LOCUS       pNIM1CtR-tTA            8818 bp    DNA     circular     19-JAN-2018
DEFINITION  LMBP 10516.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12122654|
COMMENT     VNTDBDATE|-12122654|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pNIM1CtR-tTA|

COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pNIM1CtR-tTA" 0 8818 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "SacI" "KpnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 654 659 1 8818 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 659 0 5801 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 6617 0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 30 0 0 627 643 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "627..643") 
COMMENT        #3=(CFSignal (CObList) "T3 promoter" 30 0 1 6632 6648 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6651..6667)") 
COMMENT        #4=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 21 0 1 6674 6711 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6693..6730)") 
COMMENT        #5=(CFSignal (CObList) "lac promoter" 30 0 1 6712 6795 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6731..6814)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 6887 7289 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6906..7308)") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7830 8690 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(7849..8709)") 
COMMENT        #8=(CFSignal (CObList) "f1 ori" 33 0 1 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4..459)") 
COMMENT        #9=(CFSignal (CObList) "catTA" 4 0 0 1218 2201 0 (CStringList) 
COMMENT            (CStringList "/gene=\"catTA\"" "/codon_start=1" "/transl_table=12" 
COMMENT             "/product=\"tetracycline regulated transactivator\"" 
COMMENT             "/protein_id=\"ART66069.1\"") 1 1 1 1 "565..1548") 
COMMENT        #10=(CFSignal (CObList #11=(CLocSeg 0 562) #12=(CLocSeg 1217 9)) 
COMMENT             "CaSAT1" 4 0 1 2583 3809 0 (CStringList) 
COMMENT             (CStringList "/gene=\"CaSAT1\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"nourseothricin resistance marker\"" 
COMMENT              "/protein_id=\"ART66070.1\"") 1 1 1 1 
COMMENT             "complement(join(1930..2492,3147..3156))") 
COMMENT        #13=(CFSignal (CObList) "GFP" 4 0 1 4703 5419 0 (CStringList) 
COMMENT             (CStringList "/gene=\"GFP\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"green fluorescent protein\"" 
COMMENT              "/protein_id=\"ART66071.1\"") 1 1 1 1 "complement(4050..4766)") 
COMMENT        #14=(CFSignal (CObList) "CtRPS10 promoter" 29 0 0 668 1217 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "15..564") 
COMMENT        #15=(CFSignal (CObList) "CaQCR7 terminator" 43 0 0 2245 2576 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "1592..1923") 
COMMENT        #16=(CFSignal (CObList) "CaACT1 promoter" 29 0 1 3810 4307 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(3157..3654)") 
COMMENT        #17=(CFSignal (CObList) "CaACT1 terminator" 43 0 1 4314 4696 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "complement(3661..4043)") 
COMMENT        #18=(CFSignal (CObList) "CaOP4 promoter" 29 0 1 5430 5795 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(4777..5142)") 
COMMENT        #19=(CFSignal (CObList) "tet operator" 29 0 1 5805 6098 0 (CStringList) 
COMMENT             (CStringList "/regulatory_class=\"promoter\"") 1 1 1 1 
COMMENT             "complement(5152..5445)") 
COMMENT        #20=(CFSignal (CObList) "CtRPS10 terminator" 43 0 0 6118 6594 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "5465..5941")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #21=(CFSignal (CObList) "LMBP 10516" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA Plasmid Phage 'pNIM1CtR-tTA'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP 10516." 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/19/18 10:29" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/19/18 10:29" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8818 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 18-JAN-2018" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  cartTA;  CaSAT1;  GFP" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 10516"))) "Annotations") 
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COMMENT                                        "   /codon_start=1" 1) 
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COMMENT                                        "   complement(7849..8709)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (4 total)") 
