ID pNIM1R-tTA standard; circular DNA; ; 8838 BP. DT 25-JAN-2019 DE LMBP 10514. CC Accession number: LMBP 10514 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 25-JAN-2019 CC CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|-12122642| CC VNTDBDATE|-12657355| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71| CC VNTOAUTHORAD3|B-9052 Gent Belgium| CC VNTREPLTYPE|Plasmid CC VNTREPLTYPE|Phage CC VNTEXTCHREPL|Bacteria FH Key Location/Qualifiers FH FT promoter 627..643 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(6652..6668) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(6732..6815) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(6907..7309) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7850..8710) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(4..459) FT /vntifkey="33" FT /label=f1\ori FT promoter 668..1212 FT /regulatory_class="promoter" FT /vntifkey="29" FT /label=CaRPS1\promoter FT CDS 1213..2196 FT /gene="catTA" FT /codon_start=1 FT /transl_table=12 FT /product="tetracycline regulated transactivator" FT /protein_id="ART66063.1" FT /vntifkey="4" FT /label=CatTA FT terminator 2220..2541 FT /regulatory_class="terminator" FT /vntifkey="43" FT /label=CaQCR7\bidirectional\terminator FT CDS complement(2578..3140,3795..3804) FT /gene="CaSAT1" FT /codon_start=1 FT /transl_table=12 FT /product="nourseothricin resistance marker" FT /protein_id="ART66064.1" FT /vntifkey="4" FT /label=nourseothricin\resistance FT promoter complement(3805..4302) FT /regulatory_class="promoter" FT /vntifkey="29" FT /label=CaACT1\promoter FT terminator complement(4424..4691) FT /regulatory_class="terminator" FT /vntifkey="43" FT /label=CaACT1\terminator FT CDS complement(4698..5414) FT /gene="GFP" FT /codon_start=1 FT /transl_table=12 FT /product="green fluorescent protein" FT /protein_id="ART66065.1" FT /vntifkey="4" FT /label=CaGFP FT promoter complement(5425..6079) FT /regulatory_class="promoter" FT /vntifkey="29" FT /label=CaOPS4T\promoter FT terminator 6112..6614 FT /regulatory_class="terminator" FT /vntifkey="43" FT /label=CaRPS1\terminator FT misc_feature complement(6061..6079) FT /vntifkey="21" FT /label=tetO FT misc_feature complement(6019..6037) FT /vntifkey="21" FT /label=tetO FT misc_feature complement(5977..5995) FT /vntifkey="21" FT /label=tetO FT misc_feature complement(5935..5953) FT /vntifkey="21" FT /label=tetO FT misc_feature complement(5893..5911) FT /vntifkey="21" FT /label=tetO FT misc_feature complement(5851..5869) FT /vntifkey="21" FT /label=tetO FT misc_feature complement(5809..5827) FT /vntifkey="21" FT /label=tetO SQ Sequence 8838 BP; 2689 A; 1720 C; 1797 G; 2632 t; ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 60 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 120 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 180 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 240 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 300 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 360 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 420 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 480 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 540 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 600 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc 660 accgcggtca gtagatgatt tgttgttgtc gtttgatttt gttacagcgt aaccagtgcg 720 ttttgtttgt ttccacatca tacacttcac tgaaactaaa taagtttgtt tacattttga 780 gacttcaggt acgacccagg gttgcgacaa agtttaggta gtttgtcgtc tgaatgtcgc 840 aacaaaatag ggctgtagcc ctagtcatgt gatgtgaatt aacataacaa gaagaattgc 900 tggtgcgcaa aaagattatg tgtattttat gtgcgttgtt atcctgcaca ctaaaattga 960 gcagtgtaca cacacacata ttgggctgta tttttatttt tgtttttctg ctgttctctc 1020 actgttaagc tctaagtgaa tttgtgtgtg ctgtaatagt gtgtgtgttc caagtcccag 1080 ctctcacaga tactcacgca cgcccatact actgaaaatt tcctgacttt ctgtatctaa 1140 aaatttttta ctaggaattt ttttctttta cgtttttcac ttgtttcata taatcaccaa 1200 ctcaagtaca acatgtctag attagataaa agtaaagtga ttaacagcgc attagagttg 1260 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta 1320 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagca 1380 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa 1440 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga 1500 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 1560 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgca 1620 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa 1680 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 1740 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 1800 ttagaaaaac aacttaaatg tgaaagtggg tcctcgactt tgtttggtgg cgccaatttt 1860 aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac 1920 ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc 1980 tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt 2040 aataattcaa aaccattgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga 2100 atcactacag ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat 2160 gatgaagata ccccaccaaa cccaaaaaaa gagtaaggat ctaaattctg gaaatctaag 2220 taagtaagag gaggaggagg aggaagaaga tgagaaggtc aaacattgaa ataggaaaca 2280 agaaagcagg agacaagttt aaagaatgaa taataaatat tcttatgaaa gttatattta 2340 tttttgttag ttttctttta aatatattcg taatgtcaat ataaaacaat ttacaaaaag 2400 agattgtcaa aaagaagaaa cacatattat aatctaagtc tattaaatat ctctataaaa 2460 aaataatgtt atacttgaac aaaagcacta ttctttttgt ttatctatat ctatatatct 2520 atctatcaaa tatctttgtt tttaatctga tcgactcatt cctaatttaa acatatgtta 2580 ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt tcgagacttg 2640 aggtctagtt ttatacgtga agaggtcaat gccgccgaga gtaaagccac attttgcgta 2700 caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa ggagctgtct 2760 gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt tgcctcggtg 2820 cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg agttgagttc 2880 aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt cttcatcaga 2940 gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag atagttcaaa 3000 gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt tctcagcatc 3060 caatgtttcc gccacctgct cagggatcac cgaaattttc atatgagaac cgttatcgat 3120 aactaaagca gcaacttctt ctataaaaat gggttagtat