LOCUS       pNIM1R-tTA              8838 bp    DNA     circular     25-JAN-2019
DEFINITION  LMBP 10514.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10514
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 25-JAN-2019
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12122642|
COMMENT     VNTDBDATE|-12657355|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pNIM1R-tTA|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pNIM1R-tTA" 0 8838 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "EcoRI" "NdeI" "PvuI" "SacII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 8838 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 4693 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 6565 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 1793 0 2574 0 3102 0 5183 
COMMENT            0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 502 0 8294 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 666 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 627 643 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "627..643") 
COMMENT        #6=(CFSignal (CObList) "T3 promoter" 30 0 1 6652 6668 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6652..6668)") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 6732 6815 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6732..6815)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 6907 7309 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6907..7309)") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7850 8710 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(7850..8710)") 
COMMENT        #10=(CFSignal (CObList) "f1 ori" 33 0 1 4 459 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4..459)") 
COMMENT        #11=(CFSignal (CObList) "CaRPS1 promoter" 29 0 0 668 1212 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "668..1212") 
COMMENT        #12=(CFSignal (CObList) "CatTA" 4 0 0 1213 2196 0 (CStringList) 
COMMENT             (CStringList "/gene=\"catTA\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"tetracycline regulated transactivator\"" 
COMMENT              "/protein_id=\"ART66063.1\"") 1 1 1 1 "1213..2196") 
COMMENT        #13=(CFSignal (CObList) "CaQCR7 bidirectional terminator" 43 0 0 2220 
COMMENT             2541 0 (CStringList) 
COMMENT             (CStringList "/regulatory_class=\"terminator\"") 1 1 1 1 
COMMENT             "2220..2541") 
COMMENT        #14=(CFSignal (CObList #15=(CLocSeg 0 562) #16=(CLocSeg 1217 9)) 
COMMENT             "nourseothricin resistance" 4 0 1 2578 3804 0 (CStringList) 
COMMENT             (CStringList "/gene=\"CaSAT1\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"nourseothricin resistance marker\"" 
COMMENT              "/protein_id=\"ART66064.1\"") 1 1 1 1 
COMMENT             "complement(2578..3140,3795..3804)") 
COMMENT        #17=(CFSignal (CObList) "CaACT1 promoter" 29 0 1 3805 4302 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(3805..4302)") 
COMMENT        #18=(CFSignal (CObList) "CaACT1 terminator" 43 0 1 4424 4691 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "complement(4424..4691)") 
COMMENT        #19=(CFSignal (CObList) "CaGFP" 4 0 1 4698 5414 0 (CStringList) 
COMMENT             (CStringList "/gene=\"GFP\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"green fluorescent protein\"" 
COMMENT              "/protein_id=\"ART66065.1\"") 1 1 1 1 "complement(4698..5414)") 
COMMENT        #20=(CFSignal (CObList) "CaOPS4T promoter" 29 0 1 5425 6079 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(5425..5790)") 
COMMENT        #21=(CFSignal (CObList) "CaRPS1 terminator" 43 0 0 6112 6614 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "6112..6614") 
COMMENT        #22=(CFSignal (CObList) "tetO" 21 0 1 6061 6079 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "tetO" 21 0 1 6019 6037 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "tetO" 21 0 1 5977 5995 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "tetO" 21 0 1 5935 5953 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "tetO" 21 0 1 5893 5911 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "tetO" 21 0 1 5851 5869 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #28=(CFSignal (CObList) "tetO" 21 0 1 5809 5827 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #29=(CFSignal (CObList) "LMBP 10514" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #30=(CFSignal (CObList) "Last modified: 25-JAN-2019" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA Plasmid Phage 'pNIM1R-tTA'" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP 10514." 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/19/18 10:17" 1) 
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COMMENT                          "Last Modified: 01/25/19 06:10" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8838 bp" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Modification Date in the Original DB: 15-JAN-2019" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  catTA;  CaSAT1;  GFP" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Ghent University" 1) 
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COMMENT                          "Technologiepark 927" 1) 
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COMMENT                          "B-9052 Gent-Zwijnaarde Belgium" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
COMMENT                          1) 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
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COMMENT                                 "" 1))) "LMBP 10514") 
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COMMENT                                   "CaOPS4T promoter"))) 
COMMENT                           "Promoter Eukaryotic (3 total)") 
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COMMENT              1.6976e-313 8.19697e-277) 
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FEATURES             Location/Qualifiers
     promoter        627..643