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COMMENT                                   #81=(CLinePar 
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COMMENT                                 "5' UTR of lacZ, incl. RBS"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                 "CtRPS10 promoter") 
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COMMENT                                 "CaACT1 promoter") 
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COMMENT                                 "CaOP4 promoter") 
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COMMENT                                    #106=(CLinePar 
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COMMENT                                  "tet operator"))) 
COMMENT                          "Promoter Eukaryotic (4 total)") 
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COMMENT                                           "   complement(6731..6814)" 1))) 
COMMENT                                   "lac promoter"))) 
COMMENT                           "Promoter Prokaryotic (3 total)") 
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COMMENT                                           "   complement(6906..7308)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (2 total)") 
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FEATURES             Location/Qualifiers
     promoter        627..643
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(6632..6648)
                     /vntifkey="30"
                     /label=T3\promoter
     misc_feature    complement(6674..6711)
                     /vntifkey="21"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(6712..6795)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(6887..7289)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(7830..8690)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4..459)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        668..1217
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CtRPS10\promoter
     CDS             1218..2201
                     /gene="catTA"
                     /codon_start=1
                     /transl_table=12
                     /product="tetracycline regulated transactivator"
                     /protein_id="ART66069.1"
                     /vntifkey="4"
                     /label=catTA
     terminator      2245..2576
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaQCR7\terminator
     CDS             complement(2583..3145,3800..3809)
                     /gene="CaSAT1"
                     /codon_start=1
                     /transl_table=12
                     /product="nourseothricin resistance marker"
                     /protein_id="ART66070.1"
                     /vntifkey="4"
                     /label=CaSAT1
     promoter        complement(3810..4307)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaACT1\promoter
     terminator      complement(4314..4696)
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaACT1\terminator
     CDS             complement(4703..5419)
                     /gene="GFP"
                     /codon_start=1
                     /transl_table=12
                     /product="green fluorescent protein"
                     /protein_id="ART66071.1"
                     /vntifkey="4"
                     /label=GFP
     promoter        complement(5430..5795)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaOP4\promoter
     promoter        complement(5805..6098)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=tet\operator
     terminator      6118..6594
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CtRPS10\terminator
BASE COUNT     2659 a      1734 c      1775 g      2650 t 
ORIGIN
        1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
      601 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc 
      661 accgcggttt ctcttgatgc actttgttta cattttgata ttaaggtact accgttcgac 
      721 gcgacatttt attgtcgtca aaatgtcgca caacaattta gggttttagc cctgctcacg 
      781 tgtatttatt ttgttttttg tagtgaaatt tagtttgtat taattatatc ctgtatattg 
      841 atgttttggt actttgcgca cactaatttg ttagtgaatt ttgaacgatt caacaatctt 
      901 ttttctgtcc aacgatagga gtgcgtatgg acgtagtcgc gcggtggtgg cccctttgtt 
      961 ctggtgggac tctcgcccga ttttacagat tctggcagtc gcacccacac attctgtgtc 
     1021 tctctcacat tgagtcttgt ctctcagctg gtcgcacacg cattgtctct cttactctca 