gacagtcatt taaataagga 3180 atttttcagt tggcttggtt tcaattcaat gttcgttttt tttttttctt gctgtgtttg 3240 tgtttgtgtt gtttatagtt gtgtgcactg atcgtcgaaa aaaaaaattc atagtgagcc 3300 gggaaatctg tatagcccag ataacaacac aagtccaaac tagaaactcg tcaaacacca 3360 aaagcaatgt tgaatcaatt gccttgcaca agtacacgta ggaaaacata aaacattgca 3420 attttgaata ttgagccttt tgtcgtaaca ttgattgata ggattactca ccgaatggtt 3480 ttgaaaccac tgccgacaga tcaatcaatc aatcaaaaaa cgtgaacttt gaaaaagggg 3540 aagaacagat acattgaagt tagccatttc cactgatcgt cacaacatat ctgataaatt 3600 actttcaaaa ttataagctg atgtgtgtgt attattaatg tgacagtaac atcccaaacg 3660 agaaatatta tctcgacaac aaaaaagttt gatctgaatt gaaaatgaag ttttcccacc 3720 ctacccattt gtcatattga aaccaatcaa ctgattaatc aatcaattag aattgaagct 3780 aaactaaaac ataccaccgt ccattttgaa tgattatatt tttttaatat taatatcgag 3840 ataatgtttc taagaaagaa agaaaaccag gagtgaaaat tagaaaagga aaggaaagga 3900 aaaaaagaaa aatctgaaaa tatataaaaa aaaattgttt cgttggcaat aaatcttggt 3960 gagaacagcg accgaaagca aataagaaca aaatatgagt gtattacgtt gaacaactaa 4020 ttaacgtgtg tgtatggatc tttttttctt ttttctcttt aaccgactat aaacaacaaa 4080 catttttggg cagtgcacac actacttaat atacacagca taaattacac gattagaaac 4140 aaattagctt attaaaataa cctaatcaaa ccgaatattt tatggtatta tgagtaaact 4200 atataatata aatagcacac acccacaaca acaacaaagg aaaactaaaa ggttttttct 4260 ttttgaaaag atcgttttct ttattattct ctagttttga cgctcgacat tttatgatgg 4320 aatgaatggg atgaatcatc aaacaagaga aaatacccgt gacgaaaata ataaaataag 4380 ttcctctgat acagaagatg aaaacaacaa caacaagata tagaaatgcc ttgggtggct 4440 attttatagt cttaactttt taatgtatat ttgttttgtt tttttacata ataatacttt 4500 ataaaagcta agctaaattc aagtaaaatt tcaatctctc aaataaaaca tttttctctt 4560 tttcttaaat ttagttttat atatttataa aatatacaaa gattttttta aaaaagtaac 4620 aagttatata tgtaataaca aaaagaagaa taacaagaat acaaaaccag atttccagat 4680 ttccagaatt tagatcttta tttgtatagt tcatccatgc catgtgtaat cccagcagct 4740 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca 4800 gattgtgtgg acaagtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta 4860 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg 4920 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta 4980 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt 5040 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag 5100 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc 5160 ttgaaaaagt catgccgttt catatgatct gggtatctcg caaagcattg aagaccatac 5220 gcgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat 5280 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg 5340 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct 5400 tctcccttac tcatctttgt cgactattta tatttgtatg tgtgtaggag ttaacgtagt 5460 agtagacgta gttggtttta ttgtagttga tattgatttc tttctgagaa gttgaaagag 5520 tttgttgatt tgatttgata atgatataaa agaaataaaa aaaaatctaa aacttgaata 5580 caagagattt gaatctatat ttatatttca gaaaagccca tcagaaacga caaacatgga 5640 caacacttta attgaaaggg ctttattttt aagtgaagct ttttccttaa atcattgtta 5700 gtaattccca tagttattga atctattact caatcgagta gtttatattt aatttatcga 5760 caatattgcg aaaaataaaa aagaagtttg gagctcgact ttcacttttc tctatcactg 5820 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc 5880 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta 5940 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt 6000 aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt 6060 tctctatcac tgatagggag tggtaaactc gacaaggtgc tgaaccaaac tggataatga 6120 taactgaaga gaagaattag ttttttcaag tgtataatat agtttctctc tattaccttt 6180 tccaataata gcattttaag ttttctattt tattttgtat aaaaaacata atgaaaaata 6240 cgtataagta atataaatga gtgtgggatt aagtgaatac gagatgttgt agtgataata 6300 ggggaaactc tttggcgaaa ctacaagaga gagtgatgtg ttaataatga acgaagaaat 6360 atgtgatttt tgtatgaaat ttgcaattat tctgattgaa tttgggtact ttcacattga 6420 atctagaacg actatacaaa tgtgctactt tgtcaaaata tcctttttga gaatcggcat 6480 atttatggcc ctgaatatcg actaccacat tccttttaca acactacgta accttttgag 6540 aaagtacaag tgaaagaagt atagaattca gtgtttagtt taacgtaagt attactgtgg 6600 aatgctttct tcgctcaaga ttgtcgggcc cggtacccag cttttgttcc ctttagtgag 6660 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6720 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6780 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6840 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6900 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6960 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7020 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7080 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7140 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7200 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7260 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7320 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7380 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7440 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7500 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7560 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7620 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7680 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7740 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7800 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7860 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7920 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7980 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8040 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8100 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8160 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8220 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8280 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8340 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8400 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8460 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8520 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8580 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8640 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8700 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8760 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8820 ttccccgaaa agtgccac 8838 //