa ccaatcaact gattaatcaa tcaattagaa ttgaagctaa 
     4861 actaaaacat accaccgtcc attttgaatg attatatttt tttaatatta atatcgagat 
     4921 aatgtttcta agaaagaaag aaaaccagga gtgaaaatta gaaaaggaaa ggaaaggaaa 
     4981 aaaagaaaaa tctgaaaata tataaaaaaa aattgtttcg ttggcaataa atcttggtga 
     5041 gaacagcgac cgaaagcaaa taagaacaaa atatgagtgt attacgttga acaactaatt 
     5101 aacgtgtgtg tatggatctt tttttctttt ttctctttaa ccgactataa acaacaaaca 
     5161 tttttgggca gtgcacacac tacttaatat acacagcata aattacacga ttagaaacaa 
     5221 attagcttat taaaataacc taatcaaacc gaatatttta tggtattatg agtaaactat 
     5281 ataatataaa tagcacacac ccacaacaac aacaaaggaa aactaaaagg ttttttcttt 
     5341 ttgaaaagat cgttttcttt attattctct agttttgacg ctcgacattt tatgatggaa 
     5401 tgaatgggat gaatcatcaa acaagagaaa atacccgtga cgaaaataat aaaataagtt 
     5461 cctctgatac agaagatgaa aacaacaaca acaagatata gaaatgcctt gggtggctat 
     5521 tttatagtct taacttttta atgtatattt gttttgtttt tttacataat aatactttat 
     5581 aaaagctaag ctaaattcaa gtaaaatttc aatctctcaa ataaaacatt tttctctttt 
     5641 tcttaaattt agttttatat atttataaaa tatacaaaga tttttttaaa aaagtaacaa 
     5701 gttatatatg taataacaaa aagaagaata acaagaatac aaaaccagat ttccagattt 
     5761 ccagaattta gatctttatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt 
     5821 tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga 
     5881 ttgtgtggac aagtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt 
     5941 ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa 
     6001 gttaactttg attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata 
     6061 gttgtattcc aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa 
     6121 ctcgattcta ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt 
     6181 cccgtcatct ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt 
     6241 gaaaaagtca tgccgtttca tatgatctgg gtatctcgca aagcattgaa gaccatacgc 
     6301 gaaagtagtg acaagtgttg gccatggaac aggtagtttt ccagtagtgc aaataaattt 
     6361 aagggtaagt tttccgtatg ttgcatcacc ttcaccctct ccactgacag aaaatttgtg 
     6421 cccattaaca tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc 
     6481 tcccttactc atctttgtcg actatttata tttgtatgtg tgtaggagtt aacgtagtag 
     6541 tagacgtagt tggttttatt gtagttgata ttgatttctt tctgagaagt tgaaagagtt 
     6601 tgttgatttg atttgataat gatataaaag aaataaaaaa aaatctaaaa cttgaataca 
     6661 agagatttga atctatattt atatttcaga aaagcccatc agaaacgaca aacatggaca 
     6721 acactttaat tgaaagggct ttatttttaa gtgaagcttt ttccttaaat cattgttagt 
     6781 aattcccata gttattgaat ctattactca atcgagtagt ttatatttaa tttatcgaca 
     6841 atattgcgaa aaataaaaaa gaagtttgga gctcgacttt cacttttctc tatcactgat 
     6901 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga 
     6961 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc 
     7021 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa 
     7081 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc 
     7141 tctatcactg atagggagtg gtaaactcga caaggtgctg aaccaaactg tggtgaagct 
     7201 gacttgtctg gttacactca cgatggttca ttcgaacaat acgctactgc tgatgctgtc 
     7261 caagccgcta aaattccagc tggtactgat ttagccaatg tcgcaccaat cttatgtgct 
     7321 ggtgttactg tttacaaagc cttaaagact gctgacttag cagctggcca atgggttgct 
     7381 atctccggtg ctggtggtgg tttaggttct ttggccgttc aatacgccag agccatgggt 
     7441 ttgagagttg tcgctattga cggtggtgac gaaaaaggtg aatttgttaa atcattgggt 
     7501 gctgaagctt acgttgattt caccaaagat aaagatattg ttgaagctgt caagaaagct 
     7561 actgatggtg gtccacacgg tgctatcaat gtctctgtct cagaaagagc cattggtcaa 
     7621 tccactgaat atgtcagaac tttaggtaaa gttgttttgg ttggtttgcc agctggtact 
     7681 aaaattagta ccccagtctt tgatgctgtt atcaagacca ttcaaattaa aggttcttat 
     7741 gtcggtaaca gaaaagatac tgctgaagcc gttgatttct tcacaagagg tttgatcaaa 
     7801 tgcccaatca agattgtcgg gcccggtacc cagcttttgt tccctttagt gagggttaat 
     7861 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     7921 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     7981 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     8041 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     8101 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     8161 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     8221 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     8281 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     8341 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     8401 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     8461 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     8521 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     8581 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     8641 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     8701 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     8761 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     8821 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     8881 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     8941 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     9001 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     9061 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     9121 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     9181 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     9241 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     9301 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     9361 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     9421 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     9481 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     9541 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     9601 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     9661 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     9721 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     9781 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     9841 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     9901 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     9961 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
    10021 aaaagtgcca c 
//