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(6652..6668)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(6732..6815)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(6907..7309)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7850..8710)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4..459)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        668..1212
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaRPS1\promoter
     CDS             1213..2196
                     /gene="catTA"
                     /codon_start=1
                     /transl_table=12
                     /product="tetracycline regulated transactivator"
                     /protein_id="ART66063.1"
                     /vntifkey="4"
                     /label=CatTA
     terminator      2220..2541
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaQCR7\bidirectional\terminator
     CDS             complement(2578..3140,3795..3804)
                     /gene="CaSAT1"
                     /codon_start=1
                     /transl_table=12
                     /product="nourseothricin resistance marker"
                     /protein_id="ART66064.1"
                     /vntifkey="4"
                     /label=nourseothricin\resistance
     promoter        complement(3805..4302)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaACT1\promoter
     terminator      complement(4424..4691)
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaACT1\terminator
     CDS             complement(4698..5414)
                     /gene="GFP"
                     /codon_start=1
                     /transl_table=12
                     /product="green fluorescent protein"
                     /protein_id="ART66065.1"
                     /vntifkey="4"
                     /label=CaGFP
     promoter        complement(5425..6079)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaOPS4T\promoter
     terminator      6112..6614
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaRPS1\terminator
     misc_feature    complement(6061..6079)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6019..6037)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(5977..5995)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(5935..5953)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(5893..5911)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(5851..5869)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(5809..5827)
                     /vntifkey="21"
                     /label=tetO
BASE COUNT     2689 a      1720 c      1797 g      2632 t 
ORIGIN
        1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
      601 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc 
      661 accgcggtca gtagatgatt tgttgttgtc gtttgatttt gttacagcgt aaccagtgcg 
      721 ttttgtttgt ttccacatca tacacttcac tgaaactaaa taagtttgtt tacattttga 
      781 gacttcaggt acgacccagg gttgcgacaa agtttaggta gtttgtcgtc tgaatgtcgc 
      841 aacaaaatag ggctgtagcc ctagtcatgt gatgtgaatt aacataacaa gaagaattgc 
      901 tggtgcgcaa aaagattatg tgtattttat gtgcgttgtt atcctgcaca ctaaaattga 
      961 gcagtgtaca cacacacata ttgggctgta tttttatttt tgtttttctg ctgttctctc 
     1021 actgttaagc tctaagtgaa tttgtgtgtg ctgtaatagt gtgtgtgttc caagtcccag 
     1081 ctctcacaga tactcacgca cgcccatact actgaaaatt tcctgacttt ctgtatctaa 
     1141 aaatttttta ctaggaattt ttttctttta cgtttttcac ttgtttcata taatcaccaa 
     1201 ctcaagtaca acatgtctag attagataaa agtaaagtga ttaacagcgc attagagttg 
     1261 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta 
     1321 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagca 
     1381 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa 
     1441 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga 
     1501 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 
     1561 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgca 
     1621 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa 
     1681 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 
     1741 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 
     1801 ttagaaaaac aacttaaatg tgaaagtggg tcctcgactt tgtttggtgg cgccaatttt 
     1861 aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac 
     1921 ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc 
     1981 tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt 
     2041 aataattcaa aaccattgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga 
     2101 atcactacag ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat 
     2161 gatgaagata ccccaccaaa cccaaaaaaa gagtaaggat ctaaattctg gaaatctaag 
     2221 taagtaagag gaggaggagg aggaagaaga tgagaaggtc aaacattgaa ataggaaaca 
     2281 agaaagcagg agacaagttt aaagaatgaa taataaatat tcttatgaaa gttatattta 
     2341 tttttgttag ttttctttta aatatattcg taatgtcaat ataaaacaat ttacaaaaag 
     2401 agattgtcaa aaagaagaaa cacatattat aatctaagtc tattaaatat ctctataaaa 
     2461 aaataatgtt atacttgaac aaaagcacta ttctttttgt ttatctatat ctatatatct 
     2521 atctatcaaa tatctttgtt tttaatctga tcgactcatt cctaatttaa acatatgtta 
     2581 ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt tcgagacttg 
     2641 aggtctagtt ttatacgtga agaggtcaat gccgccgaga gtaaagccac attttgcgta 
     2701 caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa ggagctgtct 
     2761 gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt tgcctcggtg 
     2821 cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg agttgagttc 
     2881 aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt cttcatcaga 
     2941 gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag atagttcaaa 
     3001 gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt tctcagcatc 
     3061 caatgtttcc gccacctgct cagggatcac cgaaattttc atatgagaac cgttatcgat 
     3121 aactaaagca gcaacttctt ctataaaaat gggttagtat gacagtcatt taaataagga 
     3181 atttttcagt tggcttggtt tcaattcaat gttcgttttt tttttttctt gctgtgtttg 
     3241 tgtttgtgtt gtttatagtt gtgtgcactg atcgtcgaaa aaaaaaattc atagtgagcc 
     3301 gggaaatctg tatagcccag ataacaacac aagtccaaac tagaaactcg tcaaacacca 
     3361 aaagcaatgt tgaatcaatt gccttgcaca agtacacgta ggaaaacata aaacattgca 
     3421 attttgaata ttgagccttt tgtcgtaaca ttgattgata ggattactca ccgaatggtt 
     3481 ttgaaaccac tgccgacaga tcaatcaatc aatcaaaaaa cgtgaacttt gaaaaagggg 