     1081 ttctcccaac tctcgctttc ccaatactag tgaaattttt ttttttttca attgcaagtg 
     1141 tatctcataa aaatttttta tgggattttt tttttctttc ttactttgtt taacttaatc 
     1201 accaactcaa gtacatcatg tctagattag ataaaagtaa agtgattaac agcgcattag 
     1261 agttgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 
     1321 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 
     1381 tagcaattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 
     1441 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 
     1501 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 
     1561 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 
     1621 gcgcagtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 
     1681 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 
     1741 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 
     1801 gcggattaga aaaacaactt aaatgtgaaa gtgggtcctc gactttgttt ggtggcgcca 
     1861 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta 
     1921 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg 
     1981 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg 
     2041 atggtaataa ttcaaaacca ttgtcacctg gttggacgga ccaaactgcg tataacgcgt 
     2101 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat 
     2161 tcgatgatga agatacccca ccaaacccaa aaaaagagta aggatctaaa ttctggaaat 
     2221 ctaagtaagt aagaggagga ggaggaggaa gaagatgaga aggtcaaaca ttgaaatagg 
     2281 aaacaagaaa gcaggagaca agtttaaaga atgaataata aatattctta tgaaagttat 
     2341 atttattttt gttagttttc ttttaaatat attcgtaatg tcaatataaa acaatttaca 
     2401 aaaagagatt gtcaaaaaga agaaacacat attataatct aagtctatta aatatctcta 
     2461 taaaaaaata atgttatact tgaacaaaag cactattctt tttgtttatc tatatctata 
     2521 tatctatcta tcaaatatct ttgtttttaa tctgatcgac tcattcctaa tttaaacata 
     2581 tgttaggcgt catcctgtgc tcccgagaac cagtaccagt acatcgctgt ttcgttcgag 
     2641 acttgaggtc tagttttata cgtgaagagg tcaatgccgc cgagagtaaa gccacatttt 
     2701 gcgtacaaat tgcaggcagg tacattgttc gtttgtgtct ctaatcgtat gccaaggagc 
     2761 tgtctgctta gtgcccactt tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct 
     2821 cggtgcgtgt gcgacacaac aatgtgttcg atagaggcta gatcgttcca tgttgagttg 
     2881 agttcaatct tcccgacaag ctcttggtcg atgaatgcgc catagcaagc agagtcttca 
     2941 tcagagtcat catccgagat gtaatccttc cggtaggggc tcacacttct ggtagatagt 
     3001 tcaaagcctt ggtcggatag gtgcacatcg aacacttcac gaacaatgaa atggttctca 
     3061 gcatccaatg tttccgccac ctgctcaggg atcaccgaaa ttttcatatg agaaccgtta 
     3121 tcgataacta aagcagcaac ttcttctata aaaatgggtt agtatgacag tcatttaaat 
     3181 aaggaatttt tcagttggct tggtttcaat tcaatgttcg tttttttttt ttcttgctgt 
     3241 gtttgtgttt gtgttgttta tagttgtgtg cactgatcgt cgaaaaaaaa aattcatagt 
     3301 gagccgggaa atctgtatag cccagataac aacacaagtc caaactagaa actcgtcaaa 
     3361 caccaaaagc aatgttgaat caattgcctt gcacaagtac acgtaggaaa acataaaaca 
     3421 ttgcaatttt gaatattgag ccttttgtcg taacattgat tgataggatt actcaccgaa 
     3481 tggttttgaa accactgccg acagatcaat caatcaatca aaaaacgtga actttgaaaa 
     3541 aggggaagaa cagatacatt gaagttagcc atttccactg atcgtcacaa catatctgat 
     3601 aaattacttt caaaattata agctgatgtg tgtgtattat taatgtgaca gtaacatccc 
     3661 aaacgagaaa tattatctcg acaacaaaaa agtttgatct gaattgaaaa tgaagttttc 
     3721 ccaccctacc catttgtcat attgaaacca atcaactgat taatcaatca attagaattg 
     3781 aagctaaact aaaacatacc accgtccatt ttgaatgatt atattttttt aatattaata 
     3841 tcgagataat gtttctaaga aagaaagaaa accaggagtg aaaattagaa aaggaaagga 
     3901 aaggaaaaaa agaaaaatct gaaaatatat aaaaaaaaat tgtttcgttg gcaataaatc 
     3961 ttggtgagaa cagcgaccga aagcaaataa gaacaaaata tgagtgtatt acgttgaaca 
     4021 actaattaac gtgtgtgtat ggatcttttt ttcttttttc tctttaaccg actataaaca 
     4081 acaaacattt ttgggcagtg cacacactac ttaatataca cagcataaat tacacgatta 
     4141 gaaacaaatt agcttattaa aataacctaa tcaaaccgaa tattttatgg tattatgagt 
     4201 aaactatata atataaatag cacacaccca caacaacaac aaaggaaaac taaaaggttt 
     4261 tttctttttg aaaagatcgt tttctttatt attctctagt tttgacgctc gacattttat 
     4321 gatggaatga atgggatgaa tcatcaaaca agagaaaata cccgtgacga aaataataaa 
     4381 ataagttcct ctgatacaga agatgaaaac aacaacaaca agatatagaa atgccttggg 
     4441 tggctatttt atagtcttaa ctttttaatg tatatttgtt ttgttttttt acataataat 
     4501 actttataaa agctaagcta aattcaagta aaatttcaat ctctcaaata aaacattttt 
     4561 ctctttttct taaatttagt tttatatatt tataaaatat acaaagattt ttttaaaaaa 
     4621 gtaacaagtt atatatgtaa taacaaaaag aagaataaca agaatacaaa accagatttc 