     3541 aagaacagat acattgaagt tagccatttc cactgatcgt cacaacatat ctgataaatt 
     3601 actttcaaaa ttataagctg atgtgtgtgt attattaatg tgacagtaac atcccaaacg 
     3661 agaaatatta tctcgacaac aaaaaagttt gatctgaatt gaaaatgaag ttttcccacc 
     3721 ctacccattt gtcatattga aaccaatcaa ctgattaatc aatcaattag aattgaagct 
     3781 aaactaaaac ataccaccgt ccattttgaa tgattatatt tttttaatat taatatcgag 
     3841 ataatgtttc taagaaagaa agaaaaccag gagtgaaaat tagaaaagga aaggaaagga 
     3901 aaaaaagaaa aatctgaaaa tatataaaaa aaaattgttt cgttggcaat aaatcttggt 
     3961 gagaacagcg accgaaagca aataagaaca aaatatgagt gtattacgtt gaacaactaa 
     4021 ttaacgtgtg tgtatggatc tttttttctt ttttctcttt aaccgactat aaacaacaaa 
     4081 catttttggg cagtgcacac actacttaat atacacagca taaattacac gattagaaac 
     4141 aaattagctt attaaaataa cctaatcaaa ccgaatattt tatggtatta tgagtaaact 
     4201 atataatata aatagcacac acccacaaca acaacaaagg aaaactaaaa ggttttttct 
     4261 ttttgaaaag atcgttttct ttattattct ctagttttga cgctcgacat tttatgatgg 
     4321 aatgaatggg atgaatcatc aaacaagaga aaatacccgt gacgaaaata ataaaataag 
     4381 ttcctctgat acagaagatg aaaacaacaa caacaagata tagaaatgcc ttgggtggct 
     4441 attttatagt cttaactttt taatgtatat ttgttttgtt tttttacata ataatacttt 
     4501 ataaaagcta agctaaattc aagtaaaatt tcaatctctc aaataaaaca tttttctctt 
     4561 tttcttaaat ttagttttat atatttataa aatatacaaa gattttttta aaaaagtaac 
     4621 aagttatata tgtaataaca aaaagaagaa taacaagaat acaaaaccag atttccagat 
     4681 ttccagaatt tagatcttta tttgtatagt tcatccatgc catgtgtaat cccagcagct 
     4741 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca 
     4801 gattgtgtgg acaagtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta 
     4861 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg 
     4921 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta 
     4981 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt 
     5041 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag 
     5101 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc 
     5161 ttgaaaaagt catgccgttt catatgatct gggtatctcg caaagcattg aagaccatac 
     5221 gcgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat 
     5281 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg 
     5341 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct 
     5401 tctcccttac tcatctttgt cgactattta tatttgtatg tgtgtaggag ttaacgtagt 
     5461 agtagacgta gttggtttta ttgtagttga tattgatttc tttctgagaa gttgaaagag 
     5521 tttgttgatt tgatttgata atgatataaa agaaataaaa aaaaatctaa aacttgaata 
     5581 caagagattt gaatctatat ttatatttca gaaaagccca tcagaaacga caaacatgga 
     5641 caacacttta attgaaaggg ctttattttt aagtgaagct ttttccttaa atcattgtta 
     5701 gtaattccca tagttattga atctattact caatcgagta gtttatattt aatttatcga 
     5761 caatattgcg aaaaataaaa aagaagtttg gagctcgact ttcacttttc tctatcactg 
     5821 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc 
     5881 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta 
     5941 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt 
     6001 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt 
     6061 tctctatcac tgatagggag tggtaaactc gacaaggtgc tgaaccaaac tggataatga 
     6121 taactgaaga gaagaattag ttttttcaag tgtataatat agtttctctc tattaccttt 
     6181 tccaataata gcattttaag ttttctattt tattttgtat aaaaaacata atgaaaaata 
     6241 cgtataagta atataaatga gtgtgggatt aagtgaatac gagatgttgt agtgataata 
     6301 ggggaaactc tttggcgaaa ctacaagaga gagtgatgtg ttaataatga acgaagaaat 
     6361 atgtgatttt tgtatgaaat ttgcaattat tctgattgaa tttgggtact ttcacattga 
     6421 atctagaacg actatacaaa tgtgctactt tgtcaaaata tcctttttga gaatcggcat 
     6481 atttatggcc ctgaatatcg actaccacat tccttttaca acactacgta accttttgag 
     6541 aaagtacaag tgaaagaagt atagaattca gtgtttagtt taacgtaagt attactgtgg 
     6601 aatgctttct tcgctcaaga ttgtcgggcc cggtacccag cttttgttcc ctttagtgag 
     6661 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 
     6721 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 
     6781 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 
     6841 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     6901 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     6961 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 
     7021 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     7081 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     7141 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     7201 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     7261 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     7321 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     7381 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     7441 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     7501 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 
     7561 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     7621 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     7681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     7741 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     7801 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     7861 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     7921 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     7981 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     8041 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     8101 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     8161 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     8221 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     8281 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     8341 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     8401 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     8461 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     8521 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     8581 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     8641 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     8701 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     8761 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     8821 ttccccgaaa agtgccac 
//