     4681 cagatttcca gaatttagat ctttatttgt atagttcatc catgccatgt gtaatcccag 
     4741 cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa 
     4801 gggcagattg tgtggacaag taatggttgt ctggtaaaag gacagggcca tcgccaattg 
     4861 gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa 
     4921 ttttgaagtt aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg 
     4981 agttatagtt gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac 
     5041 cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct 
     5101 tgtagttccc gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg 
     5161 cactcttgaa aaagtcatgc cgtttcatat gatctgggta tctcgcaaag cattgaagac 
     5221 catacgcgaa agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa 
     5281 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa 
     5341 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa 
     5401 gttcttctcc cttactcatc tttgtcgact atttatattt gtatgtgtgt aggagttaac 
     5461 gtagtagtag acgtagttgg ttttattgta gttgatattg atttctttct gagaagttga 
     5521 aagagtttgt tgatttgatt tgataatgat ataaaagaaa taaaaaaaaa tctaaaactt 
     5581 gaatacaaga gatttgaatc tatatttata tttcagaaaa gcccatcaga aacgacaaac 
     5641 atggacaaca ctttaattga aagggcttta tttttaagtg aagctttttc cttaaatcat 
     5701 tgttagtaat tcccatagtt attgaatcta ttactcaatc gagtagttta tatttaattt 
     5761 atcgacaata ttgcgaaaaa taaaaaagaa gtttggagct cgactttcac ttttctctat 
     5821 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta 
     5881 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt 
     5941 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga 
     6001 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc 
     6061 acttttctct atcactgata gggagtggta aactcgacaa ggtgctgaac caaactggag 
     6121 aatagcaaga ataattaata gaaggtattt ctgtttattt aatgtacaat ataggttctc 
     6181 tttttagcat tttctttttg gtttctattt tattttgtat aaaaacataa tgaaaaatac 
     6241 atataagtaa tataaaaaaa aattaaagtg tttggggtta ccaataggaa tgtagtttgg 
     6301 tctctttggt cttgagatgg gttatacaac aaacaccaac tataactaaa agatcgtgcg 
     6361 agtatatttg aattggaagt ttaatttttg tcaagtttcg caacattttc ccaataaaaa 
     6421 tcgaaagtgt taattcaata ttgtaattgc aatttgatat tcaattaaac cacgttaatt 
     6481 aggtattgca catcacagaa atgactctaa ttttgcttca tacaaacaat aggactatca 
     6541 agcgagatca ttgaacggtt ttacatcttt gctcaagctg aattttacga ggtgtcaaga 
     6601 ttgtcgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg 
     6661 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 
     6721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 
     6781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 
     6841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
     6901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     6961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     7021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     7081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     7141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     7201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     7261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     7321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     7381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     7441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     7501 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     7741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     7801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     7861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     7921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     7981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     8041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     8101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     8161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     8221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     8281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     8341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     8401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     8461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     8521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     8581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     8641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     8701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     8761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac 
//
